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The map label for this gene is yeiE [H]

Identifier: 238919210

GI number: 238919210

Start: 1280099

End: 1280965

Strand: Direct

Name: yeiE [H]

Synonym: NT01EI_1293

Alternate gene names: 238919210

Gene position: 1280099-1280965 (Clockwise)

Preceding gene: 238919206

Following gene: 238919211

Centisome position: 33.58

GC content: 60.21

Gene sequence:

>867_bases
ATGCACATCACCTTGCGACAACTGGAAGTTTTTACAGAAGTACTGAAAAGCGGTTCGACCACGCAGGCATCCATAATGCT
GGCGCTTTCGCAGTCGGCGGTGAGCGCAGCGCTGTCGGATCTGGAGACCCAGCTGTCGGTGCGTCTGTTCGATCGCGTAG
GTAAACGGCTGGTGGTCAACGAGCACGGTCGTCTGCTGTATCCGAAAGCGATGGCGCTGCTGGAGCAGGCCAGCGAAATC
GAACAGCTGTTTAAGGGTGAGAGCGGAGCGCTGCGCATTGCCGCCAGCAGCACCATCGGTAACTATCTTCTGCCGCCGAT
GATGGCCGCCTACCGGCGCGATTTCCCGGTAACGCCGCTGGAGCTGCACGTGGGTAACAGCCATGATGTGATCAACGCCG
TGGCGGCGTTCAACGCCGATCTGGGACTGATCGAGGGCCCGTGTCACATGCCGGAGCTGACCACCCAAAGCTGGCTGGAG
GATGAGCTGGTGGTGTTTGCCGCACCGGACCATCCACTGTTGCGGGAGCCGGTTACCCTGGCCCGTCTGGCGGATGCGCC
GTGGATCCTGCGTGAGACCGGTTCCGGTACCCGCGAGGTGGTTGAGCATCTGTTGCTCTCCCATTTACCCCATTTTGATC
TGGTGATGGAGCTGGGCAACTCCGAGGCTATCAAGCATGCGGTGCGCTATGGTATCGGCATCAGCTGCCTGTCGCGCCGG
GTGATCGAAGAGCAGCTGAGCAATGGTACCCTGGTCGAGGTCGCGCTGCCGGAGATCAACCTGCATCGCTCGCTGTATCT
GATCCACCACCGCCAGAAGCATATTTCCAAGGCACTGGCCCGTTTTCTGAGCTACTGCCAGGCGTAG

Upstream 100 bases:

>100_bases
GTAAATGAAATTTGACGCAGAGAGCGGCCCCGTCGTCGTCGGGTCACAGGGCTATCGGCGCCGGGGACGGCGCGTTGGTT
GAAACGGTTTGGATCTGTCT

Downstream 100 bases:

>100_bases
CGCCTACGAGGCGGCAGCAGAGGCTGCTGCCGTAGTTTATTGTGATCTTGGCGTATTTTTATTACATACGCGATTAGCTA
CCCGCTAATAATCCAGCAAC

Product: DNA-binding transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 288; Mature: 288

Protein sequence:

>288_residues
MHITLRQLEVFTEVLKSGSTTQASIMLALSQSAVSAALSDLETQLSVRLFDRVGKRLVVNEHGRLLYPKAMALLEQASEI
EQLFKGESGALRIAASSTIGNYLLPPMMAAYRRDFPVTPLELHVGNSHDVINAVAAFNADLGLIEGPCHMPELTTQSWLE
DELVVFAAPDHPLLREPVTLARLADAPWILRETGSGTREVVEHLLLSHLPHFDLVMELGNSEAIKHAVRYGIGISCLSRR
VIEEQLSNGTLVEVALPEINLHRSLYLIHHRQKHISKALARFLSYCQA

Sequences:

>Translated_288_residues
MHITLRQLEVFTEVLKSGSTTQASIMLALSQSAVSAALSDLETQLSVRLFDRVGKRLVVNEHGRLLYPKAMALLEQASEI
EQLFKGESGALRIAASSTIGNYLLPPMMAAYRRDFPVTPLELHVGNSHDVINAVAAFNADLGLIEGPCHMPELTTQSWLE
DELVVFAAPDHPLLREPVTLARLADAPWILRETGSGTREVVEHLLLSHLPHFDLVMELGNSEAIKHAVRYGIGISCLSRR
VIEEQLSNGTLVEVALPEINLHRSLYLIHHRQKHISKALARFLSYCQA
>Mature_288_residues
MHITLRQLEVFTEVLKSGSTTQASIMLALSQSAVSAALSDLETQLSVRLFDRVGKRLVVNEHGRLLYPKAMALLEQASEI
EQLFKGESGALRIAASSTIGNYLLPPMMAAYRRDFPVTPLELHVGNSHDVINAVAAFNADLGLIEGPCHMPELTTQSWLE
DELVVFAAPDHPLLREPVTLARLADAPWILRETGSGTREVVEHLLLSHLPHFDLVMELGNSEAIKHAVRYGIGISCLSRR
VIEEQLSNGTLVEVALPEINLHRSLYLIHHRQKHISKALARFLSYCQA

Specific function: Unknown

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1788481, Length=286, Percent_Identity=73.7762237762238, Blast_Score=432, Evalue=1e-123,
Organism=Escherichia coli, GI87082132, Length=286, Percent_Identity=26.9230769230769, Blast_Score=100, Evalue=2e-22,
Organism=Escherichia coli, GI1788748, Length=283, Percent_Identity=27.208480565371, Blast_Score=96, Evalue=4e-21,
Organism=Escherichia coli, GI1788296, Length=244, Percent_Identity=30.327868852459, Blast_Score=93, Evalue=2e-20,
Organism=Escherichia coli, GI157672245, Length=229, Percent_Identity=29.2576419213974, Blast_Score=92, Evalue=5e-20,
Organism=Escherichia coli, GI1787879, Length=284, Percent_Identity=27.8169014084507, Blast_Score=79, Evalue=4e-16,
Organism=Escherichia coli, GI1786401, Length=307, Percent_Identity=22.8013029315961, Blast_Score=76, Evalue=2e-15,
Organism=Escherichia coli, GI1787601, Length=197, Percent_Identity=25.8883248730964, Blast_Score=75, Evalue=4e-15,
Organism=Escherichia coli, GI1787530, Length=293, Percent_Identity=23.8907849829352, Blast_Score=74, Evalue=1e-14,
Organism=Escherichia coli, GI1789204, Length=302, Percent_Identity=25.4966887417219, Blast_Score=71, Evalue=1e-13,
Organism=Escherichia coli, GI145693105, Length=285, Percent_Identity=21.4035087719298, Blast_Score=67, Evalue=1e-12,
Organism=Escherichia coli, GI1789639, Length=256, Percent_Identity=25.390625, Blast_Score=66, Evalue=3e-12,
Organism=Escherichia coli, GI1790208, Length=248, Percent_Identity=26.2096774193548, Blast_Score=65, Evalue=6e-12,
Organism=Escherichia coli, GI87081904, Length=214, Percent_Identity=26.1682242990654, Blast_Score=63, Evalue=2e-11,
Organism=Escherichia coli, GI1790399, Length=247, Percent_Identity=24.6963562753036, Blast_Score=63, Evalue=3e-11,
Organism=Escherichia coli, GI1788297, Length=241, Percent_Identity=24.0663900414938, Blast_Score=62, Evalue=3e-11,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 31900; Mature: 31900

Theoretical pI: Translated: 6.51; Mature: 6.51

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHITLRQLEVFTEVLKSGSTTQASIMLALSQSAVSAALSDLETQLSVRLFDRVGKRLVVN
CCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEE
EHGRLLYPKAMALLEQASEIEQLFKGESGALRIAASSTIGNYLLPPMMAAYRRDFPVTPL
CCCCEECHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCHHHHHHHHHHHCCCCCCCE
ELHVGNSHDVINAVAAFNADLGLIEGPCHMPELTTQSWLEDELVVFAAPDHPLLREPVTL
EEEECCCHHHHHHHHHHCCCCCCEECCCCCCCCCHHHHCCCCEEEEECCCCCHHHCCHHH
ARLADAPWILRETGSGTREVVEHLLLSHLPHFDLVMELGNSEAIKHAVRYGIGISCLSRR
HHHCCCCEEEEECCCCHHHHHHHHHHHHCCCHHHHHHCCCHHHHHHHHHHCCCHHHHHHH
VIEEQLSNGTLVEVALPEINLHRSLYLIHHRQKHISKALARFLSYCQA
HHHHHHCCCCEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MHITLRQLEVFTEVLKSGSTTQASIMLALSQSAVSAALSDLETQLSVRLFDRVGKRLVVN
CCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEE
EHGRLLYPKAMALLEQASEIEQLFKGESGALRIAASSTIGNYLLPPMMAAYRRDFPVTPL
CCCCEECHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCHHHHHHHHHHHCCCCCCCE
ELHVGNSHDVINAVAAFNADLGLIEGPCHMPELTTQSWLEDELVVFAAPDHPLLREPVTL
EEEECCCHHHHHHHHHHCCCCCCEECCCCCCCCCHHHHCCCCEEEEECCCCCHHHCCHHH
ARLADAPWILRETGSGTREVVEHLLLSHLPHFDLVMELGNSEAIKHAVRYGIGISCLSRR
HHHCCCCEEEEECCCCHHHHHHHHHHHHCCCHHHHHHCCCHHHHHHHHHHCCCHHHHHHH
VIEEQLSNGTLVEVALPEINLHRSLYLIHHRQKHISKALARFLSYCQA
HHHHHHCCCCEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]