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The map label for this gene is ldhA [H]

Identifier: 238919834

GI number: 238919834

Start: 1864375

End: 1865367

Strand: Reverse

Name: ldhA [H]

Synonym: NT01EI_1938

Alternate gene names: 238919834

Gene position: 1865367-1864375 (Counterclockwise)

Preceding gene: 238919839

Following gene: 238919831

Centisome position: 48.93

GC content: 56.7

Gene sequence:

>993_bases
ATGAAACTCGCCATCTACAGCACCAAACAATACGACCGAAAATATATTGAGCTGGTAAACAAGCAGTTTGGCTTTGAACT
GGAGTTTTTTGACTTTCTGCTGCGCCCTCAGACGGTGAAAACCGCCCAGGGGTTCGATGCGGTCTGCATTTTTGTCAACG
ATGACGGCGGACGCGAAGTGCTGGAGCAGCTGGCGGCCATGGGCATTCGTATCGTCGCCCTGCGCTGCGCCGGTTTTAAC
AATGTCGATCTGAACGCCGCCAAAGCGCTGGGTATCGAAGTAGTCCGCGTACCGGCCTACTCGCCAGAGGCAGTCGCCGA
GCATACCGTTGGCATGATGCTGACCCTTAACCGTCGAATTCACCGCGCCTATCAGCGCACCCGCGACGCTAACTTCTCGC
TTGAGGGGTTGATCGGATTCAACATGCATAACCGTACCGCCGGCGTTATCGGCACCGGGAAGATCGGGATCGCCACGATG
CGCATCCTCAAGGGGTTCGGGATGCGTATCCTGGCATTCGACCCCTACCCCAACCCACAGGCGCTGGAGCTCGGCGCGGA
GTATGTCGATCTGAAAACCCTGTATGCCCAGTCCGACGTCATTACCCTGCACTGCCCGCTGACGCCGGAGAACCACCATC
TGCTAAACCAGGATGCCTTTGCCCATATGAAAGACGGGGTGATGATCATCAATACCAGTCGCGGCGCCCTGATCGACTCT
TGCGCCGCTATCGATGCGCTGAAGCAACAGAAGATCGGGGCCCTGGGCATGGACGTGTATGAAAATGAGCGCGATCTGTT
CTTTGAAGACAAATCCAACGATGTGATCCAGGACGACGTGTTCCGCCGCCTCTCCGCCTGCCATAACGTGCTGTTTACCG
GCCACCAGGCTTTCCTGACCGAAGAGGCATTGACCAGCATCTCACTGACCACCCTGCAGAACATCGATGATCTGATCCAT
CATCGCCACTGCCCGAATCGCCTGAACGCCTGA

Upstream 100 bases:

>100_bases
TCCTAACAGGAAAAAATGATTTACATCACACTTCCCCTGTCATTTGGTCCACCTACCTATTTTTGCTATGATGATCACAA
TTTCAGCTGGGAGAATATTG

Downstream 100 bases:

>100_bases
TAGCTGTACAGCCATGACACACAAAAAAACCCGCTACGGCGGGTTTTTTTGTTCACAACTTTCGGCAGCGGCCTATACTA
GTGTCTCCTGCGTATAGCGC

Product: D-isomer specific 2-hydroxyacid dehydrogenase family protein

Products: NA

Alternate protein names: D-LDH; Fermentative lactate dehydrogenase [H]

Number of amino acids: Translated: 330; Mature: 330

Protein sequence:

>330_residues
MKLAIYSTKQYDRKYIELVNKQFGFELEFFDFLLRPQTVKTAQGFDAVCIFVNDDGGREVLEQLAAMGIRIVALRCAGFN
NVDLNAAKALGIEVVRVPAYSPEAVAEHTVGMMLTLNRRIHRAYQRTRDANFSLEGLIGFNMHNRTAGVIGTGKIGIATM
RILKGFGMRILAFDPYPNPQALELGAEYVDLKTLYAQSDVITLHCPLTPENHHLLNQDAFAHMKDGVMIINTSRGALIDS
CAAIDALKQQKIGALGMDVYENERDLFFEDKSNDVIQDDVFRRLSACHNVLFTGHQAFLTEEALTSISLTTLQNIDDLIH
HRHCPNRLNA

Sequences:

