Click here to switch to the map view.

The map label for this gene is mak [H]

Identifier: 238919107

GI number: 238919107

Start: 1170693

End: 1171295

Strand: Direct

Name: mak [H]

Synonym: NT01EI_1178

Alternate gene names: 238919107

Gene position: 1170693-1171295 (Clockwise)

Preceding gene: 238919106

Following gene: 238919108

Centisome position: 30.71

GC content: 68.66

Gene sequence:

>603_bases
TTGGCCAACGATGCAAACTGTCTGGCGGTATCGGAGGCGACCGACGGTGCGGCGGCCGGTGCCCGGGTAGTCTTTGCCGT
GATTATCGGCACTGGCTGCGGCGCGGGGATTGCGCTGGATGGCCGGGTGCACGCCGGCGGTAACGGCATCGCCGGCGAGT
GGGGGCATAACCCCTTGCCCTGGCAGGATGACGCCGAACGGGCGGCGTCAGCGACGGCGCCCTGCTACTGTGGCAAGCAC
GGCTGCATTGAAACCTTTGTCTCCGGCAGCGGATTCTGCGCCGACTACCGATGCCATGGCGGTGCGGTGCTTGACGGTGC
CCGGATCATGGCGCTGGCCGACGCGGGCGATGTGGCGGCCCTGGCGGCCATCGCCCGCTATGAACAGCGTCTGGCGCGTG
CGCTGGCGCAGACCATTAACACGCTGGATCCGGACGTGATCGTGCTGGGCGGCGGCATGAGCAACGTGAGCCGTCTGTAT
CGCACCCTGCCGGATCTGATCACCCCCTGGGTATTCGGCGGTGAGTGCCGTACCCCGATTCGTCCGGCGCAGCACGGCGA
CGCCAGCGGGGTACGCGGCGCCGCGTGGCTGTGGCCCGCGTAA

Upstream 100 bases:

>100_bases
TCCCCCGTTTAGCGGGCGGGTAAAGAATGCCAACTCGACCTGGCTGAACGGCTCGACGCTGGACGCCGACCTCTCTGCCC
TGCTGAAAAGGGAGGTGCGG

Downstream 100 bases:

>100_bases
CGGCGGGATGCCCGGAAATTTGCCATACTGGCAGAGGCGCCGACATGGCGCGCGGGGAGGGGAGTCGAGGGTTATGCAGG
TCAGGATCGTGAGTCTGGCG

Product: N-acetyl-D-glucosamine kinase

Products: NA

Alternate protein names: D-fructose kinase; Manno(fructo)kinase [H]

Number of amino acids: Translated: 200; Mature: 199

Protein sequence:

>200_residues
MANDANCLAVSEATDGAAAGARVVFAVIIGTGCGAGIALDGRVHAGGNGIAGEWGHNPLPWQDDAERAASATAPCYCGKH
GCIETFVSGSGFCADYRCHGGAVLDGARIMALADAGDVAALAAIARYEQRLARALAQTINTLDPDVIVLGGGMSNVSRLY
RTLPDLITPWVFGGECRTPIRPAQHGDASGVRGAAWLWPA

Sequences:

>Translated_200_residues
MANDANCLAVSEATDGAAAGARVVFAVIIGTGCGAGIALDGRVHAGGNGIAGEWGHNPLPWQDDAERAASATAPCYCGKH
GCIETFVSGSGFCADYRCHGGAVLDGARIMALADAGDVAALAAIARYEQRLARALAQTINTLDPDVIVLGGGMSNVSRLY
RTLPDLITPWVFGGECRTPIRPAQHGDASGVRGAAWLWPA
>Mature_199_residues
ANDANCLAVSEATDGAAAGARVVFAVIIGTGCGAGIALDGRVHAGGNGIAGEWGHNPLPWQDDAERAASATAPCYCGKHG
CIETFVSGSGFCADYRCHGGAVLDGARIMALADAGDVAALAAIARYEQRLARALAQTINTLDPDVIVLGGGMSNVSRLYR
TLPDLITPWVFGGECRTPIRPAQHGDASGVRGAAWLWPA

Specific function: Catalyzes the phosphorylation of fructose to fructose-6- P. Has also low level glucokinase activity in vitro. Is not able to phosphorylate D-ribose, D-mannitol, D-sorbitol, inositol, and L-threonine [H]

COG id: COG1940

COG function: function code KG; Transcriptional regulator/sugar kinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ROK (nagC/xylR) family [H]

Homologues:

Organism=Escherichia coli, GI87081733, Length=199, Percent_Identity=67.3366834170854, Blast_Score=285, Evalue=2e-78,
Organism=Escherichia coli, GI1787363, Length=201, Percent_Identity=40.7960199004975, Blast_Score=132, Evalue=2e-32,
Organism=Escherichia coli, GI1790522, Length=183, Percent_Identity=31.6939890710383, Blast_Score=63, Evalue=2e-11,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000600 [H]

Pfam domain/function: PF00480 ROK [H]

EC number: =2.7.1.4 [H]

Molecular weight: Translated: 20460; Mature: 20329

Theoretical pI: Translated: 5.54; Mature: 5.54

Prosite motif: PS01125 ROK

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

4.0 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
5.5 %Cys+Met (Translated Protein)
4.0 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MANDANCLAVSEATDGAAAGARVVFAVIIGTGCGAGIALDGRVHAGGNGIAGEWGHNPLP
CCCCCCEEEEECCCCCCHHHHHHHHEEEHHCCCCCCEEECCEEECCCCCCCCCCCCCCCC
WQDDAERAASATAPCYCGKHGCIETFVSGSGFCADYRCHGGAVLDGARIMALADAGDVAA
CCCCHHHHHCCCCCCCCCCCHHHHHHHCCCCCEEEEEECCCEEECCCEEEEEECCCHHHH
LAAIARYEQRLARALAQTINTLDPDVIVLGGGMSNVSRLYRTLPDLITPWVFGGECRTPI
HHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCHHHHHHHHHHHHHHHCCCEECCCCCCCC
RPAQHGDASGVRGAAWLWPA
CCCCCCCCCCCCCCEECCCC
>Mature Secondary Structure 
ANDANCLAVSEATDGAAAGARVVFAVIIGTGCGAGIALDGRVHAGGNGIAGEWGHNPLP
CCCCCEEEEECCCCCCHHHHHHHHEEEHHCCCCCCEEECCEEECCCCCCCCCCCCCCCC
WQDDAERAASATAPCYCGKHGCIETFVSGSGFCADYRCHGGAVLDGARIMALADAGDVAA
CCCCHHHHHCCCCCCCCCCCHHHHHHHCCCCCEEEEEECCCEEECCCEEEEEECCCHHHH
LAAIARYEQRLARALAQTINTLDPDVIVLGGGMSNVSRLYRTLPDLITPWVFGGECRTPI
HHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCHHHHHHHHHHHHHHHCCCEECCCCCCCC
RPAQHGDASGVRGAAWLWPA
CCCCCCCCCCCCCCEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8807285; 9278503; 1744033 [H]