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The map label for this gene is pfkB [H]

Identifier: 238919774

GI number: 238919774

Start: 1810983

End: 1811936

Strand: Direct

Name: pfkB [H]

Synonym: NT01EI_1878

Alternate gene names: 238919774

Gene position: 1810983-1811936 (Clockwise)

Preceding gene: 238919770

Following gene: 238919776

Centisome position: 47.5

GC content: 58.7

Gene sequence:

>954_bases
ATGAATTCTGTCTATACCCTTACGCTGTCTCCCTCGCTGGACTGTTCGACCAGCACGGATAAAATATATCCGGAGGGTAA
GTTACGCTGCAGTCAGGCCATATACCAACCGGGTGGCGGTGGGGTTAACGTGGCCAGGGCCATTTACCGCATGGGGGGAG
GCGCAGTGGCCATCTATCCTGCGGGAGGATCGACCGGTGCATACTTAACCACACTGCTGTTGGCGGAAGGGATTGCGGTT
GATGTGGTAGAGGTACAGAGCTGGACGCGTCAAAATATGCATATCAATGTGACGGCTACCGGCGAGCAGTTTCGTTTTGT
GATGCCTGGCGCGGATCTGACGCCGCGGGAATTCGATCTTCTGACGCAACGACTTGATCAGATCCCCGCGGATGCTTTTT
TGGTGGTGAGCGGAAGTTTGCCGGAAAGCATTACGCCGCAGCGGTTTGCCGCACTGCTGCAGTACGTTTGTCAGCGTGGT
ATTCGCTGCGTTGTCGATACCTCGGGCGATGCCTTGATGACGGCGGTGAGTCAGGGAGGACTGGCGCTGATTAAACCTAA
TCAAAAGGAGCTGGCGACGTTGAGCGGGCGGGATATGACGCAGCCGGCTTCGGTTTTGCAGGCGGCCCATGCGCTGGTAT
TGCAGGGCGTTGCGCAGTATGTGGTGGTTTCCCTGGGGGCTCAGGGGGCGATTGCCGTTGATCGTCAAGCCTGGATTCAG
GTGACGCCGCCGCCGATTAAGGCGCTGACGACGGTGGGAGCCGGTGACAGTATGGTCGCCGCGATGACGCTGCAGCTGGC
TCTGGGAGGATCGCTGAGCACACTGGCGATTGGCGGTGTTGCGGCCGGAACCGCGGCAACGCTACGCCACGGTACCGAGC
TATGTCAGGCGAGCGATGTCGCGGAGATTGCAGCCTATATTGAGGCCCATCACCCGCAGTCGCATCCATTCTAA

Upstream 100 bases:

>100_bases
TCAAGCTCCGCGGAATCCCCTATAGGTGATCGCGCACTGCAGGATGAGGGGAGGCAAGGCATGCTACGCTGAAATAAACT
CTACCGATTAAGGCGCGCAT

Downstream 100 bases:

>100_bases
TGCGCCGCAGATGGCGTGGACAAAAAAAGACACGGGACGGCATCCGCCACCGGCTCATATCGATGGCACCTCGTCAGAGG
CTAGTCGGCCAGATGGGCCT

Product: 6-phosphofructokinase 2

Products: NA

Alternate protein names: Phosphofructokinase-2 [H]

Number of amino acids: Translated: 317; Mature: 317

Protein sequence:

>317_residues
MNSVYTLTLSPSLDCSTSTDKIYPEGKLRCSQAIYQPGGGGVNVARAIYRMGGGAVAIYPAGGSTGAYLTTLLLAEGIAV
DVVEVQSWTRQNMHINVTATGEQFRFVMPGADLTPREFDLLTQRLDQIPADAFLVVSGSLPESITPQRFAALLQYVCQRG
IRCVVDTSGDALMTAVSQGGLALIKPNQKELATLSGRDMTQPASVLQAAHALVLQGVAQYVVVSLGAQGAIAVDRQAWIQ
VTPPPIKALTTVGAGDSMVAAMTLQLALGGSLSTLAIGGVAAGTAATLRHGTELCQASDVAEIAAYIEAHHPQSHPF

Sequences:

>Translated_317_residues
MNSVYTLTLSPSLDCSTSTDKIYPEGKLRCSQAIYQPGGGGVNVARAIYRMGGGAVAIYPAGGSTGAYLTTLLLAEGIAV
DVVEVQSWTRQNMHINVTATGEQFRFVMPGADLTPREFDLLTQRLDQIPADAFLVVSGSLPESITPQRFAALLQYVCQRG
IRCVVDTSGDALMTAVSQGGLALIKPNQKELATLSGRDMTQPASVLQAAHALVLQGVAQYVVVSLGAQGAIAVDRQAWIQ
VTPPPIKALTTVGAGDSMVAAMTLQLALGGSLSTLAIGGVAAGTAATLRHGTELCQASDVAEIAAYIEAHHPQSHPF
>Mature_317_residues
MNSVYTLTLSPSLDCSTSTDKIYPEGKLRCSQAIYQPGGGGVNVARAIYRMGGGAVAIYPAGGSTGAYLTTLLLAEGIAV
DVVEVQSWTRQNMHINVTATGEQFRFVMPGADLTPREFDLLTQRLDQIPADAFLVVSGSLPESITPQRFAALLQYVCQRG
IRCVVDTSGDALMTAVSQGGLALIKPNQKELATLSGRDMTQPASVLQAAHALVLQGVAQYVVVSLGAQGAIAVDRQAWIQ
VTPPPIKALTTVGAGDSMVAAMTLQLALGGSLSTLAIGGVAAGTAATLRHGTELCQASDVAEIAAYIEAHHPQSHPF

Specific function: Key control step of glycolysis. [C]

COG id: COG1105

COG function: function code G; Fructose-1-phosphate kinase and related fructose-6-phosphate kinase (PfkB)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the carbohydrate kinase pfkB family [H]

Homologues:

Organism=Escherichia coli, GI1788017, Length=307, Percent_Identity=53.7459283387622, Blast_Score=339, Evalue=1e-94,
Organism=Escherichia coli, GI1788493, Length=268, Percent_Identity=31.3432835820896, Blast_Score=108, Evalue=5e-25,

Paralogues:

None

Copy number: 496 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011611
- InterPro:   IPR002173
- InterPro:   IPR017583 [H]

Pfam domain/function: PF00294 PfkB [H]

EC number: =2.7.1.11 [H]

Molecular weight: Translated: 33034; Mature: 33034

Theoretical pI: Translated: 5.93; Mature: 5.93

Prosite motif: PS00583 PFKB_KINASES_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNSVYTLTLSPSLDCSTSTDKIYPEGKLRCSQAIYQPGGGGVNVARAIYRMGGGAVAIYP
CCCEEEEEECCCCCCCCCCCCCCCCCCHHHHHHHCCCCCCCHHHHHHHHHCCCCEEEEEE
AGGSTGAYLTTLLLAEGIAVDVVEVQSWTRQNMHINVTATGEQFRFVMPGADLTPREFDL
CCCCCHHHHHHHHHHCCCEEEEEEHHHHCCCCEEEEEEECCCEEEEEECCCCCCCHHHHH
LTQRLDQIPADAFLVVSGSLPESITPQRFAALLQYVCQRGIRCVVDTSGDALMTAVSQGG
HHHHHHHCCCCEEEEEECCCCCCCCHHHHHHHHHHHHHCCCEEEEECCCCCEEHHHCCCC
LALIKPNQKELATLSGRDMTQPASVLQAAHALVLQGVAQYVVVSLGAQGAIAVDRQAWIQ
EEEECCCHHHHHEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEECCCEEEE
VTPPPIKALTTVGAGDSMVAAMTLQLALGGSLSTLAIGGVAAGTAATLRHGTELCQASDV
ECCCCHHHHEECCCCCHHHHHHHHHHHCCCCCHHEEECCHHCCHHHHHHHHHHHHHHCCH
AEIAAYIEAHHPQSHPF
HHHHHHHHHCCCCCCCC
>Mature Secondary Structure
MNSVYTLTLSPSLDCSTSTDKIYPEGKLRCSQAIYQPGGGGVNVARAIYRMGGGAVAIYP
CCCEEEEEECCCCCCCCCCCCCCCCCCHHHHHHHCCCCCCCHHHHHHHHHCCCCEEEEEE
AGGSTGAYLTTLLLAEGIAVDVVEVQSWTRQNMHINVTATGEQFRFVMPGADLTPREFDL
CCCCCHHHHHHHHHHCCCEEEEEEHHHHCCCCEEEEEEECCCEEEEEECCCCCCCHHHHH
LTQRLDQIPADAFLVVSGSLPESITPQRFAALLQYVCQRGIRCVVDTSGDALMTAVSQGG
HHHHHHHCCCCEEEEEECCCCCCCCHHHHHHHHHHHHHCCCEEEEECCCCCEEHHHCCCC
LALIKPNQKELATLSGRDMTQPASVLQAAHALVLQGVAQYVVVSLGAQGAIAVDRQAWIQ
EEEECCCHHHHHEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEECCCEEEE
VTPPPIKALTTVGAGDSMVAAMTLQLALGGSLSTLAIGGVAAGTAATLRHGTELCQASDV
ECCCCHHHHEECCCCCHHHHHHHHHHHCCCCCHHEEECCHHCCHHHHHHHHHHHHHHCCH
AEIAAYIEAHHPQSHPF
HHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 6235149; 9097039; 9278503; 6310120 [H]