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The map label for this gene is pyrF [H]

Identifier: 238920240

GI number: 238920240

Start: 2244037

End: 2244759

Strand: Reverse

Name: pyrF [H]

Synonym: NT01EI_2349

Alternate gene names: 238920240

Gene position: 2244759-2244037 (Counterclockwise)

Preceding gene: 238920241

Following gene: 238920239

Centisome position: 58.88

GC content: 64.32

Gene sequence:

>723_bases
ATGCCAATATCTACTCCGATACGCTCCCATAGCCACTCTCCGATCGTTGTCGCCCTTGATTATGCCGATCGAAACGCCGC
CCTCGCACTGGTGGATGCGCTCGATCCGCGCGACTGCCGCCTGAAGGTCGGCAAGGAAATGTTCACCCTGTTCGGTCCCG
AGTGGGTGCGTACCCTGCAGCAGCGCGGATTCGATGTGTTTCTGGATCTTAAGTTTCACGATATCCCCAATACCGTCGCC
CGCGCCGTGGCGGCGGCGGCGGATCTGGGGGTATGGATGGTCAACGTGCACGCCAGCGGTAGCGTACGCATGATGAATGC
GGCCCGGGAGGCGCTGCTGCCCTACGGTAAGGAGGCGCCGCGGCTGATCGCCGTGACGGTGTTGACCAGTATGGAGCAGG
CCGATCTGCAGGCGATCGGCATCGATTTGGCGCCGGCTGACTATGCCGAGCGCCTGGCGCGCCTGACGCAGCAGTGTGGG
CTGGATGGCGTGGTCTGTTCGGCACAGGAGGCGGTGCGCATCAATGCCGTCTGTGGATCCGCCTTTACACTGGTGACGCC
GGGGATCCGGCCCGCAGGGAGTGAGGCTGGTGATCAGCGTCGGATTATGACGCCACAGCAGGCCGTCGCCGCCGGGGTTG
ACTATATGGTGATCGGGCGTCCAATCACCCAGGCGGCGCAGCCGGCCGAGGCGCTGCGCGCCATTCTGGCGACGCTGGCC
TAA

Upstream 100 bases:

>100_bases
GCGCGTCGTACCTACGCCCTAGGCAGAGCGGGGCGTGCTGTGTACAATGCCGCTCCTGAAAAAGGTATTTCTCCGCTAAC
GCCAGATTCAGGAACCCATC

Downstream 100 bases:

>100_bases
ATCGAGACGGGAGTGGGTAAAAATGCAAGAGAGTAACAGTCGGCTGGTCTACACCACCGACGCTGGGCGAGTCCACGAAA
CGGCGGCCGTAGCGTCACGT

Product: orotidine 5'-phosphate decarboxylase

Products: NA

Alternate protein names: OMP decarboxylase; OMPDCase; OMPdecase [H]

Number of amino acids: Translated: 240; Mature: 239

Protein sequence:

>240_residues
MPISTPIRSHSHSPIVVALDYADRNAALALVDALDPRDCRLKVGKEMFTLFGPEWVRTLQQRGFDVFLDLKFHDIPNTVA
RAVAAAADLGVWMVNVHASGSVRMMNAAREALLPYGKEAPRLIAVTVLTSMEQADLQAIGIDLAPADYAERLARLTQQCG
LDGVVCSAQEAVRINAVCGSAFTLVTPGIRPAGSEAGDQRRIMTPQQAVAAGVDYMVIGRPITQAAQPAEALRAILATLA

Sequences:

