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The map label for this gene is pheT [H]

Identifier: 238919787

GI number: 238919787

Start: 1820244

End: 1822631

Strand: Direct

Name: pheT [H]

Synonym: NT01EI_1891

Alternate gene names: 238919787

Gene position: 1820244-1822631 (Clockwise)

Preceding gene: 238919786

Following gene: 238919788

Centisome position: 47.75

GC content: 59.97

Gene sequence:

>2388_bases
ATGAAATTCAGTGAACTTTGGTTGCGCGAGTGGGTAAATCCTGCCATCAGCAGCGATGCATTGTCCGAACAAATTACCAT
GGCTGGCCTGGAAGTGGATGGCGTAGAGCCGGTTGCCGGCGCTTTCCATGGTGTGGTGGTCGGTGAGGTGGTCGAGTGCC
ATCAACATCCCAATGCCGATAAGCTACGGGTCACCAAGGTCAGCGTCGGCGGCGAGCGCTTGCTGGATATCGTCTGCGGT
GCGCCGAACTGCCGTCAGGGACTGCGTGTGGCCGTTGCTACTATCGGAGCTGTTCTGCCGGGCGATTTCAAAATCAAAGC
GGCTAAGCTGCGCGGCGAACCCTCCGAAGGGATGCTGTGCTCTTTCTCTGAGCTGGGTATCTGTGAGGATCACAGCGGTA
TCATCGAACTGCCGCAGGATGCGCCGATCGGCTGTGATCTGCGGGAGTATCTGCAGCTGAATGACAACGCGATCGAAATC
AGTGTGACCCCTAACCGGGCCGATTGCCTCGGTATTCTGGGCGTTGCCCGCGATGTCGCGGTGCTTAACCGCCTGCCGCT
GAATATGCCGCAAGCGGCGCCGGTAGCGGCCAGCATCGATGAGCGCTTCCCGATCGCCGTTGAGGCGCCAGAGGCCTGCC
CGCGTTACCTTGGCCGCGTGGTGCGCGGCATCGACGTCGCGGCGAAGACGCCGTTGTGGATGAGTGAAAAGCTGCGTCGC
TGCGGCATTCGATCCATCGATCCGGTGGTGGACGTGACCAATTACGTTCTGCTGGAGTTGGGGCAGCCGATGCACGCCTT
CGATCTCAACCGCCTGCAGGGTGGGATCGTGGTACGTATGGCCCGTGATGGGGAGACCCTGACGCTGCTGGATGGCAGCC
AGGCGACGCTGGGTAGCGATACGCTGGTCATTGCCGACCATCAGCAGGCGCTGGCGTTGGGCGGCATTTTTGGCGGTGAA
CATTCCGGTGTTAACGCGGAAACCCGAGACGTGCTGCTGGAGTGCGCCTATTTTAACCCGCTGGCGATTACCGGCCGCGC
CCGTCGCTATGGGTTGCACACCGACGCGTCGCATCGCTATGAGCGCGGCGTCGATCCGCAGCTGCAGCAGCGTGCCATGG
AGCGCGCTACCGAGCTGCTGCTGGCGCTGTGCGGCGGCCAGGCCGGTCCGGTTATGGATGTGACCAACGATGCCTACCTA
CCGCAGCCGGCCAACATTGTGCTGCAGCGCAGCAAACTGGATCGTCTGATCGGCCATCATATCGATGATGTGCAGGTGCT
GGATATTCTGCAGCGTCTGGGCTGCCAGGTAACGGCAGTGGATGGCGGATGGCAGGCTGTCGCGCCGAGCTGGCGTTTTG
ACATGGCCATTGAAGAGGATCTGATCGAAGAGGTGGCGCGTGTCTACGGTTATAACAATATTCCGGATGTGCCACTGCGT
GCTGATCTGGTGATGACCGCACACCGTGAGGCGAATCTGCCCCTGAAGCGGGTCAAGACCCTGCTGGTCGATCGTGGCTA
CCAAGAGGCTATCACCTACAGCTTTGTCGATCCAAAAGTACAGGCGCTGTTGCACCCGCAGCAGCAGGCGCTGATCCTGC
CAAACCCGATCTCCGTTGATATGTCCGCCATGCGCCTGTCGCTGTGGACCGGTCTGCTGGGCGCCGTGGTATATAACCAG
AACCGCCAGCAGTCACGGGTGCGTCTGTTTGAAAGCGGCCTGCGCTTTGTTCCGGACGAGGGGGCGAATCTGGGGATCCG
CCAGGACGTGATGCTGGCCGGGGTGATCGCCGGTCATCGCGACGAAGAGCACTGGGGCATCGAGCGCCAGGCCGTCGATT
TCTACGATCTGAAGGGCGACCTGGAGGCGGTGCTGGAGCTGACCGGTAAACTGGACGCGGTGCAGTTCCGCGCCGAAGCC
CACGATGCCCTGCATCCGGGGCAGAGCGCCGCGCTGTATCTGCATGGCGAGCGAATTGGTTTCATTGGCGTGGTACATCC
GGAGCTGGAGCGTAAGCTGGATCTCAACGGTCGTACAGTCGTGTTTGAGGTGTGCTGGAGTGCTCTGGCAGAGCGTCTGA
TTCCACAGGCGCGCGAGATCTCCCGCTATCCCGCGAACCGCCGTGATATCGCGGTTGTGGTGGCTGAAAATGTGTCGGCG
GAAGATGTTTTGGCTGAGTGTAAGAAAGTTGGCGCAAATCAGGTAGTTGGCGTAAACTTATTTGACGTGTATTGTGGTAA
GGGGGTTGCAGAGGGTCATAAGAGCCTCGCGATCAGTCTGGTGTTGCAGGATACCACGCGTACACTGGAAGAAGTGGAAA
TTGCCGCTACCGTCGCAAAATGCGTAGAGGCGTTAAAACAGCGATTCCAAGCATCCTTGAGGGATTGA

Upstream 100 bases:

>100_bases
AGCGACTGACGATGCTGCGTTACGGTGTCACTGATTTGCGCGCATTTTTCGAAAACGATCTGCGTTTCCTCAAACAGTTC
AAATAAGGCAGGATTAACAC

Downstream 100 bases:

>100_bases
ATCTATGGCGCTTACAAAAGCTGAAATGTCTGAACACCTGTTTGAAAAGCTTGGGCTGAGCAAACGGGACGCCAAAGACC
TCGTCGAGCTGTTTTTCGAA

Product: phenylalanyl-tRNA synthetase subunit beta

Products: NA

Alternate protein names: Phenylalanine--tRNA ligase beta chain; PheRS [H]

Number of amino acids: Translated: 795; Mature: 795

Protein sequence:

>795_residues
MKFSELWLREWVNPAISSDALSEQITMAGLEVDGVEPVAGAFHGVVVGEVVECHQHPNADKLRVTKVSVGGERLLDIVCG
APNCRQGLRVAVATIGAVLPGDFKIKAAKLRGEPSEGMLCSFSELGICEDHSGIIELPQDAPIGCDLREYLQLNDNAIEI
SVTPNRADCLGILGVARDVAVLNRLPLNMPQAAPVAASIDERFPIAVEAPEACPRYLGRVVRGIDVAAKTPLWMSEKLRR
CGIRSIDPVVDVTNYVLLELGQPMHAFDLNRLQGGIVVRMARDGETLTLLDGSQATLGSDTLVIADHQQALALGGIFGGE
HSGVNAETRDVLLECAYFNPLAITGRARRYGLHTDASHRYERGVDPQLQQRAMERATELLLALCGGQAGPVMDVTNDAYL
PQPANIVLQRSKLDRLIGHHIDDVQVLDILQRLGCQVTAVDGGWQAVAPSWRFDMAIEEDLIEEVARVYGYNNIPDVPLR
ADLVMTAHREANLPLKRVKTLLVDRGYQEAITYSFVDPKVQALLHPQQQALILPNPISVDMSAMRLSLWTGLLGAVVYNQ
NRQQSRVRLFESGLRFVPDEGANLGIRQDVMLAGVIAGHRDEEHWGIERQAVDFYDLKGDLEAVLELTGKLDAVQFRAEA
HDALHPGQSAALYLHGERIGFIGVVHPELERKLDLNGRTVVFEVCWSALAERLIPQAREISRYPANRRDIAVVVAENVSA
EDVLAECKKVGANQVVGVNLFDVYCGKGVAEGHKSLAISLVLQDTTRTLEEVEIAATVAKCVEALKQRFQASLRD

Sequences:

>Translated_795_residues
MKFSELWLREWVNPAISSDALSEQITMAGLEVDGVEPVAGAFHGVVVGEVVECHQHPNADKLRVTKVSVGGERLLDIVCG
APNCRQGLRVAVATIGAVLPGDFKIKAAKLRGEPSEGMLCSFSELGICEDHSGIIELPQDAPIGCDLREYLQLNDNAIEI
SVTPNRADCLGILGVARDVAVLNRLPLNMPQAAPVAASIDERFPIAVEAPEACPRYLGRVVRGIDVAAKTPLWMSEKLRR
CGIRSIDPVVDVTNYVLLELGQPMHAFDLNRLQGGIVVRMARDGETLTLLDGSQATLGSDTLVIADHQQALALGGIFGGE
HSGVNAETRDVLLECAYFNPLAITGRARRYGLHTDASHRYERGVDPQLQQRAMERATELLLALCGGQAGPVMDVTNDAYL
PQPANIVLQRSKLDRLIGHHIDDVQVLDILQRLGCQVTAVDGGWQAVAPSWRFDMAIEEDLIEEVARVYGYNNIPDVPLR
ADLVMTAHREANLPLKRVKTLLVDRGYQEAITYSFVDPKVQALLHPQQQALILPNPISVDMSAMRLSLWTGLLGAVVYNQ
NRQQSRVRLFESGLRFVPDEGANLGIRQDVMLAGVIAGHRDEEHWGIERQAVDFYDLKGDLEAVLELTGKLDAVQFRAEA
HDALHPGQSAALYLHGERIGFIGVVHPELERKLDLNGRTVVFEVCWSALAERLIPQAREISRYPANRRDIAVVVAENVSA
EDVLAECKKVGANQVVGVNLFDVYCGKGVAEGHKSLAISLVLQDTTRTLEEVEIAATVAKCVEALKQRFQASLRD
>Mature_795_residues
MKFSELWLREWVNPAISSDALSEQITMAGLEVDGVEPVAGAFHGVVVGEVVECHQHPNADKLRVTKVSVGGERLLDIVCG
APNCRQGLRVAVATIGAVLPGDFKIKAAKLRGEPSEGMLCSFSELGICEDHSGIIELPQDAPIGCDLREYLQLNDNAIEI
SVTPNRADCLGILGVARDVAVLNRLPLNMPQAAPVAASIDERFPIAVEAPEACPRYLGRVVRGIDVAAKTPLWMSEKLRR
CGIRSIDPVVDVTNYVLLELGQPMHAFDLNRLQGGIVVRMARDGETLTLLDGSQATLGSDTLVIADHQQALALGGIFGGE
HSGVNAETRDVLLECAYFNPLAITGRARRYGLHTDASHRYERGVDPQLQQRAMERATELLLALCGGQAGPVMDVTNDAYL
PQPANIVLQRSKLDRLIGHHIDDVQVLDILQRLGCQVTAVDGGWQAVAPSWRFDMAIEEDLIEEVARVYGYNNIPDVPLR
ADLVMTAHREANLPLKRVKTLLVDRGYQEAITYSFVDPKVQALLHPQQQALILPNPISVDMSAMRLSLWTGLLGAVVYNQ
NRQQSRVRLFESGLRFVPDEGANLGIRQDVMLAGVIAGHRDEEHWGIERQAVDFYDLKGDLEAVLELTGKLDAVQFRAEA
HDALHPGQSAALYLHGERIGFIGVVHPELERKLDLNGRTVVFEVCWSALAERLIPQAREISRYPANRRDIAVVVAENVSA
EDVLAECKKVGANQVVGVNLFDVYCGKGVAEGHKSLAISLVLQDTTRTLEEVEIAATVAKCVEALKQRFQASLRD

Specific function: Unknown

COG id: COG0072

COG function: function code J; Phenylalanyl-tRNA synthetase beta subunit

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 tRNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1788006, Length=795, Percent_Identity=80.7547169811321, Blast_Score=1298, Evalue=0.0,

Paralogues:

None

Copy number: 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005146
- InterPro:   IPR005147
- InterPro:   IPR009061
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR004532
- InterPro:   IPR020825
- InterPro:   IPR005121
- InterPro:   IPR002547 [H]

Pfam domain/function: PF03483 B3_4; PF03484 B5; PF03147 FDX-ACB; PF01588 tRNA_bind [H]

EC number: =6.1.1.20 [H]

Molecular weight: Translated: 86942; Mature: 86942

Theoretical pI: Translated: 5.06; Mature: 5.06

Prosite motif: PS50886 TRBD

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKFSELWLREWVNPAISSDALSEQITMAGLEVDGVEPVAGAFHGVVVGEVVECHQHPNAD
CCHHHHHHHHHCCHHHCHHHHHHHHEEECCEECCCCHHHHHHHHHHHHHHHHHHCCCCCC
KLRVTKVSVGGERLLDIVCGAPNCRQGLRVAVATIGAVLPGDFKIKAAKLRGEPSEGMLC
CEEEEEEECCHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCCCCEE
SFSELGICEDHSGIIELPQDAPIGCDLREYLQLNDNAIEISVTPNRADCLGILGVARDVA
EHHHCCCCCCCCCCEECCCCCCCCCCHHHHHCCCCCEEEEEECCCCHHHHHHHHHHHHHH
VLNRLPLNMPQAAPVAASIDERFPIAVEAPEACPRYLGRVVRGIDVAAKTPLWMSEKLRR
HHHHCCCCCCCCCCCHHCCCCCCCEEEECHHHHHHHHHHHHHCCCHHCCCCCHHHHHHHH
CGIRSIDPVVDVTNYVLLELGQPMHAFDLNRLQGGIVVRMARDGETLTLLDGSQATLGSD
CCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHCCCCEEEEEECCCCEEEEECCCCCCCCCC
TLVIADHQQALALGGIFGGEHSGVNAETRDVLLECAYFNPLAITGRARRYGLHTDASHRY
EEEEECCCHHHHCCCCCCCCCCCCCCHHHHHHEEEECCCCEEEECCCCEECCCCCCCHHH
ERGVDPQLQQRAMERATELLLALCGGQAGPVMDVTNDAYLPQPANIVLQRSKLDRLIGHH
HCCCCHHHHHHHHHHHHHHHHHHHCCCCCCEEECCCCCCCCCCHHHEEEHHHHHHHHCCC
IDDVQVLDILQRLGCQVTAVDGGWQAVAPSWRFDMAIEEDLIEEVARVYGYNNIPDVPLR
CCCHHHHHHHHHHCCEEEEECCCCEECCCCCEECCHHHHHHHHHHHHHCCCCCCCCCCCC
ADLVMTAHREANLPLKRVKTLLVDRGYQEAITYSFVDPKVQALLHPQQQALILPNPISVD
EEEEEEECCCCCCCHHHHHHHHHCCCHHHHHEEEECCHHHHHHHCCCCCEEECCCCCCCC
MSAMRLSLWTGLLGAVVYNQNRQQSRVRLFESGLRFVPDEGANLGIRQDVMLAGVIAGHR
HHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHCCC
DEEHWGIERQAVDFYDLKGDLEAVLELTGKLDAVQFRAEAHDALHPGQSAALYLHGERIG
CCHHCCCCHHCCEEEECCHHHHHHHHHHCCCHHEEEHHHHHHHCCCCCCEEEEEECCCEE
FIGVVHPELERKLDLNGRTVVFEVCWSALAERLIPQAREISRYPANRRDIAVVVAENVSA
EEEEECHHHHHHCCCCCCEEHHHHHHHHHHHHHCCHHHHHHHCCCCCCCEEEEEECCCCH
EDVLAECKKVGANQVVGVNLFDVYCGKGVAEGHKSLAISLVLQDTTRTLEEVEIAATVAK
HHHHHHHHHCCCCCEEEEEEEEHHCCCCHHCCCCEEEEEEEEECHHHHHHHHHHHHHHHH
CVEALKQRFQASLRD
HHHHHHHHHHHHCCC
>Mature Secondary Structure
MKFSELWLREWVNPAISSDALSEQITMAGLEVDGVEPVAGAFHGVVVGEVVECHQHPNAD
CCHHHHHHHHHCCHHHCHHHHHHHHEEECCEECCCCHHHHHHHHHHHHHHHHHHCCCCCC
KLRVTKVSVGGERLLDIVCGAPNCRQGLRVAVATIGAVLPGDFKIKAAKLRGEPSEGMLC
CEEEEEEECCHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCCCCEE
SFSELGICEDHSGIIELPQDAPIGCDLREYLQLNDNAIEISVTPNRADCLGILGVARDVA
EHHHCCCCCCCCCCEECCCCCCCCCCHHHHHCCCCCEEEEEECCCCHHHHHHHHHHHHHH
VLNRLPLNMPQAAPVAASIDERFPIAVEAPEACPRYLGRVVRGIDVAAKTPLWMSEKLRR
HHHHCCCCCCCCCCCHHCCCCCCCEEEECHHHHHHHHHHHHHCCCHHCCCCCHHHHHHHH
CGIRSIDPVVDVTNYVLLELGQPMHAFDLNRLQGGIVVRMARDGETLTLLDGSQATLGSD
CCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHCCCCEEEEEECCCCEEEEECCCCCCCCCC
TLVIADHQQALALGGIFGGEHSGVNAETRDVLLECAYFNPLAITGRARRYGLHTDASHRY
EEEEECCCHHHHCCCCCCCCCCCCCCHHHHHHEEEECCCCEEEECCCCEECCCCCCCHHH
ERGVDPQLQQRAMERATELLLALCGGQAGPVMDVTNDAYLPQPANIVLQRSKLDRLIGHH
HCCCCHHHHHHHHHHHHHHHHHHHCCCCCCEEECCCCCCCCCCHHHEEEHHHHHHHHCCC
IDDVQVLDILQRLGCQVTAVDGGWQAVAPSWRFDMAIEEDLIEEVARVYGYNNIPDVPLR
CCCHHHHHHHHHHCCEEEEECCCCEECCCCCEECCHHHHHHHHHHHHHCCCCCCCCCCCC
ADLVMTAHREANLPLKRVKTLLVDRGYQEAITYSFVDPKVQALLHPQQQALILPNPISVD
EEEEEEECCCCCCCHHHHHHHHHCCCHHHHHEEEECCHHHHHHHCCCCCEEECCCCCCCC
MSAMRLSLWTGLLGAVVYNQNRQQSRVRLFESGLRFVPDEGANLGIRQDVMLAGVIAGHR
HHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHCCC
DEEHWGIERQAVDFYDLKGDLEAVLELTGKLDAVQFRAEAHDALHPGQSAALYLHGERIG
CCHHCCCCHHCCEEEECCHHHHHHHHHHCCCHHEEEHHHHHHHCCCCCCEEEEEECCCEE
FIGVVHPELERKLDLNGRTVVFEVCWSALAERLIPQAREISRYPANRRDIAVVVAENVSA
EEEEECHHHHHHCCCCCCEEHHHHHHHHHHHHHCCHHHHHHHCCCCCCCEEEEEECCCCH
EDVLAECKKVGANQVVGVNLFDVYCGKGVAEGHKSLAISLVLQDTTRTLEEVEIAATVAK
HHHHHHHHHCCCCCEEEEEEEEHHCCCCHHCCCCEEEEEEEEECHHHHHHHHHHHHHHHH
CVEALKQRFQASLRD
HHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11586360; 12142430 [H]