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The map label for this gene is metG [H]

Identifier: 238917941

GI number: 238917941

Start: 2098244

End: 2100217

Strand: Reverse

Name: metG [H]

Synonym: EUBELI_02029

Alternate gene names: 238917941

Gene position: 2100217-2098244 (Counterclockwise)

Preceding gene: 238917943

Following gene: 238917940

Centisome position: 97.95

GC content: 39.67

Gene sequence:

>1974_bases
ATGCCACAGAGTAAAGGTCCATTTTATATGACAACAGCTATTGCCTATACATCAGGCAAGCCACATATCGGTAATACTTA
TGAAATCGTTCTTGCAGACAGTATTGCAAGATTCAGAAGACAGGAAGGCTATGATGTATTTTTCCAGACAGGAACTGACG
AGCATGGTCAGAAGATTGAGCTTAAGGCTGAAGAAGCTGGAATTACACCTAAGGAATTCGTTGATAATGTTTCAACAGAG
ATTAAGAGAATATGGGATCTTATGAATACTTCATATGATAAGTTCATCAGAACAACAGATGATTACCATGAGAAGCAGGT
TCAGAAGATTTTTAAGAAACTTTATGATCAGGGAGACATTTATAAGGGTTCATATGAAGGCATGTACTGTACTCCATGTG
AGTCTTTCTGGACAGAGAGCCAGCTTGTTGATGGAAAGTGTCCTGATTGTGGACGTGAAGTTAAGCCAGCTAAGGAAGAA
GCTTATTTCTTCAAGATGAGCAAATATGCCAACAAGCTTATTGAGCATATCAATACACATCCGGAATTCATCCAGCCTGT
TTCAAGAAAGAATGAGATGATGAACAACTTCCTTCTTCCTGGACTTCAGGATTTATGTGTATCAAGAACATCTTTCAAAT
GGGGTATTCCAGTTGACTTTGATGACAAGCATGTAGTTTATGTATGGCTTGATGCCCTTACTAACTATATCACAGGTATC
GGATATGATGCTGATGGCAATTCTTCAGAGCAGTACAAGAAGTTCTGGCCAGCAGACCTTCACCTTATCGGTAAGGATAT
CATCAGATTCCATACAATTTACTGGCCAATATTCCTTATGGCACTTGGTGAGCCACTTCCTAAGCAGGTGTTTGGACATC
CTTGGTTATTACAGAATGATGGTAAGATGAGTAAGTCTAAGGGAAATGTTATCTATGCAGATGATCTTGTAGACCTTTTC
GGCGTTGATGCTGTACGTTACTTTGTTCTTCACGAGATGCCATTTGAGAATGATGGTGTTATTTCATGGGAGCTTATGGT
TGAGCGTATGAACTCAGACCTTGCCAACACACTTGGTAACCTTGTAAACAGAACAATTGCAATGTCTAACAAGTACTTTG
GTGGAGTTGTTAATAAGACTGGCGTAGAGGATGCTGCAATTGACGGTGACCTTAAGGCTGTTGTAACAGCAACAAGAGAT
AAGGTTCAGGCAAAGATGGCTACACTTCATGTAGCAGATGCTATCACAGAAGTATTCACACTCTTTAAGAGATGTAACAA
ATATATTGACGAGACAATGCCTTGGGCTCTTGCAAAGGATGAGGCACAGCAGGACAGATTAGCAGAAGTGCTCTATAACC
TTGTTGAGAGTATTACAATCGGTGCTAATCTTCTTAAGTCTTTCATGCCAGAGACTACAGATAAGATTCTTGCACAGCTT
TACCCAGCTAATCCAGAGGCAGGAGTAAGAGATTTTGATGACCTTGCTACATTTGGCTTAAGAGAAACAGGACTTAAGGT
TACTGAGACACCAGAGATTCTTTTCGCAAGACTTGACTTTGAGAAGGACCTTAAGGAAAAGGTTGATGCTATTCAGGAAG
CTCAGAAGAAGGCTAATGGTGTTACAGAATATCCACAGGTTGAAGTTAAGCCTGAGATTACATTTGATGATTTTGAAAAG
GTTCAGTTCAGAGTTGCCAAGGTTCTTACATGTGAAGCTGTTAAGAAGACAAAGCTTCTTAAGTTCGAGCTTCAGATTGG
TGATGAGAAGAGAACTATCGTATCAGGTATCCAGAAGTACTATAAGCCAGAGGAGCTTATTGGTAAGAAGTTAGTTGTAT
GTACTAATTTAAAACCAAGAAAGATCTGTGGTATTGAGTCACAGGGAATGATTATCTCAGCATGGGATGATAAGGATAAC
TTATCAGTTCTTACACTTGAGAAAGATATTATTGAAGGTGCAGAGATTGGCTGA

Upstream 100 bases:

>100_bases
TTATTGTAAATTTTTCTATGATAAAGTTTACGGATAAATGATTCACACTACATTTGCGTTATAGCGATGAATTAAAATAA
TAGATTATGGAGGAAATGAT

Downstream 100 bases:

>100_bases
TAGATATGAAGATATTTGAAACTCATGCTCATTATGATGATGAAAGATTTGATGAGGACAGAGATGAACTTATAAGCAGC
ATGCTTAGTGATACAGGGGA

Product: methionyl-tRNA synthetase

Products: NA

Alternate protein names: Methionine--tRNA ligase; MetRS [H]

Number of amino acids: Translated: 657; Mature: 656

Protein sequence:

>657_residues
MPQSKGPFYMTTAIAYTSGKPHIGNTYEIVLADSIARFRRQEGYDVFFQTGTDEHGQKIELKAEEAGITPKEFVDNVSTE
IKRIWDLMNTSYDKFIRTTDDYHEKQVQKIFKKLYDQGDIYKGSYEGMYCTPCESFWTESQLVDGKCPDCGREVKPAKEE
AYFFKMSKYANKLIEHINTHPEFIQPVSRKNEMMNNFLLPGLQDLCVSRTSFKWGIPVDFDDKHVVYVWLDALTNYITGI
GYDADGNSSEQYKKFWPADLHLIGKDIIRFHTIYWPIFLMALGEPLPKQVFGHPWLLQNDGKMSKSKGNVIYADDLVDLF
GVDAVRYFVLHEMPFENDGVISWELMVERMNSDLANTLGNLVNRTIAMSNKYFGGVVNKTGVEDAAIDGDLKAVVTATRD
KVQAKMATLHVADAITEVFTLFKRCNKYIDETMPWALAKDEAQQDRLAEVLYNLVESITIGANLLKSFMPETTDKILAQL
YPANPEAGVRDFDDLATFGLRETGLKVTETPEILFARLDFEKDLKEKVDAIQEAQKKANGVTEYPQVEVKPEITFDDFEK
VQFRVAKVLTCEAVKKTKLLKFELQIGDEKRTIVSGIQKYYKPEELIGKKLVVCTNLKPRKICGIESQGMIISAWDDKDN
LSVLTLEKDIIEGAEIG

Sequences:

>Translated_657_residues
MPQSKGPFYMTTAIAYTSGKPHIGNTYEIVLADSIARFRRQEGYDVFFQTGTDEHGQKIELKAEEAGITPKEFVDNVSTE
IKRIWDLMNTSYDKFIRTTDDYHEKQVQKIFKKLYDQGDIYKGSYEGMYCTPCESFWTESQLVDGKCPDCGREVKPAKEE
AYFFKMSKYANKLIEHINTHPEFIQPVSRKNEMMNNFLLPGLQDLCVSRTSFKWGIPVDFDDKHVVYVWLDALTNYITGI
GYDADGNSSEQYKKFWPADLHLIGKDIIRFHTIYWPIFLMALGEPLPKQVFGHPWLLQNDGKMSKSKGNVIYADDLVDLF
GVDAVRYFVLHEMPFENDGVISWELMVERMNSDLANTLGNLVNRTIAMSNKYFGGVVNKTGVEDAAIDGDLKAVVTATRD
KVQAKMATLHVADAITEVFTLFKRCNKYIDETMPWALAKDEAQQDRLAEVLYNLVESITIGANLLKSFMPETTDKILAQL
YPANPEAGVRDFDDLATFGLRETGLKVTETPEILFARLDFEKDLKEKVDAIQEAQKKANGVTEYPQVEVKPEITFDDFEK
VQFRVAKVLTCEAVKKTKLLKFELQIGDEKRTIVSGIQKYYKPEELIGKKLVVCTNLKPRKICGIESQGMIISAWDDKDN
LSVLTLEKDIIEGAEIG
>Mature_656_residues
PQSKGPFYMTTAIAYTSGKPHIGNTYEIVLADSIARFRRQEGYDVFFQTGTDEHGQKIELKAEEAGITPKEFVDNVSTEI
KRIWDLMNTSYDKFIRTTDDYHEKQVQKIFKKLYDQGDIYKGSYEGMYCTPCESFWTESQLVDGKCPDCGREVKPAKEEA
YFFKMSKYANKLIEHINTHPEFIQPVSRKNEMMNNFLLPGLQDLCVSRTSFKWGIPVDFDDKHVVYVWLDALTNYITGIG
YDADGNSSEQYKKFWPADLHLIGKDIIRFHTIYWPIFLMALGEPLPKQVFGHPWLLQNDGKMSKSKGNVIYADDLVDLFG
VDAVRYFVLHEMPFENDGVISWELMVERMNSDLANTLGNLVNRTIAMSNKYFGGVVNKTGVEDAAIDGDLKAVVTATRDK
VQAKMATLHVADAITEVFTLFKRCNKYIDETMPWALAKDEAQQDRLAEVLYNLVESITIGANLLKSFMPETTDKILAQLY
PANPEAGVRDFDDLATFGLRETGLKVTETPEILFARLDFEKDLKEKVDAIQEAQKKANGVTEYPQVEVKPEITFDDFEKV
QFRVAKVLTCEAVKKTKLLKFELQIGDEKRTIVSGIQKYYKPEELIGKKLVVCTNLKPRKICGIESQGMIISAWDDKDNL
SVLTLEKDIIEGAEIG

Specific function: Is required not only for elongation of protein synthesis but also for the initiation of all mRNA translation through initiator tRNA(fMet) aminoacylation [H]

COG id: COG0143

COG function: function code J; Methionyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 tRNA-binding domain [H]

Homologues:

Organism=Homo sapiens, GI24308436, Length=549, Percent_Identity=33.5154826958106, Blast_Score=279, Evalue=6e-75,
Organism=Homo sapiens, GI14043022, Length=534, Percent_Identity=28.6516853932584, Blast_Score=197, Evalue=2e-50,
Organism=Escherichia coli, GI1788432, Length=698, Percent_Identity=27.0773638968481, Blast_Score=250, Evalue=2e-67,
Organism=Escherichia coli, GI1786861, Length=194, Percent_Identity=25.7731958762887, Blast_Score=77, Evalue=5e-15,
Organism=Caenorhabditis elegans, GI212645096, Length=493, Percent_Identity=32.8600405679513, Blast_Score=247, Evalue=2e-65,
Organism=Caenorhabditis elegans, GI17540794, Length=606, Percent_Identity=27.3927392739274, Blast_Score=193, Evalue=2e-49,
Organism=Caenorhabditis elegans, GI71992781, Length=383, Percent_Identity=31.0704960835509, Blast_Score=173, Evalue=3e-43,
Organism=Caenorhabditis elegans, GI71997510, Length=182, Percent_Identity=27.4725274725275, Blast_Score=66, Evalue=6e-11,
Organism=Caenorhabditis elegans, GI71997517, Length=182, Percent_Identity=27.4725274725275, Blast_Score=65, Evalue=8e-11,
Organism=Saccharomyces cerevisiae, GI6321610, Length=518, Percent_Identity=31.6602316602317, Blast_Score=257, Evalue=5e-69,
Organism=Saccharomyces cerevisiae, GI6321704, Length=558, Percent_Identity=26.1648745519713, Blast_Score=171, Evalue=4e-43,
Organism=Saccharomyces cerevisiae, GI6323414, Length=179, Percent_Identity=30.1675977653631, Blast_Score=74, Evalue=6e-14,
Organism=Drosophila melanogaster, GI21358483, Length=546, Percent_Identity=29.8534798534799, Blast_Score=249, Evalue=5e-66,
Organism=Drosophila melanogaster, GI24655311, Length=622, Percent_Identity=27.8135048231511, Blast_Score=195, Evalue=9e-50,

Paralogues:

None

Copy number: 871 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015413
- InterPro:   IPR004495
- InterPro:   IPR014758
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR014729
- InterPro:   IPR002547
- InterPro:   IPR009080
- InterPro:   IPR013155 [H]

Pfam domain/function: PF08264 Anticodon_1; PF09334 tRNA-synt_1g; PF01588 tRNA_bind [H]

EC number: =6.1.1.10 [H]

Molecular weight: Translated: 74820; Mature: 74689

Theoretical pI: Translated: 5.00; Mature: 5.00

Prosite motif: PS50886 TRBD

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPQSKGPFYMTTAIAYTSGKPHIGNTYEIVLADSIARFRRQEGYDVFFQTGTDEHGQKIE
CCCCCCCEEEEEEEEEECCCCCCCCEEEEEEHHHHHHHHHCCCCEEEEECCCCCCCCEEE
LKAEEAGITPKEFVDNVSTEIKRIWDLMNTSYDKFIRTTDDYHEKQVQKIFKKLYDQGDI
EEHHHCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHCCCCHHHHHHHHHHHHHHCCCCCE
YKGSYEGMYCTPCESFWTESQLVDGKCPDCGREVKPAKEEAYFFKMSKYANKLIEHINTH
ECCCCCCEEECCCHHHCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCC
PEFIQPVSRKNEMMNNFLLPGLQDLCVSRTSFKWGIPVDFDDKHVVYVWLDALTNYITGI
HHHHHHHHHHHHHHHHHCCCCHHHHHCCCCCCCCCCCCCCCCCEEEEEEHHHHHHHHHCC
GYDADGNSSEQYKKFWPADLHLIGKDIIRFHTIYWPIFLMALGEPLPKQVFGHPWLLQND
CCCCCCCCHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHCCCCCEEECC
GKMSKSKGNVIYADDLVDLFGVDAVRYFVLHEMPFENDGVISWELMVERMNSDLANTLGN
CCCCCCCCCEEEECHHHHHHHHHHHHHHHHHCCCCCCCCEEEHHHHHHHHHHHHHHHHHH
LVNRTIAMSNKYFGGVVNKTGVEDAAIDGDLKAVVTATRDKVQAKMATLHVADAITEVFT
HHHHHHHHCCCCCCCCCCCCCCCHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LFKRCNKYIDETMPWALAKDEAQQDRLAEVLYNLVESITIGANLLKSFMPETTDKILAQL
HHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHH
YPANPEAGVRDFDDLATFGLRETGLKVTETPEILFARLDFEKDLKEKVDAIQEAQKKANG
CCCCCCCCCCCHHHHHHHCHHHCCCEEECCHHHEEEECCCHHHHHHHHHHHHHHHHHHCC
VTEYPQVEVKPEITFDDFEKVQFRVAKVLTCEAVKKTKLLKFELQIGDEKRTIVSGIQKY
CCCCCCEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHEEEEEEEECCCHHHHHHHHHHH
YKPEELIGKKLVVCTNLKPRKICGIESQGMIISAWDDKDNLSVLTLEKDIIEGAEIG
CCCHHHHCCEEEEEECCCCHHCCCCCCCCEEEEECCCCCCCEEEEEEHHHHCCCCCC
>Mature Secondary Structure 
PQSKGPFYMTTAIAYTSGKPHIGNTYEIVLADSIARFRRQEGYDVFFQTGTDEHGQKIE
CCCCCCEEEEEEEEEECCCCCCCCEEEEEEHHHHHHHHHCCCCEEEEECCCCCCCCEEE
LKAEEAGITPKEFVDNVSTEIKRIWDLMNTSYDKFIRTTDDYHEKQVQKIFKKLYDQGDI
EEHHHCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHCCCCHHHHHHHHHHHHHHCCCCCE
YKGSYEGMYCTPCESFWTESQLVDGKCPDCGREVKPAKEEAYFFKMSKYANKLIEHINTH
ECCCCCCEEECCCHHHCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCC
PEFIQPVSRKNEMMNNFLLPGLQDLCVSRTSFKWGIPVDFDDKHVVYVWLDALTNYITGI
HHHHHHHHHHHHHHHHHCCCCHHHHHCCCCCCCCCCCCCCCCCEEEEEEHHHHHHHHHCC
GYDADGNSSEQYKKFWPADLHLIGKDIIRFHTIYWPIFLMALGEPLPKQVFGHPWLLQND
CCCCCCCCHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHCCCCCEEECC
GKMSKSKGNVIYADDLVDLFGVDAVRYFVLHEMPFENDGVISWELMVERMNSDLANTLGN
CCCCCCCCCEEEECHHHHHHHHHHHHHHHHHCCCCCCCCEEEHHHHHHHHHHHHHHHHHH
LVNRTIAMSNKYFGGVVNKTGVEDAAIDGDLKAVVTATRDKVQAKMATLHVADAITEVFT
HHHHHHHHCCCCCCCCCCCCCCCHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LFKRCNKYIDETMPWALAKDEAQQDRLAEVLYNLVESITIGANLLKSFMPETTDKILAQL
HHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHH
YPANPEAGVRDFDDLATFGLRETGLKVTETPEILFARLDFEKDLKEKVDAIQEAQKKANG
CCCCCCCCCCCHHHHHHHCHHHCCCEEECCHHHEEEECCCHHHHHHHHHHHHHHHHHHCC
VTEYPQVEVKPEITFDDFEKVQFRVAKVLTCEAVKKTKLLKFELQIGDEKRTIVSGIQKY
CCCCCCEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHEEEEEEEECCCHHHHHHHHHHH
YKPEELIGKKLVVCTNLKPRKICGIESQGMIISAWDDKDNLSVLTLEKDIIEGAEIG
CCCHHHHCCEEEEEECCCCHHCCCCCCCCEEEEECCCCCCCEEEEEEHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11792842 [H]