>Translated_240_residues
MPISTPIRSHSHSPIVVALDYADRNAALALVDALDPRDCRLKVGKEMFTLFGPEWVRTLQQRGFDVFLDLKFHDIPNTVA
RAVAAAADLGVWMVNVHASGSVRMMNAAREALLPYGKEAPRLIAVTVLTSMEQADLQAIGIDLAPADYAERLARLTQQCG
LDGVVCSAQEAVRINAVCGSAFTLVTPGIRPAGSEAGDQRRIMTPQQAVAAGVDYMVIGRPITQAAQPAEALRAILATLA
>Mature_239_residues
PISTPIRSHSHSPIVVALDYADRNAALALVDALDPRDCRLKVGKEMFTLFGPEWVRTLQQRGFDVFLDLKFHDIPNTVAR
AVAAAADLGVWMVNVHASGSVRMMNAAREALLPYGKEAPRLIAVTVLTSMEQADLQAIGIDLAPADYAERLARLTQQCGL
DGVVCSAQEAVRINAVCGSAFTLVTPGIRPAGSEAGDQRRIMTPQQAVAAGVDYMVIGRPITQAAQPAEALRAILATLA

Specific function: Catalyzes the decarboxylation of orotidine 5'- monophosphate (OMP) to uridine 5'-monophosphate (UMP) [H]

COG id: COG0284

COG function: function code F; Orotidine-5'-phosphate decarboxylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the OMP decarboxylase family. Type 1 subfamily [H]

Homologues:

Organism=Escherichia coli, GI1787537, Length=236, Percent_Identity=75.4237288135593, Blast_Score=361, Evalue=1e-101,

Paralogues:

None

Copy number: 6,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR014732
- InterPro:   IPR018089
- InterPro:   IPR001754
- InterPro:   IPR011060 [H]

Pfam domain/function: PF00215 OMPdecase [H]

EC number: =4.1.1.23 [H]

Molecular weight: Translated: 25655; Mature: 25524

Theoretical pI: Translated: 6.51; Mature: 6.51

Prosite motif: PS00156 OMPDECASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPISTPIRSHSHSPIVVALDYADRNAALALVDALDPRDCRLKVGKEMFTLFGPEWVRTLQ
CCCCCCCCCCCCCCEEEEEEECCCCCEEEEEECCCCCCHHHHHHHHHHHHCCHHHHHHHH
QRGFDVFLDLKFHDIPNTVARAVAAAADLGVWMVNVHASGSVRMMNAAREALLPYGKEAP
HCCCEEEEEEEECCCCHHHHHHHHHHHHCCEEEEEEECCCCEEHHHHHHHHHCCCCCCCC
RLIAVTVLTSMEQADLQAIGIDLAPADYAERLARLTQQCGLDGVVCSAQEAVRINAVCGS
CEEEEHHHHHHHHHHHHHEECCCCCHHHHHHHHHHHHHHCCCCEEECCCCCEEEEEECCC
AFTLVTPGIRPAGSEAGDQRRIMTPQQAVAAGVDYMVIGRPITQAAQPAEALRAILATLA
EEEEECCCCCCCCCCCCCCCCCCCHHHHHHCCCCEEEECCCHHHHCCHHHHHHHHHHHCC
>Mature Secondary Structure 
PISTPIRSHSHSPIVVALDYADRNAALALVDALDPRDCRLKVGKEMFTLFGPEWVRTLQ
CCCCCCCCCCCCCEEEEEEECCCCCEEEEEECCCCCCHHHHHHHHHHHHCCHHHHHHHH
QRGFDVFLDLKFHDIPNTVARAVAAAADLGVWMVNVHASGSVRMMNAAREALLPYGKEAP
HCCCEEEEEEEECCCCHHHHHHHHHHHHCCEEEEEEECCCCEEHHHHHHHHHCCCCCCCC
RLIAVTVLTSMEQADLQAIGIDLAPADYAERLARLTQQCGLDGVVCSAQEAVRINAVCGS
CEEEEHHHHHHHHHHHHHEECCCCCHHHHHHHHHHHHHHCCCCEEECCCCCEEEEEECCC
AFTLVTPGIRPAGSEAGDQRRIMTPQQAVAAGVDYMVIGRPITQAAQPAEALRAILATLA
EEEEECCCCCCCCCCCCCCCCCCCHHHHHHCCCCEEEECCCHHHHCCHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA