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The map label for this gene is putP [H]

Identifier: 238920018

GI number: 238920018

Start: 2044587

End: 2045324

Strand: Reverse

Name: putP [H]

Synonym: NT01EI_2122

Alternate gene names: 238920018

Gene position: 2045324-2044587 (Counterclockwise)

Preceding gene: 238920019

Following gene: 238920015

Centisome position: 53.65

GC content: 63.55

Gene sequence:

>738_bases
ATGGCGGCCGACTCGCACCATAGCCTGATTAATGCCCGGCGTATCAGCATGACCTGGACGGTCTTCTGTCTGCTCGGTGC
CGTCTCCGTTGGTTTCTTCGGTATCGCCTACTTTACCAACCATCCTGCGCTGGCTGGTGCGGTGGATCGCAACGCTGAGC
AGGTCTTCATTGAGCTGGCGCAGCTGCTGTTTAACCCGTGGATCGCCGGTATTCTGCTGTCTGCCATCCTGGCGGCGGTG
ATGTCGACGCTCAGCTGCCAGCTGTTGGTGTGCTCCAGCGCGATTACCGAGGATCTCTATAGGGCCTTCCTGCGTCAGCA
GGCAGGACAGCGGGAACTGATGTGGGTTGGGCGGGCGATGGTGCTGCTGGTGGCGCTGGTTGCCATCGCGTTGGCCGCGA
ACCCGGAGAACCGGGTGCTGGGGCTGGTGAGCTATGCCTGGGCCGGTTTCGGGGCTGCCTTCGGCCCGGTGGTGATCTGC
TCGGTACTCTGGTCCCGTATGACCCGTAACGGTGCGCTGGCCGGGATGATCATCGGTGCCCTCACGGTGATTGTCTGGAA
ACAGTACGCCTGGCTGGGGCTGTATGAGATCATTCCGGGCTTCCTGTTCGCCGGCGTCGGTATCGTGGTGGTCAGTCTGC
TGGATCGCGAGCCCCCGGCGGCGGTGCGGCAGCGCTTCGCCGCCGCCGATGCCTGCTATCGTGCGTCACCGTGCGCCCCA
CAGCTGGAGTCGGAGTAA

Upstream 100 bases:

>100_bases
GACGTCGATATGCTGAAGGGGCTAAATCTGGTCGCTATCCTCTCGTTGCTGGGCTGGGGGCTGGGGCTACTTTGGCCAGC
CGCATATCCTGGCCCGTTTT

Downstream 100 bases:

>100_bases
CGGCGTCCGGGGGATGGCTGCGGTTCAGCGGAGGTATCGCCGCGGTATACCGGGACGATCCACCGTCATATCTAATGAGA
GAATAACGGTTGTACAATGA

Product: sodium/proline symporter

Products: NA

Alternate protein names: Proline permease; Propionate transporter [H]

Number of amino acids: Translated: 245; Mature: 244

Protein sequence:

>245_residues
MAADSHHSLINARRISMTWTVFCLLGAVSVGFFGIAYFTNHPALAGAVDRNAEQVFIELAQLLFNPWIAGILLSAILAAV
MSTLSCQLLVCSSAITEDLYRAFLRQQAGQRELMWVGRAMVLLVALVAIALAANPENRVLGLVSYAWAGFGAAFGPVVIC
SVLWSRMTRNGALAGMIIGALTVIVWKQYAWLGLYEIIPGFLFAGVGIVVVSLLDREPPAAVRQRFAAADACYRASPCAP
QLESE

Sequences:

>Translated_245_residues
MAADSHHSLINARRISMTWTVFCLLGAVSVGFFGIAYFTNHPALAGAVDRNAEQVFIELAQLLFNPWIAGILLSAILAAV
MSTLSCQLLVCSSAITEDLYRAFLRQQAGQRELMWVGRAMVLLVALVAIALAANPENRVLGLVSYAWAGFGAAFGPVVIC
SVLWSRMTRNGALAGMIIGALTVIVWKQYAWLGLYEIIPGFLFAGVGIVVVSLLDREPPAAVRQRFAAADACYRASPCAP
QLESE
>Mature_244_residues
AADSHHSLINARRISMTWTVFCLLGAVSVGFFGIAYFTNHPALAGAVDRNAEQVFIELAQLLFNPWIAGILLSAILAAVM
STLSCQLLVCSSAITEDLYRAFLRQQAGQRELMWVGRAMVLLVALVAIALAANPENRVLGLVSYAWAGFGAAFGPVVICS
VLWSRMTRNGALAGMIIGALTVIVWKQYAWLGLYEIIPGFLFAGVGIVVVSLLDREPPAAVRQRFAAADACYRASPCAPQ
LESE

Specific function: Catalyzes the sodium-dependent uptake of extracellular L-proline. This protein is also capable of using lithium as the transport cation. Also catalyzes the uptake of propionate [H]

COG id: COG0591

COG function: function code ER; Na+/proline symporter

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sodium:solute symporter (SSF) (TC 2.A.21) family [H]

Homologues:

Organism=Homo sapiens, GI310128183, Length=235, Percent_Identity=40.8510638297872, Blast_Score=176, Evalue=1e-44,
Organism=Escherichia coli, GI1787251, Length=244, Percent_Identity=79.9180327868852, Blast_Score=377, Evalue=1e-106,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011851
- InterPro:   IPR001734
- InterPro:   IPR018212
- InterPro:   IPR019900 [H]

Pfam domain/function: PF00474 SSF [H]

EC number: NA

Molecular weight: Translated: 26376; Mature: 26245

Theoretical pI: Translated: 7.79; Mature: 7.79

Prosite motif: PS00457 NA_SOLUT_SYMP_2 ; PS50283 NA_SOLUT_SYMP_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.4 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
5.3 %Cys+Met (Translated Protein)
2.5 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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CCCCC
>Mature Secondary Structure 
AADSHHSLINARRISMTWTVFCLLGAVSVGFFGIAYFTNHPALAGAVDRNAEQVFIELA
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHCCCHHHHHHHHH
QLLFNPWIAGILLSAILAAVMSTLSCQLLVCSSAITEDLYRAFLRQQAGQRELMWVGRAM
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHH
VLLVALVAIALAANPENRVLGLVSYAWAGFGAAFGPVVICSVLWSRMTRNGALAGMIIGA
HHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHH
LTVIVWKQYAWLGLYEIIPGFLFAGVGIVVVSLLDREPPAAVRQRFAAADACYRASPCAP
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCCCC
QLESE
CCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 3302614; 8905232; 9278503; 1400239; 3325781; 3053687; 3053649; 1567896; 8119910 [H]