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The map label for this gene is dagK [H]

Identifier: 238917335

GI number: 238917335

Start: 1459502

End: 1460428

Strand: Reverse

Name: dagK [H]

Synonym: EUBELI_01412

Alternate gene names: 238917335

Gene position: 1460428-1459502 (Counterclockwise)

Preceding gene: 238917336

Following gene: 238917334

Centisome position: 68.11

GC content: 38.3

Gene sequence:

>927_bases
ATGGGACAGGTGATTATGATGAAGATGCTGTTTGTTTTTAATCCAAACTCAGGAAAAGCTCAGATTAAGAATCAGCTGAT
GAAGATAATACAGGTGTTTTCTAAGGCTGGATATGAGATTACTGTATATCCGACTAAGGCACCGCTTGATGGTTATCAGC
ACATTTTAGATAATGAAGGCAGGTATGATGTCATAACATGTAGTGGTGGAGATGGTACTCTTAATGAAACGGTTGCGGCT
GTTCTTAAGTATAAAGGAGAGAAGCCGCCGATAGGGTATATTCCTAGTGGAACAACGAATGATTTTGCTGCGAGTCTGGG
AATTCCAAGAAATATGACGAAGGCCGCTGTTAATATAGCAAAGGGTAAACGTTTTCCTTGTGATGTAGGAATTGTAAATG
GAGAACGCTCATTTAATTATGTTGCTGCATTTGGGGCGTTTACAAGGGTTTCTTATGGAACTCCACAGAATCTTAAGAAT
ATACTTGGACATCAGGCTTATGTAATTGAAGCAGTCAGAAGCTTATCAACTATTAAACCACAGTATATGCGTGTATATTC
TGAGGAGATGAATGTTGAGGGCAATTTTGTATATGGAATGATAAGCAATACAGATTCAGTTGGTGGAATAAAGAATCTTA
CCGGCAATGATGTTAATCTGCAGGATGGATTATTTGAAGTTACCATGATAAGAGAGCTTAACAATCCTATAGCAGTCCAA
TCGCTTATTAATGCTTTTATAACTAAAGACCTAAGCAAATGTGACTGGGTATGTACATGGAAGACCGAGTCTGTAAGGTT
TGAATCACCACAGCCTGTATCATGGACACTTGATGGTGAATATGGAGGAGAACATACAGAAATACAGTTTTCTGTTAAGG
AGAAAGCAGTTGATTTCCTGATAGGAGGAGTACCTGTTAATAACTAA

Upstream 100 bases:

>100_bases
CTGCTGGAAAGCGTCTCATGACAGTTATTACACACAATGAGGGTGGTACGAATAAAGATCTGGCTAAGTTTACAGATCAG
ATTAATGCATTATGTGATAA

Downstream 100 bases:

>100_bases
TTGATAATCATTAATAATATAAAGTGATAAAACCTTGTAAATTAATATGCAGGGTTTTGTCACTTTATTTTTTTACTAAT
TTTATACGAATAGGTAAAAA

Product: hypothetical protein

Products: NA

Alternate protein names: DAG kinase; DAGK [H]

Number of amino acids: Translated: 308; Mature: 307

Protein sequence:

>308_residues
MGQVIMMKMLFVFNPNSGKAQIKNQLMKIIQVFSKAGYEITVYPTKAPLDGYQHILDNEGRYDVITCSGGDGTLNETVAA
VLKYKGEKPPIGYIPSGTTNDFAASLGIPRNMTKAAVNIAKGKRFPCDVGIVNGERSFNYVAAFGAFTRVSYGTPQNLKN
ILGHQAYVIEAVRSLSTIKPQYMRVYSEEMNVEGNFVYGMISNTDSVGGIKNLTGNDVNLQDGLFEVTMIRELNNPIAVQ
SLINAFITKDLSKCDWVCTWKTESVRFESPQPVSWTLDGEYGGEHTEIQFSVKEKAVDFLIGGVPVNN

Sequences:

