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The map label for this gene is cls

Identifier: 238919575

GI number: 238919575

Start: 1634011

End: 1635471

Strand: Reverse

Name: cls

Synonym: NT01EI_1674

Alternate gene names: 238919575

Gene position: 1635471-1634011 (Counterclockwise)

Preceding gene: 238919578

Following gene: 238919574

Centisome position: 42.9

GC content: 57.15

Gene sequence:

>1461_bases
ATGAGCACTTTTTATACAGTAATCAGCTGGCTGGCGATCTTTGGCTACTGGCTGCTTATCGCCAGCGTCACGTTACGGAT
ATTGATGAAGCGTCGGGCTGTTCCCTCTGCCATGGCGTGGCTGCTGATCATTTACATCCTGCCACTGGTCGGCATTATCG
CCTATCTCTCCTTTGGTGAGCTGCATCTGGGAAAACGCCGCGCCGAGCGCGCGCGTACGATGTGGCCATCCACCGCACGC
TGGTTGGCCGATCTGCGCAGCTGCCGCAGCATTTTTGCCACCCACAACAGCGAGGTCGCCGCACCGCTGTTCCAGCTGTG
TGAACGGCGCCAGGGTATCGCCGGTGTTAAGGGTAACCAGCTACAGCTGCTGACCTCTACCGATGATACGCTCAATGCCC
TGATCCGCGATATTGAGCTGGCGCATACCAGCATTGAGATGATGTTTTATATTTGGCAACCCGGTGGATTGGCCGATCGG
GTTGCCGAAGCGATGATGGCAGCAGCAAGACGCGGCATACGTTGCCGCCTGATGCTCGATTCGGCCGGCAGCGTGGCATT
TTTCCGCAGCCCTTATCCAGCCATGATGCGCGAAGCGGGTATTGAGGTCGTAGAGGCGCTGCAGGTCAACCTGCTGCGTG
TTTTCCTGCGCCGTATGGATCTACGTCAGCACCGCAAAGTGGTGCTGATCGACAACTTTATCGCCTATACCGGCAGCATG
AACCTGGTTGACCCGCGCTACTTTAAGCAGGACGCCGGTGTCGGCCAGTGGATCGATGTCATGGCGCGCATGGAGGGGCC
GGTCGCAACCACCATGGGCATCTACTATGCCTTCGACTGGGAGATGGAGACCGGTAAACGTATTCTGCCACCGGAGCCGA
TATGCAATATTCTCCCCTTTGAAAAAGAGTCGGGCCACACCATTCAGGTGATCGCCTCCGGCCCCGGCTTTCCGGAAGAG
CTGATCCACCAATCACTGTTGACCGCCGTCTACTCCGCGCGCCAACAGCTGGTGATGACCACACCCTATTTTGTTCCCAG
TGACGACCTGTTGCATGCCATCTGTACCGCCGCCCAGCGCGGTGTGGATGTCTCAATCATCGTGCCGAAAAAGAATGACT
CGATGATGGTCGGCTGGGCCAGCCGCGCCTTTTTTACCGAAATGCTGGCCGCCGGCGTTAAAATCTATCAGTTTGAAGGC
GGCCTGTTGCATACCAAGAGCGTGCTGGTCGACGGCCAGCTCAGTCTGGTCGGTACCGTCAACCTGGATATGCGCAGCCT
GTGGCTTAATTTCGAAATTACGCTGGTCATCGACGACGACGGCTTCGGCAGCGATCTCGCCTGCGTACAAGAGGACTATA
TCGCCCGCTCCTCCCTGCTGGACCCACTGGAGTGGTTAAAACGCCCACTGTGGCAACGCGTCGTCGAACGCCTCTTTTAC
TTTTTCAGCCCTTTGCTGTAA

Upstream 100 bases:

>100_bases
ACGCCACGAACTGTATATATTTAGCCGCTAAAGGTTTCTTCTTTGTATATCTGTGCGTAAACTCTCAATTGATGGATTTG
TGCAAGGACAACCTGCGGTT

Downstream 100 bases:

>100_bases
CCGTCTGCGGTCGGCCTACGCCCGACTGCGGCCCCCAATGAGATACTGACATGATGGATTTAAACAACCGCCTGAACGAA
GAAGAGACCCTGGATCAGGC

Product: cardiolipin synthetase

Products: NA

Alternate protein names: CL synthase

Number of amino acids: Translated: 486; Mature: 485

Protein sequence:

>486_residues
MSTFYTVISWLAIFGYWLLIASVTLRILMKRRAVPSAMAWLLIIYILPLVGIIAYLSFGELHLGKRRAERARTMWPSTAR
WLADLRSCRSIFATHNSEVAAPLFQLCERRQGIAGVKGNQLQLLTSTDDTLNALIRDIELAHTSIEMMFYIWQPGGLADR
VAEAMMAAARRGIRCRLMLDSAGSVAFFRSPYPAMMREAGIEVVEALQVNLLRVFLRRMDLRQHRKVVLIDNFIAYTGSM
NLVDPRYFKQDAGVGQWIDVMARMEGPVATTMGIYYAFDWEMETGKRILPPEPICNILPFEKESGHTIQVIASGPGFPEE
LIHQSLLTAVYSARQQLVMTTPYFVPSDDLLHAICTAAQRGVDVSIIVPKKNDSMMVGWASRAFFTEMLAAGVKIYQFEG
GLLHTKSVLVDGQLSLVGTVNLDMRSLWLNFEITLVIDDDGFGSDLACVQEDYIARSSLLDPLEWLKRPLWQRVVERLFY
FFSPLL

Sequences:

>Translated_486_residues
MSTFYTVISWLAIFGYWLLIASVTLRILMKRRAVPSAMAWLLIIYILPLVGIIAYLSFGELHLGKRRAERARTMWPSTAR
WLADLRSCRSIFATHNSEVAAPLFQLCERRQGIAGVKGNQLQLLTSTDDTLNALIRDIELAHTSIEMMFYIWQPGGLADR
VAEAMMAAARRGIRCRLMLDSAGSVAFFRSPYPAMMREAGIEVVEALQVNLLRVFLRRMDLRQHRKVVLIDNFIAYTGSM
NLVDPRYFKQDAGVGQWIDVMARMEGPVATTMGIYYAFDWEMETGKRILPPEPICNILPFEKESGHTIQVIASGPGFPEE
LIHQSLLTAVYSARQQLVMTTPYFVPSDDLLHAICTAAQRGVDVSIIVPKKNDSMMVGWASRAFFTEMLAAGVKIYQFEG
GLLHTKSVLVDGQLSLVGTVNLDMRSLWLNFEITLVIDDDGFGSDLACVQEDYIARSSLLDPLEWLKRPLWQRVVERLFY
FFSPLL
>Mature_485_residues
STFYTVISWLAIFGYWLLIASVTLRILMKRRAVPSAMAWLLIIYILPLVGIIAYLSFGELHLGKRRAERARTMWPSTARW
LADLRSCRSIFATHNSEVAAPLFQLCERRQGIAGVKGNQLQLLTSTDDTLNALIRDIELAHTSIEMMFYIWQPGGLADRV
AEAMMAAARRGIRCRLMLDSAGSVAFFRSPYPAMMREAGIEVVEALQVNLLRVFLRRMDLRQHRKVVLIDNFIAYTGSMN
LVDPRYFKQDAGVGQWIDVMARMEGPVATTMGIYYAFDWEMETGKRILPPEPICNILPFEKESGHTIQVIASGPGFPEEL
IHQSLLTAVYSARQQLVMTTPYFVPSDDLLHAICTAAQRGVDVSIIVPKKNDSMMVGWASRAFFTEMLAAGVKIYQFEGG
LLHTKSVLVDGQLSLVGTVNLDMRSLWLNFEITLVIDDDGFGSDLACVQEDYIARSSLLDPLEWLKRPLWQRVVERLFYF
FSPLL

Specific function: Catalyzes the reversible phosphatidyl group transfer from one phosphatidylglycerol molecule to another to form cardiolipin (CL) (diphosphatidylglycerol) and glycerol

COG id: COG1502

COG function: function code I; Phosphatidylserine/phosphatidylglycerophosphate/cardiolipin synthases and related enzymes

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 PLD phosphodiesterase domains

Homologues:

Organism=Escherichia coli, GI1787502, Length=486, Percent_Identity=82.9218106995885, Blast_Score=856, Evalue=0.0,
Organism=Escherichia coli, GI1787007, Length=345, Percent_Identity=27.2463768115942, Blast_Score=104, Evalue=1e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): CLS_EDWI9 (C5BDA3)

Other databases:

- EMBL:   CP001600
- RefSeq:   YP_002933090.1
- GeneID:   7958907
- GenomeReviews:   CP001600_GR
- KEGG:   eic:NT01EI_1674
- OMA:   AYSAHEY
- ProtClustDB:   PRK01642
- HAMAP:   MF_00190
- InterPro:   IPR022924
- InterPro:   IPR001736
- SMART:   SM00155

Pfam domain/function: NA

EC number: 2.7.8.-

Molecular weight: Translated: 54967; Mature: 54836

Theoretical pI: Translated: 7.53; Mature: 7.53

Prosite motif: PS50035 PLD

Important sites: ACT_SITE 224-224 ACT_SITE 226-226 ACT_SITE 231-231 ACT_SITE 404-404 ACT_SITE 406-406 ACT_SITE 411-411

Signals:

None

Transmembrane regions:

HASH(0x1ce93ff4)-; HASH(0x1a8b2378)-;

Cys/Met content:

1.2 %Cys     (Translated Protein)
4.5 %Met     (Translated Protein)
5.8 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
4.3 %Met     (Mature Protein)
5.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSTFYTVISWLAIFGYWLLIASVTLRILMKRRAVPSAMAWLLIIYILPLVGIIAYLSFGE
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHCH
LHLGKRRAERARTMWPSTARWLADLRSCRSIFATHNSEVAAPLFQLCERRQGIAGVKGNQ
HHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCCCCCE
LQLLTSTDDTLNALIRDIELAHTSIEMMFYIWQPGGLADRVAEAMMAAARRGIRCRLMLD
EEEEECCCHHHHHHHHHHHHHHHEEEEEEEEECCCCHHHHHHHHHHHHHHCCCEEEEEEC
SAGSVAFFRSPYPAMMREAGIEVVEALQVNLLRVFLRRMDLRQHRKVVLIDNFIAYTGSM
CCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECHHHHCCCC
NLVDPRYFKQDAGVGQWIDVMARMEGPVATTMGIYYAFDWEMETGKRILPPEPICNILPF
CCCCCHHHHHCCCCCHHHHHHHHCCCCCEEHEEEEEEEEEECCCCCCCCCCCCCCCCCCC
EKESGHTIQVIASGPGFPEELIHQSLLTAVYSARQQLVMTTPYFVPSDDLLHAICTAAQR
CCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHEEEECCCCCCCHHHHHHHHHHHHC
GVDVSIIVPKKNDSMMVGWASRAFFTEMLAAGVKIYQFEGGLLHTKSVLVDGQLSLVGTV
CCEEEEEEECCCCCEEEEEHHHHHHHHHHHCCCEEEEECCCEEEEEEEEECCCEEEEEEE
NLDMRSLWLNFEITLVIDDDGFGSDLACVQEDYIARSSLLDPLEWLKRPLWQRVVERLFY
CCCCEEEEEEEEEEEEECCCCCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHH
FFSPLL
HHHCCH
>Mature Secondary Structure 
STFYTVISWLAIFGYWLLIASVTLRILMKRRAVPSAMAWLLIIYILPLVGIIAYLSFGE
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHCH
LHLGKRRAERARTMWPSTARWLADLRSCRSIFATHNSEVAAPLFQLCERRQGIAGVKGNQ
HHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCCCCCE
LQLLTSTDDTLNALIRDIELAHTSIEMMFYIWQPGGLADRVAEAMMAAARRGIRCRLMLD
EEEEECCCHHHHHHHHHHHHHHHEEEEEEEEECCCCHHHHHHHHHHHHHHCCCEEEEEEC
SAGSVAFFRSPYPAMMREAGIEVVEALQVNLLRVFLRRMDLRQHRKVVLIDNFIAYTGSM
CCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECHHHHCCCC
NLVDPRYFKQDAGVGQWIDVMARMEGPVATTMGIYYAFDWEMETGKRILPPEPICNILPF
CCCCCHHHHHCCCCCHHHHHHHHCCCCCEEHEEEEEEEEEECCCCCCCCCCCCCCCCCCC
EKESGHTIQVIASGPGFPEELIHQSLLTAVYSARQQLVMTTPYFVPSDDLLHAICTAAQR
CCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHEEEECCCCCCCHHHHHHHHHHHHC
GVDVSIIVPKKNDSMMVGWASRAFFTEMLAAGVKIYQFEGGLLHTKSVLVDGQLSLVGTV
CCEEEEEEECCCCCEEEEEHHHHHHHHHHHCCCEEEEECCCEEEEEEEEECCCEEEEEEE
NLDMRSLWLNFEITLVIDDDGFGSDLACVQEDYIARSSLLDPLEWLKRPLWQRVVERLFY
CCCCEEEEEEEEEEEEECCCCCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHH
FFSPLL
HHHCCH

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Transferring phosphorus-containing groups [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA