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The map label for this gene is eno

Identifier: 238917320

GI number: 238917320

Start: 1439909

End: 1441216

Strand: Reverse

Name: eno

Synonym: EUBELI_01395

Alternate gene names: 238917320

Gene position: 1441216-1439909 (Counterclockwise)

Preceding gene: 238917321

Following gene: 238917319

Centisome position: 67.21

GC content: 41.97

Gene sequence:

>1308_bases
ATGGCAGCATATTTAGAGATTGAAAAGGTTATAGGCAGAGAAATTCTTGATTCAAGAGGCAATCCTACAGTTGAGGCAGA
GGTTTATCTTGCGGATGGAACTGTTGGAAGAGGCGCGGCACCTTCAGGAGCATCTACAGGTGAATTTGAGGCGCTTGAGT
TAAGAGATAAGGATAAGTCAAGATATTTGGGAAAGGGCGTTACTAAGGCAGTAGAGAACATAAACACTGTTATTAATGAC
TGTCTCTTTGGAATAGATGCATCTGATATATATGCAGTTGATGCAGCGATGATTAAAGCTGATGGAACTAAGGATAAGTC
TAATCTTGGTGCAAATGCTATTCTTGCGGTATCTATTGCAGCAGCAAGAGCAGCATCTGTATCTTTAGATATTCCTCTTT
ACAGGTTCTTAGGCGGAGTATCAGGAAACAGGCTTCCTGTTCCAATGATGAATATTATTAATGGAGGCTGCCATGCATTA
TCAAGCGGACTTGATGTTCAGGAATTCATGATTATGCCTGTTGGTGCACCAAGCTTTAAGGAATGCTTAAGATGGTGTGC
AGAGGTATTTCATGCACTTGCAGCTATACTTAAAGAAAGAGGACTTGCAACCTCTGTAGGTGATGAGGGCGGTTTTGCTC
CGGCACTTAAGTCGGATGAGGAAGCAATTGAGACAATATTACAGGCAGTAGAAAAGGCTGGATATAAGCCGGGCAGGGAC
TTTAGGATTGCAATGGATGCAGCTTCTTCAGAGTGGAAGAGTGAGAAAGGAAAGGGTTATTACAAGCTTCCTAAGGCTGG
AACAGAGTATACAGCAGAGGAGCTTATTGAGCACTGGGCTAAATTATGTGAGAAGTATCCTATTATATCTATTGAAGATG
GTCTTGATGAAGAGGACTGGGAAGGCTGGAAGAAGCTTACAGACAGACTTGGAGATAAGGTTCAGCTTGTAGGTGATGAT
CTTTTTGTTACTAATACAGAGAGACTCTCTAAGGGAATTGAACTTGGAGCAGGTAATGCAATTCTTATCAAGCTTAATCA
GATTGGTTCAGTTTCAGAGACACTTGAAGCTATCAAGATGGCTCATAAGGCAGGATATACAGCAATCAGCTCACACAGAT
CAGGTGAGACAGCAGATACAACAATTGCAGACCTCGCTGTTGCTCTTAACACTTGCCAGATTAAGACAGGTGCTCCTTCA
AGATCAGAAAGAGTTGCTAAGTATAACCAGCTTTTAAGAATTGAGGAACAGCTTGGCGCCAGTGCGGTATATCCGGGAAT
CAGGGCTTTTAATGTTAATAATGATTAA

Upstream 100 bases:

>100_bases
TCTTGCATTGTGGATTTGGTTGTATAATGCTAGAATTGTATTGCTAAAGTTAGAAGAAACTAACTTAAAAGATTACAAAG
CATTTGATTAGGAGGATTAT

Downstream 100 bases:

>100_bases
TCATTTGAAACAGATATTGAAAAAATATTAAGAAAGCCTTATAGTTGTGTATGATTATAATATATGTAACAGGAAGGCGG
AACAATGATTAAGAAATTAG

Product: enolase

Products: NA

Alternate protein names: 2-phospho-D-glycerate hydro-lyase; 2-phosphoglycerate dehydratase

Number of amino acids: Translated: 435; Mature: 434

Protein sequence:

