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The map label for this gene is ansP [H]

Identifier: 238919350

GI number: 238919350

Start: 1420509

End: 1421975

Strand: Direct

Name: ansP [H]

Synonym: NT01EI_1444

Alternate gene names: 238919350

Gene position: 1420509-1421975 (Clockwise)

Preceding gene: 238919349

Following gene: 238919351

Centisome position: 37.26

GC content: 63.6

Gene sequence:

>1467_bases
ATGAATTTACAGGGTAAGGGCGCAGAGGCGCAGGCAGACCGGCAGCGGCGTGCGCTGGATGCCCATGAGGCGGGTTACCA
GAAGGGGATGGGAAAGCGGCAGATCCAGATGATCGCCATCGGTGGTGCCATCGGCACCGGACTGTTTCTGGGCACCGGTG
GGCGCCTACAGGCGGCGGGGCCTTCACTGGCGCTGGTGTATCTGATCTGCGGGCTGTTTTCGTTTTTTATTCTGCGCGCA
CTGGGTGAGCTGGTACTGCATCGCCCGGCCAGCGGCAGCTTTGTCTCCTATGCGCGGGAGTTTTTGGGGGAGAAGGCGGC
CTACGTGGCCGGATGGATGTATTTTCTCAACTGGGCGATGACCGGCATCGTCGATATCACCGCGGTAGCGCTGTATATGC
ACTATTGGGGCGCCTTTGCCGGGGTGCCTCAGTGGCTTTTTGCCCTGATGGCGCTGACGCTGGTCGCGACCATGAATATG
ATCGGCGTGAAGTGGTTTGCCGAGATGGAGTTCTGGTTTGCCCTGATTAAGGTGGTGGCTATCGTTCTGTTTCTGCTGGT
CGGTACCCTGTATCTGGGCAGCGGTCGCCCGCTGGCGGGGAGTCCGACCGGGCTGCATCTGATCACCGATAACGGTGGTC
TGTTTCCACACGGCGTGCTGCCGGCGCTGGTCTTGATCCAGGGGGTGGTCTTTGCCTTCGCCTCGATCGAGCTGGTCGGT
ACCGCCGCCGGTGAGTGCCGCGATCCGCAGCGCCTGGTACCCCGGGCGATCAACAGCGTTATCTGGCGTATCGGCCTGTT
TTACGTCGGCTCGGTCCTGCTGCTGGTCTGCCTGCTGCCGTGGAATGTCTATCAGGCCGGTCAGAGCCCGTTTGTCACCT
TCTTCTCCCGGCTGGGCGTGCCTTACGTCGGCAGCCTGATGAACCTGGTCGTGCTGACGGCGGCGCTTTCCAGCCTGAAC
TCCGGGCTGTACTGCACCGGTCGGATCCTGCGCTCGCTGGCGATGGGCGGTTCGGCGCCGCGCTTTATGGCCCGGATGAG
CGCCCAGGCGGTGCCGTACGCCGGGATCCTGGCCACCGTCGCCCTGTATATGGTGGGCGTGGTGCTTAATTATCTGCTGC
CGAGCCGGGTATTCGAGATCGTGCTCAACGTAGCCTCGCTGGGGATCATCGCCTCCTGGGCATTTATCATCGTCTGTCAG
ATGAAGCTGCGCCAGGCGATCCGCGCCGGGCGAGCCAAACCGGTGGCCTTCCGGATGCCCGGCGCCCCCGTTACCGGTTG
GCTGACGCTGGCATTTCTGCTGGGGGTCGTGGTGCTGATGGCCTGTGACTATCCGAACGGGACCTGGACGGTCGCCACGC
TGCCGCTGTTGGTGCTGTTGCTGGCACTGGGCTGGCGCGGGCTGCACCGCTGCGAACAGCAGGGTGACGTGTCGGCTCTG
CCCGCCGACTGCGAGGCCGGGCAATGA

Upstream 100 bases:

>100_bases
GGATACACGGGCTCAGAGAGGCCTGGGGCACGGCGTGTCGCGCAGCGGGATAATCCCGGGGCGGGGCATGACAAACAGAC
AGAAAATAGGTTATTGAAGA

Downstream 100 bases:

>100_bases
ATACTGGCTGCGTGCCCGGCTCATATTTGCTAACACAATGGCGGGCGGGTGAGACGCATCCCACCACGGCCTCAGTTACA
CTGAGCTACCGGTGGCGATG

Product: L-asparagine permease

Products: Proton [Cytoplasm]; L-asparagine [Cytoplasm] [C]

Alternate protein names: L-asparagine transport protein [H]

Number of amino acids: Translated: 488; Mature: 488

Protein sequence:

>488_residues
MNLQGKGAEAQADRQRRALDAHEAGYQKGMGKRQIQMIAIGGAIGTGLFLGTGGRLQAAGPSLALVYLICGLFSFFILRA
LGELVLHRPASGSFVSYAREFLGEKAAYVAGWMYFLNWAMTGIVDITAVALYMHYWGAFAGVPQWLFALMALTLVATMNM
IGVKWFAEMEFWFALIKVVAIVLFLLVGTLYLGSGRPLAGSPTGLHLITDNGGLFPHGVLPALVLIQGVVFAFASIELVG
TAAGECRDPQRLVPRAINSVIWRIGLFYVGSVLLLVCLLPWNVYQAGQSPFVTFFSRLGVPYVGSLMNLVVLTAALSSLN
SGLYCTGRILRSLAMGGSAPRFMARMSAQAVPYAGILATVALYMVGVVLNYLLPSRVFEIVLNVASLGIIASWAFIIVCQ
MKLRQAIRAGRAKPVAFRMPGAPVTGWLTLAFLLGVVVLMACDYPNGTWTVATLPLLVLLLALGWRGLHRCEQQGDVSAL
PADCEAGQ

Sequences:

>Translated_488_residues
MNLQGKGAEAQADRQRRALDAHEAGYQKGMGKRQIQMIAIGGAIGTGLFLGTGGRLQAAGPSLALVYLICGLFSFFILRA
LGELVLHRPASGSFVSYAREFLGEKAAYVAGWMYFLNWAMTGIVDITAVALYMHYWGAFAGVPQWLFALMALTLVATMNM
IGVKWFAEMEFWFALIKVVAIVLFLLVGTLYLGSGRPLAGSPTGLHLITDNGGLFPHGVLPALVLIQGVVFAFASIELVG
TAAGECRDPQRLVPRAINSVIWRIGLFYVGSVLLLVCLLPWNVYQAGQSPFVTFFSRLGVPYVGSLMNLVVLTAALSSLN
SGLYCTGRILRSLAMGGSAPRFMARMSAQAVPYAGILATVALYMVGVVLNYLLPSRVFEIVLNVASLGIIASWAFIIVCQ
MKLRQAIRAGRAKPVAFRMPGAPVTGWLTLAFLLGVVVLMACDYPNGTWTVATLPLLVLLLALGWRGLHRCEQQGDVSAL
PADCEAGQ
>Mature_488_residues
MNLQGKGAEAQADRQRRALDAHEAGYQKGMGKRQIQMIAIGGAIGTGLFLGTGGRLQAAGPSLALVYLICGLFSFFILRA
LGELVLHRPASGSFVSYAREFLGEKAAYVAGWMYFLNWAMTGIVDITAVALYMHYWGAFAGVPQWLFALMALTLVATMNM
IGVKWFAEMEFWFALIKVVAIVLFLLVGTLYLGSGRPLAGSPTGLHLITDNGGLFPHGVLPALVLIQGVVFAFASIELVG
TAAGECRDPQRLVPRAINSVIWRIGLFYVGSVLLLVCLLPWNVYQAGQSPFVTFFSRLGVPYVGSLMNLVVLTAALSSLN
SGLYCTGRILRSLAMGGSAPRFMARMSAQAVPYAGILATVALYMVGVVLNYLLPSRVFEIVLNVASLGIIASWAFIIVCQ
MKLRQAIRAGRAKPVAFRMPGAPVTGWLTLAFLLGVVVLMACDYPNGTWTVATLPLLVLLLALGWRGLHRCEQQGDVSAL
PADCEAGQ

Specific function: Unknown

COG id: COG1113

COG function: function code E; Gamma-aminobutyrate permease and related permeases

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the amino acid-polyamine-organocation (APC) superfamily. Amino acid transporter (AAT) (TC 2.A.3.1) family [H]

Homologues:

Organism=Homo sapiens, GI258645169, Length=429, Percent_Identity=22.8438228438228, Blast_Score=69, Evalue=9e-12,
Organism=Homo sapiens, GI4507047, Length=428, Percent_Identity=21.2616822429907, Blast_Score=67, Evalue=4e-11,
Organism=Escherichia coli, GI87081915, Length=490, Percent_Identity=76.734693877551, Blast_Score=753, Evalue=0.0,
Organism=Escherichia coli, GI1786302, Length=450, Percent_Identity=36.8888888888889, Blast_Score=307, Evalue=1e-84,
Organism=Escherichia coli, GI1790653, Length=461, Percent_Identity=39.2624728850325, Blast_Score=305, Evalue=3e-84,
Organism=Escherichia coli, GI1786602, Length=448, Percent_Identity=36.3839285714286, Blast_Score=303, Evalue=2e-83,
Organism=Escherichia coli, GI1786789, Length=449, Percent_Identity=35.6347438752784, Blast_Score=302, Evalue=3e-83,
Organism=Escherichia coli, GI48994972, Length=421, Percent_Identity=38.479809976247, Blast_Score=287, Evalue=1e-78,
Organism=Escherichia coli, GI1789017, Length=420, Percent_Identity=33.3333333333333, Blast_Score=245, Evalue=5e-66,
Organism=Escherichia coli, GI87081708, Length=431, Percent_Identity=35.7308584686775, Blast_Score=242, Evalue=3e-65,
Organism=Escherichia coli, GI1788480, Length=434, Percent_Identity=33.1797235023041, Blast_Score=235, Evalue=6e-63,
Organism=Escherichia coli, GI87081869, Length=330, Percent_Identity=23.030303030303, Blast_Score=62, Evalue=8e-11,
Organism=Caenorhabditis elegans, GI17532491, Length=405, Percent_Identity=22.4691358024691, Blast_Score=70, Evalue=4e-12,
Organism=Saccharomyces cerevisiae, GI6324061, Length=515, Percent_Identity=26.9902912621359, Blast_Score=199, Evalue=7e-52,
Organism=Saccharomyces cerevisiae, GI6320772, Length=425, Percent_Identity=31.5294117647059, Blast_Score=199, Evalue=1e-51,
Organism=Saccharomyces cerevisiae, GI6322892, Length=435, Percent_Identity=31.0344827586207, Blast_Score=182, Evalue=1e-46,
Organism=Saccharomyces cerevisiae, GI6324990, Length=430, Percent_Identity=28.1395348837209, Blast_Score=167, Evalue=2e-42,
Organism=Saccharomyces cerevisiae, GI6322967, Length=415, Percent_Identity=28.433734939759, Blast_Score=160, Evalue=3e-40,
Organism=Saccharomyces cerevisiae, GI6321629, Length=427, Percent_Identity=27.6346604215457, Blast_Score=160, Evalue=4e-40,
Organism=Saccharomyces cerevisiae, GI6324924, Length=446, Percent_Identity=27.5784753363229, Blast_Score=159, Evalue=1e-39,
Organism=Saccharomyces cerevisiae, GI6324059, Length=422, Percent_Identity=29.1469194312796, Blast_Score=157, Evalue=2e-39,
Organism=Saccharomyces cerevisiae, GI6321053, Length=420, Percent_Identity=25.7142857142857, Blast_Score=146, Evalue=7e-36,
Organism=Saccharomyces cerevisiae, GI6324553, Length=409, Percent_Identity=28.8508557457213, Blast_Score=146, Evalue=7e-36,
Organism=Saccharomyces cerevisiae, GI6324981, Length=423, Percent_Identity=28.8416075650118, Blast_Score=145, Evalue=2e-35,
Organism=Saccharomyces cerevisiae, GI6320717, Length=411, Percent_Identity=27.007299270073, Blast_Score=135, Evalue=1e-32,
Organism=Saccharomyces cerevisiae, GI6319824, Length=436, Percent_Identity=25.6880733944954, Blast_Score=133, Evalue=6e-32,
Organism=Saccharomyces cerevisiae, GI6319608, Length=457, Percent_Identity=26.4770240700219, Blast_Score=127, Evalue=4e-30,
Organism=Saccharomyces cerevisiae, GI6319543, Length=415, Percent_Identity=26.0240963855422, Blast_Score=127, Evalue=5e-30,
Organism=Saccharomyces cerevisiae, GI6320251, Length=403, Percent_Identity=24.0694789081886, Blast_Score=122, Evalue=1e-28,
Organism=Saccharomyces cerevisiae, GI6319542, Length=408, Percent_Identity=23.7745098039216, Blast_Score=119, Evalue=2e-27,
Organism=Drosophila melanogaster, GI221512776, Length=425, Percent_Identity=21.1764705882353, Blast_Score=81, Evalue=1e-15,
Organism=Drosophila melanogaster, GI24666159, Length=425, Percent_Identity=21.1764705882353, Blast_Score=81, Evalue=1e-15,
Organism=Drosophila melanogaster, GI24668806, Length=417, Percent_Identity=21.8225419664269, Blast_Score=70, Evalue=5e-12,
Organism=Drosophila melanogaster, GI21356285, Length=417, Percent_Identity=21.8225419664269, Blast_Score=70, Evalue=5e-12,
Organism=Drosophila melanogaster, GI24668802, Length=417, Percent_Identity=21.8225419664269, Blast_Score=70, Evalue=5e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004841
- InterPro:   IPR002293
- InterPro:   IPR004840 [H]

Pfam domain/function: PF00324 AA_permease [H]

EC number: NA

Molecular weight: Translated: 52431; Mature: 52431

Theoretical pI: Translated: 9.64; Mature: 9.64

Prosite motif: PS00218 AMINO_ACID_PERMEASE_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
5.3 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
5.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNLQGKGAEAQADRQRRALDAHEAGYQKGMGKRQIQMIAIGGAIGTGLFLGTGGRLQAAG
CCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCHHHHHHEECCCCEEECCC
PSLALVYLICGLFSFFILRALGELVLHRPASGSFVSYAREFLGEKAAYVAGWMYFLNWAM
CHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHH
TGIVDITAVALYMHYWGAFAGVPQWLFALMALTLVATMNMIGVKWFAEMEFWFALIKVVA
HHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IVLFLLVGTLYLGSGRPLAGSPTGLHLITDNGGLFPHGVLPALVLIQGVVFAFASIELVG
HHHHHHHHHHHCCCCCCCCCCCCCEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHC
TAAGECRDPQRLVPRAINSVIWRIGLFYVGSVLLLVCLLPWNVYQAGQSPFVTFFSRLGV
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHCCCCHHHHHHHHCCC
PYVGSLMNLVVLTAALSSLNSGLYCTGRILRSLAMGGSAPRFMARMSAQAVPYAGILATV
HHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCHHHHHHHHHHCCCHHHHHHHH
ALYMVGVVLNYLLPSRVFEIVLNVASLGIIASWAFIIVCQMKLRQAIRAGRAKPVAFRMP
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEECC
GAPVTGWLTLAFLLGVVVLMACDYPNGTWTVATLPLLVLLLALGWRGLHRCEQQGDVSAL
CCCHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHCCHHHHHHHHCCCCCCC
PADCEAGQ
CCCCCCCC
>Mature Secondary Structure
MNLQGKGAEAQADRQRRALDAHEAGYQKGMGKRQIQMIAIGGAIGTGLFLGTGGRLQAAG
CCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCHHHHHHEECCCCEEECCC
PSLALVYLICGLFSFFILRALGELVLHRPASGSFVSYAREFLGEKAAYVAGWMYFLNWAM
CHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHH
TGIVDITAVALYMHYWGAFAGVPQWLFALMALTLVATMNMIGVKWFAEMEFWFALIKVVA
HHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IVLFLLVGTLYLGSGRPLAGSPTGLHLITDNGGLFPHGVLPALVLIQGVVFAFASIELVG
HHHHHHHHHHHCCCCCCCCCCCCCEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHC
TAAGECRDPQRLVPRAINSVIWRIGLFYVGSVLLLVCLLPWNVYQAGQSPFVTFFSRLGV
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHCCCCHHHHHHHHCCC
PYVGSLMNLVVLTAALSSLNSGLYCTGRILRSLAMGGSAPRFMARMSAQAVPYAGILATV
HHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCHHHHHHHHHHCCCHHHHHHHH
ALYMVGVVLNYLLPSRVFEIVLNVASLGIIASWAFIIVCQMKLRQAIRAGRAKPVAFRMP
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEECC
GAPVTGWLTLAFLLGVVVLMACDYPNGTWTVATLPLLVLLLALGWRGLHRCEQQGDVSAL
CCCHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHCCHHHHHHHHCCCCCCC
PADCEAGQ
CCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: L-asparagine [Periplasm]; Proton [Periplasm] [C]

Specific reaction: L-asparagine [Periplasm] + Proton [Periplasm] = Proton [Cytoplasm] + L-asparagine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9097039; 9278503 [H]