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The map label for this gene is yibK [C]

Identifier: 238917775

GI number: 238917775

Start: 1920354

End: 1920851

Strand: Reverse

Name: yibK [C]

Synonym: EUBELI_01856

Alternate gene names: 238917775

Gene position: 1920851-1920354 (Counterclockwise)

Preceding gene: 238917776

Following gene: 238917771

Centisome position: 89.58

GC content: 37.75

Gene sequence:

>498_bases
ATGTTGAATATAGTTTTACATGAACCAGAGATGCCGGCTAATACTGGCAATATAGGAAGAACATGTGTGGCAACAGGTGC
GAGGCTGCATCTTATAGGACCATTAGGATTTAAGATTAATGATAAGATGTTAAAGAGGGCAGGACTTGATTACTGGGATA
AGCTTGATGTTACTGAGTATGATGATTATAATGACTTTCTTGCTAAGAATCCGGGTGCTAAGGTATATATGGCTACAACC
AAGGCACATAAGACATATACAGAAGTTAATTATGAAGAAGACTGTTATATAATGTTTGGAAAGGAAAGTGCGGGGATACC
GGAGGAGATACTTGTTGATAATGAGGATACATGTATCAGAATTCCGATGATAGGAGATATAAGATCACTTAATCTTTCTA
ATTCAGTGGCAATCGTTCTTTACGAAGCACTCCGACAGCATGATTTTTCACACATGAATTGTGAGGGACATCTTCACAGA
CTTAACTGGAAGGAATAA

Upstream 100 bases:

>100_bases
AGTCATATGCCAGAAATGTATGTATACCGATGACATGTAACGATGATCTTATATTAGAATTAGATATTGCAGTAAAAGGT
GACTGATAAGGACAGAGGAT

Downstream 100 bases:

>100_bases
TAAAACACACGGACATACAAGTTATGCATGTCCGTGTGTTTTTTATTTTGCAAGTTCAAGTGTGAATATGAATTCTGTTC
CGACACCTAAAGTACTTACA

Product: TrmH family RNA methyltransferase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 165; Mature: 165

Protein sequence:

>165_residues
MLNIVLHEPEMPANTGNIGRTCVATGARLHLIGPLGFKINDKMLKRAGLDYWDKLDVTEYDDYNDFLAKNPGAKVYMATT
KAHKTYTEVNYEEDCYIMFGKESAGIPEEILVDNEDTCIRIPMIGDIRSLNLSNSVAIVLYEALRQHDFSHMNCEGHLHR
LNWKE

Sequences:

>Translated_165_residues
MLNIVLHEPEMPANTGNIGRTCVATGARLHLIGPLGFKINDKMLKRAGLDYWDKLDVTEYDDYNDFLAKNPGAKVYMATT
KAHKTYTEVNYEEDCYIMFGKESAGIPEEILVDNEDTCIRIPMIGDIRSLNLSNSVAIVLYEALRQHDFSHMNCEGHLHR
LNWKE
>Mature_165_residues
MLNIVLHEPEMPANTGNIGRTCVATGARLHLIGPLGFKINDKMLKRAGLDYWDKLDVTEYDDYNDFLAKNPGAKVYMATT
KAHKTYTEVNYEEDCYIMFGKESAGIPEEILVDNEDTCIRIPMIGDIRSLNLSNSVAIVLYEALRQHDFSHMNCEGHLHR
LNWKE

Specific function: Could methylate the ribose at the nucleotide 34 wobble position in tRNA [H]

COG id: COG0219

COG function: function code J; Predicted rRNA methylase (SpoU class)

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase TrmH family. TrmL subfamily [H]

Homologues:

Organism=Escherichia coli, GI1790034, Length=148, Percent_Identity=52.027027027027, Blast_Score=149, Evalue=7e-38,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004440
- InterPro:   IPR016914
- InterPro:   IPR001537 [H]

Pfam domain/function: PF00588 SpoU_methylase [H]

EC number: 2.1.1.- [C]

Molecular weight: Translated: 18807; Mature: 18807

Theoretical pI: Translated: 5.25; Mature: 5.25

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.4 %Cys     (Translated Protein)
4.2 %Met     (Translated Protein)
6.7 %Cys+Met (Translated Protein)
2.4 %Cys     (Mature Protein)
4.2 %Met     (Mature Protein)
6.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLNIVLHEPEMPANTGNIGRTCVATGARLHLIGPLGFKINDKMLKRAGLDYWDKLDVTEY
CEEEEEECCCCCCCCCCCCCEEEECCCEEEEEECCCCEECHHHHHHCCCCCCCCCCCCCC
DDYNDFLAKNPGAKVYMATTKAHKTYTEVNYEEDCYIMFGKESAGIPEEILVDNEDTCIR
CCCCHHHHCCCCCEEEEEECCCCCEEEECCCCCCEEEEECCCCCCCCHHEEECCCCCEEE
IPMIGDIRSLNLSNSVAIVLYEALRQHDFSHMNCEGHLHRLNWKE
EEECCCEEECCCCCCCHHHHHHHHHHCCCCCCCCCCEEEECCCCC
>Mature Secondary Structure
MLNIVLHEPEMPANTGNIGRTCVATGARLHLIGPLGFKINDKMLKRAGLDYWDKLDVTEY
CEEEEEECCCCCCCCCCCCCEEEECCCEEEEEECCCCEECHHHHHHCCCCCCCCCCCCCC
DDYNDFLAKNPGAKVYMATTKAHKTYTEVNYEEDCYIMFGKESAGIPEEILVDNEDTCIR
CCCCHHHHCCCCCEEEEEECCCCCEEEECCCCCCEEEEECCCCCCCCHHEEECCCCCEEE
IPMIGDIRSLNLSNSVAIVLYEALRQHDFSHMNCEGHLHRLNWKE
EEECCCEEECCCCCCCHHHHHHHHHHCCCCCCCCCCEEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11679669 [H]