>435_residues
MAAYLEIEKVIGREILDSRGNPTVEAEVYLADGTVGRGAAPSGASTGEFEALELRDKDKSRYLGKGVTKAVENINTVIND
CLFGIDASDIYAVDAAMIKADGTKDKSNLGANAILAVSIAAARAASVSLDIPLYRFLGGVSGNRLPVPMMNIINGGCHAL
SSGLDVQEFMIMPVGAPSFKECLRWCAEVFHALAAILKERGLATSVGDEGGFAPALKSDEEAIETILQAVEKAGYKPGRD
FRIAMDAASSEWKSEKGKGYYKLPKAGTEYTAEELIEHWAKLCEKYPIISIEDGLDEEDWEGWKKLTDRLGDKVQLVGDD
LFVTNTERLSKGIELGAGNAILIKLNQIGSVSETLEAIKMAHKAGYTAISSHRSGETADTTIADLAVALNTCQIKTGAPS
RSERVAKYNQLLRIEEQLGASAVYPGIRAFNVNND

Sequences:

>Translated_435_residues
MAAYLEIEKVIGREILDSRGNPTVEAEVYLADGTVGRGAAPSGASTGEFEALELRDKDKSRYLGKGVTKAVENINTVIND
CLFGIDASDIYAVDAAMIKADGTKDKSNLGANAILAVSIAAARAASVSLDIPLYRFLGGVSGNRLPVPMMNIINGGCHAL
SSGLDVQEFMIMPVGAPSFKECLRWCAEVFHALAAILKERGLATSVGDEGGFAPALKSDEEAIETILQAVEKAGYKPGRD
FRIAMDAASSEWKSEKGKGYYKLPKAGTEYTAEELIEHWAKLCEKYPIISIEDGLDEEDWEGWKKLTDRLGDKVQLVGDD
LFVTNTERLSKGIELGAGNAILIKLNQIGSVSETLEAIKMAHKAGYTAISSHRSGETADTTIADLAVALNTCQIKTGAPS
RSERVAKYNQLLRIEEQLGASAVYPGIRAFNVNND
>Mature_434_residues
AAYLEIEKVIGREILDSRGNPTVEAEVYLADGTVGRGAAPSGASTGEFEALELRDKDKSRYLGKGVTKAVENINTVINDC
LFGIDASDIYAVDAAMIKADGTKDKSNLGANAILAVSIAAARAASVSLDIPLYRFLGGVSGNRLPVPMMNIINGGCHALS
SGLDVQEFMIMPVGAPSFKECLRWCAEVFHALAAILKERGLATSVGDEGGFAPALKSDEEAIETILQAVEKAGYKPGRDF
RIAMDAASSEWKSEKGKGYYKLPKAGTEYTAEELIEHWAKLCEKYPIISIEDGLDEEDWEGWKKLTDRLGDKVQLVGDDL
FVTNTERLSKGIELGAGNAILIKLNQIGSVSETLEAIKMAHKAGYTAISSHRSGETADTTIADLAVALNTCQIKTGAPSR
SERVAKYNQLLRIEEQLGASAVYPGIRAFNVNND

Specific function: Catalyzes the reversible conversion of 2- phosphoglycerate into phosphoenolpyruvate. It is essential for the degradation of carbohydrates via glycolysis

COG id: COG0148

COG function: function code G; Enolase

Gene ontology:

Cell location: Cytoplasm. Secreted. Cell surface. Note=Fractions of enolase are present in both the cytoplasm and on the cell surface. The export of enolase possibly depends on the covalent binding to the substrate; once secreted, it remains attached to the cell surface

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the enolase family

Homologues:

