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The map label for this gene is trpA

Identifier: 238917228

GI number: 238917228

Start: 1338629

End: 1339423

Strand: Reverse

Name: trpA

Synonym: EUBELI_01301

Alternate gene names: 238917228

Gene position: 1339423-1338629 (Counterclockwise)

Preceding gene: 238917229

Following gene: 238917227

Centisome position: 62.47

GC content: 38.74

Gene sequence:

>795_bases
ATGAATAGAATAGAAGAAAGACTTGCAGCACTTAAAGAAGAGGGAAAGAAAGCATTTATTACATATACAACAGCAGGACT
TCCAGATTACGATACAACTAAGAAGATTATGTTTGCACAGGAGAAGGCAGGAATCGATATTATGGAAATAGGTGTTCCTT
TCTCAGATCCTATTGCAGATGGCCCTGTAATTCAGGATGCATCTTTCAAAGCAATACAGAATGGAGCTTCACTTGAAGGC
ATATTTACAATGATGGGTAAGGTAAGAAAAGCTGGTCTTAACAGCCCTGTTGTATTCATGCTTTACTATAATACTGTATA
TCATTATGGAGTTGAGAACTTCGTGAATAAATGTATTGAAAATGGTGTTGACGGACTTATCATACCTGACCTTCCATTCG
AGGAGCAGGGCGAGATTAAGGATGTCCTTGCAAAGAACGAGAAGTCGCCAATTCTTATCCAGCTTGTATCTCCGGTATCT
AAGGGCAGGATTCCAATGCTTTTAGAGAACGCAAGAGGTTTTGTATACTGCGTTTCACAGATGGGTGTTACAGGAAAGGG
AGCTAACTTCCATTCACAGATAAGAGAATATCTTACAGAAGTTAAGAAAGAATCTAAGATTCCTGTTATGATGGGATTCG
GAATAAGAACAGCAGCAGATGTTGCACCGCTTGAAGATATTATTGATGGTGCAATCGTTGGTTCACATTTTATCAAGCTT
CTTGAAGCTAATAATTATTCAGAGGAAGCTGCCACAGATTATGTTGCAACATTTAAGAAGGAGCTTAATGCCTGA

Upstream 100 bases:

>100_bases
AATATAAGGGTAAGACAATTATCATGTGTCTTTCAGGCCGTGGTGATAAGGATGTTAATACAGTTCAGAAATATCTTAAC
GGAGAGGAGACTAACAAGTA

Downstream 100 bases:

>100_bases
TGGTACCGATATTAGCTAAGCAAGCGCTTGCGCGCAAATGTGAATTTTCTAATGCAAGAATGACTGGCAATTATGAATGT
GCCTATGCTCTTGGAGTTGT

Product: tryptophan synthase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 264; Mature: 264

Protein sequence:

>264_residues
MNRIEERLAALKEEGKKAFITYTTAGLPDYDTTKKIMFAQEKAGIDIMEIGVPFSDPIADGPVIQDASFKAIQNGASLEG
IFTMMGKVRKAGLNSPVVFMLYYNTVYHYGVENFVNKCIENGVDGLIIPDLPFEEQGEIKDVLAKNEKSPILIQLVSPVS
KGRIPMLLENARGFVYCVSQMGVTGKGANFHSQIREYLTEVKKESKIPVMMGFGIRTAADVAPLEDIIDGAIVGSHFIKL
LEANNYSEEAATDYVATFKKELNA

Sequences:

>Translated_264_residues
MNRIEERLAALKEEGKKAFITYTTAGLPDYDTTKKIMFAQEKAGIDIMEIGVPFSDPIADGPVIQDASFKAIQNGASLEG
IFTMMGKVRKAGLNSPVVFMLYYNTVYHYGVENFVNKCIENGVDGLIIPDLPFEEQGEIKDVLAKNEKSPILIQLVSPVS
KGRIPMLLENARGFVYCVSQMGVTGKGANFHSQIREYLTEVKKESKIPVMMGFGIRTAADVAPLEDIIDGAIVGSHFIKL
LEANNYSEEAATDYVATFKKELNA
>Mature_264_residues
MNRIEERLAALKEEGKKAFITYTTAGLPDYDTTKKIMFAQEKAGIDIMEIGVPFSDPIADGPVIQDASFKAIQNGASLEG
IFTMMGKVRKAGLNSPVVFMLYYNTVYHYGVENFVNKCIENGVDGLIIPDLPFEEQGEIKDVLAKNEKSPILIQLVSPVS
KGRIPMLLENARGFVYCVSQMGVTGKGANFHSQIREYLTEVKKESKIPVMMGFGIRTAADVAPLEDIIDGAIVGSHFIKL
LEANNYSEEAATDYVATFKKELNA

Specific function: The alpha subunit is responsible for the aldol cleavage of indoleglycerol phosphate to indole and glyceraldehyde 3- phosphate

COG id: COG0159

COG function: function code E; Tryptophan synthase alpha chain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the trpA family

Homologues:

Organism=Escherichia coli, GI1787514, Length=216, Percent_Identity=31.4814814814815, Blast_Score=130, Evalue=1e-31,
Organism=Saccharomyces cerevisiae, GI6321412, Length=259, Percent_Identity=35.9073359073359, Blast_Score=166, Evalue=5e-42,

Paralogues:

None

Copy number: 280 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 3,500 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): TRPA_EUBE2 (C4Z135)

Other databases:

- EMBL:   CP001104
- RefSeq:   YP_002930745.1
- GeneID:   7956647
- GenomeReviews:   CP001104_GR
- KEGG:   eel:EUBELI_01301
- OMA:   NPIYIYG
- HAMAP:   MF_00131
- InterPro:   IPR013785
- InterPro:   IPR011060
- InterPro:   IPR018204
- InterPro:   IPR002028
- Gene3D:   G3DSA:3.20.20.70
- TIGRFAMs:   TIGR00262

Pfam domain/function: PF00290 Trp_syntA; SSF51366 RibP_bind_barrel

EC number: =4.2.1.20

Molecular weight: Translated: 29044; Mature: 29044

Theoretical pI: Translated: 4.92; Mature: 4.92

Prosite motif: PS00167 TRP_SYNTHASE_ALPHA

Important sites: ACT_SITE 49-49 ACT_SITE 60-60

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNRIEERLAALKEEGKKAFITYTTAGLPDYDTTKKIMFAQEKAGIDIMEIGVPFSDPIAD
CCHHHHHHHHHHHCCCEEEEEEEECCCCCCCCCHHHEEEHHHCCCEEEEECCCCCCCCCC
GPVIQDASFKAIQNGASLEGIFTMMGKVRKAGLNSPVVFMLYYNTVYHYGVENFVNKCIE
CCEECCCCHHHHHCCCCHHHHHHHHHHHHHCCCCCCEEEEEEHHHHHHHHHHHHHHHHHH
NGVDGLIIPDLPFEEQGEIKDVLAKNEKSPILIQLVSPVSKGRIPMLLENARGFVYCVSQ
CCCCEEEECCCCCCCCCCHHHHHHCCCCCCEEEEEECCCCCCCCCEEEECCCCEEEEHHH
MGVTGKGANFHSQIREYLTEVKKESKIPVMMGFGIRTAADVAPLEDIIDGAIVGSHFIKL
CCCCCCCCCHHHHHHHHHHHHHHHCCCCEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHH
LEANNYSEEAATDYVATFKKELNA
HHCCCCCHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MNRIEERLAALKEEGKKAFITYTTAGLPDYDTTKKIMFAQEKAGIDIMEIGVPFSDPIAD
CCHHHHHHHHHHHCCCEEEEEEEECCCCCCCCCHHHEEEHHHCCCEEEEECCCCCCCCCC
GPVIQDASFKAIQNGASLEGIFTMMGKVRKAGLNSPVVFMLYYNTVYHYGVENFVNKCIE
CCEECCCCHHHHHCCCCHHHHHHHHHHHHHCCCCCCEEEEEEHHHHHHHHHHHHHHHHHH
NGVDGLIIPDLPFEEQGEIKDVLAKNEKSPILIQLVSPVSKGRIPMLLENARGFVYCVSQ
CCCCEEEECCCCCCCCCCHHHHHHCCCCCCEEEEEECCCCCCCCCEEEECCCCEEEEHHH
MGVTGKGANFHSQIREYLTEVKKESKIPVMMGFGIRTAADVAPLEDIIDGAIVGSHFIKL
CCCCCCCCCHHHHHHHHHHHHHHHCCCCEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHH
LEANNYSEEAATDYVATFKKELNA
HHCCCCCHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA