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The map label for this gene is truA1 [H]

Identifier: 238917621

GI number: 238917621

Start: 1764070

End: 1764819

Strand: Reverse

Name: truA1 [H]

Synonym: EUBELI_01700

Alternate gene names: 238917621

Gene position: 1764819-1764070 (Counterclockwise)

Preceding gene: 238917622

Following gene: 238917618

Centisome position: 82.31

GC content: 41.33

Gene sequence:

>750_bases
ATGAAGAATTATAAGATGATAATTACTTATGATGGTACAAGGTATTATGGCTGGGAACATCAGCCAACTACAGATATGAC
AATTCAGGGCAAGTTAGAATCAGTGCTTTCTGCCATGACAGGAACTGAGGTTGAGGTTATAGGTGCAGGAAGAACAGATG
CCGGAGTGCATGCAAAGGGAATGGTAGCCAATGCGCATATGGAAACTAAGATGAAGGAAGATGATATATGCGATTATATG
AACAGATATCTTCCGGAAGATATCTGTGTGCAGGAGGTTCGTATTGCATCTGACAGATTCCACAGCCGTTACAATGCGCA
GGGGAAAACTTACTGCTATACATGTTATGTTGGGGATAAGAAGCCTGTATTTAACAGAAAATATGTCAATATAATTGAGG
GTAAGCTGGACGTTGAAGCCATGAAGAAGGCAGCAGACATACTTACTGGAGAACATGATTATGCATCATTTTGTGGTAAT
CCCAAGATGAAGAAATCGACAGTCCGTGAGATTTATTCGATAGATGTTTCTTTAAAGGGTTCATTCCTTAATCTTACATA
TCATGGAAGCGGATTCCTGCAGTACATGGTAAGAATATTAAGTGGTACACTTCTTGAAGTTGGACAGGGAAAGAGAACAC
CAGAGAGTATGTATGAACTTTTAGAGGAGAGAAACAGAAGCCTTGCAGGTGCAACTGCGCCGGCGAAGGGATTGTGTCTT
CTGAAGGTTGACTACTCGAAGGAGGCTTGA

Upstream 100 bases:

>100_bases
GAAAATGTGCTTATGGACAGTATTTTGCGAGATGAGATTACAGATGTTAATTGTATGAAATTATATGGCTTCAAATCAGA
AGAATGAATGGGAGATATGA

Downstream 100 bases:

>100_bases
TAGTCCGGACGCCGTACAGTGTGCTGTGGAAGGGTGTGAGATGTCCTTAGGCATCTGCCCTCAGGATTCTTATGTCTAGT
GAAACTAAATGCCGCAACCT

Product: tRNA-pseudouridine synthase I

Products: NA

Alternate protein names: tRNA pseudouridylate synthase I 1; tRNA-uridine isomerase I 1 [H]

Number of amino acids: Translated: 249; Mature: 249

Protein sequence:

>249_residues
MKNYKMIITYDGTRYYGWEHQPTTDMTIQGKLESVLSAMTGTEVEVIGAGRTDAGVHAKGMVANAHMETKMKEDDICDYM
NRYLPEDICVQEVRIASDRFHSRYNAQGKTYCYTCYVGDKKPVFNRKYVNIIEGKLDVEAMKKAADILTGEHDYASFCGN
PKMKKSTVREIYSIDVSLKGSFLNLTYHGSGFLQYMVRILSGTLLEVGQGKRTPESMYELLEERNRSLAGATAPAKGLCL
LKVDYSKEA

Sequences:

>Translated_249_residues
MKNYKMIITYDGTRYYGWEHQPTTDMTIQGKLESVLSAMTGTEVEVIGAGRTDAGVHAKGMVANAHMETKMKEDDICDYM
NRYLPEDICVQEVRIASDRFHSRYNAQGKTYCYTCYVGDKKPVFNRKYVNIIEGKLDVEAMKKAADILTGEHDYASFCGN
PKMKKSTVREIYSIDVSLKGSFLNLTYHGSGFLQYMVRILSGTLLEVGQGKRTPESMYELLEERNRSLAGATAPAKGLCL
LKVDYSKEA
>Mature_249_residues
MKNYKMIITYDGTRYYGWEHQPTTDMTIQGKLESVLSAMTGTEVEVIGAGRTDAGVHAKGMVANAHMETKMKEDDICDYM
NRYLPEDICVQEVRIASDRFHSRYNAQGKTYCYTCYVGDKKPVFNRKYVNIIEGKLDVEAMKKAADILTGEHDYASFCGN
PKMKKSTVREIYSIDVSLKGSFLNLTYHGSGFLQYMVRILSGTLLEVGQGKRTPESMYELLEERNRSLAGATAPAKGLCL
LKVDYSKEA

Specific function: Formation of pseudouridine at positions 38, 39 and 40 in the anticodon stem and loop of transfer RNAs [H]

COG id: COG0101

COG function: function code J; Pseudouridylate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tRNA pseudouridine synthase truA family [H]

Homologues:

Organism=Homo sapiens, GI23503259, Length=246, Percent_Identity=29.6747967479675, Blast_Score=89, Evalue=3e-18,
Organism=Escherichia coli, GI1788657, Length=241, Percent_Identity=37.344398340249, Blast_Score=155, Evalue=2e-39,
Organism=Caenorhabditis elegans, GI71985046, Length=284, Percent_Identity=25.7042253521127, Blast_Score=80, Evalue=8e-16,
Organism=Saccharomyces cerevisiae, GI6321112, Length=235, Percent_Identity=25.531914893617, Blast_Score=66, Evalue=4e-12,
Organism=Drosophila melanogaster, GI19922728, Length=261, Percent_Identity=26.8199233716475, Blast_Score=73, Evalue=2e-13,
Organism=Drosophila melanogaster, GI116007752, Length=302, Percent_Identity=25.1655629139073, Blast_Score=72, Evalue=3e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020103
- InterPro:   IPR001406
- InterPro:   IPR020097
- InterPro:   IPR020095
- InterPro:   IPR020094 [H]

Pfam domain/function: PF01416 PseudoU_synth_1 [H]

EC number: =5.4.99.12 [H]

Molecular weight: Translated: 28048; Mature: 28048

Theoretical pI: Translated: 7.82; Mature: 7.82

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.4 %Cys     (Translated Protein)
4.8 %Met     (Translated Protein)
7.2 %Cys+Met (Translated Protein)
2.4 %Cys     (Mature Protein)
4.8 %Met     (Mature Protein)
7.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKNYKMIITYDGTRYYGWEHQPTTDMTIQGKLESVLSAMTGTEVEVIGAGRTDAGVHAKG
CCCEEEEEEECCEEEECCCCCCCCCEEECHHHHHHHHHHCCCCEEEEECCCCCCCCCCCC
MVANAHMETKMKEDDICDYMNRYLPEDICVQEVRIASDRFHSRYNAQGKTYCYTCYVGDK
EEEECHHHHCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEEECCC
KPVFNRKYVNIIEGKLDVEAMKKAADILTGEHDYASFCGNPKMKKSTVREIYSIDVSLKG
CCCCCCHHEEEECCCCCHHHHHHHHHHHCCCCCHHHHCCCCCHHHHHHHHHHHCCEEECC
SFLNLTYHGSGFLQYMVRILSGTLLEVGQGKRTPESMYELLEERNRSLAGATAPAKGLCL
CEEEEEECCCHHHHHHHHHHCCHHEECCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCEEE
LKVDYSKEA
EEECCCCCC
>Mature Secondary Structure
MKNYKMIITYDGTRYYGWEHQPTTDMTIQGKLESVLSAMTGTEVEVIGAGRTDAGVHAKG
CCCEEEEEEECCEEEECCCCCCCCCEEECHHHHHHHHHHCCCCEEEEECCCCCCCCCCCC
MVANAHMETKMKEDDICDYMNRYLPEDICVQEVRIASDRFHSRYNAQGKTYCYTCYVGDK
EEEECHHHHCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEEECCC
KPVFNRKYVNIIEGKLDVEAMKKAADILTGEHDYASFCGNPKMKKSTVREIYSIDVSLKG
CCCCCCHHEEEECCCCCHHHHHHHHHHHCCCCCHHHHCCCCCHHHHHHHHHHHCCEEECC
SFLNLTYHGSGFLQYMVRILSGTLLEVGQGKRTPESMYELLEERNRSLAGATAPAKGLCL
CEEEEEECCCHHHHHHHHHHCCHHEECCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCEEE
LKVDYSKEA
EEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11792842 [H]