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The map label for this gene is phoB [H]

Identifier: 238919113

GI number: 238919113

Start: 1177425

End: 1178114

Strand: Direct

Name: phoB [H]

Synonym: NT01EI_1184

Alternate gene names: 238919113

Gene position: 1177425-1178114 (Clockwise)

Preceding gene: 238919112

Following gene: 238919114

Centisome position: 30.88

GC content: 57.83

Gene sequence:

>690_bases
ATGACCATACGCATTTTAGTCGTAGAAGATGAAACGCCCATCAGGGATATGGTGAGTTTCGTTCTGGAGCAGAACGGCTA
TCAGCCGCTGGAGGCGGAGAGCTATGACGGTGCGTTAAGCCAGCTGTGCGAGCCTTACCCCGATCTGATTCTGCTGGACT
GGATGCTGCCCGGCGGCTCGGGTATCCAGCTTATCAAGCAGCTGAAGCGGGAACCGGCGACCCGGGATATTCCGGTGATG
ATGCTGACGGCGCGCGGTGAAGAGGAGGATCGGGTGCGCGGTCTGGAGGTGGGCGCCGATGATTATATTACCAAGCCGTT
TTCGCCCAAAGAGCTGGTGGCGCGGATCAAGGCAGTAATGCGGCGCATTTCGCCGATGGCGCTGGAGGAGAGCATTAGCC
TGCAGGGGCTGAGCCTAGACCCTATCTCGCACCGGGTGACGGTCCACGATAGTGCGGTGGATATGGGTCCGACCGAATTT
AAACTGCTGCACTTCTTTATGACTCATCCTGAGCGGGTTTACAGCCGCGAACAGTTGCTTAATCATGTCTGGGGCACTAA
CGTCTATGTGGAGGATCGTACCGTCGATGTTCACATTCGTCGTTTGCGCAAGGCGCTGGAGGGCAGCGGTCACGATCGCA
TGGTGCAGACCGTGCGTGGTACCGGCTACCGTTTCTCCGCCCGCTACTGA

Upstream 100 bases:

>100_bases
CCCGCTGGCCGCCGCGGCTGGGGGGATCTCTTTTTTTTCATAAATCTGTCATAAAAATGACGCATAATTGCAGCGGATGA
CAATTAACCGGGAAGTCAGT

Downstream 100 bases:

>100_bases
TCGGCGATGGAGTGCTGACTGTGTTGGATAAACTCTCCTGGAAGACGCTGCTGTCTGAGCTGCTGTTGTGCTGCGTGCCG
GCGCTGCTGCTCGGCACGCT

Product: phosphate regulon transcriptional regulatory protein PhoB

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 229; Mature: 228

Protein sequence:

>229_residues
MTIRILVVEDETPIRDMVSFVLEQNGYQPLEAESYDGALSQLCEPYPDLILLDWMLPGGSGIQLIKQLKREPATRDIPVM
MLTARGEEEDRVRGLEVGADDYITKPFSPKELVARIKAVMRRISPMALEESISLQGLSLDPISHRVTVHDSAVDMGPTEF
KLLHFFMTHPERVYSREQLLNHVWGTNVYVEDRTVDVHIRRLRKALEGSGHDRMVQTVRGTGYRFSARY

Sequences:

>Translated_229_residues
MTIRILVVEDETPIRDMVSFVLEQNGYQPLEAESYDGALSQLCEPYPDLILLDWMLPGGSGIQLIKQLKREPATRDIPVM
MLTARGEEEDRVRGLEVGADDYITKPFSPKELVARIKAVMRRISPMALEESISLQGLSLDPISHRVTVHDSAVDMGPTEF
KLLHFFMTHPERVYSREQLLNHVWGTNVYVEDRTVDVHIRRLRKALEGSGHDRMVQTVRGTGYRFSARY
>Mature_228_residues
TIRILVVEDETPIRDMVSFVLEQNGYQPLEAESYDGALSQLCEPYPDLILLDWMLPGGSGIQLIKQLKREPATRDIPVMM
LTARGEEEDRVRGLEVGADDYITKPFSPKELVARIKAVMRRISPMALEESISLQGLSLDPISHRVTVHDSAVDMGPTEFK
LLHFFMTHPERVYSREQLLNHVWGTNVYVEDRTVDVHIRRLRKALEGSGHDRMVQTVRGTGYRFSARY

Specific function: This protein is a positive regulator for the phosphate regulon. Transcription of this operon is positively regulated by phoB and phoR when phosphate is limited [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 response regulatory domain [H]

Homologues:

