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The map label for this gene is 238919845

Identifier: 238919845

GI number: 238919845

Start: 1878389

End: 1879894

Strand: Reverse

Name: 238919845

Synonym: NT01EI_1949

Alternate gene names: NA

Gene position: 1879894-1878389 (Counterclockwise)

Preceding gene: 238919846

Following gene: 238919843

Centisome position: 49.31

GC content: 60.16

Gene sequence:

>1506_bases
ATGATCAGTGCCTATCAACGTTTGGAAACGATCTTTCAGCGCCTGGCGCGCTTTGACCATCTCTCGGCCATCGCCGGCTG
GGATATGCAGACCTACATGCCGAGCGGCGGCAGCGGCGCCCGCTCAGAGGCACTTACCGAGCTTAACCTGCTGATGCACC
GGATCTTGTGCGACGAAGAGACCGGCGTCTGGCTACAGCGCGCCGCGCAGGAGAGCCTCAGCGATGAGCAGCAGGCTAAT
TTAAGCGAAATGCAGCGTCGTTATGATCGTGCCAGCCTGCTCCCCGCGGCCTTGGTCGAGGAGAAATCTCTGGCCGGAAT
GCGCTGCGAGCATGCCTGGCGGACGCAACGTGCCAATAATGACTGGCACGGATTTATGGAGAATCTGCGCCCGGTCGTCA
GGCTGAGTCGGGAGGAGGCCAAAATCCGCGCAGATCACGCCGGATGTAGCCGCTATGATGCCCTGTTGGATCTATATGAA
CCGGGAATGAACAGCGCGCGCCTCGATGCCCTGTTCGGCGAGATTAAAGGCTGGCTGCCTACGCTGCTACAACGGGTGGT
AGAGCAACAGAAATGCACGCCGCCGTTATTGCCCCGCGGGCCATTCGATGAGGAGGCGCAGCGTCGGCTTGGGCTGCGTA
TCATGACGCAGCTGGGCTTCGACTTTGCCCACGGCCGTCTGGACGTCAGCGCCCACCCCTTTTGTGGCGGCGTTCCTCAA
GATGTCCGTATCACCACCCGCTATAGCCACGATGACTTTCTTAGCGCGCTGCTGGGCGTCGTGCATGAAACCGGCCACGC
CCGCTATGAACAAAATCTACCGCATGCCTGGGCGGGACAACCGCTGGGCGAAGCGCGATCCACCGCCATCCATGAGTCCC
AGAGCCTGTTTTTTGAGATGCAGCTGGCGCGCAGTCACGGCTTCCTGAACTTTCTGCGCCCGTGGGTTATCGAGGCCTTC
GGCGACGATCCGGCCTTCAGCGCCGAAAACTTTGTCCGCCTCAATCAGCGCGTCAGGCCGGGCTATATCCGGGTCGATGC
CGATGAAGTCAGCTATCCGGCGCACATCATTTTACGCTATGAGATCGAACGGGCGCTGATTGAGGGCGAGATTGAGGTAG
AGGATATTCCGGCGCTGTGGGACAGCCAGATGCACCGCACGCTGGGACTGAGCACCGACGGCAATTACCGCGATGGATGC
ATGCAGGATATTCACTGGACTGACGGTGCCTTTGGCTACTTCCCCACCTATACCCTCGGCGCAATGTATGCCGCGCAGTT
GTACGCCGCCGCCCGCCGGGATATCGCCGATCTCCCGGCGCAGATCGCCGCCGGTGATATCGCACCGATAGGTCACTGGC
TGCGTCAACATATCTGGAGCCATGGTTCACGCTGGGATACCGATACCCTAATCCGTAACGTCTGCGGTGAACCGCTGAAC
CCAGATTACCTGCGGCGCCATCTGGAGCAGCGTTATCTTGGCTCCATGAGAGACAGCCTGCGCTAA

Upstream 100 bases:

>100_bases
ACCTGGCCGCGTCATAGCACACCGCTCCCATCGGTGCCCTCCGCATCCGCCTGAGTCCAATCACCCTACAGACGGCCCCA
TGGGCCGTGGAGTCACTATC

Downstream 100 bases:

>100_bases
GGCATTTTAAGTTTAAGACCTGTTTGTATCGGATAATGACGCGTCTCCGGCGCGTTATTATCGTTGTGATTCAGCGTTGC
TGTGTTGGAGAGATGCCTAT

Product: thermostable carboxypeptidase 1

Products: NA

Alternate protein names: Carboxypeptidase Taq [H]

Number of amino acids: Translated: 501; Mature: 501

Protein sequence:

>501_residues
MISAYQRLETIFQRLARFDHLSAIAGWDMQTYMPSGGSGARSEALTELNLLMHRILCDEETGVWLQRAAQESLSDEQQAN
LSEMQRRYDRASLLPAALVEEKSLAGMRCEHAWRTQRANNDWHGFMENLRPVVRLSREEAKIRADHAGCSRYDALLDLYE
PGMNSARLDALFGEIKGWLPTLLQRVVEQQKCTPPLLPRGPFDEEAQRRLGLRIMTQLGFDFAHGRLDVSAHPFCGGVPQ
DVRITTRYSHDDFLSALLGVVHETGHARYEQNLPHAWAGQPLGEARSTAIHESQSLFFEMQLARSHGFLNFLRPWVIEAF
GDDPAFSAENFVRLNQRVRPGYIRVDADEVSYPAHIILRYEIERALIEGEIEVEDIPALWDSQMHRTLGLSTDGNYRDGC
MQDIHWTDGAFGYFPTYTLGAMYAAQLYAAARRDIADLPAQIAAGDIAPIGHWLRQHIWSHGSRWDTDTLIRNVCGEPLN
PDYLRRHLEQRYLGSMRDSLR

Sequences:

>Translated_501_residues
MISAYQRLETIFQRLARFDHLSAIAGWDMQTYMPSGGSGARSEALTELNLLMHRILCDEETGVWLQRAAQESLSDEQQAN
LSEMQRRYDRASLLPAALVEEKSLAGMRCEHAWRTQRANNDWHGFMENLRPVVRLSREEAKIRADHAGCSRYDALLDLYE
PGMNSARLDALFGEIKGWLPTLLQRVVEQQKCTPPLLPRGPFDEEAQRRLGLRIMTQLGFDFAHGRLDVSAHPFCGGVPQ
DVRITTRYSHDDFLSALLGVVHETGHARYEQNLPHAWAGQPLGEARSTAIHESQSLFFEMQLARSHGFLNFLRPWVIEAF
GDDPAFSAENFVRLNQRVRPGYIRVDADEVSYPAHIILRYEIERALIEGEIEVEDIPALWDSQMHRTLGLSTDGNYRDGC
MQDIHWTDGAFGYFPTYTLGAMYAAQLYAAARRDIADLPAQIAAGDIAPIGHWLRQHIWSHGSRWDTDTLIRNVCGEPLN
PDYLRRHLEQRYLGSMRDSLR
>Mature_501_residues
MISAYQRLETIFQRLARFDHLSAIAGWDMQTYMPSGGSGARSEALTELNLLMHRILCDEETGVWLQRAAQESLSDEQQAN
LSEMQRRYDRASLLPAALVEEKSLAGMRCEHAWRTQRANNDWHGFMENLRPVVRLSREEAKIRADHAGCSRYDALLDLYE
PGMNSARLDALFGEIKGWLPTLLQRVVEQQKCTPPLLPRGPFDEEAQRRLGLRIMTQLGFDFAHGRLDVSAHPFCGGVPQ
DVRITTRYSHDDFLSALLGVVHETGHARYEQNLPHAWAGQPLGEARSTAIHESQSLFFEMQLARSHGFLNFLRPWVIEAF
GDDPAFSAENFVRLNQRVRPGYIRVDADEVSYPAHIILRYEIERALIEGEIEVEDIPALWDSQMHRTLGLSTDGNYRDGC
MQDIHWTDGAFGYFPTYTLGAMYAAQLYAAARRDIADLPAQIAAGDIAPIGHWLRQHIWSHGSRWDTDTLIRNVCGEPLN
PDYLRRHLEQRYLGSMRDSLR

Specific function: Releases amino acids sequentially from the C-terminus with a broad substrate specificity (except for proline) [H]

COG id: COG2317

COG function: function code E; Zn-dependent carboxypeptidase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase M32 family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001333 [H]

Pfam domain/function: PF02074 Peptidase_M32 [H]

EC number: =3.4.17.19 [H]

Molecular weight: Translated: 57157; Mature: 57157

Theoretical pI: Translated: 5.99; Mature: 5.99

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MISAYQRLETIFQRLARFDHLSAIAGWDMQTYMPSGGSGARSEALTELNLLMHRILCDEE
CCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHCCCCCCCCHHHHHHHHHHHHHHHHHCCCC
TGVWLQRAAQESLSDEQQANLSEMQRRYDRASLLPAALVEEKSLAGMRCEHAWRTQRANN
CCHHHHHHHHHHCCHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCC
DWHGFMENLRPVVRLSREEAKIRADHAGCSRYDALLDLYEPGMNSARLDALFGEIKGWLP
HHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHH
TLLQRVVEQQKCTPPLLPRGPFDEEAQRRLGLRIMTQLGFDFAHGRLDVSAHPFCGGVPQ
HHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCHHHCCEECCCCCCCCCCCCC
DVRITTRYSHDDFLSALLGVVHETGHARYEQNLPHAWAGQPLGEARSTAIHESQSLFFEM
CCEEEEECCHHHHHHHHHHHHHHHCCHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHH
QLARSHGFLNFLRPWVIEAFGDDPAFSAENFVRLNQRVRPGYIRVDADEVSYPAHIILRY
HHHHHCCHHHHHHHHHHHHCCCCCCCCHHHHHHHHCCCCCCEEEECHHHCCCCEEEEEEE
EIERALIEGEIEVEDIPALWDSQMHRTLGLSTDGNYRDGCMQDIHWTDGAFGYFPTYTLG
HHHHHHHCCCCCCHHCCHHHHHHHHHHHCCCCCCCCCCCHHCCCCCCCCCCCCCHHHHHH
AMYAAQLYAAARRDIADLPAQIAAGDIAPIGHWLRQHIWSHGSRWDTDTLIRNVCGEPLN
HHHHHHHHHHHHHHHHHCCHHHHCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHCCCCCC
PDYLRRHLEQRYLGSMRDSLR
HHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MISAYQRLETIFQRLARFDHLSAIAGWDMQTYMPSGGSGARSEALTELNLLMHRILCDEE
CCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHCCCCCCCCHHHHHHHHHHHHHHHHHCCCC
TGVWLQRAAQESLSDEQQANLSEMQRRYDRASLLPAALVEEKSLAGMRCEHAWRTQRANN
CCHHHHHHHHHHCCHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCC
DWHGFMENLRPVVRLSREEAKIRADHAGCSRYDALLDLYEPGMNSARLDALFGEIKGWLP
HHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHH
TLLQRVVEQQKCTPPLLPRGPFDEEAQRRLGLRIMTQLGFDFAHGRLDVSAHPFCGGVPQ
HHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCHHHCCEECCCCCCCCCCCCC
DVRITTRYSHDDFLSALLGVVHETGHARYEQNLPHAWAGQPLGEARSTAIHESQSLFFEM
CCEEEEECCHHHHHHHHHHHHHHHCCHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHH
QLARSHGFLNFLRPWVIEAFGDDPAFSAENFVRLNQRVRPGYIRVDADEVSYPAHIILRY
HHHHHCCHHHHHHHHHHHHCCCCCCCCHHHHHHHHCCCCCCEEEECHHHCCCCEEEEEEE
EIERALIEGEIEVEDIPALWDSQMHRTLGLSTDGNYRDGCMQDIHWTDGAFGYFPTYTLG
HHHHHHHCCCCCCHHCCHHHHHHHHHHHCCCCCCCCCCCHHCCCCCCCCCCCCCHHHHHH
AMYAAQLYAAARRDIADLPAQIAAGDIAPIGHWLRQHIWSHGSRWDTDTLIRNVCGEPLN
HHHHHHHHHHHHHHHHHCCHHHHCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHCCCCCC
PDYLRRHLEQRYLGSMRDSLR
HHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7765282; 1369078 [H]