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The map label for this gene is potB [H]

Identifier: 238919714

GI number: 238919714

Start: 1753666

End: 1754532

Strand: Reverse

Name: potB [H]

Synonym: NT01EI_1817

Alternate gene names: 238919714

Gene position: 1754532-1753666 (Counterclockwise)

Preceding gene: 238919715

Following gene: 238919713

Centisome position: 46.02

GC content: 60.44

Gene sequence:

>867_bases
ATGAAGAAATCGCGTAAACTGTTTCAAAATGTCGTGATCGCGCTGGTGGTAGGCTGGCTGTCGCTGTTCGTCTTCCTGCC
TAACCTGATGATCATCGGCACCAGCTTTCTGACCCGCGATGACGCCAGCCTGGTACAGATGGTGTTCACCTGGGGCAACT
ACGCCCGGCTGTTCGATCCGCTGTATGCCGAGGTGCTGCTGCACTCGCTCAACATGGCGCTGATCGCCACCCTGTGCTGT
CTGGTCATCGGCTATCCCTTTGCCTTTATTCTGGCGCGGCTGCCCCACCGGGTACGCCCGCTGCTGCTGTTTCTGCTGAT
CGTCCCGTTCTGGACCAACTCGCTGATCCGGATCTACGGGCTGAAGCTGTTCCTCAGCACCCGCGGCTACCTCAACGACT
TCCTGCTCTGGGTCGGCATCATCGATCAGCCGCTGCGGCTGATGTACACCTCCACGGCGGTGGTGCTGGGGCTGATTTAC
ATTCTGCTGCCCTTTATGGTGATGCCGCTCTACTCCAGCATTGAAAAGCTGGATCGCTCGGTGCTGGAGGCGGCGCGCGA
CCTGGGCGCCAACCGGCTACAGACCTTCATGCGCATCATCGTCCCGCTGACCATGCCGGGGATCGTCGCCGGCTGCCTGC
TGGTGCTGCTGCCGTCGCTGGGCATGTTCTACATCGCCGACCTGCTGGGCGGCGCCAAAAACCTGCTGATCGGCAACGTC
ATCAAGAGCCAGTTCCTCAATATCCGCGACTGGCCGTTTGGCGCCGCCACCAGCATCTGCCTGACGCTGGTGATGGGGGT
GATGCTGCTGATCTACTACCGGGTCGGTAAGCTGCTCAACCGTAAGGCCAGCGATTTGGCAGACTGA

Upstream 100 bases:

>100_bases
GTCAGCGAGTTTTTCAACGAGGACGATCCCGACGTAGACCATTCGCTCGATCAGAAGGTCGCAGTCACCTGGGTCGAAAG
CTGGGAGGTGGTGCTGCCTG

Downstream 100 bases:

>100_bases
CGGACAGGCGCGGTGTTGGCGTCGTACCGAGAGGTCAATATTTAATATCCATTTGTTTTATATTATAAAATTTTGTCGAA
TGGTTTGGAGAAGATAACAA

Product: spermidine/putrescine ABC transporter membrane protein

Products: phosphate; putrescine [Cytoplasm]; ADP; spermidine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 288; Mature: 288

Protein sequence:

>288_residues
MKKSRKLFQNVVIALVVGWLSLFVFLPNLMIIGTSFLTRDDASLVQMVFTWGNYARLFDPLYAEVLLHSLNMALIATLCC
LVIGYPFAFILARLPHRVRPLLLFLLIVPFWTNSLIRIYGLKLFLSTRGYLNDFLLWVGIIDQPLRLMYTSTAVVLGLIY
ILLPFMVMPLYSSIEKLDRSVLEAARDLGANRLQTFMRIIVPLTMPGIVAGCLLVLLPSLGMFYIADLLGGAKNLLIGNV
IKSQFLNIRDWPFGAATSICLTLVMGVMLLIYYRVGKLLNRKASDLAD

Sequences:

>Translated_288_residues
MKKSRKLFQNVVIALVVGWLSLFVFLPNLMIIGTSFLTRDDASLVQMVFTWGNYARLFDPLYAEVLLHSLNMALIATLCC
LVIGYPFAFILARLPHRVRPLLLFLLIVPFWTNSLIRIYGLKLFLSTRGYLNDFLLWVGIIDQPLRLMYTSTAVVLGLIY
ILLPFMVMPLYSSIEKLDRSVLEAARDLGANRLQTFMRIIVPLTMPGIVAGCLLVLLPSLGMFYIADLLGGAKNLLIGNV
IKSQFLNIRDWPFGAATSICLTLVMGVMLLIYYRVGKLLNRKASDLAD
>Mature_288_residues
MKKSRKLFQNVVIALVVGWLSLFVFLPNLMIIGTSFLTRDDASLVQMVFTWGNYARLFDPLYAEVLLHSLNMALIATLCC
LVIGYPFAFILARLPHRVRPLLLFLLIVPFWTNSLIRIYGLKLFLSTRGYLNDFLLWVGIIDQPLRLMYTSTAVVLGLIY
ILLPFMVMPLYSSIEKLDRSVLEAARDLGANRLQTFMRIIVPLTMPGIVAGCLLVLLPSLGMFYIADLLGGAKNLLIGNV
IKSQFLNIRDWPFGAATSICLTLVMGVMLLIYYRVGKLLNRKASDLAD

Specific function: Required for the activity of the bacterial periplasmic transport system of putrescine and spermidine [H]

COG id: COG1176

COG function: function code E; ABC-type spermidine/putrescine transport system, permease component I

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transmembrane type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1787369, Length=273, Percent_Identity=78.3882783882784, Blast_Score=450, Evalue=1e-128,
Organism=Escherichia coli, GI1787080, Length=224, Percent_Identity=45.9821428571429, Blast_Score=211, Evalue=6e-56,
Organism=Escherichia coli, GI1787713, Length=275, Percent_Identity=28.3636363636364, Blast_Score=114, Evalue=6e-27,
Organism=Escherichia coli, GI1788764, Length=226, Percent_Identity=26.9911504424779, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI1786980, Length=239, Percent_Identity=28.4518828451883, Blast_Score=65, Evalue=7e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000515 [H]

Pfam domain/function: PF00528 BPD_transp_1 [H]

EC number: NA

Molecular weight: Translated: 32428; Mature: 32428

Theoretical pI: Translated: 10.00; Mature: 10.00

Prosite motif: PS50928 ABC_TM1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
4.2 %Met     (Translated Protein)
5.6 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
4.2 %Met     (Mature Protein)
5.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHCCCC
>Mature Secondary Structure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HHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: putrescine [Periplasm]; H2O; ATP; spermidine [Periplasm] [C]

Specific reaction: ATP + putrescine [Periplasm] + H2O = ADP + phosphate + putrescine [Cytoplasm] ATP + spermidine [Periplasm] + H2O = ADP + phosphate + spermidine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 11677608; 12644504 [H]