Organism=Escherichia coli, GI1786599, Length=229, Percent_Identity=84.2794759825327, Blast_Score=399, Evalue=1e-113,
Organism=Escherichia coli, GI1788394, Length=228, Percent_Identity=41.2280701754386, Blast_Score=169, Evalue=2e-43,
Organism=Escherichia coli, GI87082012, Length=226, Percent_Identity=36.283185840708, Blast_Score=143, Evalue=8e-36,
Organism=Escherichia coli, GI1786784, Length=222, Percent_Identity=34.2342342342342, Blast_Score=141, Evalue=4e-35,
Organism=Escherichia coli, GI1789809, Length=229, Percent_Identity=35.8078602620087, Blast_Score=141, Evalue=4e-35,
Organism=Escherichia coli, GI1790860, Length=222, Percent_Identity=37.8378378378378, Blast_Score=140, Evalue=6e-35,
Organism=Escherichia coli, GI1786911, Length=223, Percent_Identity=35.8744394618834, Blast_Score=138, Evalue=4e-34,
Organism=Escherichia coli, GI1790863, Length=237, Percent_Identity=35.8649789029536, Blast_Score=132, Evalue=1e-32,
Organism=Escherichia coli, GI1787375, Length=226, Percent_Identity=32.3008849557522, Blast_Score=125, Evalue=2e-30,
Organism=Escherichia coli, GI2367329, Length=229, Percent_Identity=36.2445414847162, Blast_Score=119, Evalue=1e-28,
Organism=Escherichia coli, GI1789402, Length=227, Percent_Identity=33.920704845815, Blast_Score=112, Evalue=3e-26,
Organism=Escherichia coli, GI1787229, Length=230, Percent_Identity=33.9130434782609, Blast_Score=107, Evalue=8e-25,
Organism=Escherichia coli, GI1790552, Length=225, Percent_Identity=31.5555555555556, Blast_Score=103, Evalue=1e-23,
Organism=Escherichia coli, GI145693140, Length=236, Percent_Identity=28.3898305084746, Blast_Score=97, Evalue=8e-22,
Organism=Escherichia coli, GI1788191, Length=124, Percent_Identity=28.2258064516129, Blast_Score=71, Evalue=6e-14,
Organism=Escherichia coli, GI1788550, Length=109, Percent_Identity=37.6146788990826, Blast_Score=70, Evalue=1e-13,
Organism=Escherichia coli, GI1790299, Length=147, Percent_Identity=29.2517006802721, Blast_Score=63, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011006
- InterPro:   IPR001867
- InterPro:   IPR011879
- InterPro:   IPR001789
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00072 Response_reg; PF00486 Trans_reg_C [H]

EC number: NA

Molecular weight: Translated: 26181; Mature: 26049

Theoretical pI: Translated: 5.91; Mature: 5.91

Prosite motif: PS50110 RESPONSE_REGULATORY

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
4.4 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
3.9 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTIRILVVEDETPIRDMVSFVLEQNGYQPLEAESYDGALSQLCEPYPDLILLDWMLPGGS
CEEEEEEEECCCCHHHHHHHHHHCCCCCCCCCCCCCHHHHHHCCCCCCEEEEEEECCCCC
GIQLIKQLKREPATRDIPVMMLTARGEEEDRVRGLEVGADDYITKPFSPKELVARIKAVM
HHHHHHHHHHCCCCCCCCEEEEEECCCHHHHHCCEEECCCCCCCCCCCHHHHHHHHHHHH
RRISPMALEESISLQGLSLDPISHRVTVHDSAVDMGPTEFKLLHFFMTHPERVYSREQLL
HHCCCHHHHHCCCCCCCEECCCCEEEEEECCCCCCCCHHHHHHHHHHCCHHHHHHHHHHH
NHVWGTNVYVEDRTVDVHIRRLRKALEGSGHDRMVQTVRGTGYRFSARY
HHHCCCCEEEECCCHHHHHHHHHHHHCCCCHHHHHHHHHCCCEEEECCC
>Mature Secondary Structure 
TIRILVVEDETPIRDMVSFVLEQNGYQPLEAESYDGALSQLCEPYPDLILLDWMLPGGS
EEEEEEEECCCCHHHHHHHHHHCCCCCCCCCCCCCHHHHHHCCCCCCEEEEEEECCCCC
GIQLIKQLKREPATRDIPVMMLTARGEEEDRVRGLEVGADDYITKPFSPKELVARIKAVM
HHHHHHHHHHCCCCCCCCEEEEEECCCHHHHHCCEEECCCCCCCCCCCHHHHHHHHHHHH
RRISPMALEESISLQGLSLDPISHRVTVHDSAVDMGPTEFKLLHFFMTHPERVYSREQLL
HHCCCHHHHHCCCCCCCEECCCCEEEEEECCCCCCCCHHHHHHHHHHCCHHHHHHHHHHH
NHVWGTNVYVEDRTVDVHIRRLRKALEGSGHDRMVQTVRGTGYRFSARY
HHHCCCCEEEECCCHHHHHHHHHHHHCCCCHHHHHHHHHCCCEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]