>Translated_330_residues
MKLAIYSTKQYDRKYIELVNKQFGFELEFFDFLLRPQTVKTAQGFDAVCIFVNDDGGREVLEQLAAMGIRIVALRCAGFN
NVDLNAAKALGIEVVRVPAYSPEAVAEHTVGMMLTLNRRIHRAYQRTRDANFSLEGLIGFNMHNRTAGVIGTGKIGIATM
RILKGFGMRILAFDPYPNPQALELGAEYVDLKTLYAQSDVITLHCPLTPENHHLLNQDAFAHMKDGVMIINTSRGALIDS
CAAIDALKQQKIGALGMDVYENERDLFFEDKSNDVIQDDVFRRLSACHNVLFTGHQAFLTEEALTSISLTTLQNIDDLIH
HRHCPNRLNA
>Mature_330_residues
MKLAIYSTKQYDRKYIELVNKQFGFELEFFDFLLRPQTVKTAQGFDAVCIFVNDDGGREVLEQLAAMGIRIVALRCAGFN
NVDLNAAKALGIEVVRVPAYSPEAVAEHTVGMMLTLNRRIHRAYQRTRDANFSLEGLIGFNMHNRTAGVIGTGKIGIATM
RILKGFGMRILAFDPYPNPQALELGAEYVDLKTLYAQSDVITLHCPLTPENHHLLNQDAFAHMKDGVMIINTSRGALIDS
CAAIDALKQQKIGALGMDVYENERDLFFEDKSNDVIQDDVFRRLSACHNVLFTGHQAFLTEEALTSISLTTLQNIDDLIH
HRHCPNRLNA

Specific function: Fermentative lactate dehydrogenase [H]

COG id: COG1052

COG function: function code CHR; Lactate dehydrogenase and related dehydrogenases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the D-isomer specific 2-hydroxyacid dehydrogenase family [H]

Homologues:

Organism=Homo sapiens, GI23308577, Length=212, Percent_Identity=32.0754716981132, Blast_Score=128, Evalue=7e-30,
Organism=Homo sapiens, GI61743967, Length=255, Percent_Identity=34.1176470588235, Blast_Score=125, Evalue=7e-29,
Organism=Homo sapiens, GI4557497, Length=255, Percent_Identity=34.1176470588235, Blast_Score=125, Evalue=8e-29,
Organism=Homo sapiens, GI145580578, Length=256, Percent_Identity=32.8125, Blast_Score=120, Evalue=2e-27,
Organism=Homo sapiens, GI4557499, Length=256, Percent_Identity=32.8125, Blast_Score=120, Evalue=2e-27,
Organism=Homo sapiens, GI145580575, Length=256, Percent_Identity=32.8125, Blast_Score=118, Evalue=7e-27,
Organism=Homo sapiens, GI6912396, Length=204, Percent_Identity=34.3137254901961, Blast_Score=111, Evalue=1e-24,
Organism=Escherichia coli, GI1787645, Length=328, Percent_Identity=78.0487804878049, Blast_Score=546, Evalue=1e-157,
Organism=Escherichia coli, GI87082289, Length=238, Percent_Identity=32.3529411764706, Blast_Score=114, Evalue=7e-27,
Organism=Escherichia coli, GI1789279, Length=248, Percent_Identity=29.8387096774194, Blast_Score=93, Evalue=2e-20,
Organism=Escherichia coli, GI1788660, Length=210, Percent_Identity=27.1428571428571, Blast_Score=64, Evalue=1e-11,
Organism=Caenorhabditis elegans, GI17532191, Length=250, Percent_Identity=28.8, Blast_Score=117, Evalue=6e-27,
Organism=Caenorhabditis elegans, GI25147481, Length=277, Percent_Identity=29.2418772563177, Blast_Score=107, Evalue=8e-24,
Organism=Saccharomyces cerevisiae, GI6322116, Length=310, Percent_Identity=27.741935483871, Blast_Score=117, Evalue=2e-27,
Organism=Saccharomyces cerevisiae, GI6320925, Length=307, Percent_Identity=27.6872964169381, Blast_Score=117, Evalue=3e-27,
Organism=Saccharomyces cerevisiae, GI6324055, Length=218, Percent_Identity=33.4862385321101, Blast_Score=114, Evalue=2e-26,
Organism=Saccharomyces cerevisiae, GI6324964, Length=225, Percent_Identity=28, Blast_Score=92, Evalue=1e-19,
Organism=Saccharomyces cerevisiae, GI6324980, Length=145, Percent_Identity=27.5862068965517, Blast_Score=70, Evalue=4e-13,
Organism=Saccharomyces cerevisiae, GI6325144, Length=181, Percent_Identity=27.6243093922652, Blast_Score=66, Evalue=1e-11,
Organism=Drosophila melanogaster, GI24646446, Length=287, Percent_Identity=33.7979094076655, Blast_Score=131, Evalue=5e-31,
Organism=Drosophila melanogaster, GI24646448, Length=287, Percent_Identity=33.7979094076655, Blast_Score=131, Evalue=5e-31,
Organism=Drosophila melanogaster, GI24646452, Length=287, Percent_Identity=33.7979094076655, Blast_Score=131, Evalue=5e-31,
Organism=Drosophila melanogaster, GI24646450, Length=287, Percent_Identity=33.7979094076655, Blast_Score=131, Evalue=5e-31,
Organism=Drosophila melanogaster, GI62472511, Length=287, Percent_Identity=33.7979094076655, Blast_Score=130, Evalue=1e-30,
Organism=Drosophila melanogaster, GI19921140, Length=299, Percent_Identity=26.4214046822742, Blast_Score=117, Evalue=8e-27,
Organism=Drosophila melanogaster, GI28574286, Length=276, Percent_Identity=30.4347826086957, Blast_Score=106, Evalue=3e-23,
Organism=Drosophila melanogaster, GI45552429, Length=239, Percent_Identity=28.8702928870293, Blast_Score=93, Evalue=2e-19,
Organism=Drosophila melanogaster, GI24585514, Length=239, Percent_Identity=28.8702928870293, Blast_Score=93, Evalue=3e-19,
Organism=Drosophila melanogaster, GI28574282, Length=239, Percent_Identity=28.8702928870293, Blast_Score=93, Evalue=3e-19,
Organism=Drosophila melanogaster, GI28574284, Length=239, Percent_Identity=28.8702928870293, Blast_Score=92, Evalue=3e-19,
Organism=Drosophila melanogaster, GI28571528, Length=242, Percent_Identity=28.9256198347107, Blast_Score=92, Evalue=4e-19,
Organism=Drosophila melanogaster, GI45551003, Length=239, Percent_Identity=28.8702928870293, Blast_Score=92, Evalue=5e-19,
Organism=Drosophila melanogaster, GI24585516, Length=203, Percent_Identity=26.1083743842365, Blast_Score=73, Evalue=3e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006139
- InterPro:   IPR006140
- InterPro:   IPR016040 [H]

Pfam domain/function: PF00389 2-Hacid_dh; PF02826 2-Hacid_dh_C [H]

EC number: =1.1.1.28 [H]

Molecular weight: Translated: 36945; Mature: 36945

Theoretical pI: Translated: 6.51; Mature: 6.51

Prosite motif: PS00065 D_2_HYDROXYACID_DH_1 ; PS00670 D_2_HYDROXYACID_DH_2 ; PS00671 D_2_HYDROXYACID_DH_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLAIYSTKQYDRKYIELVNKQFGFELEFFDFLLRPQTVKTAQGFDAVCIFVNDDGGREV
CEEEEECCHHHHHHHHHHHHHHHCCEEHHHHHHHCCCCCCCCCCCCEEEEEEECCCCHHH
LEQLAAMGIRIVALRCAGFNNVDLNAAKALGIEVVRVPAYSPEAVAEHTVGMMLTLNRRI
HHHHHHCCEEEEEEEECCCCCCCCCHHHHCCEEEEEECCCCCHHHHHHHHHHEEEHHHHH
HRAYQRTRDANFSLEGLIGFNMHNRTAGVIGTGKIGIATMRILKGFGMRILAFDPYPNPQ
HHHHHHHCCCCCEEEEEEEECCCCCCCCEEECCCHHHHHHHHHHHCCCEEEEECCCCCCH
ALELGAEYVDLKTLYAQSDVITLHCPLTPENHHLLNQDAFAHMKDGVMIINTSRGALIDS
HHHHCCHHHHHHHEEECCCEEEEECCCCCCCCCCCCCHHHHHHCCCEEEEECCCCCHHHH
CAAIDALKQQKIGALGMDVYENERDLFFEDKSNDVIQDDVFRRLSACHNVLFTGHQAFLT
HHHHHHHHHHHCCCCCCCEECCCCCEEEECCCCCCHHHHHHHHHHHHHHHHHCCCHHHHH
EEALTSISLTTLQNIDDLIHHRHCPNRLNA
HHHHHHHHHHHHHHHHHHHHHCCCCHHCCC
>Mature Secondary Structure
MKLAIYSTKQYDRKYIELVNKQFGFELEFFDFLLRPQTVKTAQGFDAVCIFVNDDGGREV
CEEEEECCHHHHHHHHHHHHHHHCCEEHHHHHHHCCCCCCCCCCCCEEEEEEECCCCHHH
LEQLAAMGIRIVALRCAGFNNVDLNAAKALGIEVVRVPAYSPEAVAEHTVGMMLTLNRRI
HHHHHHCCEEEEEEEECCCCCCCCCHHHHCCEEEEEECCCCCHHHHHHHHHHEEEHHHHH
HRAYQRTRDANFSLEGLIGFNMHNRTAGVIGTGKIGIATMRILKGFGMRILAFDPYPNPQ
HHHHHHHCCCCCEEEEEEEECCCCCCCCEEECCCHHHHHHHHHHHCCCEEEEECCCCCCH
ALELGAEYVDLKTLYAQSDVITLHCPLTPENHHLLNQDAFAHMKDGVMIINTSRGALIDS
HHHHCCHHHHHHHEEECCCEEEEECCCCCCCCCCCCCHHHHHHCCCEEEEECCCCCHHHH
CAAIDALKQQKIGALGMDVYENERDLFFEDKSNDVIQDDVFRRLSACHNVLFTGHQAFLT
HHHHHHHHHHHCCCCCCCEECCCCCEEEECCCCCCHHHHHHHHHHHHHHHHHCCCHHHHH
EEALTSISLTTLQNIDDLIHHRHCPNRLNA
HHHHHHHHHHHHHHHHHHHHHCCCCHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9025293; 9097039; 9278503; 8349564 [H]