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The map label for this gene is purT

Identifier: 238919758

GI number: 238919758

Start: 1797587

End: 1798765

Strand: Reverse

Name: purT

Synonym: NT01EI_1862

Alternate gene names: 238919758

Gene position: 1798765-1797587 (Counterclockwise)

Preceding gene: 238919771

Following gene: 238919756

Centisome position: 47.18

GC content: 64.97

Gene sequence:

>1179_bases
ATGCAGCCAATCGGAACCGCCCTTCGTCCCCACGCAACCCGCGTCATGCTGTTAGGCGCCGGTGAGCTCGGCAAAGAGGT
CGCTATTGAGAGCCAGCGTCTCGGTCTGGAAGTGATTGCCGTGGATCGCTATGCCGATGCGCCGGCCATGCAGGTCGCCC
ACCGTAGCCACGTCATGGATATGCGCGACGGCGACAGCCTGCGAGCGCTTATCGAGCGCGAGCGCCCTGACTATATCGTG
CCGGAAATAGAGGCCATCGCAACGCAAACGCTGCTGCAGCTGGAGAGCGACGGCCAGCGGGTCGTCCCCTGCGCCCGGGC
GGTACAGCTTACGATGAACCGTGAGGGGATCCGCCGCCTGGCGGCTGAGACCCTGGCGCTGCCCACCTCTCCCTATCGCT
TTGCCGACAGCATCGAGGCGTTTCGACAGGCGGTGACAGAAATAGGCTACCCCTGTTTCGCCAAGCCCGTCATGAGTTCA
TCGGGCAAAGGGCAGAGCATTCTACGCAACGACGACGATGTGGCACCCGCCTGGCAATACGCCCGCCAAGGCGCCCGCGG
TGACGCCGACAGCGTCATCGTCGAGGGCGAGGTCCGCTTTGATTTCGAAATAACCCTGTTGACGGTGCAGGCCAGCGACG
GTATCCACTTCTGCGCGCCGATCGGGCACCGCCAGCAGGAAGGCGACTACCGCGAGTCCTGGCAGCCGCAGGCGATGAGC
CCCGCCGCACTGGCGCGGGCGCAGGCGATCGCCGCCTCAGTCGTCCGGGCGCTGGGAGGATATGGTCTGTTTGGAGTAGA
GCTATTTGTCCGCGGCGATGAGGTGATCTTCAGCGAGGTCTCCCCCCGCCCCCACGACACCGGACTGGTCACGCTGGTCT
CACAGGATCTCTCTGAGTTTGCGCTGCATGTCCGCGCATTTCTCGGGCTACCAATCGGCGTGATCCGCCAGTTTGGCCCC
TGTGCATCTGCCGTACTGCTGCCCTCACTTCACAGCGACGACGTCACCTTCGCCAACCTCTCACAAGCGCTGCGCCCGGC
AACCCAGCTGCGCCTGTTTGGCAAGCCGCACATCGACGGCGTACGGCGACTCGGCGTCGCGCTGGCCTGGGGAGAGAGTA
CGGAAACAGCGCGCCAGCGCGCCAGGGACAGCGCGGCATCGGTGGTGATCCGCCCCTAA

Upstream 100 bases:

>100_bases
GCTTTATTGCTCATTGTAATAAAATCTTTCCCGTTGCCACGCAAACGGTTTCGTATATACTGCCGACGATAATTTCCCCG
AATGAACAGGAGCAGAATCC

Downstream 100 bases:

>100_bases
GCGTCCCCAAGCTACCGCCGCCGCCCCATCTTATTGCGATGCCTCGTCGTCGTCCGCCTCGTCAAAGGCCACGCGGATCT
GCTCGCCACGGTGGCTGGCG

Product: phosphoribosylglycinamide formyltransferase 2

Products: 5'-Phosphoribosyl-N-Formylglycinamide; Pyrophosphate; acetylphosphate; ADP [C]

Alternate protein names: GART 2; 5'-phosphoribosylglycinamide transformylase 2; Formate-dependent GAR transformylase; GAR transformylase 2

Number of amino acids: Translated: 392; Mature: 392

Protein sequence:

>392_residues
MQPIGTALRPHATRVMLLGAGELGKEVAIESQRLGLEVIAVDRYADAPAMQVAHRSHVMDMRDGDSLRALIERERPDYIV
PEIEAIATQTLLQLESDGQRVVPCARAVQLTMNREGIRRLAAETLALPTSPYRFADSIEAFRQAVTEIGYPCFAKPVMSS
SGKGQSILRNDDDVAPAWQYARQGARGDADSVIVEGEVRFDFEITLLTVQASDGIHFCAPIGHRQQEGDYRESWQPQAMS
PAALARAQAIAASVVRALGGYGLFGVELFVRGDEVIFSEVSPRPHDTGLVTLVSQDLSEFALHVRAFLGLPIGVIRQFGP
CASAVLLPSLHSDDVTFANLSQALRPATQLRLFGKPHIDGVRRLGVALAWGESTETARQRARDSAASVVIRP

Sequences:

>Translated_392_residues
MQPIGTALRPHATRVMLLGAGELGKEVAIESQRLGLEVIAVDRYADAPAMQVAHRSHVMDMRDGDSLRALIERERPDYIV
PEIEAIATQTLLQLESDGQRVVPCARAVQLTMNREGIRRLAAETLALPTSPYRFADSIEAFRQAVTEIGYPCFAKPVMSS
SGKGQSILRNDDDVAPAWQYARQGARGDADSVIVEGEVRFDFEITLLTVQASDGIHFCAPIGHRQQEGDYRESWQPQAMS
PAALARAQAIAASVVRALGGYGLFGVELFVRGDEVIFSEVSPRPHDTGLVTLVSQDLSEFALHVRAFLGLPIGVIRQFGP
CASAVLLPSLHSDDVTFANLSQALRPATQLRLFGKPHIDGVRRLGVALAWGESTETARQRARDSAASVVIRP
>Mature_392_residues
MQPIGTALRPHATRVMLLGAGELGKEVAIESQRLGLEVIAVDRYADAPAMQVAHRSHVMDMRDGDSLRALIERERPDYIV
PEIEAIATQTLLQLESDGQRVVPCARAVQLTMNREGIRRLAAETLALPTSPYRFADSIEAFRQAVTEIGYPCFAKPVMSS
SGKGQSILRNDDDVAPAWQYARQGARGDADSVIVEGEVRFDFEITLLTVQASDGIHFCAPIGHRQQEGDYRESWQPQAMS
PAALARAQAIAASVVRALGGYGLFGVELFVRGDEVIFSEVSPRPHDTGLVTLVSQDLSEFALHVRAFLGLPIGVIRQFGP
CASAVLLPSLHSDDVTFANLSQALRPATQLRLFGKPHIDGVRRLGVALAWGESTETARQRARDSAASVVIRP

Specific function: Catalyzes two reactions:the first one is the production of beta-formyl glycinamide ribonucleotide (GAR) from formate, ATP and beta GAR; the second, a side reaction, is the production of acetyl phosphate and ADP from acetate and ATP

COG id: COG0027

COG function: function code F; Formate-dependent phosphoribosylglycinamide formyltransferase (GAR transformylase)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ATP-grasp domain

Homologues:

Organism=Escherichia coli, GI1788155, Length=391, Percent_Identity=72.3785166240409, Blast_Score=576, Evalue=1e-166,
Organism=Escherichia coli, GI1786733, Length=193, Percent_Identity=26.9430051813472, Blast_Score=65, Evalue=6e-12,
Organism=Saccharomyces cerevisiae, GI6324702, Length=364, Percent_Identity=23.9010989010989, Blast_Score=96, Evalue=8e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PURT_EDWI9 (C5BGW0)

Other databases:

- EMBL:   CP001600
- RefSeq:   YP_002933273.1
- GeneID:   7961173
- GenomeReviews:   CP001600_GR
- KEGG:   eic:NT01EI_1862
- OMA:   PDYIVPE
- ProtClustDB:   CLSK2813184
- HAMAP:   MF_01643
- InterPro:   IPR011761
- InterPro:   IPR003135
- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR013817
- InterPro:   IPR016185
- InterPro:   IPR005862
- Gene3D:   G3DSA:3.30.1490.20
- Gene3D:   G3DSA:3.30.470.20
- Gene3D:   G3DSA:3.40.50.20
- PANTHER:   PTHR23047:SF2
- TIGRFAMs:   TIGR01142

Pfam domain/function: PF02222 ATP-grasp; SSF52440 PreATP-grasp-like

EC number: 2.1.2.- [C]

Molecular weight: Translated: 42637; Mature: 42637

Theoretical pI: Translated: 6.06; Mature: 6.06

Prosite motif: PS50975 ATP_GRASP

Important sites: BINDING 82-82 BINDING 114-114 BINDING 155-155 BINDING 203-203 BINDING 267-267 BINDING 279-279 BINDING 286-286 BINDING 355-355

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQPIGTALRPHATRVMLLGAGELGKEVAIESQRLGLEVIAVDRYADAPAMQVAHRSHVMD
CCCCCCCCCCCCEEEEEEECCCCCHHHHHHHHHCCEEEEEEECCCCCHHHHHHHHHHHCC
MRDGDSLRALIERERPDYIVPEIEAIATQTLLQLESDGQRVVPCARAVQLTMNREGIRRL
CCCCHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHCCCCEEECHHHHHHHHCCHHHHHHH
AAETLALPTSPYRFADSIEAFRQAVTEIGYPCFAKPVMSSSGKGQSILRNDDDVAPAWQY
HHHHHCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCHHHCCCCCCCHHHCCCCCCCHHHHH
ARQGARGDADSVIVEGEVRFDFEITLLTVQASDGIHFCAPIGHRQQEGDYRESWQPQAMS
HHCCCCCCCCCEEEECEEEEEEEEEEEEEECCCCEEEECCCCCCCCCCCHHHCCCCCCCC
PAALARAQAIAASVVRALGGYGLFGVELFVRGDEVIFSEVSPRPHDTGLVTLVSQDLSEF
HHHHHHHHHHHHHHHHHHCCCCEEEEEEEEECCEEEEECCCCCCCCCCEEEHHHHHHHHH
ALHVRAFLGLPIGVIRQFGPCASAVLLPSLHSDDVTFANLSQALRPATQLRLFGKPHIDG
HHHHHHHHCCCHHHHHHCCCHHHHHHCCCCCCCCCHHHHHHHHHCCHHHEEECCCCCHHH
VRRLGVALAWGESTETARQRARDSAASVVIRP
HHHHCEEEEECCCHHHHHHHHHHHHCCEEECC
>Mature Secondary Structure
MQPIGTALRPHATRVMLLGAGELGKEVAIESQRLGLEVIAVDRYADAPAMQVAHRSHVMD
CCCCCCCCCCCCEEEEEEECCCCCHHHHHHHHHCCEEEEEEECCCCCHHHHHHHHHHHCC
MRDGDSLRALIERERPDYIVPEIEAIATQTLLQLESDGQRVVPCARAVQLTMNREGIRRL
CCCCHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHCCCCEEECHHHHHHHHCCHHHHHHH
AAETLALPTSPYRFADSIEAFRQAVTEIGYPCFAKPVMSSSGKGQSILRNDDDVAPAWQY
HHHHHCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCHHHCCCCCCCHHHCCCCCCCHHHHH
ARQGARGDADSVIVEGEVRFDFEITLLTVQASDGIHFCAPIGHRQQEGDYRESWQPQAMS
HHCCCCCCCCCEEEECEEEEEEEEEEEEEECCCCEEEECCCCCCCCCCCHHHCCCCCCCC
PAALARAQAIAASVVRALGGYGLFGVELFVRGDEVIFSEVSPRPHDTGLVTLVSQDLSEF
HHHHHHHHHHHHHHHHHHCCCCEEEEEEEEECCEEEEECCCCCCCCCCEEEHHHHHHHHH
ALHVRAFLGLPIGVIRQFGPCASAVLLPSLHSDDVTFANLSQALRPATQLRLFGKPHIDG
HHHHHHHHCCCHHHHHHCCCHHHHHHCCCCCCCCCHHHHHHHHHCCHHHEEECCCCCHHH
VRRLGVALAWGESTETARQRARDSAASVVIRP
HHHHCEEEEECCCHHHHHHHHHHHHCCEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Formate; 5'-Phospho-Ribosylglycinamide; acetate; ATP [C]

Specific reaction: Formate + ATP + 5'-Phospho-Ribosylglycinamide = 5'-Phosphoribosyl-N-Formylglycinamide + ADP + Pyrophosphate. acetate + ATP = acetylphosphate + ADP [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA