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The map label for this gene is pyrB [H]

Identifier: 238917260

GI number: 238917260

Start: 1367245

End: 1368207

Strand: Reverse

Name: pyrB [H]

Synonym: EUBELI_01334

Alternate gene names: 238917260

Gene position: 1368207-1367245 (Counterclockwise)

Preceding gene: 238917261

Following gene: 238917259

Centisome position: 63.81

GC content: 37.9

Gene sequence:

>963_bases
ATGAGACATTTACTTAGTCCGCTTGATTTAAGCGTTGATGAACTGGCAAGATTATTAGATCTTGCAAGAGACATTTCAAA
GGATCCATCAAAGTATGGTCATGTATGTGATGGAAAGAAGATAGCCACATTGTTCTATGAGCCAAGTACTAGAACAAGAT
TAAGCTTCGAGGCTGCCATGATTAACCTTGGCGGTCAGGTATTAGGCTTTTCTGAGGCTTCTTCAAGTTCAGCGTCAAAG
GGTGAAAGTGTTGCAGATACTATAAGAGTTATCTCTTGTTATGCAGATATATGTGCAATGCGTCATCCAAAGGAAGGTGC
TCCAATGGTAGCTTCTGAATATTCGCACATTCCTATTATCAATGCAGGTGATGGAGGACATCAGCATCCTACACAGACAC
TTACAGATCTTATGACAATAAGGGAACTTCGTGGTTCGCTTGATAATTTTACAATAGGATTATGTGGTGACCTTAAGTTC
GGAAGAACAGTTCACTCACTTATAAGCTCATTAGTAAGATATAAGAATGTAAAGTTTGTCCTTATATCACCTAAGGAGTT
AAGAATTCCTGATTATATCAGGGATGATGTACTTAAGGCTAATAACTGTGATTTCGTAGAAGTTGAGAACCTTGAGCAGG
CTATGCCAGAACTTGATATTCTCTATATGACAAGAGTACAGAGAGAAAGATTTTTCAGCGAAGATGAGTACTTAAGAATG
AAGGACTTCTATATACTGGATGAAGATAAGATGGCACTTGGAAAGCCTGATATGTATGTTCTTCATCCGCTTCCAAGAGT
TAATGAAATATCTGTTGATGTTGACAATGATGACAGAGCTGCTTATTTCAAGCAGGTTCAGTTTGGTGTATATGTAAGAA
TGGCACTTATACTTACTTTACTTGGACTTGATACAGTGGTTAATCCATCATTAGCTCATGTACTTGATTCACTGGATGCA
TAA

Upstream 100 bases:

>100_bases
ATTATTTTAATGATGCAGAACTTATTAAGGTTCAGTCAGAAAAATACAGCAGAACAGAGTAATTATTTTAATTAAATATA
CACACGGAGGCTTTTCTATT

Downstream 100 bases:

>100_bases
GGAGGAAAATTTATGTTAAATGTAGGCGAACTTAATGAAGGCTTTGTTCTTGACCATATTGAAGCAGGAAAGGCCATGAT
GATATACAATTACTTAGGAC

Product: aspartate carbamoyltransferase catalytic subunit

Products: NA

Alternate protein names: Aspartate transcarbamylase; ATCase [H]

Number of amino acids: Translated: 320; Mature: 320

Protein sequence:

>320_residues
MRHLLSPLDLSVDELARLLDLARDISKDPSKYGHVCDGKKIATLFYEPSTRTRLSFEAAMINLGGQVLGFSEASSSSASK
GESVADTIRVISCYADICAMRHPKEGAPMVASEYSHIPIINAGDGGHQHPTQTLTDLMTIRELRGSLDNFTIGLCGDLKF
GRTVHSLISSLVRYKNVKFVLISPKELRIPDYIRDDVLKANNCDFVEVENLEQAMPELDILYMTRVQRERFFSEDEYLRM
KDFYILDEDKMALGKPDMYVLHPLPRVNEISVDVDNDDRAAYFKQVQFGVYVRMALILTLLGLDTVVNPSLAHVLDSLDA

Sequences:

>Translated_320_residues
MRHLLSPLDLSVDELARLLDLARDISKDPSKYGHVCDGKKIATLFYEPSTRTRLSFEAAMINLGGQVLGFSEASSSSASK
GESVADTIRVISCYADICAMRHPKEGAPMVASEYSHIPIINAGDGGHQHPTQTLTDLMTIRELRGSLDNFTIGLCGDLKF
GRTVHSLISSLVRYKNVKFVLISPKELRIPDYIRDDVLKANNCDFVEVENLEQAMPELDILYMTRVQRERFFSEDEYLRM
KDFYILDEDKMALGKPDMYVLHPLPRVNEISVDVDNDDRAAYFKQVQFGVYVRMALILTLLGLDTVVNPSLAHVLDSLDA
>Mature_320_residues
MRHLLSPLDLSVDELARLLDLARDISKDPSKYGHVCDGKKIATLFYEPSTRTRLSFEAAMINLGGQVLGFSEASSSSASK
GESVADTIRVISCYADICAMRHPKEGAPMVASEYSHIPIINAGDGGHQHPTQTLTDLMTIRELRGSLDNFTIGLCGDLKF
GRTVHSLISSLVRYKNVKFVLISPKELRIPDYIRDDVLKANNCDFVEVENLEQAMPELDILYMTRVQRERFFSEDEYLRM
KDFYILDEDKMALGKPDMYVLHPLPRVNEISVDVDNDDRAAYFKQVQFGVYVRMALILTLLGLDTVVNPSLAHVLDSLDA

Specific function: Pyrimidine biosynthesis; second step. [C]

COG id: COG0540

COG function: function code F; Aspartate carbamoyltransferase, catalytic chain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATCase/OTCase family [H]

Homologues:

Organism=Homo sapiens, GI18105007, Length=303, Percent_Identity=46.8646864686469, Blast_Score=273, Evalue=2e-73,
Organism=Homo sapiens, GI38788445, Length=310, Percent_Identity=26.7741935483871, Blast_Score=77, Evalue=3e-14,
Organism=Escherichia coli, GI2367364, Length=308, Percent_Identity=45.7792207792208, Blast_Score=270, Evalue=1e-73,
Organism=Escherichia coli, GI1790703, Length=309, Percent_Identity=27.1844660194175, Blast_Score=74, Evalue=9e-15,
Organism=Escherichia coli, GI1786469, Length=163, Percent_Identity=35.5828220858896, Blast_Score=74, Evalue=2e-14,
Organism=Caenorhabditis elegans, GI193204318, Length=319, Percent_Identity=39.1849529780564, Blast_Score=214, Evalue=4e-56,
Organism=Saccharomyces cerevisiae, GI6322331, Length=305, Percent_Identity=44.2622950819672, Blast_Score=263, Evalue=2e-71,
Organism=Saccharomyces cerevisiae, GI6322373, Length=318, Percent_Identity=25.4716981132075, Blast_Score=70, Evalue=6e-13,
Organism=Drosophila melanogaster, GI24642586, Length=304, Percent_Identity=42.4342105263158, Blast_Score=239, Evalue=2e-63,

Paralogues:

None

Copy number: 2080 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 14328 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006132
- InterPro:   IPR006130
- InterPro:   IPR006131
- InterPro:   IPR002082 [H]

Pfam domain/function: PF00185 OTCace; PF02729 OTCace_N [H]

EC number: =2.1.3.2 [H]

Molecular weight: Translated: 35938; Mature: 35938

Theoretical pI: Translated: 5.11; Mature: 5.11

Prosite motif: PS00097 CARBAMOYLTRANSFERASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRHLLSPLDLSVDELARLLDLARDISKDPSKYGHVCDGKKIATLFYEPSTRTRLSFEAAM
CCCCCCCCCCCHHHHHHHHHHHHHHCCCHHHHCCCCCCCEEEEEEECCCCCCEEEHHHHH
INLGGQVLGFSEASSSSASKGESVADTIRVISCYADICAMRHPKEGAPMVASEYSHIPII
HHCCCCEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHCCCCCCCEE
NAGDGGHQHPTQTLTDLMTIRELRGSLDNFTIGLCGDLKFGRTVHSLISSLVRYKNVKFV
ECCCCCCCCHHHHHHHHHHHHHHHCCCCCEEEEEECCCCHHHHHHHHHHHHHHHCCCEEE
LISPKELRIPDYIRDDVLKANNCDFVEVENLEQAMPELDILYMTRVQRERFFSEDEYLRM
EECCCCCCCCHHHHHHHHCCCCCCEEEECCHHHHCCCHHHHHHHHHHHHHHCCCCCCEEE
KDFYILDEDKMALGKPDMYVLHPLPRVNEISVDVDNDDRAAYFKQVQFGVYVRMALILTL
EEEEEECCCCCCCCCCCEEEECCCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHH
LGLDTVVNPSLAHVLDSLDA
HHHHHHCCHHHHHHHHHHCC
>Mature Secondary Structure
MRHLLSPLDLSVDELARLLDLARDISKDPSKYGHVCDGKKIATLFYEPSTRTRLSFEAAM
CCCCCCCCCCCHHHHHHHHHHHHHHCCCHHHHCCCCCCCEEEEEEECCCCCCEEEHHHHH
INLGGQVLGFSEASSSSASKGESVADTIRVISCYADICAMRHPKEGAPMVASEYSHIPII
HHCCCCEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHCCCCCCCEE
NAGDGGHQHPTQTLTDLMTIRELRGSLDNFTIGLCGDLKFGRTVHSLISSLVRYKNVKFV
ECCCCCCCCHHHHHHHHHHHHHHHCCCCCEEEEEECCCCHHHHHHHHHHHHHHHCCCEEE
LISPKELRIPDYIRDDVLKANNCDFVEVENLEQAMPELDILYMTRVQRERFFSEDEYLRM
EECCCCCCCCHHHHHHHHCCCCCCEEEECCHHHHCCCHHHHHHHHHHHHHHCCCCCCEEE
KDFYILDEDKMALGKPDMYVLHPLPRVNEISVDVDNDDRAAYFKQVQFGVYVRMALILTL
EEEEEECCCCCCCCCCCEEEECCCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHH
LGLDTVVNPSLAHVLDSLDA
HHHHHHCCHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA