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The map label for this gene is pbpD [H]

Identifier: 238917842

GI number: 238917842

Start: 1991558

End: 1993780

Strand: Reverse

Name: pbpD [H]

Synonym: EUBELI_01923

Alternate gene names: 238917842

Gene position: 1993780-1991558 (Counterclockwise)

Preceding gene: 238917843

Following gene: 238917837

Centisome position: 92.99

GC content: 37.47

Gene sequence:

>2223_bases
ATGGCAAAAAAGAGATTATCAAAAAAGAAAAGAAGACGCAGATTAATAAGAAGAATTATACTGCTGGTGTTTTTGTTAGG
CATTGCTACAGGCCTGCTGTTGTTTTTACCGAAGATAATAAGAGTAATCAGTCTTGCACAGGATGCAAAGACAATGGTTA
ATGAAAGTTCTGTCAGTACATTTATGGAGTCGGGAACTACGGTTATATATGATGCAGACGGCAATGAACTCTGTACAATG
AGAGAATCTAAGGATATGTATTATGTGGGAATTGAAGATATCCCGGATACATTAGAACAGGCCTTTATAGTGATGGAAGA
TAAGGATTTCTATAAACATAAGGGAATTGATTTCAAAGCAATTATAAGGGCTGTTATAGCAAATACTGAAAATGACGAGA
TAGTGCAGGGAGCAAGTACTATTACACAGCAGCTGGCAAAGAATATTTTTCTTACACAGGAAGTTACATGGGAACGAAAG
GTTAAAGAAATATTTGTAGCGAGAGATATTGAAAAGAAATATTCCAAGGAGCAGATACTGGAATTTTACCTTAATAACAT
ATATTTTAGTAATGGGTATTATGGTGTAGGAGCTGCGGCAAAGGGATATTTTGGGAAAGAAGTAAAAGACCTTACTCTTT
CGGAACAGGCATTTATAGCGGCAATACCTAATAATCCTACAAGATATAATCCTCTGACTAACTTTGATAACACTGTAGAA
CGACGGGATAATATTCTGGCCAGACTGTATAATGAAGATATAATTAATTCAATGAATTATTATACAGCACTTGATTCACA
AGTTGAGCTGTCTAGGCAGCCTGTTTCGTCAAGGAATAATTCAGTTGAAACATATGCCAGACATTGTGCTACGGAAGGAA
TGATGCAGGCAGGCGGATTTATATTCAGAACAAATTTTATTAATGATGATGATTATGACCAGTATAAGAAGCTTTATGAA
GAAAGTTATACGATGTATCAGCAGAAATTATTATCAGGGGGATATAGTGTATATACAAGCATAGACATGAATATCCAGAA
AAAGCTGCAGTCAGCAGTTGATGATAATCTGGACAGTTATACTTCGATGAAGGATGGAATATATGAGATGCAGGCAGCAG
CAACATGTATTGATAATATGACAGGAAATGTGGTTGCTATTGTAGGTTCAAGAAGTCAGGAGCTGGAGGGATACACACTT
AACAGGGCATATCAAAGCTACAGACAGCCTGGAAGTTCAATTAAGCCAATATTAGACTATATTCCATATCTTCAAAAGGG
GAATACACCTGATACGATAGTATCAGATGAGTATATACAGGATGGTCCTAAAAATGCTGATGGAACTTATATGGGAAATA
TAACTTTAAGAACAGCGGTAAGCCTTTCAAGAAATACTGTTGCGTGGAATGTTCTTAAAGAGATAACTCCCAAGGTTGGC
AGTTCATATCTTCTTAATCAGGGATTCCATAAAATATGGATGGATAAGGATTATAATGCCATTGCTATCGGTGGTTTCAC
ATATGGAGTATCAACTGAAGAGATGGCGGGAGCTTATACAACGATAGTTAATGATGGAAAGTACCGGCGTGTAACATGTA
TAGTTTCAATATACGATAATAGAGGAAAGCTGGTGCTTGATACTTCTAACCGTGAGGAGAATATATATGATTCAGAGTCA
TGCAGGATGATGACAGATATGTTAAAAACTGTTGTGACAGAAGGAACCGGAAGAGGAGCAGCACCTGAAAATGCCATTGT
TGCGGGCAAGACAGGTACGACGAATTCTAATTATGATTCATGGTTTTGTGGATATAGTGCATATTATACACTGGCAGTAT
GGCAGGGATATGATTATCCGGCATCAATACCTCAGAATGCGACTTTGAAAATATTCAGACAGTTTATGGAACAGGCACAT
AATGGTCTTGCAAAGAAAGATTTTCCAAAGTATAGCCACAAAGAAAATCAAGAGGAAACACAGACAGAGACGCAGACAGA
AACTCAGTCAGTAACACAGACACAGACAGAAACACAATCAGTAACGCAGACGCAAAATAGTAGCAGTCATCATTATACAC
AGGCTTCAAGTCAGATGGAAACAGGAACGGTAAGGGATAATGATGCAACTGCAAGCACTAAGCAGGATAGTACAGCTGCT
GGAAATAATTCTGGTACAGACAGACCAGCGGAAACAAAGAATGATGCTGCAATAAGAAATTAA

Upstream 100 bases:

>100_bases
GAAAAATATTTCCATGAATGATGCCGCTAAAATGTTTTCACAGGGTGGAAATGTTTATGTAACATATTACAAATAATCAG
ATATTAAAAATTGGTGAAGA

Downstream 100 bases:

>100_bases
AAAATGGAATCTGGCTTAAATGTGTCAGATTCCATTTCTGTTATTAATTACTTTCCGTAATGAAGTTCGTTATATTCCTT
AACTCTCTGCTGGATTCTTT

Product: penicillin binding protein 1A

Products: NA

Alternate protein names: PBP 4 [H]

Number of amino acids: Translated: 740; Mature: 739

Protein sequence:

>740_residues
MAKKRLSKKKRRRRLIRRIILLVFLLGIATGLLLFLPKIIRVISLAQDAKTMVNESSVSTFMESGTTVIYDADGNELCTM
RESKDMYYVGIEDIPDTLEQAFIVMEDKDFYKHKGIDFKAIIRAVIANTENDEIVQGASTITQQLAKNIFLTQEVTWERK
VKEIFVARDIEKKYSKEQILEFYLNNIYFSNGYYGVGAAAKGYFGKEVKDLTLSEQAFIAAIPNNPTRYNPLTNFDNTVE
RRDNILARLYNEDIINSMNYYTALDSQVELSRQPVSSRNNSVETYARHCATEGMMQAGGFIFRTNFINDDDYDQYKKLYE
ESYTMYQQKLLSGGYSVYTSIDMNIQKKLQSAVDDNLDSYTSMKDGIYEMQAAATCIDNMTGNVVAIVGSRSQELEGYTL
NRAYQSYRQPGSSIKPILDYIPYLQKGNTPDTIVSDEYIQDGPKNADGTYMGNITLRTAVSLSRNTVAWNVLKEITPKVG
SSYLLNQGFHKIWMDKDYNAIAIGGFTYGVSTEEMAGAYTTIVNDGKYRRVTCIVSIYDNRGKLVLDTSNREENIYDSES
CRMMTDMLKTVVTEGTGRGAAPENAIVAGKTGTTNSNYDSWFCGYSAYYTLAVWQGYDYPASIPQNATLKIFRQFMEQAH
NGLAKKDFPKYSHKENQEETQTETQTETQSVTQTQTETQSVTQTQNSSSHHYTQASSQMETGTVRDNDATASTKQDSTAA
GNNSGTDRPAETKNDAAIRN

Sequences:

>Translated_740_residues
MAKKRLSKKKRRRRLIRRIILLVFLLGIATGLLLFLPKIIRVISLAQDAKTMVNESSVSTFMESGTTVIYDADGNELCTM
RESKDMYYVGIEDIPDTLEQAFIVMEDKDFYKHKGIDFKAIIRAVIANTENDEIVQGASTITQQLAKNIFLTQEVTWERK
VKEIFVARDIEKKYSKEQILEFYLNNIYFSNGYYGVGAAAKGYFGKEVKDLTLSEQAFIAAIPNNPTRYNPLTNFDNTVE
RRDNILARLYNEDIINSMNYYTALDSQVELSRQPVSSRNNSVETYARHCATEGMMQAGGFIFRTNFINDDDYDQYKKLYE
ESYTMYQQKLLSGGYSVYTSIDMNIQKKLQSAVDDNLDSYTSMKDGIYEMQAAATCIDNMTGNVVAIVGSRSQELEGYTL
NRAYQSYRQPGSSIKPILDYIPYLQKGNTPDTIVSDEYIQDGPKNADGTYMGNITLRTAVSLSRNTVAWNVLKEITPKVG
SSYLLNQGFHKIWMDKDYNAIAIGGFTYGVSTEEMAGAYTTIVNDGKYRRVTCIVSIYDNRGKLVLDTSNREENIYDSES
CRMMTDMLKTVVTEGTGRGAAPENAIVAGKTGTTNSNYDSWFCGYSAYYTLAVWQGYDYPASIPQNATLKIFRQFMEQAH
NGLAKKDFPKYSHKENQEETQTETQTETQSVTQTQTETQSVTQTQNSSSHHYTQASSQMETGTVRDNDATASTKQDSTAA
GNNSGTDRPAETKNDAAIRN
>Mature_739_residues
AKKRLSKKKRRRRLIRRIILLVFLLGIATGLLLFLPKIIRVISLAQDAKTMVNESSVSTFMESGTTVIYDADGNELCTMR
ESKDMYYVGIEDIPDTLEQAFIVMEDKDFYKHKGIDFKAIIRAVIANTENDEIVQGASTITQQLAKNIFLTQEVTWERKV
KEIFVARDIEKKYSKEQILEFYLNNIYFSNGYYGVGAAAKGYFGKEVKDLTLSEQAFIAAIPNNPTRYNPLTNFDNTVER
RDNILARLYNEDIINSMNYYTALDSQVELSRQPVSSRNNSVETYARHCATEGMMQAGGFIFRTNFINDDDYDQYKKLYEE
SYTMYQQKLLSGGYSVYTSIDMNIQKKLQSAVDDNLDSYTSMKDGIYEMQAAATCIDNMTGNVVAIVGSRSQELEGYTLN
RAYQSYRQPGSSIKPILDYIPYLQKGNTPDTIVSDEYIQDGPKNADGTYMGNITLRTAVSLSRNTVAWNVLKEITPKVGS
SYLLNQGFHKIWMDKDYNAIAIGGFTYGVSTEEMAGAYTTIVNDGKYRRVTCIVSIYDNRGKLVLDTSNREENIYDSESC
RMMTDMLKTVVTEGTGRGAAPENAIVAGKTGTTNSNYDSWFCGYSAYYTLAVWQGYDYPASIPQNATLKIFRQFMEQAHN
GLAKKDFPKYSHKENQEETQTETQTETQSVTQTQTETQSVTQTQNSSSHHYTQASSQMETGTVRDNDATASTKQDSTAAG
NNSGTDRPAETKNDAAIRN

Specific function: Cell Wall Formation. Synthesis Of Cross-Linked Peptidoglycan From The Lipid Intermediates. The Enzyme Has A Penicillin-Insensitive Transglycosylase N-Terminal Domain (Formation Of Linear Glycan Strands) And A Penicillin-Sensitive Transpeptidase C-Terminal

COG id: COG0744

COG function: function code M; Membrane carboxypeptidase (penicillin-binding protein)

Gene ontology:

Cell location: Cell membrane; Peripheral membrane protein (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the transpeptidase family [H]

Homologues:

Organism=Escherichia coli, GI1786343, Length=572, Percent_Identity=28.1468531468531, Blast_Score=188, Evalue=1e-48,
Organism=Escherichia coli, GI87082258, Length=298, Percent_Identity=36.9127516778523, Blast_Score=177, Evalue=1e-45,
Organism=Escherichia coli, GI1788867, Length=568, Percent_Identity=24.8239436619718, Blast_Score=134, Evalue=2e-32,
Organism=Escherichia coli, GI1789601, Length=252, Percent_Identity=32.9365079365079, Blast_Score=111, Evalue=2e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012338
- InterPro:   IPR001264
- InterPro:   IPR001460 [H]

Pfam domain/function: PF00912 Transgly; PF00905 Transpeptidase [H]

EC number: 2.4.1.129; 3.4.-.- [C]

Molecular weight: Translated: 83447; Mature: 83316

Theoretical pI: Translated: 5.30; Mature: 5.30

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKKRLSKKKRRRRLIRRIILLVFLLGIATGLLLFLPKIIRVISLAQDAKTMVNESSVST
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
FMESGTTVIYDADGNELCTMRESKDMYYVGIEDIPDTLEQAFIVMEDKDFYKHKGIDFKA
HHHCCCEEEEECCCCCEEEEECCCCEEEEECCHHHHHHHHEEEEEECCHHHHHCCCCHHH
IIRAVIANTENDEIVQGASTITQQLAKNIFLTQEVTWERKVKEIFVARDIEKKYSKEQIL
HHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
EFYLNNIYFSNGYYGVGAAAKGYFGKEVKDLTLSEQAFIAAIPNNPTRYNPLTNFDNTVE
HHHHHHEEECCCCEECCCCCCCCCCCHHHHCEECCCEEEEECCCCCCCCCCCCCCCHHHH
RRDNILARLYNEDIINSMNYYTALDSQVELSRQPVSSRNNSVETYARHCATEGMMQAGGF
HHHHHHHHHHHHHHHHCCHHHHHCCCHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHCCE
IFRTNFINDDDYDQYKKLYEESYTMYQQKLLSGGYSVYTSIDMNIQKKLQSAVDDNLDSY
EEEECCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECHHHHHHHHHHHHCCHHHH
TSMKDGIYEMQAAATCIDNMTGNVVAIVGSRSQELEGYTLNRAYQSYRQPGSSIKPILDY
HHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCHHHHHHHHHHCCCCCHHHHHHH
IPYLQKGNTPDTIVSDEYIQDGPKNADGTYMGNITLRTAVSLSRNTVAWNVLKEITPKVG
HHHHHCCCCCCCEECCHHHHCCCCCCCCEEEECEEEEEEHHHCCCHHHHHHHHHHHHHCC
SSYLLNQGFHKIWMDKDYNAIAIGGFTYGVSTEEMAGAYTTIVNDGKYRRVTCIVSIYDN
CHHHHHCCCHHEECCCCCCEEEECCEEECCCHHHHCCCEEEEECCCCEEEEEEEEEEECC
RGKLVLDTSNREENIYDSESCRMMTDMLKTVVTEGTGRGAAPENAIVAGKTGTTNSNYDS
CCEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCEEECCCCCCCCCCCC
WFCGYSAYYTLAVWQGYDYPASIPQNATLKIFRQFMEQAHNGLAKKDFPKYSHKENQEET
EECCHHHEEEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHH
QTETQTETQSVTQTQTETQSVTQTQNSSSHHYTQASSQMETGTVRDNDATASTKQDSTAA
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHCCCCCCCCCCCCCCCCCCCCCCCCC
GNNSGTDRPAETKNDAAIRN
CCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
AKKRLSKKKRRRRLIRRIILLVFLLGIATGLLLFLPKIIRVISLAQDAKTMVNESSVST
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
FMESGTTVIYDADGNELCTMRESKDMYYVGIEDIPDTLEQAFIVMEDKDFYKHKGIDFKA
HHHCCCEEEEECCCCCEEEEECCCCEEEEECCHHHHHHHHEEEEEECCHHHHHCCCCHHH
IIRAVIANTENDEIVQGASTITQQLAKNIFLTQEVTWERKVKEIFVARDIEKKYSKEQIL
HHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
EFYLNNIYFSNGYYGVGAAAKGYFGKEVKDLTLSEQAFIAAIPNNPTRYNPLTNFDNTVE
HHHHHHEEECCCCEECCCCCCCCCCCHHHHCEECCCEEEEECCCCCCCCCCCCCCCHHHH
RRDNILARLYNEDIINSMNYYTALDSQVELSRQPVSSRNNSVETYARHCATEGMMQAGGF
HHHHHHHHHHHHHHHHCCHHHHHCCCHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHCCE
IFRTNFINDDDYDQYKKLYEESYTMYQQKLLSGGYSVYTSIDMNIQKKLQSAVDDNLDSY
EEEECCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECHHHHHHHHHHHHCCHHHH
TSMKDGIYEMQAAATCIDNMTGNVVAIVGSRSQELEGYTLNRAYQSYRQPGSSIKPILDY
HHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCHHHHHHHHHHCCCCCHHHHHHH
IPYLQKGNTPDTIVSDEYIQDGPKNADGTYMGNITLRTAVSLSRNTVAWNVLKEITPKVG
HHHHHCCCCCCCEECCHHHHCCCCCCCCEEEECEEEEEEHHHCCCHHHHHHHHHHHHHCC
SSYLLNQGFHKIWMDKDYNAIAIGGFTYGVSTEEMAGAYTTIVNDGKYRRVTCIVSIYDN
CHHHHHCCCHHEECCCCCCEEEECCEEECCCHHHHCCCEEEEECCCCEEEEEEEEEEECC
RGKLVLDTSNREENIYDSESCRMMTDMLKTVVTEGTGRGAAPENAIVAGKTGTTNSNYDS
CCEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCEEECCCCCCCCCCCC
WFCGYSAYYTLAVWQGYDYPASIPQNATLKIFRQFMEQAHNGLAKKDFPKYSHKENQEET
EECCHHHEEEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHH
QTETQTETQSVTQTQTETQSVTQTQNSSSHHYTQASSQMETGTVRDNDATASTKQDSTAA
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHCCCCCCCCCCCCCCCCCCCCCCCCC
GNNSGTDRPAETKNDAAIRN
CCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Transferases; Acyltransferases; Aminoacyltransferases [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7961491; 9274030; 9384377 [H]