>Translated_308_residues
MGQVIMMKMLFVFNPNSGKAQIKNQLMKIIQVFSKAGYEITVYPTKAPLDGYQHILDNEGRYDVITCSGGDGTLNETVAA
VLKYKGEKPPIGYIPSGTTNDFAASLGIPRNMTKAAVNIAKGKRFPCDVGIVNGERSFNYVAAFGAFTRVSYGTPQNLKN
ILGHQAYVIEAVRSLSTIKPQYMRVYSEEMNVEGNFVYGMISNTDSVGGIKNLTGNDVNLQDGLFEVTMIRELNNPIAVQ
SLINAFITKDLSKCDWVCTWKTESVRFESPQPVSWTLDGEYGGEHTEIQFSVKEKAVDFLIGGVPVNN
>Mature_307_residues
GQVIMMKMLFVFNPNSGKAQIKNQLMKIIQVFSKAGYEITVYPTKAPLDGYQHILDNEGRYDVITCSGGDGTLNETVAAV
LKYKGEKPPIGYIPSGTTNDFAASLGIPRNMTKAAVNIAKGKRFPCDVGIVNGERSFNYVAAFGAFTRVSYGTPQNLKNI
LGHQAYVIEAVRSLSTIKPQYMRVYSEEMNVEGNFVYGMISNTDSVGGIKNLTGNDVNLQDGLFEVTMIRELNNPIAVQS
LINAFITKDLSKCDWVCTWKTESVRFESPQPVSWTLDGEYGGEHTEIQFSVKEKAVDFLIGGVPVNN

Specific function: Catalyzes the phosphorylation of diacylglycerol (DAG) into phosphatidic acid. Is a key enzyme involved in the production of lipoteichoic acid by reintroducing DAG formed from the breakdown of membrane phospholipids into the phosphatidylglycerol biosynthet

COG id: COG1597

COG function: function code IR; Sphingosine kinase and enzymes related to eukaryotic diacylglycerol kinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 DAGKc domain [H]

Homologues:

Organism=Escherichia coli, GI1788402, Length=276, Percent_Identity=27.8985507246377, Blast_Score=99, Evalue=3e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017438
- InterPro:   IPR005218
- InterPro:   IPR001206 [H]

Pfam domain/function: PF00781 DAGK_cat [H]

EC number: =2.7.1.107 [H]

Molecular weight: Translated: 33914; Mature: 33783

Theoretical pI: Translated: 6.77; Mature: 6.77

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGQVIMMKMLFVFNPNSGKAQIKNQLMKIIQVFSKAGYEITVYPTKAPLDGYQHILDNEG
CCHHHEEEEEEEECCCCCHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCHHHHHHHCCCC
RYDVITCSGGDGTLNETVAAVLKYKGEKPPIGYIPSGTTNDFAASLGIPRNMTKAAVNIA
CEEEEEEECCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHCCCCCCHHHHHHHHH
KGKRFPCDVGIVNGERSFNYVAAFGAFTRVSYGTPQNLKNILGHQAYVIEAVRSLSTIKP
CCCCCCCEEEEECCCCCCCEEEEHHHHHHCCCCCHHHHHHHHCCHHHHHHHHHHHHHCCH
QYMRVYSEEMNVEGNFVYGMISNTDSVGGIKNLTGNDVNLQDGLFEVTMIRELNNPIAVQ
HHHHHHHHHCCCCCCEEEEEECCCCCCCCCCCCCCCCCCCCCCCEEEEEHHHCCCCHHHH
SLINAFITKDLSKCDWVCTWKTESVRFESPQPVSWTLDGEYGGEHTEIQFSVKEKAVDFL
HHHHHHHHCCHHHCCEEEEECCCCEEECCCCCEEEEECCCCCCCCEEEEEEEHHHCCHHE
IGGVPVNN
ECCEECCC
>Mature Secondary Structure 
GQVIMMKMLFVFNPNSGKAQIKNQLMKIIQVFSKAGYEITVYPTKAPLDGYQHILDNEG
CHHHEEEEEEEECCCCCHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCHHHHHHHCCCC
RYDVITCSGGDGTLNETVAAVLKYKGEKPPIGYIPSGTTNDFAASLGIPRNMTKAAVNIA
CEEEEEEECCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHCCCCCCHHHHHHHHH
KGKRFPCDVGIVNGERSFNYVAAFGAFTRVSYGTPQNLKNILGHQAYVIEAVRSLSTIKP
CCCCCCCEEEEECCCCCCCEEEEHHHHHHCCCCCHHHHHHHHCCHHHHHHHHHHHHHCCH
QYMRVYSEEMNVEGNFVYGMISNTDSVGGIKNLTGNDVNLQDGLFEVTMIRELNNPIAVQ
HHHHHHHHHCCCCCCEEEEEECCCCCCCCCCCCCCCCCCCCCCCEEEEEHHHCCCCHHHH
SLINAFITKDLSKCDWVCTWKTESVRFESPQPVSWTLDGEYGGEHTEIQFSVKEKAVDFL
HHHHHHHHCCHHHCCEEEEECCCCEEECCCCCEEEEECCCCCCCCEEEEEEEHHHCCHHE
IGGVPVNN
ECCEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]