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The map label for this gene is pykA [H]

Identifier: 238919509

GI number: 238919509

Start: 1566624

End: 1568066

Strand: Reverse

Name: pykA [H]

Synonym: NT01EI_1605

Alternate gene names: 238919509

Gene position: 1568066-1566624 (Counterclockwise)

Preceding gene: 238919510

Following gene: 238919505

Centisome position: 41.13

GC content: 59.11

Gene sequence:

>1443_bases
ATGTCCAGACGGCTCAGAAGAACCAAAATTGTTACCACGCTTGGCCCGGCTACCGATCGCGACAATAATCTGGAAAAGAT
TATCTCTGCCGGTGCCAACGTGATGCGCCTTAACTTTTCACACGGCACGCCGGAAGATCACCAGCAACGCGCCAACAAAG
TCCGTGAAATTGCCGCCAAACTCGGTCGTCATGTCGCCATTCTCGGGGACCTGCAGGGTCCTAAAATCCGGGTATCCACC
TTTAAGGAAGGTAAAGTTTTCCTCAACGTTGGGGACAAGTTCCTGCTGGATGCCACTCTGAACAAGGGCGAAGGCGATAA
AGAAAAGGTCGGCATCGACTATAAGCGGCTGCCGGAAGATGTGGTGCCGGGCGATATCCTGCTGCTCGACGATGGCCGTG
TACAGCTGAAAGTGCTCTCCGTACAGGAAGATAAGGTATTCACCGAGGTCACTGTGGGCGGGCCGCTGTCCAACAACAAA
GGGATCAACAAGCTGGGCGGTGGGCTCTCTGCCGATGCCCTGACGGAAAAGGACAAGGCCGATATCCTGACTGCCGCCAG
GATTGGCGTCGACTATCTGGCTGTCTCCTTCCCACGCTGCGGAGAGGATCTCAACTATGCCCGCCGTCTGGCGCGCGATG
CCGACAGCGAAGCCAAGATCGTGGCCAAAGTCGAGCGCGCCGAGGCGGTCGCCAGCGACGAAGCGATGGATGATGTGATC
CTGGCCGCCGATGTTGTGATGGTCGCTCGTGGCGATCTGGGGGTTGAAATTGGCGATCCGGAGCTGGTGGGGATCCAGAA
AAAACTGATCCGGCGCGCCCGCAAGCTGAACCGTGTCGTGATCACCGCGACCCAGATGATGGAGTCCATGATCACCAACC
CAATGCCGACCCGCGCGGAGGTCATGGACGTAGCCAACGCCGTACTGGACGGTACCGATGCCGTCATGCTGTCTGCCGAA
ACCGCCGCGGGCCAATACCCGGCCGAAACCGTAGCCGCCATGGCGCGCGTCTGCCTGGGCGCAGAGAAGATCCCCAGCAT
CAATATCTCCAAGCACCGTATTGATGACCAGTTCGACAATACGGAAGAGGCCGTCGCCATGTCCGCCATGTACGCGGCCA
ACCACCTCAAGGGGGTAACAGCCATCATCGCGATGACGGAGTCCGGGCGCACTGCGTTGATGATGTCCCGTATCAGCTCC
GGTCTGCCGATCTTCGCCATGTCCCGTCATGAGCGCACCCTGAACCTGACCGCACTGTACCGCGGTGTCACACCGGTGCA
CTTTGACAGCCACACCGACGGCGTTGCCGCGGCTCAGGCTGCGGCCAATCTGCTACGGGATAAAGGGTTCCTGCTCTCCG
GTGATCTGGTGATCGTCACACAGGGTGATCAGATGGATACCATCGGCAGCACCAACACCTGCCGCACCCTGCGCGTAGAG
TAA

Upstream 100 bases:

>100_bases
CCAGTGGGGCTATCCGGGTAATCGCGGCGGCATTGAGCCGGTGCATTTGCCCACCGTTGTATTGGCAATACCAATGCGTT
ACATGTCAATGGAGTTATGC

Downstream 100 bases:

>100_bases
TCAGGTCTGCCATACGCAGCGGGGGCGCCTGGCGCCCCCGTTTTTCATTATGCCTTCGCCACGGCATACAGCATCAACAC
AATGAACAGCACTGTACCTG

Product: pyruvate kinase

Products: NA

Alternate protein names: PK-2 [H]

Number of amino acids: Translated: 480; Mature: 479

Protein sequence:

>480_residues
MSRRLRRTKIVTTLGPATDRDNNLEKIISAGANVMRLNFSHGTPEDHQQRANKVREIAAKLGRHVAILGDLQGPKIRVST
FKEGKVFLNVGDKFLLDATLNKGEGDKEKVGIDYKRLPEDVVPGDILLLDDGRVQLKVLSVQEDKVFTEVTVGGPLSNNK
GINKLGGGLSADALTEKDKADILTAARIGVDYLAVSFPRCGEDLNYARRLARDADSEAKIVAKVERAEAVASDEAMDDVI
LAADVVMVARGDLGVEIGDPELVGIQKKLIRRARKLNRVVITATQMMESMITNPMPTRAEVMDVANAVLDGTDAVMLSAE
TAAGQYPAETVAAMARVCLGAEKIPSINISKHRIDDQFDNTEEAVAMSAMYAANHLKGVTAIIAMTESGRTALMMSRISS
GLPIFAMSRHERTLNLTALYRGVTPVHFDSHTDGVAAAQAAANLLRDKGFLLSGDLVIVTQGDQMDTIGSTNTCRTLRVE

Sequences:

>Translated_480_residues
MSRRLRRTKIVTTLGPATDRDNNLEKIISAGANVMRLNFSHGTPEDHQQRANKVREIAAKLGRHVAILGDLQGPKIRVST
FKEGKVFLNVGDKFLLDATLNKGEGDKEKVGIDYKRLPEDVVPGDILLLDDGRVQLKVLSVQEDKVFTEVTVGGPLSNNK
GINKLGGGLSADALTEKDKADILTAARIGVDYLAVSFPRCGEDLNYARRLARDADSEAKIVAKVERAEAVASDEAMDDVI
LAADVVMVARGDLGVEIGDPELVGIQKKLIRRARKLNRVVITATQMMESMITNPMPTRAEVMDVANAVLDGTDAVMLSAE
TAAGQYPAETVAAMARVCLGAEKIPSINISKHRIDDQFDNTEEAVAMSAMYAANHLKGVTAIIAMTESGRTALMMSRISS
GLPIFAMSRHERTLNLTALYRGVTPVHFDSHTDGVAAAQAAANLLRDKGFLLSGDLVIVTQGDQMDTIGSTNTCRTLRVE
>Mature_479_residues
SRRLRRTKIVTTLGPATDRDNNLEKIISAGANVMRLNFSHGTPEDHQQRANKVREIAAKLGRHVAILGDLQGPKIRVSTF
KEGKVFLNVGDKFLLDATLNKGEGDKEKVGIDYKRLPEDVVPGDILLLDDGRVQLKVLSVQEDKVFTEVTVGGPLSNNKG
INKLGGGLSADALTEKDKADILTAARIGVDYLAVSFPRCGEDLNYARRLARDADSEAKIVAKVERAEAVASDEAMDDVIL
AADVVMVARGDLGVEIGDPELVGIQKKLIRRARKLNRVVITATQMMESMITNPMPTRAEVMDVANAVLDGTDAVMLSAET
AAGQYPAETVAAMARVCLGAEKIPSINISKHRIDDQFDNTEEAVAMSAMYAANHLKGVTAIIAMTESGRTALMMSRISSG
LPIFAMSRHERTLNLTALYRGVTPVHFDSHTDGVAAAQAAANLLRDKGFLLSGDLVIVTQGDQMDTIGSTNTCRTLRVE

Specific function: Glycolysis; final step. [C]

COG id: COG0469

COG function: function code G; Pyruvate kinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the pyruvate kinase family [H]

Homologues:

Organism=Homo sapiens, GI33286422, Length=504, Percent_Identity=36.7063492063492, Blast_Score=252, Evalue=5e-67,
Organism=Homo sapiens, GI33286420, Length=504, Percent_Identity=36.7063492063492, Blast_Score=252, Evalue=5e-67,
Organism=Homo sapiens, GI33286418, Length=497, Percent_Identity=36.6197183098592, Blast_Score=251, Evalue=1e-66,
Organism=Homo sapiens, GI32967597, Length=493, Percent_Identity=34.6855983772819, Blast_Score=236, Evalue=4e-62,
Organism=Homo sapiens, GI10835121, Length=493, Percent_Identity=34.6855983772819, Blast_Score=236, Evalue=4e-62,
Organism=Homo sapiens, GI310128732, Length=289, Percent_Identity=34.2560553633218, Blast_Score=144, Evalue=2e-34,
Organism=Homo sapiens, GI310128730, Length=289, Percent_Identity=34.2560553633218, Blast_Score=144, Evalue=2e-34,
Organism=Homo sapiens, GI310128736, Length=235, Percent_Identity=33.1914893617021, Blast_Score=112, Evalue=6e-25,
Organism=Homo sapiens, GI310128734, Length=235, Percent_Identity=33.1914893617021, Blast_Score=112, Evalue=6e-25,
Organism=Homo sapiens, GI310128738, Length=214, Percent_Identity=32.7102803738318, Blast_Score=96, Evalue=7e-20,
Organism=Escherichia coli, GI1788160, Length=480, Percent_Identity=89.375, Blast_Score=870, Evalue=0.0,
Organism=Escherichia coli, GI1787965, Length=476, Percent_Identity=37.3949579831933, Blast_Score=280, Evalue=1e-76,
Organism=Caenorhabditis elegans, GI17544584, Length=488, Percent_Identity=36.6803278688525, Blast_Score=271, Evalue=8e-73,
Organism=Caenorhabditis elegans, GI17506831, Length=493, Percent_Identity=36.7139959432049, Blast_Score=246, Evalue=3e-65,
Organism=Caenorhabditis elegans, GI71984413, Length=493, Percent_Identity=36.7139959432049, Blast_Score=245, Evalue=3e-65,
Organism=Caenorhabditis elegans, GI17506829, Length=493, Percent_Identity=36.7139959432049, Blast_Score=245, Evalue=3e-65,
Organism=Caenorhabditis elegans, GI71984406, Length=493, Percent_Identity=36.7139959432049, Blast_Score=245, Evalue=3e-65,
Organism=Saccharomyces cerevisiae, GI6324923, Length=489, Percent_Identity=35.3783231083845, Blast_Score=251, Evalue=1e-67,
Organism=Saccharomyces cerevisiae, GI6319279, Length=475, Percent_Identity=35.3684210526316, Blast_Score=247, Evalue=2e-66,
Organism=Drosophila melanogaster, GI24648964, Length=496, Percent_Identity=36.6935483870968, Blast_Score=264, Evalue=1e-70,
Organism=Drosophila melanogaster, GI28571814, Length=496, Percent_Identity=36.6935483870968, Blast_Score=264, Evalue=1e-70,
Organism=Drosophila melanogaster, GI24581235, Length=493, Percent_Identity=32.4543610547667, Blast_Score=228, Evalue=5e-60,
Organism=Drosophila melanogaster, GI24648966, Length=417, Percent_Identity=35.0119904076739, Blast_Score=228, Evalue=7e-60,
Organism=Drosophila melanogaster, GI24646914, Length=277, Percent_Identity=36.101083032491, Blast_Score=142, Evalue=4e-34,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001697
- InterPro:   IPR015813
- InterPro:   IPR011037
- InterPro:   IPR015794
- InterPro:   IPR018209
- InterPro:   IPR015793
- InterPro:   IPR015795
- InterPro:   IPR015806 [H]

Pfam domain/function: PF00224 PK; PF02887 PK_C [H]

EC number: =2.7.1.40 [H]

Molecular weight: Translated: 51799; Mature: 51668

Theoretical pI: Translated: 6.53; Mature: 6.53

Prosite motif: PS00110 PYRUVATE_KINASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSRRLRRTKIVTTLGPATDRDNNLEKIISAGANVMRLNFSHGTPEDHQQRANKVREIAAK
CCCCCCHHEEEEEECCCCCCCCCHHHHHHCCCCEEEEEECCCCCHHHHHHHHHHHHHHHH
LGRHVAILGDLQGPKIRVSTFKEGKVFLNVGDKFLLDATLNKGEGDKEKVGIDYKRLPED
HCCEEEEEECCCCCEEEEEEECCCEEEEEECCEEEEEEECCCCCCCHHHCCCCHHHCCHH
VVPGDILLLDDGRVQLKVLSVQEDKVFTEVTVGGPLSNNKGINKLGGGLSADALTEKDKA
CCCCCEEEEECCCEEEEEEEEECCCEEEEEEECCCCCCCCCCHHHCCCCCCHHHCCCCHH
DILTAARIGVDYLAVSFPRCGEDLNYARRLARDADSEAKIVAKVERAEAVASDEAMDDVI
HHHHHHHHCHHHHEEECCCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHH
LAADVVMVARGDLGVEIGDPELVGIQKKLIRRARKLNRVVITATQMMESMITNPMPTRAE
HHHHEEEEECCCCCEEECCCCEEHHHHHHHHHHHHHCEEEEEHHHHHHHHHCCCCCCHHH
VMDVANAVLDGTDAVMLSAETAAGQYPAETVAAMARVCLGAEKIPSINISKHRIDDQFDN
HHHHHHHHHCCCCEEEEEECCCCCCCCHHHHHHHHHHHHCHHHCCCCCCHHHHCCCCCCC
TEEAVAMSAMYAANHLKGVTAIIAMTESGRTALMMSRISSGLPIFAMSRHERTLNLTALY
HHHHHHHHHHHHHHHHCCEEEEEEEECCCCHHHHHHHHCCCCCEEEECCCCCEEEEEEHC
RGVTPVHFDSHTDGVAAAQAAANLLRDKGFLLSGDLVIVTQGDQMDTIGSTNTCRTLRVE
CCCCCEEECCCCCHHHHHHHHHHHHHCCCCEEECCEEEEECCCCCCCCCCCCCCEEEEEC
>Mature Secondary Structure 
SRRLRRTKIVTTLGPATDRDNNLEKIISAGANVMRLNFSHGTPEDHQQRANKVREIAAK
CCCCCHHEEEEEECCCCCCCCCHHHHHHCCCCEEEEEECCCCCHHHHHHHHHHHHHHHH
LGRHVAILGDLQGPKIRVSTFKEGKVFLNVGDKFLLDATLNKGEGDKEKVGIDYKRLPED
HCCEEEEEECCCCCEEEEEEECCCEEEEEECCEEEEEEECCCCCCCHHHCCCCHHHCCHH
VVPGDILLLDDGRVQLKVLSVQEDKVFTEVTVGGPLSNNKGINKLGGGLSADALTEKDKA
CCCCCEEEEECCCEEEEEEEEECCCEEEEEEECCCCCCCCCCHHHCCCCCCHHHCCCCHH
DILTAARIGVDYLAVSFPRCGEDLNYARRLARDADSEAKIVAKVERAEAVASDEAMDDVI
HHHHHHHHCHHHHEEECCCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHH
LAADVVMVARGDLGVEIGDPELVGIQKKLIRRARKLNRVVITATQMMESMITNPMPTRAE
HHHHEEEEECCCCCEEECCCCEEHHHHHHHHHHHHHCEEEEEHHHHHHHHHCCCCCCHHH
VMDVANAVLDGTDAVMLSAETAAGQYPAETVAAMARVCLGAEKIPSINISKHRIDDQFDN
HHHHHHHHHCCCCEEEEEECCCCCCCCHHHHHHHHHHHHCHHHCCCCCCHHHHCCCCCCC
TEEAVAMSAMYAANHLKGVTAIIAMTESGRTALMMSRISSGLPIFAMSRHERTLNLTALY
HHHHHHHHHHHHHHHHCCEEEEEEEECCCCHHHHHHHHCCCCCEEEECCCCCEEEEEEHC
RGVTPVHFDSHTDGVAAAQAAANLLRDKGFLLSGDLVIVTQGDQMDTIGSTNTCRTLRVE
CCCCCEEECCCCCHHHHHHHHHHHHHCCCCEEECCEEEEECCCCCCCCCCCCCCEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9097040; 9278503; 1732206; 1859631; 8439398 [H]