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The map label for this gene is dhaK [H]

Identifier: 238919157

GI number: 238919157

Start: 1222641

End: 1223174

Strand: Direct

Name: dhaK [H]

Synonym: NT01EI_1228

Alternate gene names: 238919157

Gene position: 1222641-1223174 (Clockwise)

Preceding gene: 238919156

Following gene: 238919158

Centisome position: 32.07

GC content: 60.49

Gene sequence:

>534_bases
ATGTGGAAGGTCGGCGCCGCCTCTGCGGCGCGCGGCGATGAGATGGATCGTGTGATCGCCTATGCGCAGCGGGCCATCGA
TCGCTGTCGCTCCATCGGCATTGGCTTGAGCGCGTGTACGCTCCCGGCATTGGGAAAGCCCAATTTCTCTATTGCGGATG
GCTCGATGGAGGTGGGTATCGGCCATCACGGTGAGCCTGGGATCCGGGTCGAACCGCTGCGTCCCGCCGCGGAGATGGCG
GAGACGATGCTGCACGCTGTCATGGCGGAGCTGGCGCTGGTCCCCGGGAGTGAGGTGGCGGTATTGGTCTCCGGGCTGGG
GGCGACGCCGGTGATGGAACTGTATATCTACTATGATGCCGTAGCGCGTCGTCTGGAGGCGCAGGGGATAGCGATACACC
GTTGCTACGTTGGAAACTATTTTACCTCGCTGGATATGATGGGGGTAACGCTGACGGTTATGGCGTTGGACGATGAGCTG
AAATTATTACTCAATATGCCGGTGTCATCACCGGGATTGACGCAGGAGGGGTAA

Upstream 100 bases:

>100_bases
GAGGGGATCGTGGTTAAAACGGTGGGTAGCCAATGACGATGTGGCCTCTGCGCCGGAGAGTGAGCGAGAAAAGCGTCGTG
GCGTCGCGGGGGAAATATTT

Downstream 100 bases:

>100_bases
TCACGATGGCTTATGCAGTCACCGTTTCCAACGGCGGAGAAATTGTCGCTGCGCTGGTCGCCGCGGTGGTCAGCCAGCGG
ACGTATCTCAGTGAAATTGA

Product: PTS-dependent dihydroxyacetone kinase, dihydroxyacetone-bindingsubunit DhaK

Products: ADP; glycerone phosphate

Alternate protein names: NA

Number of amino acids: Translated: 177; Mature: 177

Protein sequence:

>177_residues
MWKVGAASAARGDEMDRVIAYAQRAIDRCRSIGIGLSACTLPALGKPNFSIADGSMEVGIGHHGEPGIRVEPLRPAAEMA
ETMLHAVMAELALVPGSEVAVLVSGLGATPVMELYIYYDAVARRLEAQGIAIHRCYVGNYFTSLDMMGVTLTVMALDDEL
KLLLNMPVSSPGLTQEG

Sequences:

>Translated_177_residues
MWKVGAASAARGDEMDRVIAYAQRAIDRCRSIGIGLSACTLPALGKPNFSIADGSMEVGIGHHGEPGIRVEPLRPAAEMA
ETMLHAVMAELALVPGSEVAVLVSGLGATPVMELYIYYDAVARRLEAQGIAIHRCYVGNYFTSLDMMGVTLTVMALDDEL
KLLLNMPVSSPGLTQEG
>Mature_177_residues
MWKVGAASAARGDEMDRVIAYAQRAIDRCRSIGIGLSACTLPALGKPNFSIADGSMEVGIGHHGEPGIRVEPLRPAAEMA
ETMLHAVMAELALVPGSEVAVLVSGLGATPVMELYIYYDAVARRLEAQGIAIHRCYVGNYFTSLDMMGVTLTVMALDDEL
KLLLNMPVSSPGLTQEG

Specific function: Dihydroxyacetone binding subunit of the dihydroxyacetone kinase, which is responsible for phosphorylating dihydroxyacetone. Binds covalently dihydroxyacetone in hemiaminal linkage. Acts also as a corepressor of dhaR by binding to its sensor domain, in the

COG id: COG2376

COG function: function code G; Dihydroxyacetone kinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 DhaK domain [H]

Homologues:

Organism=Homo sapiens, GI20149621, Length=140, Percent_Identity=34.2857142857143, Blast_Score=91, Evalue=7e-19,
Organism=Escherichia coli, GI87081857, Length=196, Percent_Identity=37.7551020408163, Blast_Score=129, Evalue=1e-31,
Organism=Caenorhabditis elegans, GI17565018, Length=170, Percent_Identity=30, Blast_Score=92, Evalue=1e-19,
Organism=Saccharomyces cerevisiae, GI6321055, Length=185, Percent_Identity=27.027027027027, Blast_Score=61, Evalue=9e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004006
- InterPro:   IPR012736 [H]

Pfam domain/function: PF02733 Dak1 [H]

EC number: 2.7.1.29

Molecular weight: Translated: 18805; Mature: 18805

Theoretical pI: Translated: 4.78; Mature: 4.78

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
6.2 %Met     (Translated Protein)
7.9 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
6.2 %Met     (Mature Protein)
7.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MWKVGAASAARGDEMDRVIAYAQRAIDRCRSIGIGLSACTLPALGKPNFSIADGSMEVGI
CCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHCCCCCCCCCCEEECCCEEECC
GHHGEPGIRVEPLRPAAEMAETMLHAVMAELALVPGSEVAVLVSGLGATPVMELYIYYDA
CCCCCCCCEECCCCCHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCHHHHHHHHHHH
VARRLEAQGIAIHRCYVGNYFTSLDMMGVTLTVMALDDELKLLLNMPVSSPGLTQEG
HHHHHHHCCEEEEEEECCCHHHHHHHHCEEEEEEEECHHHEEEEECCCCCCCCCCCC
>Mature Secondary Structure
MWKVGAASAARGDEMDRVIAYAQRAIDRCRSIGIGLSACTLPALGKPNFSIADGSMEVGI
CCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHCCCCCCCCCCEEECCCEEECC
GHHGEPGIRVEPLRPAAEMAETMLHAVMAELALVPGSEVAVLVSGLGATPVMELYIYYDA
CCCCCCCCEECCCCCHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCHHHHHHHHHHH
VARRLEAQGIAIHRCYVGNYFTSLDMMGVTLTVMALDDELKLLLNMPVSSPGLTQEG
HHHHHHHCCEEEEEEECCCHHHHHHHHCEEEEEEEECHHHEEEEECCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; glycerone

Specific reaction: ATP + glycerone = ADP + glycerone phosphate

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8905232; 9278503 [H]