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The map label for this gene is pfkA [H]

Identifier: 238917601

GI number: 238917601

Start: 1739116

End: 1740090

Strand: Reverse

Name: pfkA [H]

Synonym: EUBELI_01680

Alternate gene names: 238917601

Gene position: 1740090-1739116 (Counterclockwise)

Preceding gene: 238917602

Following gene: 238917600

Centisome position: 81.15

GC content: 39.18

Gene sequence:

>975_bases
ATGGCAAAGGAAATTAAAACAATCGGCGTATTAACAAGTGGTGGAGATGCTCCAGGCATGAATGCTGCTATCCGTGCAGT
TGTAAGAACTGCTTTGAATAAGGGACTTAAGGTCAAGGGTATTCAGAAGGGCTATAACGGACTTCTTAATGATGAGATAA
TTGATATGGACAAGCGAAGCGTTGCTGATATTATCCAGCGTGGTGGAACAGTCCTCTATACAGCACGATGCATGGAATTC
ATGACTGAAGAAGGTCAGAAGAGAGGTGCTGAAGTATGCAGAAAGCATGGAATTGACGGTATAGTTGTAATCGGTGGAGA
TGGTTCTTTCAGAGGTGCACAGAAGCTTGCAGCACAGGGAATTAATACTATCGGACTTCCGGGAACAATTGATCTTGATA
TTGCATGTACAGATTATACTATTGGATTTGATACTGCTGTTAATACAGCAATGGAAGCTATTGATAAGATAAGAGATACA
TCTACATCACATGAAAGATGCAGTATTATCGAGGTTATGGGAAGAAATGCAGGATATATTGCATTGTGGTGTGGTATTGC
CAATGGTGCAGAGGATATCCTTCTTCCTGAAAGATATGATAATGATGAGCAGGCTCTTATTAATCATATTATAGAAGGAA
GAAAGAAGGGCAAGAAACACCATCTTATTATTAATGCTGAAGGTATCGGACATTCTACAGGAATGGCAAGAAGAATTGAA
GCTGCAACAGGTATTGAGACAAGAGCTACTATTTTAGGATACATGCAGCGTGGTGGTTCACCAACATGTAAGGACAGAAT
GTATGCGTCTATTATGGGTGCTTATGCTGTTGACCTTCTTGCTGAAGGAAAGAGTAACAGACTTGTAGCATACAGGGATG
GTAAGTTTGTTGATTATGATATTGATGAAGCACTTGCCATGACTAAGGATATTAATGATTATGAATTTAATATAAGTGCA
TTACTTTCTAAGTAA

Upstream 100 bases:

>100_bases
ATTAACATTGGTAAAAAATTATCAAAAAAAGAAGATTTTTCTTTTATAATGTGGTAACATAAAACAGAAAATAATATTAA
GAGATAAGAAAGGGGAAGTT

Downstream 100 bases:

>100_bases
TGCATGGCAGAAAGGTAACCTCATAATGATAACCAGTACGAGCAGTTCACAGGTAAAGCATGTTATATCATTGCTTTCTA
AGGCCAGGGAAAGAAAGAAG

Product: 6-phosphofructokinase

Products: NA

Alternate protein names: Phosphofructokinase; Phosphohexokinase [H]

Number of amino acids: Translated: 324; Mature: 323

Protein sequence:

>324_residues
MAKEIKTIGVLTSGGDAPGMNAAIRAVVRTALNKGLKVKGIQKGYNGLLNDEIIDMDKRSVADIIQRGGTVLYTARCMEF
MTEEGQKRGAEVCRKHGIDGIVVIGGDGSFRGAQKLAAQGINTIGLPGTIDLDIACTDYTIGFDTAVNTAMEAIDKIRDT
STSHERCSIIEVMGRNAGYIALWCGIANGAEDILLPERYDNDEQALINHIIEGRKKGKKHHLIINAEGIGHSTGMARRIE
AATGIETRATILGYMQRGGSPTCKDRMYASIMGAYAVDLLAEGKSNRLVAYRDGKFVDYDIDEALAMTKDINDYEFNISA
LLSK

Sequences:

>Translated_324_residues
MAKEIKTIGVLTSGGDAPGMNAAIRAVVRTALNKGLKVKGIQKGYNGLLNDEIIDMDKRSVADIIQRGGTVLYTARCMEF
MTEEGQKRGAEVCRKHGIDGIVVIGGDGSFRGAQKLAAQGINTIGLPGTIDLDIACTDYTIGFDTAVNTAMEAIDKIRDT
STSHERCSIIEVMGRNAGYIALWCGIANGAEDILLPERYDNDEQALINHIIEGRKKGKKHHLIINAEGIGHSTGMARRIE
AATGIETRATILGYMQRGGSPTCKDRMYASIMGAYAVDLLAEGKSNRLVAYRDGKFVDYDIDEALAMTKDINDYEFNISA
LLSK
>Mature_323_residues
AKEIKTIGVLTSGGDAPGMNAAIRAVVRTALNKGLKVKGIQKGYNGLLNDEIIDMDKRSVADIIQRGGTVLYTARCMEFM
TEEGQKRGAEVCRKHGIDGIVVIGGDGSFRGAQKLAAQGINTIGLPGTIDLDIACTDYTIGFDTAVNTAMEAIDKIRDTS
TSHERCSIIEVMGRNAGYIALWCGIANGAEDILLPERYDNDEQALINHIIEGRKKGKKHHLIINAEGIGHSTGMARRIEA
ATGIETRATILGYMQRGGSPTCKDRMYASIMGAYAVDLLAEGKSNRLVAYRDGKFVDYDIDEALAMTKDINDYEFNISAL
LSK

Specific function: Key control step of glycolysis. [C]

COG id: COG0205

COG function: function code G; 6-phosphofructokinase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the phosphofructokinase family [H]

Homologues:

Organism=Homo sapiens, GI48762920, Length=344, Percent_Identity=38.953488372093, Blast_Score=205, Evalue=4e-53,
Organism=Homo sapiens, GI11321601, Length=344, Percent_Identity=38.3720930232558, Blast_Score=203, Evalue=2e-52,
Organism=Homo sapiens, GI266453768, Length=344, Percent_Identity=37.5, Blast_Score=202, Evalue=2e-52,
Organism=Homo sapiens, GI266453748, Length=344, Percent_Identity=37.5, Blast_Score=202, Evalue=2e-52,
Organism=Homo sapiens, GI4505749, Length=344, Percent_Identity=37.5, Blast_Score=202, Evalue=2e-52,
Organism=Homo sapiens, GI266453619, Length=344, Percent_Identity=37.5, Blast_Score=202, Evalue=3e-52,
Organism=Escherichia coli, GI1790350, Length=305, Percent_Identity=52.4590163934426, Blast_Score=292, Evalue=2e-80,
Organism=Caenorhabditis elegans, GI17558788, Length=312, Percent_Identity=37.5, Blast_Score=185, Evalue=2e-47,
Organism=Caenorhabditis elegans, GI25147584, Length=311, Percent_Identity=37.9421221864952, Blast_Score=179, Evalue=2e-45,
Organism=Caenorhabditis elegans, GI72003601, Length=288, Percent_Identity=28.4722222222222, Blast_Score=114, Evalue=8e-26,
Organism=Saccharomyces cerevisiae, GI6321679, Length=352, Percent_Identity=35.2272727272727, Blast_Score=184, Evalue=2e-47,
Organism=Saccharomyces cerevisiae, GI6323861, Length=345, Percent_Identity=33.3333333333333, Blast_Score=181, Evalue=2e-46,
Organism=Drosophila melanogaster, GI24652337, Length=317, Percent_Identity=37.8548895899054, Blast_Score=184, Evalue=5e-47,
Organism=Drosophila melanogaster, GI17647809, Length=311, Percent_Identity=38.5852090032154, Blast_Score=184, Evalue=6e-47,
Organism=Drosophila melanogaster, GI28573326, Length=317, Percent_Identity=37.2239747634069, Blast_Score=181, Evalue=8e-46,

Paralogues:

None

Copy number: 3981 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 950 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012003
- InterPro:   IPR012828
- InterPro:   IPR022953
- InterPro:   IPR015912
- InterPro:   IPR000023 [H]

Pfam domain/function: PF00365 PFK [H]

EC number: =2.7.1.11 [H]

Molecular weight: Translated: 35027; Mature: 34895

Theoretical pI: Translated: 6.52; Mature: 6.52

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
5.6 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
5.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKEIKTIGVLTSGGDAPGMNAAIRAVVRTALNKGLKVKGIQKGYNGLLNDEIIDMDKRS
CCCCEEEEEEEECCCCCCCHHHHHHHHHHHHHHCCCEEEEHHHHCCCCCCCHHHCCCHHH
VADIIQRGGTVLYTARCMEFMTEEGQKRGAEVCRKHGIDGIVVIGGDGSFRGAQKLAAQG
HHHHHHCCCCEEEHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCHHHHHHHHCC
INTIGLPGTIDLDIACTDYTIGFDTAVNTAMEAIDKIRDTSTSHERCSIIEVMGRNAGYI
CCCCCCCCEEEEEEEEEEEEECHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCCCEE
ALWCGIANGAEDILLPERYDNDEQALINHIIEGRKKGKKHHLIINAEGIGHSTGMARRIE
EEEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCCHHHHHH
AATGIETRATILGYMQRGGSPTCKDRMYASIMGAYAVDLLAEGKSNRLVAYRDGKFVDYD
HHCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCEEEEC
IDEALAMTKDINDYEFNISALLSK
HHHHHHHHCCCCCCEEEHHEEECC
>Mature Secondary Structure 
AKEIKTIGVLTSGGDAPGMNAAIRAVVRTALNKGLKVKGIQKGYNGLLNDEIIDMDKRS
CCCEEEEEEEECCCCCCCHHHHHHHHHHHHHHCCCEEEEHHHHCCCCCCCHHHCCCHHH
VADIIQRGGTVLYTARCMEFMTEEGQKRGAEVCRKHGIDGIVVIGGDGSFRGAQKLAAQG
HHHHHHCCCCEEEHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCHHHHHHHHCC
INTIGLPGTIDLDIACTDYTIGFDTAVNTAMEAIDKIRDTSTSHERCSIIEVMGRNAGYI
CCCCCCCCEEEEEEEEEEEEECHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCCCEE
ALWCGIANGAEDILLPERYDNDEQALINHIIEGRKKGKKHHLIINAEGIGHSTGMARRIE
EEEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCCHHHHHH
AATGIETRATILGYMQRGGSPTCKDRMYASIMGAYAVDLLAEGKSNRLVAYRDGKFVDYD
HHCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCEEEEC
IDEALAMTKDINDYEFNISALLSK
HHHHHHHHCCCCCCEEEHHEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA