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The map label for this gene is purR

Identifier: 238920006

GI number: 238920006

Start: 2029697

End: 2030722

Strand: Direct

Name: purR

Synonym: NT01EI_2110

Alternate gene names: 238920006

Gene position: 2029697-2030722 (Clockwise)

Preceding gene: 238920005

Following gene: 238920007

Centisome position: 53.24

GC content: 59.16

Gene sequence:

>1026_bases
ATGGCAACGATTAAAGATGTGGCAAAGCGCGCAGGCGTATCGACCACCACGGTGTCTCACGTCATCAATAAAACCCGCTT
TGTCGCTGACGAGACCAAAGCCGCCGTCTGGGAGGCCATTAAAGAACTACACTACTCCCCCAGCGCCGTTGCCCGCAGCC
TGAAGGTCAACCACACTAAATCTATCGGCCTGCTGGCGACCTCCAGCGAGGCACCATACTTTGCCGAGGTCATCGAATCG
GTAGAAAATAGCTGCTACGGCCATGGCTATACCCTGATCCTGTGTAACTCCCACAATAATCTGGAGAAACAGAAAGCCTA
TCTGGCCATGCTGGCACAAAAGCGTGTCGACGGCCTGCTGGTGATGTGCTCAGAGTATCCGGAAAACCTGTTGGAGAGCC
TGGAGGACTACCGCCACATCCCGATGGTGGTCATGGACTGGGGAACCCGCCGCAGCGACTTTACCGATGCGATCCAGGAC
AACGCCTTCGAGGGGGGCTATCTGGCCGGCCGCTATCTGATCGAGCGCGGGCACCGTGACATTGGCGCCATCACCGGCCC
GGTATCGCGTAACACCGGCGGCGGTCGCCTGCGCGGTTTCCTCAAGGCGCTGCAGGAGGCCAGCATCACGCCGCTGGAGT
CATGGTTGGTCGAAGGCGACTTCGAGCCGGAGTCTGGCTATACGGCAATGCAAAATATTCTGTCGCAGAAACAGCGCCCA
ACGGCGGTGTTTGTCGGCGGTGATATTATGGCGATGGGCGCCATTTGCGCAGCGGATGAGATGGGGCTGCGCGTACCGCA
GGATATCTCCGTCATCGGTTACGATAACGTGCGCAATGCCCGCTACTTTACCCCGGCGCTGACCACCATCCATCAGCCCA
AGGAGCGCCTTGGCGCCATGGCGTTCGACATGCTGCTCGACCGTATCACCAGTAAGCGCGAAGACGCCCAAACCATCGAA
GTTCACCCTAAGCTGGTTGAGCGCCGCTCCGTGGCCGACGGGCCGTTCATCGACTACCGCCGCTAA

Upstream 100 bases:

>100_bases
TCGGCGCCGAGCTGCGCTGTCAGGCGCATTTTCCGATGCACGCAGGCAAACGATTAACAAAACGGCGACGCAGCGCGTAC
GCCCAGGAGTCATTTGTTTC

Downstream 100 bases:

>100_bases
GCGTGTCACGACTGGCGCTGTTTCCCGCGCTGGTGCTGCTGTCCGGCATGCTGCCGCTGGCGGCGATGGTTCCGGCGACC
CTATTACCGCTGAGCGAACA

Product: DNA-binding transcriptional repressor PurR

Products: NA

Alternate protein names: Pur regulon repressor; Purine nucleotide synthesis repressor

Number of amino acids: Translated: 341; Mature: 340

Protein sequence:

>341_residues
MATIKDVAKRAGVSTTTVSHVINKTRFVADETKAAVWEAIKELHYSPSAVARSLKVNHTKSIGLLATSSEAPYFAEVIES
VENSCYGHGYTLILCNSHNNLEKQKAYLAMLAQKRVDGLLVMCSEYPENLLESLEDYRHIPMVVMDWGTRRSDFTDAIQD
NAFEGGYLAGRYLIERGHRDIGAITGPVSRNTGGGRLRGFLKALQEASITPLESWLVEGDFEPESGYTAMQNILSQKQRP
TAVFVGGDIMAMGAICAADEMGLRVPQDISVIGYDNVRNARYFTPALTTIHQPKERLGAMAFDMLLDRITSKREDAQTIE
VHPKLVERRSVADGPFIDYRR

Sequences:

>Translated_341_residues
MATIKDVAKRAGVSTTTVSHVINKTRFVADETKAAVWEAIKELHYSPSAVARSLKVNHTKSIGLLATSSEAPYFAEVIES
VENSCYGHGYTLILCNSHNNLEKQKAYLAMLAQKRVDGLLVMCSEYPENLLESLEDYRHIPMVVMDWGTRRSDFTDAIQD
NAFEGGYLAGRYLIERGHRDIGAITGPVSRNTGGGRLRGFLKALQEASITPLESWLVEGDFEPESGYTAMQNILSQKQRP
TAVFVGGDIMAMGAICAADEMGLRVPQDISVIGYDNVRNARYFTPALTTIHQPKERLGAMAFDMLLDRITSKREDAQTIE
VHPKLVERRSVADGPFIDYRR
>Mature_340_residues
ATIKDVAKRAGVSTTTVSHVINKTRFVADETKAAVWEAIKELHYSPSAVARSLKVNHTKSIGLLATSSEAPYFAEVIESV
ENSCYGHGYTLILCNSHNNLEKQKAYLAMLAQKRVDGLLVMCSEYPENLLESLEDYRHIPMVVMDWGTRRSDFTDAIQDN
AFEGGYLAGRYLIERGHRDIGAITGPVSRNTGGGRLRGFLKALQEASITPLESWLVEGDFEPESGYTAMQNILSQKQRPT
AVFVGGDIMAMGAICAADEMGLRVPQDISVIGYDNVRNARYFTPALTTIHQPKERLGAMAFDMLLDRITSKREDAQTIEV
HPKLVERRSVADGPFIDYRR

Specific function: Is the main repressor of the genes involved in the de novo synthesis of purine nucleotides, regulating purB, purC, purEK, purF, purHD, purL, purMN and guaBA expression. PurR is allosterically activated to bind its cognate DNA by binding the purine corepre

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lacI-type DNA-binding domain

Homologues:

Organism=Escherichia coli, GI1787948, Length=341, Percent_Identity=80.3519061583578, Blast_Score=584, Evalue=1e-168,
Organism=Escherichia coli, GI1790194, Length=330, Percent_Identity=48.1818181818182, Blast_Score=319, Evalue=2e-88,
Organism=Escherichia coli, GI1790369, Length=311, Percent_Identity=33.7620578778135, Blast_Score=199, Evalue=2e-52,
Organism=Escherichia coli, GI1788474, Length=333, Percent_Identity=31.2312312312312, Blast_Score=164, Evalue=8e-42,
Organism=Escherichia coli, GI1789202, Length=332, Percent_Identity=32.5301204819277, Blast_Score=158, Evalue=6e-40,
Organism=Escherichia coli, GI1787580, Length=324, Percent_Identity=29.9382716049383, Blast_Score=156, Evalue=1e-39,
Organism=Escherichia coli, GI1789068, Length=313, Percent_Identity=30.0319488817891, Blast_Score=143, Evalue=1e-35,
Organism=Escherichia coli, GI1786540, Length=311, Percent_Identity=33.1189710610932, Blast_Score=136, Evalue=2e-33,
Organism=Escherichia coli, GI48994940, Length=326, Percent_Identity=26.3803680981595, Blast_Score=107, Evalue=1e-24,
Organism=Escherichia coli, GI1789456, Length=338, Percent_Identity=27.810650887574, Blast_Score=101, Evalue=6e-23,
Organism=Escherichia coli, GI1790715, Length=328, Percent_Identity=23.4756097560976, Blast_Score=95, Evalue=5e-21,
Organism=Escherichia coli, GI1787906, Length=335, Percent_Identity=22.3880597014925, Blast_Score=87, Evalue=2e-18,
Organism=Escherichia coli, GI1786268, Length=280, Percent_Identity=25.3571428571429, Blast_Score=80, Evalue=2e-16,
Organism=Escherichia coli, GI1790192, Length=244, Percent_Identity=26.2295081967213, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI1790689, Length=318, Percent_Identity=22.3270440251572, Blast_Score=68, Evalue=1e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PURR_EDWI9 (C5BE45)

Other databases:

- EMBL:   CP001600
- RefSeq:   YP_002933521.1
- ProteinModelPortal:   C5BE45
- GeneID:   7959088
- GenomeReviews:   CP001600_GR
- KEGG:   eic:NT01EI_2110
- OMA:   ICAADEM
- ProtClustDB:   PRK10703
- GO:   GO:0005622
- HAMAP:   MF_01277
- InterPro:   IPR000843
- InterPro:   IPR010982
- InterPro:   IPR001761
- PRINTS:   PR00036
- SMART:   SM00354

Pfam domain/function: PF00356 LacI; PF00532 Peripla_BP_1; SSF47413 Lambda_like_DNA

EC number: NA

Molecular weight: Translated: 37865; Mature: 37733

Theoretical pI: Translated: 6.61; Mature: 6.61

Prosite motif: PS00356 HTH_LACI_1; PS50932 HTH_LACI_2

Important sites: BINDING 73-73 BINDING 190-190 BINDING 192-192 BINDING 221-221 BINDING 275-275

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MATIKDVAKRAGVSTTTVSHVINKTRFVADETKAAVWEAIKELHYSPSAVARSLKVNHTK
CCCHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCC
SIGLLATSSEAPYFAEVIESVENSCYGHGYTLILCNSHNNLEKQKAYLAMLAQKRVDGLL
CEEEEEECCCCCHHHHHHHHHHHCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHCCEE
VMCSEYPENLLESLEDYRHIPMVVMDWGTRRSDFTDAIQDNAFEGGYLAGRYLIERGHRD
EEHHHHHHHHHHHHHHHCCCCEEEEECCCCCCHHHHHHHCCCCCCCHHHHHHHHHHCCCH
IGAITGPVSRNTGGGRLRGFLKALQEASITPLESWLVEGDFEPESGYTAMQNILSQKQRP
HCCCCCCCCCCCCCHHHHHHHHHHHHCCCCHHHHHHHCCCCCCCCCHHHHHHHHHHCCCC
TAVFVGGDIMAMGAICAADEMGLRVPQDISVIGYDNVRNARYFTPALTTIHQPKERLGAM
CEEEECCHHHHHHHHHHHHHCCCCCCCCCEEEECCCCCCCEEECHHHHHHHCHHHHHHHH
AFDMLLDRITSKREDAQTIEVHPKLVERRSVADGPFIDYRR
HHHHHHHHHHCCCCCCCEEEECHHHHHHHCCCCCCCCCCCC
>Mature Secondary Structure 
ATIKDVAKRAGVSTTTVSHVINKTRFVADETKAAVWEAIKELHYSPSAVARSLKVNHTK
CCHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCC
SIGLLATSSEAPYFAEVIESVENSCYGHGYTLILCNSHNNLEKQKAYLAMLAQKRVDGLL
CEEEEEECCCCCHHHHHHHHHHHCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHCCEE
VMCSEYPENLLESLEDYRHIPMVVMDWGTRRSDFTDAIQDNAFEGGYLAGRYLIERGHRD
EEHHHHHHHHHHHHHHHCCCCEEEEECCCCCCHHHHHHHCCCCCCCHHHHHHHHHHCCCH
IGAITGPVSRNTGGGRLRGFLKALQEASITPLESWLVEGDFEPESGYTAMQNILSQKQRP
HCCCCCCCCCCCCCHHHHHHHHHHHHCCCCHHHHHHHCCCCCCCCCHHHHHHHHHHCCCC
TAVFVGGDIMAMGAICAADEMGLRVPQDISVIGYDNVRNARYFTPALTTIHQPKERLGAM
CEEEECCHHHHHHHHHHHHHCCCCCCCCCEEEECCCCCCCEEECHHHHHHHCHHHHHHHH
AFDMLLDRITSKREDAQTIEVHPKLVERRSVADGPFIDYRR
HHHHHHHHHHCCCCCCCEEEECHHHHHHHCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA