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The map label for this gene is outO [H]

Identifier: 238920385

GI number: 238920385

Start: 2391305

End: 2391964

Strand: Reverse

Name: outO [H]

Synonym: NT01EI_2495

Alternate gene names: 238920385

Gene position: 2391964-2391305 (Counterclockwise)

Preceding gene: 238920386

Following gene: 238920382

Centisome position: 62.74

GC content: 67.73

Gene sequence:

>660_bases
ATGGATCTGTGGCTGATGCTGGCGGCCCTGTGTGGCCTGGCGCTCGGTATGCCCCTGCAGTGCGCGATGCTGCGAATTAT
GCGCCCGCTCTGCTCCCCGCTGCTCTGGCACGATCGTCATTTCTCTCCTTTTCCCTGCCGTGCGGCGCTGTGTACCGCGC
TGGCGGCGATCGCCCTGTTGCTGGCCTGGCGCCTGCCGCCCGGCGCCGCCTGGCTCTGTGGGCTGAGCTTCTGCACGCTG
AGTCTTGCCCTGGCGCTGATCGATCGGGCCAGCCTGCTGTTGCCCGATGCGCTGACTCAGCCACTGCTGTGGCTGGGGCT
GATATGGAACGCGCTATATCAGCCGACGCATTTGCCACAGGCGGTGTTCGGTGCCGCAACAGGGTATCTGGCGCTATGGC
TGATCGCCTGGGGGTTCTGGCGCTGGCGCGGGGTGCAGGGGCTCGGCGGCGGAGATGTTAAGCTGCTGGCCGCGCTCGGC
GCCTGGTGCGGCGGGCCTGATCTGCCTGCGCTGTTGCTGATGGCATCCCTGCTGACGCTTGGCAGCATAGCCCTGCTGCA
CTGCTGGCGGGGAGTGAGTCTGGCGGAGCCCGTGCCATTTGGGCCAGCGCTGCTGGTGGCCGGGCTGTGGCAGGTGCTGC
CGCGTCTGGTCAGCGTCTAG

Upstream 100 bases:

>100_bases
GCCGGCGTGAGTCAGGTGCCTTTTCTGCTAAAGAATATGTTTTCGCGGGTGCTATGGCTGGCGCGCGGCAAGGCCGACTA
GCGCCCACCTGGCGGTGGCC

Downstream 100 bases:

>100_bases
CGCGACAAGACGATGCTATGCAGTCGCACCCAGGCTGGCGCATATCGCCTGCTGCATTTCATCCAGCAGCTGATAGCGGC
GATGGTACTCGGCGCGCTTT

Product: type IV prepilin leader peptidase

Products: NA

Alternate protein names: Pectic enzymes secretion protein outO; Leader peptidase; Prepilin peptidase; N-methyltransferase [H]

Number of amino acids: Translated: 219; Mature: 219

Protein sequence:

>219_residues
MDLWLMLAALCGLALGMPLQCAMLRIMRPLCSPLLWHDRHFSPFPCRAALCTALAAIALLLAWRLPPGAAWLCGLSFCTL
SLALALIDRASLLLPDALTQPLLWLGLIWNALYQPTHLPQAVFGAATGYLALWLIAWGFWRWRGVQGLGGGDVKLLAALG
AWCGGPDLPALLLMASLLTLGSIALLHCWRGVSLAEPVPFGPALLVAGLWQVLPRLVSV

Sequences:

>Translated_219_residues
MDLWLMLAALCGLALGMPLQCAMLRIMRPLCSPLLWHDRHFSPFPCRAALCTALAAIALLLAWRLPPGAAWLCGLSFCTL
SLALALIDRASLLLPDALTQPLLWLGLIWNALYQPTHLPQAVFGAATGYLALWLIAWGFWRWRGVQGLGGGDVKLLAALG
AWCGGPDLPALLLMASLLTLGSIALLHCWRGVSLAEPVPFGPALLVAGLWQVLPRLVSV
>Mature_219_residues
MDLWLMLAALCGLALGMPLQCAMLRIMRPLCSPLLWHDRHFSPFPCRAALCTALAAIALLLAWRLPPGAAWLCGLSFCTL
SLALALIDRASLLLPDALTQPLLWLGLIWNALYQPTHLPQAVFGAATGYLALWLIAWGFWRWRGVQGLGGGDVKLLAALG
AWCGGPDLPALLLMASLLTLGSIALLHCWRGVSLAEPVPFGPALLVAGLWQVLPRLVSV

Specific function: Cleaves type-4 fimbrial leader sequence and methylates the N-terminal (generally Phe) residue [H]

COG id: COG1989

COG function: function code NOU; Type II secretory pathway, prepilin signal peptidase PulO and related peptidases

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase A24 family [H]

Homologues:

Organism=Escherichia coli, GI1789732, Length=128, Percent_Identity=47.65625, Blast_Score=79, Evalue=2e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010627
- InterPro:   IPR014032
- InterPro:   IPR000045 [H]

Pfam domain/function: PF06750 DiS_P_DiS; PF01478 Peptidase_A24 [H]

EC number: =3.4.23.43 [H]

Molecular weight: Translated: 23557; Mature: 23557

Theoretical pI: Translated: 8.34; Mature: 8.34

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

4.1 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
6.8 %Cys+Met (Translated Protein)
4.1 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
6.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDLWLMLAALCGLALGMPLQCAMLRIMRPLCSPLLWHDRHFSPFPCRAALCTALAAIALL
CHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHH
LAWRLPPGAAWLCGLSFCTLSLALALIDRASLLLPDALTQPLLWLGLIWNALYQPTHLPQ
HHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCCCHH
AVFGAATGYLALWLIAWGFWRWRGVQGLGGGDVKLLAALGAWCGGPDLPALLLMASLLTL
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHH
GSIALLHCWRGVSLAEPVPFGPALLVAGLWQVLPRLVSV
HHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MDLWLMLAALCGLALGMPLQCAMLRIMRPLCSPLLWHDRHFSPFPCRAALCTALAAIALL
CHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHH
LAWRLPPGAAWLCGLSFCTLSLALALIDRASLLLPDALTQPLLWLGLIWNALYQPTHLPQ
HHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCCCHH
AVFGAATGYLALWLIAWGFWRWRGVQGLGGGDVKLLAALGAWCGGPDLPALLLMASLLTL
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHH
GSIALLHCWRGVSLAEPVPFGPALLVAGLWQVLPRLVSV
HHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 1429461 [H]