Organism=Homo sapiens, GI5803011, Length=431, Percent_Identity=50.1160092807425, Blast_Score=400, Evalue=1e-111,
Organism=Homo sapiens, GI4503571, Length=431, Percent_Identity=49.6519721577726, Blast_Score=397, Evalue=1e-110,
Organism=Homo sapiens, GI301897477, Length=436, Percent_Identity=50, Blast_Score=394, Evalue=1e-110,
Organism=Homo sapiens, GI301897469, Length=436, Percent_Identity=50, Blast_Score=394, Evalue=1e-110,
Organism=Homo sapiens, GI301897479, Length=434, Percent_Identity=45.6221198156682, Blast_Score=345, Evalue=5e-95,
Organism=Homo sapiens, GI169201331, Length=337, Percent_Identity=27.0029673590504, Blast_Score=99, Evalue=6e-21,
Organism=Homo sapiens, GI169201757, Length=337, Percent_Identity=27.0029673590504, Blast_Score=99, Evalue=6e-21,
Organism=Homo sapiens, GI239744207, Length=337, Percent_Identity=27.0029673590504, Blast_Score=99, Evalue=6e-21,
Organism=Escherichia coli, GI1789141, Length=426, Percent_Identity=59.3896713615024, Blast_Score=472, Evalue=1e-134,
Organism=Caenorhabditis elegans, GI71995829, Length=432, Percent_Identity=50.462962962963, Blast_Score=389, Evalue=1e-108,
Organism=Caenorhabditis elegans, GI17536383, Length=432, Percent_Identity=50.462962962963, Blast_Score=388, Evalue=1e-108,
Organism=Caenorhabditis elegans, GI32563855, Length=187, Percent_Identity=50.8021390374332, Blast_Score=180, Evalue=1e-45,
Organism=Saccharomyces cerevisiae, GI6321693, Length=434, Percent_Identity=48.6175115207373, Blast_Score=363, Evalue=1e-101,
Organism=Saccharomyces cerevisiae, GI6323985, Length=441, Percent_Identity=45.578231292517, Blast_Score=349, Evalue=5e-97,
Organism=Saccharomyces cerevisiae, GI6324974, Length=441, Percent_Identity=45.3514739229025, Blast_Score=347, Evalue=2e-96,
Organism=Saccharomyces cerevisiae, GI6324969, Length=441, Percent_Identity=45.3514739229025, Blast_Score=347, Evalue=2e-96,
Organism=Saccharomyces cerevisiae, GI6321968, Length=434, Percent_Identity=48.3870967741936, Blast_Score=343, Evalue=4e-95,
Organism=Drosophila melanogaster, GI24580918, Length=439, Percent_Identity=46.9248291571754, Blast_Score=356, Evalue=2e-98,
Organism=Drosophila melanogaster, GI24580916, Length=439, Percent_Identity=46.9248291571754, Blast_Score=356, Evalue=2e-98,
Organism=Drosophila melanogaster, GI24580920, Length=439, Percent_Identity=46.9248291571754, Blast_Score=356, Evalue=2e-98,
Organism=Drosophila melanogaster, GI24580914, Length=439, Percent_Identity=46.9248291571754, Blast_Score=356, Evalue=2e-98,
Organism=Drosophila melanogaster, GI281360527, Length=439, Percent_Identity=46.9248291571754, Blast_Score=356, Evalue=2e-98,
Organism=Drosophila melanogaster, GI17137654, Length=439, Percent_Identity=46.9248291571754, Blast_Score=356, Evalue=2e-98,

Paralogues:

None

Copy number: 200 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1660 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 20 Molecules/Cell In: Stationary Phase,

Swissprot (AC and ID): ENO_EUBE2 (C4Z1M9)

Other databases:

- EMBL:   CP001104
- RefSeq:   YP_002930837.1
- ProteinModelPortal:   C4Z1M9
- GeneID:   7958250
- GenomeReviews:   CP001104_GR
- KEGG:   eel:EUBELI_01395
- OMA:   DIAVGTN
- ProtClustDB:   CLSK2548349
- GO:   GO:0006096
- HAMAP:   MF_00318
- InterPro:   IPR000941
- InterPro:   IPR020810
- InterPro:   IPR020809
- InterPro:   IPR020811
- PIRSF:   PIRSF001400
- PRINTS:   PR00148
- TIGRFAMs:   TIGR01060

Pfam domain/function: PF00113 Enolase_C; PF03952 Enolase_N

EC number: =4.2.1.11

Molecular weight: Translated: 46671; Mature: 46540

Theoretical pI: Translated: 4.75; Mature: 4.75

Prosite motif: PS00164 ENOLASE

Important sites: ACT_SITE 209-209 ACT_SITE 344-344 BINDING 158-158 BINDING 168-168 BINDING 292-292 BINDING 319-319 BINDING 344-344 BINDING 395-395

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAAYLEIEKVIGREILDSRGNPTVEAEVYLADGTVGRGAAPSGASTGEFEALELRDKDKS
CCCHHHHHHHHHHHHHHCCCCCCEEEEEEEECCCCCCCCCCCCCCCCCEEEEEECCCHHH
RYLGKGVTKAVENINTVINDCLFGIDASDIYAVDAAMIKADGTKDKSNLGANAILAVSIA
HHHHHHHHHHHHHHHHHHHHHHHCCCCCHHEEHHHHEEECCCCCCCCCCCCCHHHHHHHH
AARAASVSLDIPLYRFLGGVSGNRLPVPMMNIINGGCHALSSGLDVQEFMIMPVGAPSFK
HHHHCEEEECCHHHHHHCCCCCCCCCCHHHHHHCCHHHHHHCCCCHHHEEEECCCCCCHH
ECLRWCAEVFHALAAILKERGLATSVGDEGGFAPALKSDEEAIETILQAVEKAGYKPGRD
HHHHHHHHHHHHHHHHHHHCCCEECCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCC
FRIAMDAASSEWKSEKGKGYYKLPKAGTEYTAEELIEHWAKLCEKYPIISIEDGLDEEDW
EEEEEECCCHHHHHHCCCCEEECCCCCCCHHHHHHHHHHHHHHHHCCEEEECCCCCHHHH
EGWKKLTDRLGDKVQLVGDDLFVTNTERLSKGIELGAGNAILIKLNQIGSVSETLEAIKM
HHHHHHHHHCCCEEEEECCCEEEECHHHHHCCCEECCCCEEEEEECCCCCHHHHHHHHHH
AHKAGYTAISSHRSGETADTTIADLAVALNTCQIKTGAPSRSERVAKYNQLLRIEEQLGA
HHHCCCHHHHCCCCCCCHHHHHHHHHHHHHHEEEECCCCCHHHHHHHHHHHHHHHHHHCC
SAVYPGIRAFNVNND
HHHCCCCEEECCCCC
>Mature Secondary Structure 
AAYLEIEKVIGREILDSRGNPTVEAEVYLADGTVGRGAAPSGASTGEFEALELRDKDKS
CCHHHHHHHHHHHHHHCCCCCCEEEEEEEECCCCCCCCCCCCCCCCCEEEEEECCCHHH
RYLGKGVTKAVENINTVINDCLFGIDASDIYAVDAAMIKADGTKDKSNLGANAILAVSIA
HHHHHHHHHHHHHHHHHHHHHHHCCCCCHHEEHHHHEEECCCCCCCCCCCCCHHHHHHHH
AARAASVSLDIPLYRFLGGVSGNRLPVPMMNIINGGCHALSSGLDVQEFMIMPVGAPSFK
HHHHCEEEECCHHHHHHCCCCCCCCCCHHHHHHCCHHHHHHCCCCHHHEEEECCCCCCHH
ECLRWCAEVFHALAAILKERGLATSVGDEGGFAPALKSDEEAIETILQAVEKAGYKPGRD
HHHHHHHHHHHHHHHHHHHCCCEECCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCC
FRIAMDAASSEWKSEKGKGYYKLPKAGTEYTAEELIEHWAKLCEKYPIISIEDGLDEEDW
EEEEEECCCHHHHHHCCCCEEECCCCCCCHHHHHHHHHHHHHHHHCCEEEECCCCCHHHH
EGWKKLTDRLGDKVQLVGDDLFVTNTERLSKGIELGAGNAILIKLNQIGSVSETLEAIKM
HHHHHHHHHCCCEEEEECCCEEEECHHHHHCCCEECCCCEEEEEECCCCCHHHHHHHHHH
AHKAGYTAISSHRSGETADTTIADLAVALNTCQIKTGAPSRSERVAKYNQLLRIEEQLGA
HHHCCCHHHHCCCCCCCHHHHHHHHHHHHHHEEEECCCCCHHHHHHHHHHHHHHHHHHCC
SAVYPGIRAFNVNND
HHHCCCCEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA