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The map label for this gene is glyQS

Identifier: 238917275

GI number: 238917275

Start: 1387645

End: 1389033

Strand: Reverse

Name: glyQS

Synonym: EUBELI_01350

Alternate gene names: 238917275

Gene position: 1389033-1387645 (Counterclockwise)

Preceding gene: 238917278

Following gene: 238917274

Centisome position: 64.78

GC content: 40.1

Gene sequence:

>1389_bases
ATGGAAAAGACAATGGAGAAAATTGTTTCTTTAGCTAAGGCGAGAGGATTTGTTTACCCAGGTTCTGAAATCTATGGAGG
ATTAGCTAATACATGGGATTATGGTAATCTTGGTGTTGAGTTAAAGAACAATGTTAAGAAGGCATGGTGGCAGAAGTTCG
TACAGGAAAGCCCATATAATGTTGGTGTTGATTGTGCAATTCTTATGAACCCTCAGACTTGGGTAGCATCAGGTCATCTT
GGTGGATTTTCTGATCCATTAATGGACTGCAAGGAATGTCATGAGAGATTCAGAGCAGATAAACTTATAGAAGACTGGGC
AGATGAGAATTCATATGATCTTGGCGGTTCAGTTGATGGATGGACACAGGAGCAGATGAAGAATTTCATTGATGAGAAGA
ATATCTGCTGTCCTTCATGCGGTAAGCACAATTTCACAGATATCAGACAGTTCAACCTTATGTTCAAGACATTCCAGGGT
GTAACTGAGGATGCCAAGAATACAGTATATTTAAGACCGGAGACAGCTCAGGGTATCTTCGTTAACTTCAAGAATGTCCA
GAGAACATCAAGAAAGAAAGTGCCTTTTGGTATCGGACAGATTGGTAAGTCATTCCGTAACGAGATTACACCAGGTAACT
TCACATTCAGAACAAGAGAGTTTGAGCAGATGGAATTAGAGTTCTTCTGCAAGCCTGGAACAGATCTTGAGTGGTTCACA
TACTGGAGACAGTACTGCATAGACTGGCTTAAGGCATTAGGAATTAAGGAAGATGAAATGAGAGCAAGAGATCATTCACC
AGAAGAACTCTGCTTCTACTCTAAGGGAACAACAGATATCGAATTCCTCTTCCCATTCGGATGGGGCGAACTCTGGGGTA
TTGCTGACAGAACAGACTATGACCTTACACAGCATCAGACAGTATCTGGCGAAGATATGTCATACTTTGATGATGAAGCT
AAGGAGAAGTATATACCATATGTTATCGAGCCTTCACTTGGCGCAGACCGTGTAACATTAGCATTCCTCTGCTCAGCTTA
TGACGAAGAAGAACTTGAAGGAGGAGATGTAAGAACAGTTCTTCATTTCCACCCTGCAATAGCACCAGTTAAGATTGGTA
TTCTTCCACTTTCTAAGAAGTTAAATGAAGGCGCAGAAAAAGTATATGCAGAGCTTTCTAAGTACTATAACTGTGAATTT
GATGACAGAGGTAATATCGGAAAGAGATACAGAAGACAGGATGAGATTGGTACACCATTCTGTATCACATATGATTTTGA
TTCGGAAGAAGATGGTGCTGTAACTGTAAGAGATAGGGATACAATGCAGCAGGAAAGAATAAAGATAGCAGATCTTAAGG
CATATTTTGAAGATAAGTTTAGATTTTAA

Upstream 100 bases:

>100_bases
TTGCTAATCAGCACTAAATGTTATAAAATAATTAAGATTATGTAGCAGCCGATGCAGTTCGATGTAGTCAGGCTGTTGCC
TTTAGGAGGATTATATTATT

Downstream 100 bases:

>100_bases
TTAATAAAAGAACAGAATTTAAATCGATAATTAAAAGGAAAGAATTATGCTGTGTACTAAATGTGGTCAGGAATATGAGG
GCAGTCAGTGCCCAAGATGT

Product: glycyl-tRNA synthetase

Products: NA

Alternate protein names: Glycine--tRNA ligase; GlyRS

Number of amino acids: Translated: 462; Mature: 462

Protein sequence:

>462_residues
MEKTMEKIVSLAKARGFVYPGSEIYGGLANTWDYGNLGVELKNNVKKAWWQKFVQESPYNVGVDCAILMNPQTWVASGHL
GGFSDPLMDCKECHERFRADKLIEDWADENSYDLGGSVDGWTQEQMKNFIDEKNICCPSCGKHNFTDIRQFNLMFKTFQG
VTEDAKNTVYLRPETAQGIFVNFKNVQRTSRKKVPFGIGQIGKSFRNEITPGNFTFRTREFEQMELEFFCKPGTDLEWFT
YWRQYCIDWLKALGIKEDEMRARDHSPEELCFYSKGTTDIEFLFPFGWGELWGIADRTDYDLTQHQTVSGEDMSYFDDEA
KEKYIPYVIEPSLGADRVTLAFLCSAYDEEELEGGDVRTVLHFHPAIAPVKIGILPLSKKLNEGAEKVYAELSKYYNCEF
DDRGNIGKRYRRQDEIGTPFCITYDFDSEEDGAVTVRDRDTMQQERIKIADLKAYFEDKFRF

Sequences:

>Translated_462_residues
MEKTMEKIVSLAKARGFVYPGSEIYGGLANTWDYGNLGVELKNNVKKAWWQKFVQESPYNVGVDCAILMNPQTWVASGHL
GGFSDPLMDCKECHERFRADKLIEDWADENSYDLGGSVDGWTQEQMKNFIDEKNICCPSCGKHNFTDIRQFNLMFKTFQG
VTEDAKNTVYLRPETAQGIFVNFKNVQRTSRKKVPFGIGQIGKSFRNEITPGNFTFRTREFEQMELEFFCKPGTDLEWFT
YWRQYCIDWLKALGIKEDEMRARDHSPEELCFYSKGTTDIEFLFPFGWGELWGIADRTDYDLTQHQTVSGEDMSYFDDEA
KEKYIPYVIEPSLGADRVTLAFLCSAYDEEELEGGDVRTVLHFHPAIAPVKIGILPLSKKLNEGAEKVYAELSKYYNCEF
DDRGNIGKRYRRQDEIGTPFCITYDFDSEEDGAVTVRDRDTMQQERIKIADLKAYFEDKFRF
>Mature_462_residues
MEKTMEKIVSLAKARGFVYPGSEIYGGLANTWDYGNLGVELKNNVKKAWWQKFVQESPYNVGVDCAILMNPQTWVASGHL
GGFSDPLMDCKECHERFRADKLIEDWADENSYDLGGSVDGWTQEQMKNFIDEKNICCPSCGKHNFTDIRQFNLMFKTFQG
VTEDAKNTVYLRPETAQGIFVNFKNVQRTSRKKVPFGIGQIGKSFRNEITPGNFTFRTREFEQMELEFFCKPGTDLEWFT
YWRQYCIDWLKALGIKEDEMRARDHSPEELCFYSKGTTDIEFLFPFGWGELWGIADRTDYDLTQHQTVSGEDMSYFDDEA
KEKYIPYVIEPSLGADRVTLAFLCSAYDEEELEGGDVRTVLHFHPAIAPVKIGILPLSKKLNEGAEKVYAELSKYYNCEF
DDRGNIGKRYRRQDEIGTPFCITYDFDSEEDGAVTVRDRDTMQQERIKIADLKAYFEDKFRF

Specific function: Catalyzes the attachment of glycine to tRNA(Gly)

COG id: COG0423

COG function: function code J; Glycyl-tRNA synthetase (class II)

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family

Homologues:

Organism=Homo sapiens, GI116805340, Length=379, Percent_Identity=30.8707124010554, Blast_Score=165, Evalue=8e-41,
Organism=Homo sapiens, GI70887790, Length=446, Percent_Identity=20.8520179372197, Blast_Score=75, Evalue=1e-13,
Organism=Caenorhabditis elegans, GI32564541, Length=399, Percent_Identity=29.5739348370927, Blast_Score=161, Evalue=9e-40,
Organism=Caenorhabditis elegans, GI17554996, Length=399, Percent_Identity=29.5739348370927, Blast_Score=160, Evalue=1e-39,
Organism=Saccharomyces cerevisiae, GI6325338, Length=366, Percent_Identity=29.7814207650273, Blast_Score=153, Evalue=7e-38,
Organism=Saccharomyces cerevisiae, GI6319597, Length=397, Percent_Identity=27.7078085642317, Blast_Score=148, Evalue=2e-36,
Organism=Drosophila melanogaster, GI21357965, Length=359, Percent_Identity=33.1476323119777, Blast_Score=176, Evalue=3e-44,
Organism=Drosophila melanogaster, GI24664462, Length=359, Percent_Identity=33.1476323119777, Blast_Score=176, Evalue=4e-44,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): SYG_EUBE2 (C4Z1I4)

Other databases:

- EMBL:   CP001104
- RefSeq:   YP_002930792.1
- GeneID:   7958205
- GenomeReviews:   CP001104_GR
- KEGG:   eel:EUBELI_01350
- OMA:   GSEWGEL
- ProtClustDB:   PRK04173
- GO:   GO:0005737
- HAMAP:   MF_00253_B
- InterPro:   IPR002314
- InterPro:   IPR006195
- InterPro:   IPR004154
- InterPro:   IPR022961
- InterPro:   IPR002315
- Gene3D:   G3DSA:3.40.50.800
- PANTHER:   PTHR10745
- PRINTS:   PR01043
- TIGRFAMs:   TIGR00389

Pfam domain/function: PF03129 HGTP_anticodon; PF00587 tRNA-synt_2b; SSF52954 Anticodon_bd

EC number: =6.1.1.14

Molecular weight: Translated: 53525; Mature: 53525

Theoretical pI: Translated: 4.67; Mature: 4.67

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: BINDING 98-98 BINDING 174-174

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.6 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
2.6 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEKTMEKIVSLAKARGFVYPGSEIYGGLANTWDYGNLGVELKNNVKKAWWQKFVQESPYN
CCHHHHHHHHHHHHCCCCCCCHHHHCCCCCCCCCCCCCEEHHHHHHHHHHHHHHHCCCCC
VGVDCAILMNPQTWVASGHLGGFSDPLMDCKECHERFRADKLIEDWADENSYDLGGSVDG
CCCEEEEEECCCCEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCC
WTQEQMKNFIDEKNICCPSCGKHNFTDIRQFNLMFKTFQGVTEDAKNTVYLRPETAQGIF
CCHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCEEE
VNFKNVQRTSRKKVPFGIGQIGKSFRNEITPGNFTFRTREFEQMELEFFCKPGTDLEWFT
EEEHHHHHHHCCCCCCCHHHHHHHHHHCCCCCCEEEECCHHHHEEEEEEECCCCCHHHHH
YWRQYCIDWLKALGIKEDEMRARDHSPEELCFYSKGTTDIEFLFPFGWGELWGIADRTDY
HHHHHHHHHHHHCCCCHHHHHHCCCCHHHEEEECCCCCCEEEEEECCCHHHCCCCCCCCC
DLTQHQTVSGEDMSYFDDEAKEKYIPYVIEPSLGADRVTLAFLCSAYDEEELEGGDVRTV
CCCCCCCCCCCCCHHHCCHHHHCCCCEEECCCCCCCHHHHHHHHHHCCHHHCCCCCEEEE
LHFHPAIAPVKIGILPLSKKLNEGAEKVYAELSKYYNCEFDDRGNIGKRYRRQDEIGTPF
EEECCCCCEEEEEEEECHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHCCCCCE
CITYDFDSEEDGAVTVRDRDTMQQERIKIADLKAYFEDKFRF
EEEECCCCCCCCEEEEECHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MEKTMEKIVSLAKARGFVYPGSEIYGGLANTWDYGNLGVELKNNVKKAWWQKFVQESPYN
CCHHHHHHHHHHHHCCCCCCCHHHHCCCCCCCCCCCCCEEHHHHHHHHHHHHHHHCCCCC
VGVDCAILMNPQTWVASGHLGGFSDPLMDCKECHERFRADKLIEDWADENSYDLGGSVDG
CCCEEEEEECCCCEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCC
WTQEQMKNFIDEKNICCPSCGKHNFTDIRQFNLMFKTFQGVTEDAKNTVYLRPETAQGIF
CCHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCEEE
VNFKNVQRTSRKKVPFGIGQIGKSFRNEITPGNFTFRTREFEQMELEFFCKPGTDLEWFT
EEEHHHHHHHCCCCCCCHHHHHHHHHHCCCCCCEEEECCHHHHEEEEEEECCCCCHHHHH
YWRQYCIDWLKALGIKEDEMRARDHSPEELCFYSKGTTDIEFLFPFGWGELWGIADRTDY
HHHHHHHHHHHHCCCCHHHHHHCCCCHHHEEEECCCCCCEEEEEECCCHHHCCCCCCCCC
DLTQHQTVSGEDMSYFDDEAKEKYIPYVIEPSLGADRVTLAFLCSAYDEEELEGGDVRTV
CCCCCCCCCCCCCHHHCCHHHHCCCCEEECCCCCCCHHHHHHHHHHCCHHHCCCCCEEEE
LHFHPAIAPVKIGILPLSKKLNEGAEKVYAELSKYYNCEFDDRGNIGKRYRRQDEIGTPF
EEECCCCCEEEEEEEECHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHCCCCCE
CITYDFDSEEDGAVTVRDRDTMQQERIKIADLKAYFEDKFRF
EEEECCCCCCCCEEEEECHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA