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The map label for this gene is trpB

Identifier: 238919589

GI number: 238919589

Start: 1648056

End: 1649249

Strand: Reverse

Name: trpB

Synonym: NT01EI_1688

Alternate gene names: 238919589

Gene position: 1649249-1648056 (Counterclockwise)

Preceding gene: 238919590

Following gene: 238919588

Centisome position: 43.26

GC content: 62.73

Gene sequence:

>1194_bases
ATGAGCTTACTGAATCCCTATTTCGGCGAATTTGGCGGCAGGTTTGTGCCGCAAATTCTGATCCCGGCCCTGAACCAGCT
GGAGGCCGCCTTCGTCGACGCCCGCGACGATCCCACCTTTCAGACCCAGTTCAATGACCTGCTGCACAACTATGCCGGGC
GACCGACGGCGTTGACCCTCTGCCGCAATCTCACCGCCGGTACCCGCACCCGCCTGTACCTAAAGCGTGAGGATCTGCTG
CACGGCGGCGCGCACAAAACCAATCAGGTACTTGGCCAGGCGCTGCTGGCCAGGCGCATGGGTAAGACCGAGATCATCGC
GGAAACCGGCGCGGGTCAGCACGGCGTCGCAACGGCGCTGGCCTGTGCCTTGCTGGGTCTGAAATGCCGGGTCTACATGG
GGGCCAAAGACGTTGCCCGCCAGTCTCCCAATGTGTTCCGTATGCGGCTGATGGGCGCCGAGGTGATCCCGGTGCACAGC
GGCTCGGCCACCCTAAAAGATGCCTGCAATGAGGCGCTGCGCGACTGGTCCGCCAACTATGACCAGGCCCACTATCTGTT
GGGCACCGCCGCCGGCCCCCATCCCTATCCGACCATCGTGCGCGAATTTCAGCGGATGATCGGCGCGGAGACGCGCCGTC
AGATCCTGCAGGCCGAAGGCCGCCTGCCCGATGCGGTATTGGCCTGCGTCGGCGGCGGCTCCAACGCCATCGGGATGTTC
GCCGACTTTATCGATGATGCGTCGGTTCAGCTTATCGGCGTCGAACCGGCTGGGCTGGGCATTGAGACCGGGCAGCACGG
CGCGCCGCTCAGTCACGGCCGCGTTGGCATTTACTTCGGCATGCGCTCTCCCATGATGCAGACCGCCGAGGGGCAGATTG
AGGAGTCCTACTCCCTTTCCGCCGGGCTGGATTTTCCCTCGGTAGGCCCCCAGCACGCCCACCTGAACAGCATTGGCCGG
GCCGACTATGTCTCCGCGACCGACGACGAAGCGTTGGCCGCCTTTATGCAGCTCTCGCGCCAGGAGGGCATTATTCCGGC
GTTAGAGTCCGCCCATGCGTTGGCCCACGCCCTGAAGCTCATTCATCAGCAGCCAGAAAAAGAGCAGCTGCTGGTGGTGA
ATCTTTCCGGGCGCGGTGACAAGGATATTTTTACCGTTCACGACATTCTGCAGTCCCGGGGAGAAATACAATGA

Upstream 100 bases:

>100_bases
CCTCTCCCGGGCGTAAAGACCCCGGCAAACTCCGTGCGGCCTTCCAGGCGCTGCTCGCCTGAGACGCTACCACCGTTTTT
ATGACTGACAGGAAGATGAC

Downstream 100 bases:

>100_bases
CACGCTACCAGACGCTATTTACCCATCTACAGCGCCGCCAGGAAGGCGCGCTGGTCCCCTTCGTCACACTGGGCGACCCT
ACGCCGTCGCTGTCGCTGCA

Product: tryptophan synthase subunit beta

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 397; Mature: 396

Protein sequence:

>397_residues
MSLLNPYFGEFGGRFVPQILIPALNQLEAAFVDARDDPTFQTQFNDLLHNYAGRPTALTLCRNLTAGTRTRLYLKREDLL
HGGAHKTNQVLGQALLARRMGKTEIIAETGAGQHGVATALACALLGLKCRVYMGAKDVARQSPNVFRMRLMGAEVIPVHS
GSATLKDACNEALRDWSANYDQAHYLLGTAAGPHPYPTIVREFQRMIGAETRRQILQAEGRLPDAVLACVGGGSNAIGMF
ADFIDDASVQLIGVEPAGLGIETGQHGAPLSHGRVGIYFGMRSPMMQTAEGQIEESYSLSAGLDFPSVGPQHAHLNSIGR
ADYVSATDDEALAAFMQLSRQEGIIPALESAHALAHALKLIHQQPEKEQLLVVNLSGRGDKDIFTVHDILQSRGEIQ

Sequences:

>Translated_397_residues
MSLLNPYFGEFGGRFVPQILIPALNQLEAAFVDARDDPTFQTQFNDLLHNYAGRPTALTLCRNLTAGTRTRLYLKREDLL
HGGAHKTNQVLGQALLARRMGKTEIIAETGAGQHGVATALACALLGLKCRVYMGAKDVARQSPNVFRMRLMGAEVIPVHS
GSATLKDACNEALRDWSANYDQAHYLLGTAAGPHPYPTIVREFQRMIGAETRRQILQAEGRLPDAVLACVGGGSNAIGMF
ADFIDDASVQLIGVEPAGLGIETGQHGAPLSHGRVGIYFGMRSPMMQTAEGQIEESYSLSAGLDFPSVGPQHAHLNSIGR
ADYVSATDDEALAAFMQLSRQEGIIPALESAHALAHALKLIHQQPEKEQLLVVNLSGRGDKDIFTVHDILQSRGEIQ
>Mature_396_residues
SLLNPYFGEFGGRFVPQILIPALNQLEAAFVDARDDPTFQTQFNDLLHNYAGRPTALTLCRNLTAGTRTRLYLKREDLLH
GGAHKTNQVLGQALLARRMGKTEIIAETGAGQHGVATALACALLGLKCRVYMGAKDVARQSPNVFRMRLMGAEVIPVHSG
SATLKDACNEALRDWSANYDQAHYLLGTAAGPHPYPTIVREFQRMIGAETRRQILQAEGRLPDAVLACVGGGSNAIGMFA
DFIDDASVQLIGVEPAGLGIETGQHGAPLSHGRVGIYFGMRSPMMQTAEGQIEESYSLSAGLDFPSVGPQHAHLNSIGRA
DYVSATDDEALAAFMQLSRQEGIIPALESAHALAHALKLIHQQPEKEQLLVVNLSGRGDKDIFTVHDILQSRGEIQ

Specific function: The beta subunit is responsible for the synthesis of L- tryptophan from indole and L-serine

COG id: COG0133

COG function: function code E; Tryptophan synthase beta chain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the trpB family

Homologues:

Organism=Escherichia coli, GI1787515, Length=395, Percent_Identity=83.5443037974684, Blast_Score=695, Evalue=0.0,
Organism=Saccharomyces cerevisiae, GI6321412, Length=389, Percent_Identity=51.6709511568123, Blast_Score=406, Evalue=1e-114,

Paralogues:

None

Copy number: 360 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 5,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): TRPB_EDWI9 (C5BDB7)

Other databases:

- EMBL:   CP001600
- RefSeq:   YP_002933104.1
- GeneID:   7958921
- GenomeReviews:   CP001600_GR
- KEGG:   eic:NT01EI_1688
- OMA:   LKREDLC
- ProtClustDB:   PRK04346
- HAMAP:   MF_00133
- InterPro:   IPR001926
- InterPro:   IPR006653
- InterPro:   IPR006654
- InterPro:   IPR023026
- TIGRFAMs:   TIGR00263

Pfam domain/function: PF00291 PALP; SSF53686 PyrdxlP-dep_enz_bsu

EC number: =4.2.1.20

Molecular weight: Translated: 42897; Mature: 42766

Theoretical pI: Translated: 6.56; Mature: 6.56

Prosite motif: PS00168 TRP_SYNTHASE_BETA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSLLNPYFGEFGGRFVPQILIPALNQLEAAFVDARDDPTFQTQFNDLLHNYAGRPTALTL
CCCCCCHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCCHHHHH
CRNLTAGTRTRLYLKREDLLHGGAHKTNQVLGQALLARRMGKTEIIAETGAGQHGVATAL
HHHCCCCCCEEEEEEHHHHHCCCCCHHHHHHHHHHHHHHCCCCEEEEECCCCCCHHHHHH
ACALLGLKCRVYMGAKDVARQSPNVFRMRLMGAEVIPVHSGSATLKDACNEALRDWSANY
HHHHHHHHHEEEECCHHHHHCCCCCEEEEECCCEEEEECCCCCHHHHHHHHHHHHHCCCC
DQAHYLLGTAAGPHPYPTIVREFQRMIGAETRRQILQAEGRLPDAVLACVGGGSNAIGMF
CCHHEEEEECCCCCCCHHHHHHHHHHHCHHHHHHHHHHCCCCCHHHHEEECCCCCHHHHH
ADFIDDASVQLIGVEPAGLGIETGQHGAPLSHGRVGIYFGMRSPMMQTAEGQIEESYSLS
HHHHCCCCEEEEEECCCCCCCCCCCCCCCCCCCCEEEEECCCCCHHHHHCCCHHHCCCCC
AGLDFPSVGPQHAHLNSIGRADYVSATDDEALAAFMQLSRQEGIIPALESAHALAHALKL
CCCCCCCCCCCHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHH
IHQQPEKEQLLVVNLSGRGDKDIFTVHDILQSRGEIQ
HHCCCCCCCEEEEEECCCCCCCHHHHHHHHHHCCCCC
>Mature Secondary Structure 
SLLNPYFGEFGGRFVPQILIPALNQLEAAFVDARDDPTFQTQFNDLLHNYAGRPTALTL
CCCCCHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCCHHHHH
CRNLTAGTRTRLYLKREDLLHGGAHKTNQVLGQALLARRMGKTEIIAETGAGQHGVATAL
HHHCCCCCCEEEEEEHHHHHCCCCCHHHHHHHHHHHHHHCCCCEEEEECCCCCCHHHHHH
ACALLGLKCRVYMGAKDVARQSPNVFRMRLMGAEVIPVHSGSATLKDACNEALRDWSANY
HHHHHHHHHEEEECCHHHHHCCCCCEEEEECCCEEEEECCCCCHHHHHHHHHHHHHCCCC
DQAHYLLGTAAGPHPYPTIVREFQRMIGAETRRQILQAEGRLPDAVLACVGGGSNAIGMF
CCHHEEEEECCCCCCCHHHHHHHHHHHCHHHHHHHHHHCCCCCHHHHEEECCCCCHHHHH
ADFIDDASVQLIGVEPAGLGIETGQHGAPLSHGRVGIYFGMRSPMMQTAEGQIEESYSLS
HHHHCCCCEEEEEECCCCCCCCCCCCCCCCCCCCEEEEECCCCCHHHHHCCCHHHCCCCC
AGLDFPSVGPQHAHLNSIGRADYVSATDDEALAAFMQLSRQEGIIPALESAHALAHALKL
CCCCCCCCCCCHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHH
IHQQPEKEQLLVVNLSGRGDKDIFTVHDILQSRGEIQ
HHCCCCCCCEEEEEECCCCCCCHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA