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The map label for this gene is dpnB [H]

Identifier: 238917687

GI number: 238917687

Start: 1831676

End: 1832539

Strand: Reverse

Name: dpnB [H]

Synonym: EUBELI_01767

Alternate gene names: 238917687

Gene position: 1832539-1831676 (Counterclockwise)

Preceding gene: 238917688

Following gene: 238917686

Centisome position: 85.47

GC content: 35.53

Gene sequence:

>864_bases
ATGACAAATAGAAATTTTGATGAATGGCTGAGTAAGTTCAGACCGAGTATATCAAGTTATGATTATTATATCGACTTTGC
AAAGGTCGTAAGAAATGTAGATGAAATCAAGGTGGAATTAAACATCTTAAATTCCTTGATTGCTTCTAAAAATATTGAAG
AAGAATTTGAGAAGATAGTGACAAAGTATCCGGAAACTTTACAATGTGTTCCTCTGCTTCTTGCGGTGCGAGGTCATGAA
ATATATGCCCAGGATGAAGAAGGAGCTTTCCTATATAATTTCAAGAAAATGAATTACAGTGTGGAACAGTACAAGGTATT
TATGCGTAAGACCGGATTGTTTGATATGATTGCAAATCATTTGGTAAATAATCTTGTGGATTATGCTTTGGGTATCGAAA
CCGGATTGGACTCTAATGGTCGTAAGAATCGTGGCGGTCATCAGATGGAAGATTTGGTCGAGAAGTATATTGTGGCAGCA
GGTTTCAAAAAGAACGAGAATTACTTTAAAGAGATGTACTTAAAAGATATTGAATCTAAATGGAATATTGATTTGTCGGC
ACTTTCAAATCAGGGAAAAGCTGCAAAGAGATTTGATTTTGTAATCAAGACGGATAAGATGATTTATGGTATAGAAACAA
ACTTCTATGGCGGTGGCGGTTCAAAACTAAATGAAACTGCAAGAAGTTATAAAATGCTTGCGCAAGAGGCAGATACTATT
GAGGGCTTTACATTTGTGTGGTTTACGGATGGAATTGGCTGGAAGAGTGCAAGAGGTAATTTAAGAGAGACATTTGAGGT
TATGGATACGATTTACAGTATCAATGATATGGAGAATGGGGTTATGCAGAAATTATTTGAATAG

Upstream 100 bases:

>100_bases
ACCGGAGTAGAGGCGGTACGATTTGGACGAAAATTTATCGGAATAGATGTAAGTGAAGAATACTTGGAGATATCCAAGAG
GAGATTGGAGAAGGTAGCAA

Downstream 100 bases:

>100_bases
GAGCATATATGGTCGATAAAGATAAAGTTATTGAATTAATAGAGCGTGGACAAGAAACAGATTTTTGTGATTTTAAGAGA
GAATTTTACCATACAGCCAA

Product: type II restriction enzyme

Products: NA

Alternate protein names: R.DpnII; Endonuclease DpnII; Type II restriction enzyme DpnII [H]

Number of amino acids: Translated: 287; Mature: 286

Protein sequence:

>287_residues
MTNRNFDEWLSKFRPSISSYDYYIDFAKVVRNVDEIKVELNILNSLIASKNIEEEFEKIVTKYPETLQCVPLLLAVRGHE
IYAQDEEGAFLYNFKKMNYSVEQYKVFMRKTGLFDMIANHLVNNLVDYALGIETGLDSNGRKNRGGHQMEDLVEKYIVAA
GFKKNENYFKEMYLKDIESKWNIDLSALSNQGKAAKRFDFVIKTDKMIYGIETNFYGGGGSKLNETARSYKMLAQEADTI
EGFTFVWFTDGIGWKSARGNLRETFEVMDTIYSINDMENGVMQKLFE

Sequences:

