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The map label for this gene is trpA

Identifier: 238919588

GI number: 238919588

Start: 1647253

End: 1648059

Strand: Reverse

Name: trpA

Synonym: NT01EI_1687

Alternate gene names: 238919588

Gene position: 1648059-1647253 (Counterclockwise)

Preceding gene: 238919589

Following gene: 238919587

Centisome position: 43.23

GC content: 66.42

Gene sequence:

>807_bases
ATGACACGCTACCAGACGCTATTTACCCATCTACAGCGCCGCCAGGAAGGCGCGCTGGTCCCCTTCGTCACACTGGGCGA
CCCTACGCCGTCGCTGTCGCTGCAAATTATCGACGCGCTGGTGGAGTCCGGCGCCGATGCGCTGGAGCTGGGGATCCCCT
TCTCCGACCCGCTGGCCGACGGCCCGGTGATCCAGAGTGCGACTCTGCGCGCCCTGCAGGCGGGCGTCACCCCCGAGCGT
TGCTTTACGCTGCTGGCTACCCTGCGGGAAAAATATCCCCAGTTGCCCATTGGACTGCTGATGTACGCTAACCTGGTGGT
TAATCCAGGGATCGACACCTTTTACCGGCGCTGTGCCCAGGCCGGCATTGACTCGGTCTTGATTGCCGATGTCCCGCTGG
AGGAGTCCGCCCCCTTCCACGCGGCGGCGCAGCGGTACGGGGTCGCGCCGATCTACCTGTGTCCGCCCAACGCCGATGAT
GTACTGCTGCAGCAACTGGCCGCGCGCAGTCAGGGGTACGTTTACCTGCTGTCACGCGCCGGGGTCACCGGCAGCGATCG
CCCCTGCCTGCCGCCACTGCGCCATCTCACGACGCGGCTGGCAGCGCTGGGTGCCGCGCCGACGCTACAGGGATTCGGGA
TTCAGACACCCGATCAGGTACGCAGCGCGCTGGACGGTGGCGCCGCGGGCGCCATTTCCGGCTCGGCAGTGGTCGACCTG
ATCGCTCGCCATCTGGATACCCCGCCCCAGATGCTGCAGCAGCTGCGCGCGTTCATCCAAACCATGAAGGCCGCAACCCG
CGCCTGA

Upstream 100 bases:

>100_bases
AGCCAGAAAAAGAGCAGCTGCTGGTGGTGAATCTTTCCGGGCGCGGTGACAAGGATATTTTTACCGTTCACGACATTCTG
CAGTCCCGGGGAGAAATACA

Downstream 100 bases:

>100_bases
TGCCTCCGCGGCGGCCGCTCTCCCCGCCGCGGATTTTGACTTGCCCTCTGCTGACCTATTGATATGATTATTCATAGAGC
GGGACACCGTTCATGGCGGC

Product: tryptophan synthase, alpha subunit

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 268; Mature: 267

Protein sequence:

>268_residues
MTRYQTLFTHLQRRQEGALVPFVTLGDPTPSLSLQIIDALVESGADALELGIPFSDPLADGPVIQSATLRALQAGVTPER
CFTLLATLREKYPQLPIGLLMYANLVVNPGIDTFYRRCAQAGIDSVLIADVPLEESAPFHAAAQRYGVAPIYLCPPNADD
VLLQQLAARSQGYVYLLSRAGVTGSDRPCLPPLRHLTTRLAALGAAPTLQGFGIQTPDQVRSALDGGAAGAISGSAVVDL
IARHLDTPPQMLQQLRAFIQTMKAATRA

Sequences:

>Translated_268_residues
MTRYQTLFTHLQRRQEGALVPFVTLGDPTPSLSLQIIDALVESGADALELGIPFSDPLADGPVIQSATLRALQAGVTPER
CFTLLATLREKYPQLPIGLLMYANLVVNPGIDTFYRRCAQAGIDSVLIADVPLEESAPFHAAAQRYGVAPIYLCPPNADD
VLLQQLAARSQGYVYLLSRAGVTGSDRPCLPPLRHLTTRLAALGAAPTLQGFGIQTPDQVRSALDGGAAGAISGSAVVDL
IARHLDTPPQMLQQLRAFIQTMKAATRA
>Mature_267_residues
TRYQTLFTHLQRRQEGALVPFVTLGDPTPSLSLQIIDALVESGADALELGIPFSDPLADGPVIQSATLRALQAGVTPERC
FTLLATLREKYPQLPIGLLMYANLVVNPGIDTFYRRCAQAGIDSVLIADVPLEESAPFHAAAQRYGVAPIYLCPPNADDV
LLQQLAARSQGYVYLLSRAGVTGSDRPCLPPLRHLTTRLAALGAAPTLQGFGIQTPDQVRSALDGGAAGAISGSAVVDLI
ARHLDTPPQMLQQLRAFIQTMKAATRA

Specific function: The alpha subunit is responsible for the aldol cleavage of indoleglycerol phosphate to indole and glyceraldehyde 3- phosphate

COG id: COG0159

COG function: function code E; Tryptophan synthase alpha chain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the trpA family

Homologues:

Organism=Escherichia coli, GI1787514, Length=268, Percent_Identity=64.1791044776119, Blast_Score=346, Evalue=7e-97,
Organism=Saccharomyces cerevisiae, GI6321412, Length=234, Percent_Identity=33.3333333333333, Blast_Score=126, Evalue=4e-30,

Paralogues:

None

Copy number: 280 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 3,500 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): TRPA_EDWI9 (C5BDB6)

Other databases:

- EMBL:   CP001600
- RefSeq:   YP_002933103.1
- GeneID:   7958920
- GenomeReviews:   CP001600_GR
- KEGG:   eic:NT01EI_1687
- OMA:   NPIYIYG
- ProtClustDB:   CLSK2813133
- HAMAP:   MF_00131
- InterPro:   IPR013785
- InterPro:   IPR011060
- InterPro:   IPR018204
- InterPro:   IPR002028
- Gene3D:   G3DSA:3.20.20.70
- TIGRFAMs:   TIGR00262

Pfam domain/function: PF00290 Trp_syntA; SSF51366 RibP_bind_barrel

EC number: =4.2.1.20

Molecular weight: Translated: 28607; Mature: 28476

Theoretical pI: Translated: 6.50; Mature: 6.50

Prosite motif: PS00167 TRP_SYNTHASE_ALPHA

Important sites: ACT_SITE 49-49 ACT_SITE 60-60

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTRYQTLFTHLQRRQEGALVPFVTLGDPTPSLSLQIIDALVESGADALELGIPFSDPLAD
CCHHHHHHHHHHHHHCCCEEEEEECCCCCCCHHHHHHHHHHHCCCCEEEECCCCCCCCCC
GPVIQSATLRALQAGVTPERCFTLLATLREKYPQLPIGLLMYANLVVNPGIDTFYRRCAQ
CCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCHHHHHHHHHH
AGIDSVLIADVPLEESAPFHAAAQRYGVAPIYLCPPNADDVLLQQLAARSQGYVYLLSRA
HCCCEEEEECCCCCCCCCHHHHHHHCCCCEEEECCCCCHHHHHHHHHHCCCCEEEEEEEC
GVTGSDRPCLPPLRHLTTRLAALGAAPTLQGFGIQTPDQVRSALDGGAAGAISGSAVVDL
CCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHCCCCCCCCCHHHHHHH
IARHLDTPPQMLQQLRAFIQTMKAATRA
HHHHCCCCHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
TRYQTLFTHLQRRQEGALVPFVTLGDPTPSLSLQIIDALVESGADALELGIPFSDPLAD
CHHHHHHHHHHHHHCCCEEEEEECCCCCCCHHHHHHHHHHHCCCCEEEECCCCCCCCCC
GPVIQSATLRALQAGVTPERCFTLLATLREKYPQLPIGLLMYANLVVNPGIDTFYRRCAQ
CCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCHHHHHHHHHH
AGIDSVLIADVPLEESAPFHAAAQRYGVAPIYLCPPNADDVLLQQLAARSQGYVYLLSRA
HCCCEEEEECCCCCCCCCHHHHHHHCCCCEEEECCCCCHHHHHHHHHHCCCCEEEEEEEC
GVTGSDRPCLPPLRHLTTRLAALGAAPTLQGFGIQTPDQVRSALDGGAAGAISGSAVVDL
CCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHCCCCCCCCCHHHHHHH
IARHLDTPPQMLQQLRAFIQTMKAATRA
HHHHCCCCHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA