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The map label for this gene is rstB [H]

Identifier: 238919846

GI number: 238919846

Start: 1879941

End: 1881251

Strand: Reverse

Name: rstB [H]

Synonym: NT01EI_1950

Alternate gene names: 238919846

Gene position: 1881251-1879941 (Counterclockwise)

Preceding gene: 238919847

Following gene: 238919845

Centisome position: 49.35

GC content: 57.36

Gene sequence:

>1311_bases
ATGCGCAAACTGTTCATTCAGTTCTACCTGCTGCTGTTTGTCTGCTTCCTGGTTATGGCCATGCTGGTCGGTCTGGTTTA
TAAGGTCACCGCCGAGCGCGCTGGTCGCCAGTCGATGGATGATCTGATGAAAAGCTCGCTGTATCTGATCCGCAGCCAGC
TGCGTGAGATCCCACCGCGCGATTGGAATAAGACCATCGACAAACTCGACTGGAATCTGTCGTTTCAGTTTCATATCGAG
CCTCTCGGCAAGCGCCACCTCTCGCCCGGACAGGATCGTAAATTGCGTGAGGGCGAGATCGTCGCATTGGACAGCGAATA
TATTTTTTTACAGCGTATTCCGCGCAGCCACTATGTCCTGGCGGTCGGACCAATCCCCTACCTGTTTTTTCTGCATGAGA
TGCGCCTGTTTGATTTTGCCCTGATGCTGCTTATCGGACTCTCGCTGGCGCTGCCGGTATTTCTGTGGATGCGCCCCCAT
TGGCAGGATCTGCTCAGGCTGGAAAACGCTGCCCAGCGCCTGGGCGAGGGACATCTGCAGGAGCGAACGCACTTTGACAG
CGCCTCCAGCCTGCATCGCTTAGGGGTCGCGTTTAACCGTATGGCTGACAATATCAATACCCTTATCGCCAGCAAGAAGC
AGCTGATTGACGGTATTGCCCATGAGTTGCGGACGCCGCTGGTACGTCTGCGTTACCGGTTGGCTATGAGCGACAGTTTA
AGCGCCGGGGAACAGGCGGCGATCAATCGTGATATCGGTCAGTTGGAGGGATTGATCGATGAGCTGCTGACCTATGCACG
TCTTGATCGCCCTCAGGTAAGCCCCCATCTGGAGGCGATCGACCTGCCCGCCTGGCTGGAGGATAAAGCCCACGAACTGC
GTCAGATGCACCCGCAGCAGCAAATCTCGCTGGATATCCCTCATCGGGGCGATTTCGGTGCTCTCGATATCCGTCTGTTC
GAGCGCCTGCTGGATAACCTGATCAACAATGCCCTACGCTATAATCAGGACAAGCTGCGTATCGGACTGTGGTTTGACGG
CGATAATGGCTGTCTCCAGGTGGAGGATGACGGGCCTGGCATTCCCACGGCAGAGCGCGACAAGGTCTTTGAGCCCTTTG
TCCGGCTGGATCCCAGCCGCGATCGCGCCACCGGCGGCTGTGGCCTGGGACTGGCCATCGTCTACAGTATCGTGCAGGCC
TATCAGGGGCAGATTGCGATTAGCAGTTCGTCGCTGGGCGGCGCCTGTTTCCGGTTTACCTGGCCGCGTCATAGCACACC
GCTCCCATCGGTGCCCTCCGCATCCGCCTGA

Upstream 100 bases:

>100_bases
TGCGCCGCAAGCTGCACGATACCGGGCCAGAGCCCGTGCGCATCAAAACCGTACGCAATAAGGGATACCTGTTTGCACCT
AACGCCTGGGAATAGTACCG

Downstream 100 bases:

>100_bases
GTCCAATCACCCTACAGACGGCCCCATGGGCCGTGGAGTCACTATCATGATCAGTGCCTATCAACGTTTGGAAACGATCT
TTCAGCGCCTGGCGCGCTTT

Product: sensor protein RstB

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 436; Mature: 436

Protein sequence:

>436_residues
MRKLFIQFYLLLFVCFLVMAMLVGLVYKVTAERAGRQSMDDLMKSSLYLIRSQLREIPPRDWNKTIDKLDWNLSFQFHIE
PLGKRHLSPGQDRKLREGEIVALDSEYIFLQRIPRSHYVLAVGPIPYLFFLHEMRLFDFALMLLIGLSLALPVFLWMRPH
WQDLLRLENAAQRLGEGHLQERTHFDSASSLHRLGVAFNRMADNINTLIASKKQLIDGIAHELRTPLVRLRYRLAMSDSL
SAGEQAAINRDIGQLEGLIDELLTYARLDRPQVSPHLEAIDLPAWLEDKAHELRQMHPQQQISLDIPHRGDFGALDIRLF
ERLLDNLINNALRYNQDKLRIGLWFDGDNGCLQVEDDGPGIPTAERDKVFEPFVRLDPSRDRATGGCGLGLAIVYSIVQA
YQGQIAISSSSLGGACFRFTWPRHSTPLPSVPSASA

Sequences:

>Translated_436_residues
MRKLFIQFYLLLFVCFLVMAMLVGLVYKVTAERAGRQSMDDLMKSSLYLIRSQLREIPPRDWNKTIDKLDWNLSFQFHIE
PLGKRHLSPGQDRKLREGEIVALDSEYIFLQRIPRSHYVLAVGPIPYLFFLHEMRLFDFALMLLIGLSLALPVFLWMRPH
WQDLLRLENAAQRLGEGHLQERTHFDSASSLHRLGVAFNRMADNINTLIASKKQLIDGIAHELRTPLVRLRYRLAMSDSL
SAGEQAAINRDIGQLEGLIDELLTYARLDRPQVSPHLEAIDLPAWLEDKAHELRQMHPQQQISLDIPHRGDFGALDIRLF
ERLLDNLINNALRYNQDKLRIGLWFDGDNGCLQVEDDGPGIPTAERDKVFEPFVRLDPSRDRATGGCGLGLAIVYSIVQA
YQGQIAISSSSLGGACFRFTWPRHSTPLPSVPSASA
>Mature_436_residues
MRKLFIQFYLLLFVCFLVMAMLVGLVYKVTAERAGRQSMDDLMKSSLYLIRSQLREIPPRDWNKTIDKLDWNLSFQFHIE
PLGKRHLSPGQDRKLREGEIVALDSEYIFLQRIPRSHYVLAVGPIPYLFFLHEMRLFDFALMLLIGLSLALPVFLWMRPH
WQDLLRLENAAQRLGEGHLQERTHFDSASSLHRLGVAFNRMADNINTLIASKKQLIDGIAHELRTPLVRLRYRLAMSDSL
SAGEQAAINRDIGQLEGLIDELLTYARLDRPQVSPHLEAIDLPAWLEDKAHELRQMHPQQQISLDIPHRGDFGALDIRLF
ERLLDNLINNALRYNQDKLRIGLWFDGDNGCLQVEDDGPGIPTAERDKVFEPFVRLDPSRDRATGGCGLGLAIVYSIVQA
YQGQIAISSSSLGGACFRFTWPRHSTPLPSVPSASA

Specific function: Member of the two-component regulatory system rstB/rstA. RstB functions as a membrane-associated protein kinase that phosphorylates rstA (Potential) [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 histidine kinase domain [H]

Homologues:

Organism=Escherichia coli, GI1787894, Length=428, Percent_Identity=65.6542056074766, Blast_Score=580, Evalue=1e-167,
Organism=Escherichia coli, GI1790346, Length=260, Percent_Identity=33.4615384615385, Blast_Score=139, Evalue=5e-34,
Organism=Escherichia coli, GI1789808, Length=291, Percent_Identity=30.9278350515464, Blast_Score=121, Evalue=1e-28,
Organism=Escherichia coli, GI1788393, Length=266, Percent_Identity=30.8270676691729, Blast_Score=103, Evalue=3e-23,
Organism=Escherichia coli, GI1786912, Length=255, Percent_Identity=30.9803921568627, Blast_Score=99, Evalue=4e-22,
Organism=Escherichia coli, GI1786783, Length=251, Percent_Identity=30.2788844621514, Blast_Score=99, Evalue=7e-22,
Organism=Escherichia coli, GI1786600, Length=242, Percent_Identity=28.5123966942149, Blast_Score=89, Evalue=6e-19,
Organism=Escherichia coli, GI87082128, Length=303, Percent_Identity=27.3927392739274, Blast_Score=86, Evalue=4e-18,
Organism=Escherichia coli, GI1788279, Length=279, Percent_Identity=29.0322580645161, Blast_Score=85, Evalue=1e-17,
Organism=Escherichia coli, GI1790551, Length=217, Percent_Identity=30.4147465437788, Blast_Score=81, Evalue=1e-16,
Organism=Escherichia coli, GI1787374, Length=334, Percent_Identity=26.3473053892216, Blast_Score=76, Evalue=5e-15,
Organism=Escherichia coli, GI1788549, Length=224, Percent_Identity=26.3392857142857, Blast_Score=74, Evalue=2e-14,
Organism=Escherichia coli, GI48994928, Length=237, Percent_Identity=26.1603375527426, Blast_Score=74, Evalue=2e-14,
Organism=Escherichia coli, GI145693157, Length=260, Percent_Identity=25.3846153846154, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI1788713, Length=281, Percent_Identity=24.9110320284698, Blast_Score=70, Evalue=3e-13,
Organism=Escherichia coli, GI87081816, Length=119, Percent_Identity=33.6134453781513, Blast_Score=68, Evalue=1e-12,
Organism=Escherichia coli, GI1789149, Length=229, Percent_Identity=26.6375545851528, Blast_Score=67, Evalue=2e-12,
Organism=Escherichia coli, GI1790861, Length=222, Percent_Identity=26.5765765765766, Blast_Score=65, Evalue=7e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR003660
- InterPro:   IPR004358
- InterPro:   IPR003661
- InterPro:   IPR005467
- InterPro:   IPR009082 [H]

Pfam domain/function: PF00672 HAMP; PF02518 HATPase_c; PF00512 HisKA [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 49742; Mature: 49742

Theoretical pI: Translated: 7.22; Mature: 7.22

Prosite motif: PS50885 HAMP ; PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRKLFIQFYLLLFVCFLVMAMLVGLVYKVTAERAGRQSMDDLMKSSLYLIRSQLREIPPR
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCC
DWNKTIDKLDWNLSFQFHIEPLGKRHLSPGQDRKLREGEIVALDSEYIFLQRIPRSHYVL
CHHHHHHHCCCCEEEEEEECCCCCCCCCCCCCCCCCCCCEEEECCCEEEEEECCCCCEEE
AVGPIPYLFFLHEMRLFDFALMLLIGLSLALPVFLWMRPHWQDLLRLENAAQRLGEGHLQ
EECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCHHH
ERTHFDSASSLHRLGVAFNRMADNINTLIASKKQLIDGIAHELRTPLVRLRYRLAMSDSL
HHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
SAGEQAAINRDIGQLEGLIDELLTYARLDRPQVSPHLEAIDLPAWLEDKAHELRQMHPQQ
CCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHCCCCHHHHHHHHHHHHHCCCH
QISLDIPHRGDFGALDIRLFERLLDNLINNALRYNQDKLRIGLWFDGDNGCLQVEDDGPG
HEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEEECCCCEEEECCCCCC
IPTAERDKVFEPFVRLDPSRDRATGGCGLGLAIVYSIVQAYQGQIAISSSSLGGACFRFT
CCCCCHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHCCCEEEECCCCCCEEEEEE
WPRHSTPLPSVPSASA
CCCCCCCCCCCCCCCC
>Mature Secondary Structure
MRKLFIQFYLLLFVCFLVMAMLVGLVYKVTAERAGRQSMDDLMKSSLYLIRSQLREIPPR
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCC
DWNKTIDKLDWNLSFQFHIEPLGKRHLSPGQDRKLREGEIVALDSEYIFLQRIPRSHYVL
CHHHHHHHCCCCEEEEEEECCCCCCCCCCCCCCCCCCCCEEEECCCEEEEEECCCCCEEE
AVGPIPYLFFLHEMRLFDFALMLLIGLSLALPVFLWMRPHWQDLLRLENAAQRLGEGHLQ
EECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCHHH
ERTHFDSASSLHRLGVAFNRMADNINTLIASKKQLIDGIAHELRTPLVRLRYRLAMSDSL
HHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
SAGEQAAINRDIGQLEGLIDELLTYARLDRPQVSPHLEAIDLPAWLEDKAHELRQMHPQQ
CCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHCCCCHHHHHHHHHHHHHCCCH
QISLDIPHRGDFGALDIRLFERLLDNLINNALRYNQDKLRIGLWFDGDNGCLQVEDDGPG
HEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEEECCCCEEEECCCCCC
IPTAERDKVFEPFVRLDPSRDRATGGCGLGLAIVYSIVQAYQGQIAISSSSLGGACFRFT
CCCCCHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHCCCEEEECCCCCCEEEEEE
WPRHSTPLPSVPSASA
CCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9097039; 9278503; 2646639; 1925016; 1495392 [H]