>Translated_287_residues
MTNRNFDEWLSKFRPSISSYDYYIDFAKVVRNVDEIKVELNILNSLIASKNIEEEFEKIVTKYPETLQCVPLLLAVRGHE
IYAQDEEGAFLYNFKKMNYSVEQYKVFMRKTGLFDMIANHLVNNLVDYALGIETGLDSNGRKNRGGHQMEDLVEKYIVAA
GFKKNENYFKEMYLKDIESKWNIDLSALSNQGKAAKRFDFVIKTDKMIYGIETNFYGGGGSKLNETARSYKMLAQEADTI
EGFTFVWFTDGIGWKSARGNLRETFEVMDTIYSINDMENGVMQKLFE
>Mature_286_residues
TNRNFDEWLSKFRPSISSYDYYIDFAKVVRNVDEIKVELNILNSLIASKNIEEEFEKIVTKYPETLQCVPLLLAVRGHEI
YAQDEEGAFLYNFKKMNYSVEQYKVFMRKTGLFDMIANHLVNNLVDYALGIETGLDSNGRKNRGGHQMEDLVEKYIVAAG
FKKNENYFKEMYLKDIESKWNIDLSALSNQGKAAKRFDFVIKTDKMIYGIETNFYGGGGSKLNETARSYKMLAQEADTIE
GFTFVWFTDGIGWKSARGNLRETFEVMDTIYSINDMENGVMQKLFE

Specific function: Recognizes the double-stranded unmethylated sequence GATC and cleaves before G-1 [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the type II restriction enzyme DpnII family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR021191
- InterPro:   IPR007637 [H]

Pfam domain/function: PF04556 DpmII [H]

EC number: =3.1.21.4 [H]

Molecular weight: Translated: 33252; Mature: 33121

Theoretical pI: Translated: 5.01; Mature: 5.01

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTNRNFDEWLSKFRPSISSYDYYIDFAKVVRNVDEIKVELNILNSLIASKNIEEEFEKIV
CCCCCHHHHHHHHCCCCCCCHHHEEHHHHHCCHHHHHHHHHHHHHHHHCCCHHHHHHHHH
TKYPETLQCVPLLLAVRGHEIYAQDEEGAFLYNFKKMNYSVEQYKVFMRKTGLFDMIANH
HHCCHHHHHHHHHHHHCCCEEEEECCCCCEEEEHHHHCCCHHHHHHHHHHCCHHHHHHHH
LVNNLVDYALGIETGLDSNGRKNRGGHQMEDLVEKYIVAAGFKKNENYFKEMYLKDIESK
HHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHH
WNIDLSALSNQGKAAKRFDFVIKTDKMIYGIETNFYGGGGSKLNETARSYKMLAQEADTI
CCEEHHHHCCCCCHHHHHHEEEEECCEEEEEECCEECCCCCHHHHHHHHHHHHHHHHHHC
EGFTFVWFTDGIGWKSARGNLRETFEVMDTIYSINDMENGVMQKLFE
CCEEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
TNRNFDEWLSKFRPSISSYDYYIDFAKVVRNVDEIKVELNILNSLIASKNIEEEFEKIV
CCCCHHHHHHHHCCCCCCCHHHEEHHHHHCCHHHHHHHHHHHHHHHHCCCHHHHHHHHH
TKYPETLQCVPLLLAVRGHEIYAQDEEGAFLYNFKKMNYSVEQYKVFMRKTGLFDMIANH
HHCCHHHHHHHHHHHHCCCEEEEECCCCCEEEEHHHHCCCHHHHHHHHHHCCHHHHHHHH
LVNNLVDYALGIETGLDSNGRKNRGGHQMEDLVEKYIVAAGFKKNENYFKEMYLKDIESK
HHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHH
WNIDLSALSNQGKAAKRFDFVIKTDKMIYGIETNFYGGGGSKLNETARSYKMLAQEADTI
CCEEHHHHCCCCCHHHHHHEEEEECCEEEEEECCEECCCCCHHHHHHHHHHHHHHHHHHC
EGFTFVWFTDGIGWKSARGNLRETFEVMDTIYSINDMENGVMQKLFE
CCEEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 3019562; 2824782 [H]