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The map label for this gene is llaDCHIBM [H]

Identifier: 238917688

GI number: 238917688

Start: 1832529

End: 1833332

Strand: Reverse

Name: llaDCHIBM [H]

Synonym: EUBELI_01768

Alternate gene names: 238917688

Gene position: 1833332-1832529 (Counterclockwise)

Preceding gene: 238917689

Following gene: 238917687

Centisome position: 85.5

GC content: 39.3

Gene sequence:

>804_bases
ATGAATCAAGTTTGGGGTTCAAAGGTGCCATTATATTTCGAGACAGAACAATCACAGTTGGTATTAGGTGATTCCTTTAA
AATTCTAACAAAGATGAAACCGGAATCTGTTGATATGATATTTGCAGATCCACCTTATTTTTTAAGTAATGATGGCATTA
CCTGTCAGGGTGGGAAAATGGTTTCGGTAAATAAAGGCTCATGGGATAAGCTTTCGGAAAGTGGAACCGGCGTCGAAGAA
AAACACAAGTTTAACAGAAAGTGGATTAAGTTATGTAGGAAAGTACTAAAGCCGAACGGAACAATATGGATATCAGGAAC
ATTACACAATATATATTCGATTGGTATGGCATTGGAGCAGGAAGGCTTCAAAATCATCAACAACATTACATGGCAGAAAA
CAAATCCACCACCAAACTTAGCATGTCGATGCTTCACACATTCTACGGAAACCATATTATGGGCACAGAAGAATGATAAG
AAGTCTCGGCATTTTTTTGATTATCAGAAAATGAAAGAAATGAACGGTGGAAAGCAGATGAAGGATGTGTGGACAGGAGC
ATTGACGAAACCTTCGGAGAAAACAGAAGGTAAGCACCCTACGCAGAAACCGGAATATTTGCTTGAAAAAATTGTGCTGG
CATCGACGGAGGAAGGACAAGTTATCTTGGATCCTTTTTGTGGATCAGGAACTACCGGAGTAGAGGCGGTACGATTTGGA
CGAAAATTTATCGGAATAGATGTAAGTGAAGAATACTTGGAGATATCCAAGAGGAGATTGGAGAAGGTAGCAAATGACAA
ATAG

Upstream 100 bases:

>100_bases
TGTTGCATATGGATATATACCTAGAAATTGGAATGATGTAGGAGAATTGTATAATATCGTTTCAAGAATAAAACGTTAAT
TATAGAGAGGCGAAATATAT

Downstream 100 bases:

>100_bases
AAATTTTGATGAATGGCTGAGTAAGTTCAGACCGAGTATATCAAGTTATGATTATTATATCGACTTTGCAAAGGTCGTAA
GAAATGTAGATGAAATCAAG

Product: adenine-specific DNA-methyltransferase

Products: NA

Alternate protein names: M.LlaDCHI B; M.LlaDCHIB; Adenine-specific methyltransferase LlaDCHIB; M.LlaII B [H]

Number of amino acids: Translated: 267; Mature: 267

Protein sequence:

>267_residues
MNQVWGSKVPLYFETEQSQLVLGDSFKILTKMKPESVDMIFADPPYFLSNDGITCQGGKMVSVNKGSWDKLSESGTGVEE
KHKFNRKWIKLCRKVLKPNGTIWISGTLHNIYSIGMALEQEGFKIINNITWQKTNPPPNLACRCFTHSTETILWAQKNDK
KSRHFFDYQKMKEMNGGKQMKDVWTGALTKPSEKTEGKHPTQKPEYLLEKIVLASTEEGQVILDPFCGSGTTGVEAVRFG
RKFIGIDVSEEYLEISKRRLEKVANDK

Sequences:

>Translated_267_residues
MNQVWGSKVPLYFETEQSQLVLGDSFKILTKMKPESVDMIFADPPYFLSNDGITCQGGKMVSVNKGSWDKLSESGTGVEE
KHKFNRKWIKLCRKVLKPNGTIWISGTLHNIYSIGMALEQEGFKIINNITWQKTNPPPNLACRCFTHSTETILWAQKNDK
KSRHFFDYQKMKEMNGGKQMKDVWTGALTKPSEKTEGKHPTQKPEYLLEKIVLASTEEGQVILDPFCGSGTTGVEAVRFG
RKFIGIDVSEEYLEISKRRLEKVANDK
>Mature_267_residues
MNQVWGSKVPLYFETEQSQLVLGDSFKILTKMKPESVDMIFADPPYFLSNDGITCQGGKMVSVNKGSWDKLSESGTGVEE
KHKFNRKWIKLCRKVLKPNGTIWISGTLHNIYSIGMALEQEGFKIINNITWQKTNPPPNLACRCFTHSTETILWAQKNDK
KSRHFFDYQKMKEMNGGKQMKDVWTGALTKPSEKTEGKHPTQKPEYLLEKIVLASTEEGQVILDPFCGSGTTGVEAVRFG
RKFIGIDVSEEYLEISKRRLEKVANDK

Specific function: This methylase recognizes the double-stranded sequence GATC, causes specific methylation on A-2 on both strands, and protects the DNA from cleavage by the LlaDCHI endonuclease [H]

COG id: COG0863

COG function: function code L; DNA modification methylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the N(4)/N(6)-methyltransferase family [H]

Homologues:

Organism=Escherichia coli, GI87082238, Length=274, Percent_Identity=31.3868613138686, Blast_Score=114, Evalue=8e-27,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002941
- InterPro:   IPR002052
- InterPro:   IPR001091 [H]

Pfam domain/function: PF01555 N6_N4_Mtase [H]

EC number: =2.1.1.72 [H]

Molecular weight: Translated: 30348; Mature: 30348

Theoretical pI: Translated: 9.31; Mature: 9.31

Prosite motif: PS00092 N6_MTASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNQVWGSKVPLYFETEQSQLVLGDSFKILTKMKPESVDMIFADPPYFLSNDGITCQGGKM
CCCCCCCCCCEEEECCCCEEEECCCEEEEEECCCCCEEEEECCCCCEECCCCEEECCCEE
VSVNKGSWDKLSESGTGVEEKHKFNRKWIKLCRKVLKPNGTIWISGTLHNIYSIGMALEQ
EEECCCCCHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCCCEEEEEEHHHHHHHHHHHHHH
EGFKIINNITWQKTNPPPNLACRCFTHSTETILWAQKNDKKSRHFFDYQKMKEMNGGKQM
CCCEEECCCEECCCCCCCCCEEEEEECCCCEEEEEECCCCCCHHHHHHHHHHHHCCCCHH
KDVWTGALTKPSEKTEGKHPTQKPEYLLEKIVLASTEEGQVILDPFCGSGTTGVEAVRFG
HHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCEEEECCCCCCCCHHHHHHHC
RKFIGIDVSEEYLEISKRRLEKVANDK
CCEEECCCCHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MNQVWGSKVPLYFETEQSQLVLGDSFKILTKMKPESVDMIFADPPYFLSNDGITCQGGKM
CCCCCCCCCCEEEECCCCEEEECCCEEEEEECCCCCEEEEECCCCCEECCCCEEECCCEE
VSVNKGSWDKLSESGTGVEEKHKFNRKWIKLCRKVLKPNGTIWISGTLHNIYSIGMALEQ
EEECCCCCHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCCCEEEEEEHHHHHHHHHHHHHH
EGFKIINNITWQKTNPPPNLACRCFTHSTETILWAQKNDKKSRHFFDYQKMKEMNGGKQM
CCCEEECCCEECCCCCCCCCEEEEEECCCCEEEEEECCCCCCHHHHHHHHHHHHCCCCHH
KDVWTGALTKPSEKTEGKHPTQKPEYLLEKIVLASTEEGQVILDPFCGSGTTGVEAVRFG
HHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCEEEECCCCCCCCHHHHHHHC
RKFIGIDVSEEYLEISKRRLEKVANDK
CCEEECCCCHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7793939 [H]