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The map label for this gene is purB [H]

Identifier: 238920212

GI number: 238920212

Start: 2218546

End: 2219916

Strand: Direct

Name: purB [H]

Synonym: NT01EI_2321

Alternate gene names: 238920212

Gene position: 2218546-2219916 (Clockwise)

Preceding gene: 238920211

Following gene: 238920213

Centisome position: 58.19

GC content: 59.59

Gene sequence:

>1371_bases
ATGGAATTATCCTCCCTGACCGCCGTATCCCCAATCGACGGCCGCTACGGCGAGAAAGTCAGTGCCCTGCGGCCGATCTT
TAGTGAGTTCGGTCTGCTGAAGTTTCGCGTCCAGGTAGAAGTACGTTGGCTGCAAAAACTGGCCGCTTGTGCAGAAATCG
GCGAAGTTCCCGCTTTTGATGCGGACGCAAACGCTTTCCTTGACCGGATCGCCGACCAGTTCAGCGAAGAAGATGCCCGC
CGTATCAAAACCATTGAGCGTACCACCAATCACGACGTGAAGGCGGTCGAGTATTTCCTGAAAGAAAAAGTGGCTGACCA
GCCTGCGCTGCACGCGGTCTCTGAGTTTATCCACTTCGCCTGCACCTCCGAGGATATCAACAACCTCTCCCATGCCCTGA
TGCTGAACAGCGCGCGCGAAGAGGTGCTTAAGCCGGTCTGGCGCCAGCTGATCGATACCCTGCGCGATCTGGCGCAGCGC
TACCGCGATATTCCACTGATGGCGCGTACCCACGGCCAGCCGGCCACCCCCTCGACCGTCGGCAAGGAGTTTGCCAACGT
CGCCTACCGTCTGGAGCGCCAGTTCCGTCAGCTGACGCAGGTTGAGATCCTGGGCAAAATCAACGGCGCCGTCGGAAACT
ATAACGCCCATCTGGCCGCCTACCCACAGATCGACTGGCACGCCTTCAGCGAAAGCTTTGTCACCTCGCTGGGGATCCAG
TGGAACCCCTACACCACCCAGATCGAACCGCACGACTATATCGCCGAGTTGTTTGACTGCGTCGCCCGCTTTAACACCAT
TCTGCTCGATTTCGACCGTGACGTATGGGGCTATATTGCGCTCAACCACTTCAAGCAGAGAACCATCGCTGGCGAAATCG
GCTCATCGACCATGCCACACAAGGTCAACCCGATCGACTTCGAAAACTCCGAAGGCAACCTGGGCCTCTCCAACGCCCTG
CTGGGACACCTGGCATCCAAGCTGCCGGTATCGCGCTGGCAGCGCGATCTGACCGACTCCACCGTCCTGCGCAACCTGGG
GGTCGGCCTCGGCTACGCCCTGATCGCCTATGCCTCGACTCTCAAGGGACTGAGCAAGCTGGAGGTCAACCAGGATCATC
TGCTGGACGAGCTGGATCACAACTGGGAGCTGCTGGCCGAGCCGGTGCAGACGGTGATGCGCCGCTACGGTATCGAAAAA
CCCTACGAAAAGCTCAAGGAGCTGACCCGTGGCAAACGGGTCGATGCTGCCGGGATGCAGCGCTTCATCGACGGCCTGGC
GCTGCCGGAAGAGGAGAAAGCGCGCCTGAAGGCGATGACCCCGGCCGCGTATACCGGCGCGGCGGCCGTGCTGGTCGATA
AATTAAAGTAG

Upstream 100 bases:

>100_bases
AGCCGGCTGCAGCTGATGTTCTCCCGTAATCGCCTATTTCAGATGGCGCAGAATTTATTGACCCACAGTTAACCTTTCCC
AATCCCAGGAGTTGCTACCG

Downstream 100 bases:

>100_bases
CACGCTGAACTGCACGAAATTACGCCACCCGTCTACGCGGGTGGCCTCTCCGTACTCCTCCACACGCCTGCGGTCACACT
GCGCTGACTTTTTACTTCTG

Product: adenylosuccinate lyase

Products: NA

Alternate protein names: ASL; Adenylosuccinase; ASase [H]

Number of amino acids: Translated: 456; Mature: 456

Protein sequence:

>456_residues
MELSSLTAVSPIDGRYGEKVSALRPIFSEFGLLKFRVQVEVRWLQKLAACAEIGEVPAFDADANAFLDRIADQFSEEDAR
RIKTIERTTNHDVKAVEYFLKEKVADQPALHAVSEFIHFACTSEDINNLSHALMLNSAREEVLKPVWRQLIDTLRDLAQR
YRDIPLMARTHGQPATPSTVGKEFANVAYRLERQFRQLTQVEILGKINGAVGNYNAHLAAYPQIDWHAFSESFVTSLGIQ
WNPYTTQIEPHDYIAELFDCVARFNTILLDFDRDVWGYIALNHFKQRTIAGEIGSSTMPHKVNPIDFENSEGNLGLSNAL
LGHLASKLPVSRWQRDLTDSTVLRNLGVGLGYALIAYASTLKGLSKLEVNQDHLLDELDHNWELLAEPVQTVMRRYGIEK
PYEKLKELTRGKRVDAAGMQRFIDGLALPEEEKARLKAMTPAAYTGAAAVLVDKLK

Sequences:

>Translated_456_residues
MELSSLTAVSPIDGRYGEKVSALRPIFSEFGLLKFRVQVEVRWLQKLAACAEIGEVPAFDADANAFLDRIADQFSEEDAR
RIKTIERTTNHDVKAVEYFLKEKVADQPALHAVSEFIHFACTSEDINNLSHALMLNSAREEVLKPVWRQLIDTLRDLAQR
YRDIPLMARTHGQPATPSTVGKEFANVAYRLERQFRQLTQVEILGKINGAVGNYNAHLAAYPQIDWHAFSESFVTSLGIQ
WNPYTTQIEPHDYIAELFDCVARFNTILLDFDRDVWGYIALNHFKQRTIAGEIGSSTMPHKVNPIDFENSEGNLGLSNAL
LGHLASKLPVSRWQRDLTDSTVLRNLGVGLGYALIAYASTLKGLSKLEVNQDHLLDELDHNWELLAEPVQTVMRRYGIEK
PYEKLKELTRGKRVDAAGMQRFIDGLALPEEEKARLKAMTPAAYTGAAAVLVDKLK
>Mature_456_residues
MELSSLTAVSPIDGRYGEKVSALRPIFSEFGLLKFRVQVEVRWLQKLAACAEIGEVPAFDADANAFLDRIADQFSEEDAR
RIKTIERTTNHDVKAVEYFLKEKVADQPALHAVSEFIHFACTSEDINNLSHALMLNSAREEVLKPVWRQLIDTLRDLAQR
YRDIPLMARTHGQPATPSTVGKEFANVAYRLERQFRQLTQVEILGKINGAVGNYNAHLAAYPQIDWHAFSESFVTSLGIQ
WNPYTTQIEPHDYIAELFDCVARFNTILLDFDRDVWGYIALNHFKQRTIAGEIGSSTMPHKVNPIDFENSEGNLGLSNAL
LGHLASKLPVSRWQRDLTDSTVLRNLGVGLGYALIAYASTLKGLSKLEVNQDHLLDELDHNWELLAEPVQTVMRRYGIEK
PYEKLKELTRGKRVDAAGMQRFIDGLALPEEEKARLKAMTPAAYTGAAAVLVDKLK

Specific function: De novo purine biosynthesis; eighth step. [C]

COG id: COG0015

COG function: function code F; Adenylosuccinate lyase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the lyase 1 family. Adenylosuccinate lyase subfamily [H]

Homologues:

Organism=Escherichia coli, GI1787376, Length=456, Percent_Identity=86.4035087719298, Blast_Score=832, Evalue=0.0,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013539
- InterPro:   IPR000362
- InterPro:   IPR020557
- InterPro:   IPR008948
- InterPro:   IPR022761
- InterPro:   IPR004769 [H]

Pfam domain/function: PF08328 ASL_C; PF00206 Lyase_1 [H]

EC number: =4.3.2.2 [H]

Molecular weight: Translated: 51365; Mature: 51365

Theoretical pI: Translated: 6.21; Mature: 6.21

Prosite motif: PS00163 FUMARATE_LYASES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MELSSLTAVSPIDGRYGEKVSALRPIFSEFGLLKFRVQVEVRWLQKLAACAEIGEVPAFD
CCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCEEEEEHHHHHHHHHHHHHHHHHCCCCCCC
ADANAFLDRIADQFSEEDARRIKTIERTTNHDVKAVEYFLKEKVADQPALHAVSEFIHFA
CCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHH
CTSEDINNLSHALMLNSAREEVLKPVWRQLIDTLRDLAQRYRDIPLMARTHGQPATPSTV
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHCCCCCCCCHHH
GKEFANVAYRLERQFRQLTQVEILGKINGAVGNYNAHLAAYPQIDWHAFSESFVTSLGIQ
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEECCCCHHHHHHHHHHHHCCCC
WNPYTTQIEPHDYIAELFDCVARFNTILLDFDRDVWGYIALNHFKQRTIAGEIGSSTMPH
CCCCCCCCCCHHHHHHHHHHHHHHHHEEEECCCCHHHHHHHHHHHHHHHHHHCCCCCCCC
KVNPIDFENSEGNLGLSNALLGHLASKLPVSRWQRDLTDSTVLRNLGVGLGYALIAYAST
CCCCCCCCCCCCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHH
LKGLSKLEVNQDHLLDELDHNWELLAEPVQTVMRRYGIEKPYEKLKELTRGKRVDAAGMQ
HHHHHHHCCCHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCCHHHHH
RFIDGLALPEEEKARLKAMTPAAYTGAAAVLVDKLK
HHHHHCCCCCHHHHHHHHCCCHHHHHHHHHHHHHCC
>Mature Secondary Structure
MELSSLTAVSPIDGRYGEKVSALRPIFSEFGLLKFRVQVEVRWLQKLAACAEIGEVPAFD
CCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCEEEEEHHHHHHHHHHHHHHHHHCCCCCCC
ADANAFLDRIADQFSEEDARRIKTIERTTNHDVKAVEYFLKEKVADQPALHAVSEFIHFA
CCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHH
CTSEDINNLSHALMLNSAREEVLKPVWRQLIDTLRDLAQRYRDIPLMARTHGQPATPSTV
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHCCCCCCCCHHH
GKEFANVAYRLERQFRQLTQVEILGKINGAVGNYNAHLAAYPQIDWHAFSESFVTSLGIQ
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEECCCCHHHHHHHHHHHHCCCC
WNPYTTQIEPHDYIAELFDCVARFNTILLDFDRDVWGYIALNHFKQRTIAGEIGSSTMPH
CCCCCCCCCCHHHHHHHHHHHHHHHHEEEECCCCHHHHHHHHHHHHHHHHHHCCCCCCCC
KVNPIDFENSEGNLGLSNALLGHLASKLPVSRWQRDLTDSTVLRNLGVGLGYALIAYAST
CCCCCCCCCCCCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHH
LKGLSKLEVNQDHLLDELDHNWELLAEPVQTVMRRYGIEKPYEKLKELTRGKRVDAAGMQ
HHHHHHHCCCHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCCHHHHH
RFIDGLALPEEEKARLKAMTPAAYTGAAAVLVDKLK
HHHHHCCCCCHHHHHHHHCCCHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12471157 [H]