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The map label for this gene is phsA [H]

Identifier: 238919750

GI number: 238919750

Start: 1789737

End: 1792013

Strand: Direct

Name: phsA [H]

Synonym: NT01EI_1854

Alternate gene names: 238919750

Gene position: 1789737-1792013 (Clockwise)

Preceding gene: 238919749

Following gene: 238919751

Centisome position: 46.95

GC content: 63.11

Gene sequence:

>2277_bases
ATGAGTATCAGTCGTCGTTATTTTCTTAAAGGCATTGGAGTCGGTTGTAGTGGATGTGCCCTCAGCAGTTTTCCGCCTGG
GGCGCTGGCGTTGAATCGACCCGATGAAATAAAAGGTAAGGCCAGGCTGACGCCCAGTCTGTGTGAGATGTGCTCTTTCC
GCTGCCCGATACAGGCGCAGGTGGTGAATAATAAAACGGTGTTTATCCAGGGTAATCCGCAGGCGGCGCATCAGGGCACC
CGGATCTGCGCCCGCGGCGGCAGCGGGGTGAGCCTGGTGAACGATCCGCAGCGTATCGTCAGGCCGATGAAGCGCCGCGG
CCCACGCGGCGCCGGGGAGTGGCAGACGATCAGTTGGCAGCAGGCCTACCGTGAGATCGCTGAAAAGATGAATGCCATCA
AGGCGCAGCACGGCGCGCAAAGCCTGGCCTTTTCCTCCAAGTCCGGATCCCTCTCAGGGCACCTGTTCCATTTGGCCTCC
GCCTATGGCTCTCCGAATACCTTCACCCATGCCTCGACCTGTCCGGCGGGTAAGTCGATCGCCGCCAAGGTGATGATGGG
CGGCGATCTGGCGATGGACATCGTCAATACCCGCTATATGGTGGCTTTCGGTCATAATCTGTATGAGGGGATCGAGGTTG
CTGATACGCATGAAATGATGATGGCGCAGGAGCGGGGCGCCAAGGTGGTCAGCTTCGATCCGCGGCTGTCGGTCTTCTCC
AGCAAGGCCGATGAATGGCATGCGATCCGCCCCGGCGGCGACCTGCCGGTGCTGATGGCGATGTGTCACGTGATGATCGC
CGAGCGCCTGTACGATGCGGCCTTTGTCGATCGCTATACCCTGGGATTCGATCGGCTGGCGGAGGCCGTCAGTGAGACTA
CGCCGACGTGGGCGCAGCAACATGCTGATATCCCGGCGCAGGTCATCGTACGGGTAACCCGTGAGCTGGCTGCCAGTGCG
CCCCACGCCATTGTCAATCCGGGCCACCGTGCGACCTATTCGCAGGAAGAGGTCGATATGCGGCGGATGATCTTCACCCT
GAATGCCCTGCTGGGTAACGTGGAGCGTCAGGGGGGGCTGTATCAGAAGAAGGGGGCCTCCGTCTATAACACGCTGGCCG
GTGAGACGGTGGCGCCGACGCTGGCCAAACCCGGCGTAAAGGGGATGCCATCCATCCAGGCGCCGCGCATTGACGCAACG
GCGCCGCAGTTTACCTATATCACCGCCAGCGGCGGCGTGGTGCAAAGCATTATCGATGCCGCGCTGACGCAGACGCCTTA
TCCGCTGAAGGGGTGGATTATGTCGCGTCATAATCCGTTTCAGACCGTCACCTGTCGGCCCGACCTGGTCAAAACCGTCG
AGAGGCTGGAGCTGGTGGTGAGCTGCGATGTGTATCTGAGCGAAAGCGCCGCCTATGCCGACTATCTGCTGCCGGAGTGC
ACCTATCTGGAGCGCGATGAGGAGGTCAGCGATGTGTCCGGCCTCAACCCAGCCTATGCGTTGCGTCAGCAGGTGGTGGA
GACCATCGGCGAAGCGCGGCCCAGCTGGCAGATCTGGAAGGAGCTGGGGGAGGCGCTGGGGCTGGGCCAGTACTACCCCT
GGGAGACGATGGCGACGCGCCAGCTGTTTCAGCTGGGTGGCGATCGGGCGCTGTACGGCGCGCTGCGCGAAAAGGGATAT
CTCAGCTGGGGGGTGCCGCTGCTGCTGCGCGAGCCGGCTTCGGTTGAGCGGTTTGTCGAGCGCTACCCTCAGGCGGCGCA
GCGGGATAGCGACGGCACGCTGGGCGGGGCCCTGCGCTTTAAGTCGCCAAGCGGACGTATCGAGCTCTATTCGGCGGCGC
TGGAGGCGCTCTTGCCGGGATATGGCGCCCCGCGCTTTCGCAACCTGCCCCTTAAGCAGCCGGATCAGCTCTATTTCATT
CAGGGTAAGGTGGCGGTGCACACCAACGGTGCGACGCAGTATGTGCCGCTGTTGAGCGAGCTGATGTGGGACAACGCAGT
GTGGGTGCATCCCCAGACGGCACAGGCGCGCGGTATCCGCAGCGGCGATACGGTGTGGCTGGAGAACCCGACCGGCCGGG
AGAAGGGACGGGCGCTGGTGACGCTGGCGATCCGCCCCGATACCCTGTTTGTCTATATGGGGTTTGGCGCCAAGGCCGGT
GCCCGTACGACGGCCAGCCGCCACGGGATCCACTGCGGCAATCTGCTGCCCCATGCAACCAGCCCGGTGTCCGGCACCGT
GGTCCACACCGCCGGGGTTACCCTGCGCCGCGCCTGA

Upstream 100 bases:

>100_bases
CGGTAAAATATTTTACCGCGTGCTGCTGATTTGCTGATGAGCTCAAGTAATGAAGATGTTTTCTCCCTATAATCATTTTG
TATAATAAAAAGGAGATTAT

Downstream 100 bases:

>100_bases
AGGAGCCGTCGATGAATCAGAACAACAACCACTATGTCATGCTACATGACGAGAAACGCTGCATTGGCTGCCAGGCCTGT
ACCGTCGCCTGCCGGGTGCT

Product: thiosulfate reductase

Products: Reduced form of N-Oxide compounds [C]

Alternate protein names: NA

Number of amino acids: Translated: 758; Mature: 757

Protein sequence:

>758_residues
MSISRRYFLKGIGVGCSGCALSSFPPGALALNRPDEIKGKARLTPSLCEMCSFRCPIQAQVVNNKTVFIQGNPQAAHQGT
RICARGGSGVSLVNDPQRIVRPMKRRGPRGAGEWQTISWQQAYREIAEKMNAIKAQHGAQSLAFSSKSGSLSGHLFHLAS
AYGSPNTFTHASTCPAGKSIAAKVMMGGDLAMDIVNTRYMVAFGHNLYEGIEVADTHEMMMAQERGAKVVSFDPRLSVFS
SKADEWHAIRPGGDLPVLMAMCHVMIAERLYDAAFVDRYTLGFDRLAEAVSETTPTWAQQHADIPAQVIVRVTRELAASA
PHAIVNPGHRATYSQEEVDMRRMIFTLNALLGNVERQGGLYQKKGASVYNTLAGETVAPTLAKPGVKGMPSIQAPRIDAT
APQFTYITASGGVVQSIIDAALTQTPYPLKGWIMSRHNPFQTVTCRPDLVKTVERLELVVSCDVYLSESAAYADYLLPEC
TYLERDEEVSDVSGLNPAYALRQQVVETIGEARPSWQIWKELGEALGLGQYYPWETMATRQLFQLGGDRALYGALREKGY
LSWGVPLLLREPASVERFVERYPQAAQRDSDGTLGGALRFKSPSGRIELYSAALEALLPGYGAPRFRNLPLKQPDQLYFI
QGKVAVHTNGATQYVPLLSELMWDNAVWVHPQTAQARGIRSGDTVWLENPTGREKGRALVTLAIRPDTLFVYMGFGAKAG
ARTTASRHGIHCGNLLPHATSPVSGTVVHTAGVTLRRA

Sequences:

>Translated_758_residues
MSISRRYFLKGIGVGCSGCALSSFPPGALALNRPDEIKGKARLTPSLCEMCSFRCPIQAQVVNNKTVFIQGNPQAAHQGT
RICARGGSGVSLVNDPQRIVRPMKRRGPRGAGEWQTISWQQAYREIAEKMNAIKAQHGAQSLAFSSKSGSLSGHLFHLAS
AYGSPNTFTHASTCPAGKSIAAKVMMGGDLAMDIVNTRYMVAFGHNLYEGIEVADTHEMMMAQERGAKVVSFDPRLSVFS
SKADEWHAIRPGGDLPVLMAMCHVMIAERLYDAAFVDRYTLGFDRLAEAVSETTPTWAQQHADIPAQVIVRVTRELAASA
PHAIVNPGHRATYSQEEVDMRRMIFTLNALLGNVERQGGLYQKKGASVYNTLAGETVAPTLAKPGVKGMPSIQAPRIDAT
APQFTYITASGGVVQSIIDAALTQTPYPLKGWIMSRHNPFQTVTCRPDLVKTVERLELVVSCDVYLSESAAYADYLLPEC
TYLERDEEVSDVSGLNPAYALRQQVVETIGEARPSWQIWKELGEALGLGQYYPWETMATRQLFQLGGDRALYGALREKGY
LSWGVPLLLREPASVERFVERYPQAAQRDSDGTLGGALRFKSPSGRIELYSAALEALLPGYGAPRFRNLPLKQPDQLYFI
QGKVAVHTNGATQYVPLLSELMWDNAVWVHPQTAQARGIRSGDTVWLENPTGREKGRALVTLAIRPDTLFVYMGFGAKAG
ARTTASRHGIHCGNLLPHATSPVSGTVVHTAGVTLRRA
>Mature_757_residues
SISRRYFLKGIGVGCSGCALSSFPPGALALNRPDEIKGKARLTPSLCEMCSFRCPIQAQVVNNKTVFIQGNPQAAHQGTR
ICARGGSGVSLVNDPQRIVRPMKRRGPRGAGEWQTISWQQAYREIAEKMNAIKAQHGAQSLAFSSKSGSLSGHLFHLASA
YGSPNTFTHASTCPAGKSIAAKVMMGGDLAMDIVNTRYMVAFGHNLYEGIEVADTHEMMMAQERGAKVVSFDPRLSVFSS
KADEWHAIRPGGDLPVLMAMCHVMIAERLYDAAFVDRYTLGFDRLAEAVSETTPTWAQQHADIPAQVIVRVTRELAASAP
HAIVNPGHRATYSQEEVDMRRMIFTLNALLGNVERQGGLYQKKGASVYNTLAGETVAPTLAKPGVKGMPSIQAPRIDATA
PQFTYITASGGVVQSIIDAALTQTPYPLKGWIMSRHNPFQTVTCRPDLVKTVERLELVVSCDVYLSESAAYADYLLPECT
YLERDEEVSDVSGLNPAYALRQQVVETIGEARPSWQIWKELGEALGLGQYYPWETMATRQLFQLGGDRALYGALREKGYL
SWGVPLLLREPASVERFVERYPQAAQRDSDGTLGGALRFKSPSGRIELYSAALEALLPGYGAPRFRNLPLKQPDQLYFIQ
GKVAVHTNGATQYVPLLSELMWDNAVWVHPQTAQARGIRSGDTVWLENPTGREKGRALVTLAIRPDTLFVYMGFGAKAGA
RTTASRHGIHCGNLLPHATSPVSGTVVHTAGVTLRRA

Specific function: Oxidoreductase which produces hydrogen sulfide from thiosulfate (Potential) [H]

COG id: COG0243

COG function: function code C; Anaerobic dehydrogenases, typically selenocysteine-containing

Gene ontology:

Cell location: Cytoplasm Face Of The Membrane [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the prokaryotic molybdopterin-containing oxidoreductase family [H]

Homologues:

Organism=Escherichia coli, GI87081797, Length=801, Percent_Identity=26.9662921348315, Blast_Score=189, Evalue=6e-49,
Organism=Escherichia coli, GI171474008, Length=832, Percent_Identity=25.7211538461538, Blast_Score=184, Evalue=2e-47,
Organism=Escherichia coli, GI1787870, Length=840, Percent_Identity=26.6666666666667, Blast_Score=182, Evalue=5e-47,
Organism=Escherichia coli, GI3868721, Length=740, Percent_Identity=24.3243243243243, Blast_Score=139, Evalue=5e-34,
Organism=Escherichia coli, GI1787231, Length=696, Percent_Identity=22.9885057471264, Blast_Score=103, Evalue=3e-23,
Organism=Escherichia coli, GI87081994, Length=804, Percent_Identity=22.636815920398, Blast_Score=95, Evalue=2e-20,
Organism=Escherichia coli, GI3868720, Length=309, Percent_Identity=25.5663430420712, Blast_Score=80, Evalue=7e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR009010
- InterPro:   IPR006657
- InterPro:   IPR006656
- InterPro:   IPR006963
- InterPro:   IPR006655
- InterPro:   IPR006311 [H]

Pfam domain/function: PF04879 Molybdop_Fe4S4; PF00384 Molybdopterin; PF01568 Molydop_binding [H]

EC number: 1.8.99.- [C]

Molecular weight: Translated: 82751; Mature: 82619

Theoretical pI: Translated: 8.98; Mature: 8.98

Prosite motif: PS00551 MOLYBDOPTERIN_PROK_1 ; PS00490 MOLYBDOPTERIN_PROK_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSISRRYFLKGIGVGCSGCALSSFPPGALALNRPDEIKGKARLTPSLCEMCSFRCPIQAQ
CCCCHHHHHHCCCCCCCCCCCCCCCCCCEECCCCHHCCCCCCCCHHHHHHHCCCCCEEEE
VVNNKTVFIQGNPQAAHQGTRICARGGSGVSLVNDPQRIVRPMKRRGPRGAGEWQTISWQ
EECCEEEEEECCCHHHHCCCEEEECCCCCCCCCCCHHHHHHHHHHCCCCCCCCCCEECHH
QAYREIAEKMNAIKAQHGAQSLAFSSKSGSLSGHLFHLASAYGSPNTFTHASTCPAGKSI
HHHHHHHHHHHHHHHHCCHHHHEECCCCCCCCHHHHHHHHHCCCCCCCCCCCCCCCCCHH
AAKVMMGGDLAMDIVNTRYMVAFGHNLYEGIEVADTHEMMMAQERGAKVVSFDPRLSVFS
HHHEEECCCHHHHHHCCEEEEEECCHHHCCCCCCCHHHHHHHHHCCCEEEEECCCHHHHH
SKADEWHAIRPGGDLPVLMAMCHVMIAERLYDAAFVDRYTLGFDRLAEAVSETTPTWAQQ
CCCCCCEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCHHHHH
HADIPAQVIVRVTRELAASAPHAIVNPGHRATYSQEEVDMRRMIFTLNALLGNVERQGGL
HCCCHHHHHHHHHHHHHHCCCCHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCC
YQKKGASVYNTLAGETVAPTLAKPGVKGMPSIQAPRIDATAPQFTYITASGGVVQSIIDA
CHHCCCHHHHHHCCCCCCCHHCCCCCCCCCCCCCCCCCCCCCCEEEEEECCCHHHHHHHH
ALTQTPYPLKGWIMSRHNPFQTVTCRPDLVKTVERLELVVSCDVYLSESAAYADYLLPEC
HHHCCCCCHHHHEECCCCCCEEEECCHHHHHHHHHHHEEEEECEEECCCHHHHHHHCCCC
TYLERDEEVSDVSGLNPAYALRQQVVETIGEARPSWQIWKELGEALGLGQYYPWETMATR
CCCCCCCCHHHHCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCCCCHHHHHH
QLFQLGGDRALYGALREKGYLSWGVPLLLREPASVERFVERYPQAAQRDSDGTLGGALRF
HHHHCCCCHHHHHHHHHCCCEECCCCEEEECCHHHHHHHHHCCHHHCCCCCCCCCCEEEE
KSPSGRIELYSAALEALLPGYGAPRFRNLPLKQPDQLYFIQGKVAVHTNGATQYVPLLSE
CCCCCCEEHHHHHHHHHHCCCCCCCCCCCCCCCCCCEEEEEEEEEEEECCCHHHHHHHHH
LMWDNAVWVHPQTAQARGIRSGDTVWLENPTGREKGRALVTLAIRPDTLFVYMGFGAKAG
HHHCCEEEECCCCHHHCCCCCCCEEEEECCCCCCCCCEEEEEEECCCEEEEEECCCCCCC
ARTTASRHGIHCGNLLPHATSPVSGTVVHTAGVTLRRA
CCCCHHHCCCCCCCCCCCCCCCCCCEEEEECCEEEECC
>Mature Secondary Structure 
SISRRYFLKGIGVGCSGCALSSFPPGALALNRPDEIKGKARLTPSLCEMCSFRCPIQAQ
CCCHHHHHHCCCCCCCCCCCCCCCCCCEECCCCHHCCCCCCCCHHHHHHHCCCCCEEEE
VVNNKTVFIQGNPQAAHQGTRICARGGSGVSLVNDPQRIVRPMKRRGPRGAGEWQTISWQ
EECCEEEEEECCCHHHHCCCEEEECCCCCCCCCCCHHHHHHHHHHCCCCCCCCCCEECHH
QAYREIAEKMNAIKAQHGAQSLAFSSKSGSLSGHLFHLASAYGSPNTFTHASTCPAGKSI
HHHHHHHHHHHHHHHHCCHHHHEECCCCCCCCHHHHHHHHHCCCCCCCCCCCCCCCCCHH
AAKVMMGGDLAMDIVNTRYMVAFGHNLYEGIEVADTHEMMMAQERGAKVVSFDPRLSVFS
HHHEEECCCHHHHHHCCEEEEEECCHHHCCCCCCCHHHHHHHHHCCCEEEEECCCHHHHH
SKADEWHAIRPGGDLPVLMAMCHVMIAERLYDAAFVDRYTLGFDRLAEAVSETTPTWAQQ
CCCCCCEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCHHHHH
HADIPAQVIVRVTRELAASAPHAIVNPGHRATYSQEEVDMRRMIFTLNALLGNVERQGGL
HCCCHHHHHHHHHHHHHHCCCCHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCC
YQKKGASVYNTLAGETVAPTLAKPGVKGMPSIQAPRIDATAPQFTYITASGGVVQSIIDA
CHHCCCHHHHHHCCCCCCCHHCCCCCCCCCCCCCCCCCCCCCCEEEEEECCCHHHHHHHH
ALTQTPYPLKGWIMSRHNPFQTVTCRPDLVKTVERLELVVSCDVYLSESAAYADYLLPEC
HHHCCCCCHHHHEECCCCCCEEEECCHHHHHHHHHHHEEEEECEEECCCHHHHHHHCCCC
TYLERDEEVSDVSGLNPAYALRQQVVETIGEARPSWQIWKELGEALGLGQYYPWETMATR
CCCCCCCCHHHHCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCCCCHHHHHH
QLFQLGGDRALYGALREKGYLSWGVPLLLREPASVERFVERYPQAAQRDSDGTLGGALRF
HHHHCCCCHHHHHHHHHCCCEECCCCEEEECCHHHHHHHHHCCHHHCCCCCCCCCCEEEE
KSPSGRIELYSAALEALLPGYGAPRFRNLPLKQPDQLYFIQGKVAVHTNGATQYVPLLSE
CCCCCCEEHHHHHHHHHHCCCCCCCCCCCCCCCCCCEEEEEEEEEEEECCCHHHHHHHHH
LMWDNAVWVHPQTAQARGIRSGDTVWLENPTGREKGRALVTLAIRPDTLFVYMGFGAKAG
HHHCCEEEECCCCHHHCCCCCCCEEEEECCCCCCCCCEEEEEEECCCEEEEEECCCCCCC
ARTTASRHGIHCGNLLPHATSPVSGTVVHTAGVTLRRA
CCCCHHHCCCCCCCCCCCCCCCCCCEEEEECCEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: May Bind 4Fe-4S Cluster. [C]

Metal ions: Fe; Mo [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: N-Oxide; Sulfoxide Compounds Including Trimethylamine N-Oxide. [C]

Specific reaction: Reduces Various N-Oxide And Sulfoxide Compounds Including Trimethylamine N-Oxide. [C]

General reaction: Oxidoreductases [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7751291; 11677609; 7737516 [H]