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The map label for this gene is engD [H]

Identifier: 238919461

GI number: 238919461

Start: 1517598

End: 1518689

Strand: Reverse

Name: engD [H]

Synonym: NT01EI_1555

Alternate gene names: 238919461

Gene position: 1518689-1517598 (Counterclockwise)

Preceding gene: 238919462

Following gene: 238919460

Centisome position: 39.84

GC content: 57.23

Gene sequence:

>1092_bases
ATGGGTTTTAAATGCGGTATCGTCGGGCTTCCCAACGTCGGTAAATCCACGCTGTTCAATGCGCTGACCAAAGCGGGGAT
CGAAGCAGCCAACTTCCCGTTCTGTACCATTGAGCCGAACACCGGTGTGGTACCGATGCCGGATCCGCGTCTGGATCAGC
TGGCCGAGATCGTGAAACCCCAGCGTACCCTGCCGACCACCATGGAGTTTGTCGACATTGCCGGCCTGGTCAAAGGCGCT
TCCAAAGGTGAAGGGCTGGGCAACCAGTTCCTGACCAACATCCGTGAAACCGAAGCTATCGGCCACGTGGTGCGCTGCTT
TGAAAACGACAATATCGTACACGTTTCCGGCCGCGTAAACCCGGCGGAAGACATCGAGGTGATCAACACCGAGCTGGCAC
TGGCGGATCTGGATACCTGTGAGCGTGCAATTCACCGCGTGCAGAAGAAAGCCAAAGGCGGCGATAAAGACGCCAAGCTG
GAGCTCTCCGCGCTGGAAAAATGCCTGCCGCACCTGGAGCAGGCCGGTATGCTGCGTGCACTGGACCTCAGCGATGAAGA
GAAAGGGGCTATCAAATACCTGAGCTTCCTGACCCTAAAACCGACCATGTATATCGCCAACGTCAACGAAGATGGCTTTG
AGAACAATCCGTTCCTGGATCAGGTGCGCGCCATCGCCGACGCAGAGGGCTCCGTTGTGGTTCCGGTATGTGCCGCCGTT
GAGGCCGACATCGCCGAGCTGGACGACGCCGATCGCGACGAGTTCATGGCCGAACTGGGTCTGGAAGAGCCGGGGCTGAA
CCGCGTGATCCGCGCCGGCTACACGCTGCTGAACCTGCAGACCTACTTCACCGCCGGGGTAAAAGAGGTTCGTGCCTGGA
CCATTCCCGTCGGCGCCACCGCCCCGCAGGCCGCCGGTAAAATCCATACCGACTTTGAAAAAGGTTTTATCCGCGCCCAG
ACCATCGCCTTTGACGATTTCATCACCTATAAGGGTGAACAGGGTGCCAAAGAAGCCGGTAAGATGCGTTCTGAGGGTAA
GGACTATATCGTCAAGGATGGCGATATCATGAACTTCCTGTTTAACGTCTAA

Upstream 100 bases:

>100_bases
AGAAAATGACGCGTTGCGTGTATAATCGGGCGATATTTTCCTGTTTCTTTGGCGGTTATTCTATAGATCGCTAAAAATCA
ATGAGTTAAGGTGATTAAAC

Downstream 100 bases:

>100_bases
ATAAACTTTGCCGTCTCATGAGATTTCACTGAATCTCATGACGATTAACAAACCCAATAAAATCCACGCACTTGCGTGGA
TTTTTTATTTCATGGTATCT

Product: GTP-dependent nucleic acid-binding protein EngD

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 363; Mature: 362

Protein sequence:

>363_residues
MGFKCGIVGLPNVGKSTLFNALTKAGIEAANFPFCTIEPNTGVVPMPDPRLDQLAEIVKPQRTLPTTMEFVDIAGLVKGA
SKGEGLGNQFLTNIRETEAIGHVVRCFENDNIVHVSGRVNPAEDIEVINTELALADLDTCERAIHRVQKKAKGGDKDAKL
ELSALEKCLPHLEQAGMLRALDLSDEEKGAIKYLSFLTLKPTMYIANVNEDGFENNPFLDQVRAIADAEGSVVVPVCAAV
EADIAELDDADRDEFMAELGLEEPGLNRVIRAGYTLLNLQTYFTAGVKEVRAWTIPVGATAPQAAGKIHTDFEKGFIRAQ
TIAFDDFITYKGEQGAKEAGKMRSEGKDYIVKDGDIMNFLFNV

Sequences:

>Translated_363_residues
MGFKCGIVGLPNVGKSTLFNALTKAGIEAANFPFCTIEPNTGVVPMPDPRLDQLAEIVKPQRTLPTTMEFVDIAGLVKGA
SKGEGLGNQFLTNIRETEAIGHVVRCFENDNIVHVSGRVNPAEDIEVINTELALADLDTCERAIHRVQKKAKGGDKDAKL
ELSALEKCLPHLEQAGMLRALDLSDEEKGAIKYLSFLTLKPTMYIANVNEDGFENNPFLDQVRAIADAEGSVVVPVCAAV
EADIAELDDADRDEFMAELGLEEPGLNRVIRAGYTLLNLQTYFTAGVKEVRAWTIPVGATAPQAAGKIHTDFEKGFIRAQ
TIAFDDFITYKGEQGAKEAGKMRSEGKDYIVKDGDIMNFLFNV
>Mature_362_residues
GFKCGIVGLPNVGKSTLFNALTKAGIEAANFPFCTIEPNTGVVPMPDPRLDQLAEIVKPQRTLPTTMEFVDIAGLVKGAS
KGEGLGNQFLTNIRETEAIGHVVRCFENDNIVHVSGRVNPAEDIEVINTELALADLDTCERAIHRVQKKAKGGDKDAKLE
LSALEKCLPHLEQAGMLRALDLSDEEKGAIKYLSFLTLKPTMYIANVNEDGFENNPFLDQVRAIADAEGSVVVPVCAAVE
ADIAELDDADRDEFMAELGLEEPGLNRVIRAGYTLLNLQTYFTAGVKEVRAWTIPVGATAPQAAGKIHTDFEKGFIRAQT
IAFDDFITYKGEQGAKEAGKMRSEGKDYIVKDGDIMNFLFNV

Specific function: GTP-dependent nucleic acid-binding protein which may act as a translation factor [H]

COG id: COG0012

COG function: function code J; Predicted GTPase, probable translation factor

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 G (guanine nucleotide-binding) domain [H]

Homologues:

Organism=Homo sapiens, GI58761500, Length=368, Percent_Identity=44.8369565217391, Blast_Score=306, Evalue=2e-83,
Organism=Homo sapiens, GI58761502, Length=226, Percent_Identity=39.8230088495575, Blast_Score=155, Evalue=4e-38,
Organism=Escherichia coli, GI1787454, Length=363, Percent_Identity=93.66391184573, Blast_Score=703, Evalue=0.0,
Organism=Escherichia coli, GI1789574, Length=127, Percent_Identity=32.2834645669291, Blast_Score=66, Evalue=4e-12,
Organism=Caenorhabditis elegans, GI17509631, Length=365, Percent_Identity=41.0958904109589, Blast_Score=291, Evalue=3e-79,
Organism=Caenorhabditis elegans, GI17552324, Length=173, Percent_Identity=28.3236994219653, Blast_Score=65, Evalue=6e-11,
Organism=Saccharomyces cerevisiae, GI6319499, Length=368, Percent_Identity=39.1304347826087, Blast_Score=254, Evalue=1e-68,
Organism=Saccharomyces cerevisiae, GI6321773, Length=379, Percent_Identity=34.3007915567282, Blast_Score=194, Evalue=1e-50,
Organism=Saccharomyces cerevisiae, GI6321649, Length=142, Percent_Identity=38.0281690140845, Blast_Score=79, Evalue=8e-16,
Organism=Drosophila melanogaster, GI24640873, Length=366, Percent_Identity=43.4426229508197, Blast_Score=300, Evalue=8e-82,
Organism=Drosophila melanogaster, GI24640877, Length=366, Percent_Identity=43.4426229508197, Blast_Score=300, Evalue=8e-82,
Organism=Drosophila melanogaster, GI24640875, Length=366, Percent_Identity=43.4426229508197, Blast_Score=300, Evalue=8e-82,
Organism=Drosophila melanogaster, GI24640879, Length=328, Percent_Identity=41.1585365853659, Blast_Score=246, Evalue=2e-65,

Paralogues:

None

Copy number: 120 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 140 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012675
- InterPro:   IPR004396
- InterPro:   IPR013029
- InterPro:   IPR006073
- InterPro:   IPR002917
- InterPro:   IPR012676
- InterPro:   IPR023192 [H]

Pfam domain/function: PF01926 MMR_HSR1; PF06071 YchF-GTPase_C [H]

EC number: NA

Molecular weight: Translated: 39507; Mature: 39376

Theoretical pI: Translated: 4.54; Mature: 4.54

Prosite motif: PS00443 GATASE_TYPE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGFKCGIVGLPNVGKSTLFNALTKAGIEAANFPFCTIEPNTGVVPMPDPRLDQLAEIVKP
CCEEECEEECCCCCHHHHHHHHHHHCCCCCCCCEEEECCCCCCCCCCCCCHHHHHHHHCC
QRTLPTTMEFVDIAGLVKGASKGEGLGNQFLTNIRETEAIGHVVRCFENDNIVHVSGRVN
CCCCCHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCC
PAEDIEVINTELALADLDTCERAIHRVQKKAKGGDKDAKLELSALEKCLPHLEQAGMLRA
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEHHHHHHHHHHHHHCCCEEE
LDLSDEEKGAIKYLSFLTLKPTMYIANVNEDGFENNPFLDQVRAIADAEGSVVVPVCAAV
ECCCCCCCCHHHHHHHEEECCEEEEEECCCCCCCCCCHHHHHHHHHCCCCCEEEEEHHHH
EADIAELDDADRDEFMAELGLEEPGLNRVIRAGYTLLNLQTYFTAGVKEVRAWTIPVGAT
HHHHHHCCCCCHHHHHHHHCCCCCCHHHHHHHCCCEEEHHHHHHHHHHHHEEEEEECCCC
APQAAGKIHTDFEKGFIRAQTIAFDDFITYKGEQGAKEAGKMRSEGKDYIVKDGDIMNFL
CCHHCCCCCCHHHHCCEEEEEEEEEHHEEECCCCCHHHHHHHHHCCCCEEEECCHHHHHH
FNV
HCC
>Mature Secondary Structure 
GFKCGIVGLPNVGKSTLFNALTKAGIEAANFPFCTIEPNTGVVPMPDPRLDQLAEIVKP
CEEECEEECCCCCHHHHHHHHHHHCCCCCCCCEEEECCCCCCCCCCCCCHHHHHHHHCC
QRTLPTTMEFVDIAGLVKGASKGEGLGNQFLTNIRETEAIGHVVRCFENDNIVHVSGRVN
CCCCCHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCC
PAEDIEVINTELALADLDTCERAIHRVQKKAKGGDKDAKLELSALEKCLPHLEQAGMLRA
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEHHHHHHHHHHHHHCCCEEE
LDLSDEEKGAIKYLSFLTLKPTMYIANVNEDGFENNPFLDQVRAIADAEGSVVVPVCAAV
ECCCCCCCCHHHHHHHEEECCEEEEEECCCCCCCCCCHHHHHHHHHCCCCCEEEEEHHHH
EADIAELDDADRDEFMAELGLEEPGLNRVIRAGYTLLNLQTYFTAGVKEVRAWTIPVGAT
HHHHHHCCCCCHHHHHHHHCCCCCCHHHHHHHCCCEEEHHHHHHHHHHHHEEEEEECCCC
APQAAGKIHTDFEKGFIRAQTIAFDDFITYKGEQGAKEAGKMRSEGKDYIVKDGDIMNFL
CCHHCCCCCCHHHHCCEEEEEEEEEHHEEECCCCCHHHHHHHHHCCCCEEEECCHHHHHH
FNV
HCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]