Click here to switch to the map view.

The map label for this gene is tyrS [H]

Identifier: 238919992

GI number: 238919992

Start: 2017907

End: 2019193

Strand: Reverse

Name: tyrS [H]

Synonym: NT01EI_2096

Alternate gene names: 238919992

Gene position: 2019193-2017907 (Counterclockwise)

Preceding gene: 238919993

Following gene: 238919991

Centisome position: 52.97

GC content: 57.19

Gene sequence:

>1287_bases
ATGGAGTTATTAATGGCAAGCAGCAACCTGATTAAACAACTGCAAGAGCGGGGCCTCGTTGCCCAGGTTACGGATGAAGA
AGCGTTGGCAGAGCGACTGGCGCAGGGGTCCATCGCACTGTATTGCGGATTCGATCCGACCGCTGACAGCTTGCATTTGG
GCCATCTGGTTCCCTTGCTGTGCCTTAAGCGCTTCCAGCAGGCCGGTCATAAGCCGGTGGCGCTGGTGGGTGGGGCCACC
GGTCTGATCGGCGATCCGAGCTTTAAGGCAACCGAGCGTAAGCTGAACACCGAAGAGACGGTGCAGGGCTGGGTGGACAA
AATCCGTCACCAGGTTGCGCCGTTCCTGGACTTCGACTGCGGGAGCAACAGCGCGATTGCTGCCAACAACTACGATTGGT
TTGGCGGCATGAACGTGCTGACCTTCCTGCGTGATATCGGCAAGCACTTCTCTGTTAACCAGATGATTAACAAAGAGGCG
GTTAAGCAGCGTCTGAACCGTGACGATGTGGGGATCTCCTTCACCGAGTTCTCCTACAACCTGCTGCAGGGCTATGACTT
TGCCAGCCTGAACCGGATGCACGGTGTCGAACTGCAGATCGGCGGATCTGATCAGTGGGGGAATATTACCTCCGGCATCG
ACCTGACCCGCCGCATGAACCAGCAGCAGGTGTGGGGTCTGACCGTCCCGCTGATCACCAAGGCCGATGGGACCAAGTTT
GGCAAGACCGAAGGCGGCGCGGTATGGCTGGATCCGAAAAAGACCAGCCCGTACAAGTTCTACCAGTTCTGGATCAATAC
CGCCGATGCCGACGTATACCGCTTCCTGAAGTTCTTCACCTTTATGGACATTGAGGAGATCAATGCACTGGAAGAGGAAG
ATAAGACCAGCGGCAAGGCACCGCGCGCACAGTATGTGCTGGCCGAGGCGGTGACGCGTCTGGTGCACGGCGAGGCGGGG
CTGGAAGCGGCTAAACGTATCACCCACAGCCTGTTCTCCGGCAGCCTGAGCGAGATGACCGAGGCTGACTTTGCCCAGCT
GGCGCAGGATGGTATGCCGATGATCGAAATGGATAAAGGCGCCGATTTGCAGCAGGCGTTAGTTGACGCTGAACTGCAGC
CATCGCGTGGCCAGGCGCGTAAAACCATCGCCTCTAACGCGGTCACCGTCAACGGTGAGAAACAGTCCGACCCTGAATAC
ACTTTCAGCGATGGCGATCGTCTGTTTAGCCGTTATACGCTGCTGCGTCGCGGCAAGAAGAACTATTGCCTGATCTGCTG
GAAGTAA

Upstream 100 bases:

>100_bases
CCTGTAAAGGCTGGCGTGGCGGAGCGTGGCGCTTTATCCTATGGCGTTTATTATCCGCCTTGACACCGCCCTGTAAGGGT
GACGGTGTGTGTTAACACTA

Downstream 100 bases:

>100_bases
TCACAGCAAGCAAGGGGGCTGCGGCCCCGGTTAATACCAACGGCTGCCCTGTGGGCAGATAAGCAAAAATACGATGAAGA
ATATCCTTTCCATTCAATCT

Product: tyrosyl-tRNA synthetase

Products: NA

Alternate protein names: Tyrosine--tRNA ligase; TyrRS [H]

Number of amino acids: Translated: 428; Mature: 428

Protein sequence:

>428_residues
MELLMASSNLIKQLQERGLVAQVTDEEALAERLAQGSIALYCGFDPTADSLHLGHLVPLLCLKRFQQAGHKPVALVGGAT
GLIGDPSFKATERKLNTEETVQGWVDKIRHQVAPFLDFDCGSNSAIAANNYDWFGGMNVLTFLRDIGKHFSVNQMINKEA
VKQRLNRDDVGISFTEFSYNLLQGYDFASLNRMHGVELQIGGSDQWGNITSGIDLTRRMNQQQVWGLTVPLITKADGTKF
GKTEGGAVWLDPKKTSPYKFYQFWINTADADVYRFLKFFTFMDIEEINALEEEDKTSGKAPRAQYVLAEAVTRLVHGEAG
LEAAKRITHSLFSGSLSEMTEADFAQLAQDGMPMIEMDKGADLQQALVDAELQPSRGQARKTIASNAVTVNGEKQSDPEY
TFSDGDRLFSRYTLLRRGKKNYCLICWK

Sequences:

>Translated_428_residues
MELLMASSNLIKQLQERGLVAQVTDEEALAERLAQGSIALYCGFDPTADSLHLGHLVPLLCLKRFQQAGHKPVALVGGAT
GLIGDPSFKATERKLNTEETVQGWVDKIRHQVAPFLDFDCGSNSAIAANNYDWFGGMNVLTFLRDIGKHFSVNQMINKEA
VKQRLNRDDVGISFTEFSYNLLQGYDFASLNRMHGVELQIGGSDQWGNITSGIDLTRRMNQQQVWGLTVPLITKADGTKF
GKTEGGAVWLDPKKTSPYKFYQFWINTADADVYRFLKFFTFMDIEEINALEEEDKTSGKAPRAQYVLAEAVTRLVHGEAG
LEAAKRITHSLFSGSLSEMTEADFAQLAQDGMPMIEMDKGADLQQALVDAELQPSRGQARKTIASNAVTVNGEKQSDPEY
TFSDGDRLFSRYTLLRRGKKNYCLICWK
>Mature_428_residues
MELLMASSNLIKQLQERGLVAQVTDEEALAERLAQGSIALYCGFDPTADSLHLGHLVPLLCLKRFQQAGHKPVALVGGAT
GLIGDPSFKATERKLNTEETVQGWVDKIRHQVAPFLDFDCGSNSAIAANNYDWFGGMNVLTFLRDIGKHFSVNQMINKEA
VKQRLNRDDVGISFTEFSYNLLQGYDFASLNRMHGVELQIGGSDQWGNITSGIDLTRRMNQQQVWGLTVPLITKADGTKF
GKTEGGAVWLDPKKTSPYKFYQFWINTADADVYRFLKFFTFMDIEEINALEEEDKTSGKAPRAQYVLAEAVTRLVHGEAG
LEAAKRITHSLFSGSLSEMTEADFAQLAQDGMPMIEMDKGADLQQALVDAELQPSRGQARKTIASNAVTVNGEKQSDPEY
TFSDGDRLFSRYTLLRRGKKNYCLICWK

Specific function: Catalyzes the attachment of tyrosine to tRNA(Tyr) in a two-step reaction:tyrosine is first activated by ATP to form Tyr- AMP and then transferred to the acceptor end of tRNA(Tyr) [H]

COG id: COG0162

COG function: function code J; Tyrosyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S4 RNA-binding domain [H]

Homologues:

Organism=Homo sapiens, GI94681057, Length=403, Percent_Identity=36.7245657568238, Blast_Score=245, Evalue=5e-65,
Organism=Escherichia coli, GI1787925, Length=424, Percent_Identity=90.3301886792453, Blast_Score=803, Evalue=0.0,
Organism=Caenorhabditis elegans, GI25145802, Length=407, Percent_Identity=36.3636363636364, Blast_Score=234, Evalue=9e-62,
Organism=Caenorhabditis elegans, GI25145805, Length=330, Percent_Identity=34.2424242424242, Blast_Score=174, Evalue=9e-44,
Organism=Saccharomyces cerevisiae, GI6325160, Length=438, Percent_Identity=35.8447488584475, Blast_Score=271, Evalue=1e-73,
Organism=Drosophila melanogaster, GI20130383, Length=445, Percent_Identity=36.4044943820225, Blast_Score=296, Evalue=1e-80,

Paralogues:

None

Copy number: 892 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001412
- InterPro:   IPR002305
- InterPro:   IPR014729
- InterPro:   IPR002942
- InterPro:   IPR002307 [H]

Pfam domain/function: PF01479 S4; PF00579 tRNA-synt_1b [H]

EC number: =6.1.1.1 [H]

Molecular weight: Translated: 47679; Mature: 47679

Theoretical pI: Translated: 5.70; Mature: 5.70

Prosite motif: PS50889 S4 ; PS00178 AA_TRNA_LIGASE_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MELLMASSNLIKQLQERGLVAQVTDEEALAERLAQGSIALYCGFDPTADSLHLGHLVPLL
CCEEEHHHHHHHHHHHCCCEEEECCHHHHHHHHHCCCEEEEECCCCCCCCCHHHHHHHHH
CLKRFQQAGHKPVALVGGATGLIGDPSFKATERKLNTEETVQGWVDKIRHQVAPFLDFDC
HHHHHHHCCCCCEEEECCCCCCCCCCCCHHHHHHCCHHHHHHHHHHHHHHHHCCCEECCC
GSNSAIAANNYDWFGGMNVLTFLRDIGKHFSVNQMINKEAVKQRLNRDDVGISFTEFSYN
CCCCEEEECCCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCCEEEHHHHHH
LLQGYDFASLNRMHGVELQIGGSDQWGNITSGIDLTRRMNQQQVWGLTVPLITKADGTKF
HHHCCCHHHHHCCCCEEEEECCCCCCCCCCCCHHHHHHCCCHHHCEEEEEEEECCCCCCC
GKTEGGAVWLDPKKTSPYKFYQFWINTADADVYRFLKFFTFMDIEEINALEEEDKTSGKA
CCCCCCEEEECCCCCCCCEEEEEEECCCHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCC
PRAQYVLAEAVTRLVHGEAGLEAAKRITHSLFSGSLSEMTEADFAQLAQDGMPMIEMDKG
CHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCEEEECCC
ADLQQALVDAELQPSRGQARKTIASNAVTVNGEKQSDPEYTFSDGDRLFSRYTLLRRGKK
CCHHHHHHCCCCCCCCCHHHHHHHCCCEEECCCCCCCCCCCCCCHHHHHHHHHHHHCCCC
NYCLICWK
CEEEEEEC
>Mature Secondary Structure
MELLMASSNLIKQLQERGLVAQVTDEEALAERLAQGSIALYCGFDPTADSLHLGHLVPLL
CCEEEHHHHHHHHHHHCCCEEEECCHHHHHHHHHCCCEEEEECCCCCCCCCHHHHHHHHH
CLKRFQQAGHKPVALVGGATGLIGDPSFKATERKLNTEETVQGWVDKIRHQVAPFLDFDC
HHHHHHHCCCCCEEEECCCCCCCCCCCCHHHHHHCCHHHHHHHHHHHHHHHHCCCEECCC
GSNSAIAANNYDWFGGMNVLTFLRDIGKHFSVNQMINKEAVKQRLNRDDVGISFTEFSYN
CCCCEEEECCCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCCEEEHHHHHH
LLQGYDFASLNRMHGVELQIGGSDQWGNITSGIDLTRRMNQQQVWGLTVPLITKADGTKF
HHHCCCHHHHHCCCCEEEEECCCCCCCCCCCCHHHHHHCCCHHHCEEEEEEEECCCCCCC
GKTEGGAVWLDPKKTSPYKFYQFWINTADADVYRFLKFFTFMDIEEINALEEEDKTSGKA
CCCCCCEEEECCCCCCCCEEEEEEECCCHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCC
PRAQYVLAEAVTRLVHGEAGLEAAKRITHSLFSGSLSEMTEADFAQLAQDGMPMIEMDKG
CHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCEEEECCC
ADLQQALVDAELQPSRGQARKTIASNAVTVNGEKQSDPEYTFSDGDRLFSRYTLLRRGKK
CCHHHHHHCCCCCCCCCHHHHHHHCCCEEECCCCCCCCCCCCCCHHHHHHHHHHHHCCCC
NYCLICWK
CEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA