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The map label for this gene is folA2 [H]

Identifier: 238917197

GI number: 238917197

Start: 1306218

End: 1306706

Strand: Reverse

Name: folA2 [H]

Synonym: EUBELI_01270

Alternate gene names: 238917197

Gene position: 1306706-1306218 (Counterclockwise)

Preceding gene: 238917198

Following gene: 238917196

Centisome position: 60.94

GC content: 36.4

Gene sequence:

>489_bases
ATGAATCTGATAGCAACAGTAGATAAGAACTGGTCAATAGGAAAACAGGGACATCTTCTTGTTAATATTCCGGAAGATAT
TAAGCTTTTCCGAATGGAAACAGCTGGAAAAGCTGCAATAATGGGAAGAAAGACATATGAGCAGATTACGGACAGAACAG
CTCTTATAGACAGATATAATGTAGTGCTTACTAGTGATTTGTCATATAAGAAGGATGGAGCTGTTGTAGTGCATTCAGTA
GAAGAGGCACTTGAAAAGGTTGCATCATATAAGTCAGAGGATATATATGTAATCGGTGGAGAATCTGTATTTGAACAGTT
TCTTCCATATTGCGATGTGGCACACATAACGAGTGTTGATTATAAGTATGATGCAGATGCACATTTTCCTAATCTGGATA
AGATGCCAGAATGGAAAGTTACACAGATGAGTGAGGAACACACATACTTTGATTTGTGCTACGAATTTAAAAAGTACTGT
AAAATGTAA

Upstream 100 bases:

>100_bases
TTATAAGACAGTTCGGACAGCTTGAAAGTGAAGAGTACACAGAAGACGGAATTAAGGTGCTTGCCTATGTTCCTAAGGAG
ATAGAAGGGAAGTTGTAATT

Downstream 100 bases:

>100_bases
ATTATGTTTTGAATCGTGCAGAAACGGATATGAAAGAGCAGTTTGTGGCATATTTTTTTCATTGAAAATATACATTTTAT
TTGATACTATAATTATAAAA

Product: dihydrofolate reductase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 162; Mature: 162

Protein sequence:

>162_residues
MNLIATVDKNWSIGKQGHLLVNIPEDIKLFRMETAGKAAIMGRKTYEQITDRTALIDRYNVVLTSDLSYKKDGAVVVHSV
EEALEKVASYKSEDIYVIGGESVFEQFLPYCDVAHITSVDYKYDADAHFPNLDKMPEWKVTQMSEEHTYFDLCYEFKKYC
KM

Sequences:

>Translated_162_residues
MNLIATVDKNWSIGKQGHLLVNIPEDIKLFRMETAGKAAIMGRKTYEQITDRTALIDRYNVVLTSDLSYKKDGAVVVHSV
EEALEKVASYKSEDIYVIGGESVFEQFLPYCDVAHITSVDYKYDADAHFPNLDKMPEWKVTQMSEEHTYFDLCYEFKKYC
KM
>Mature_162_residues
MNLIATVDKNWSIGKQGHLLVNIPEDIKLFRMETAGKAAIMGRKTYEQITDRTALIDRYNVVLTSDLSYKKDGAVVVHSV
EEALEKVASYKSEDIYVIGGESVFEQFLPYCDVAHITSVDYKYDADAHFPNLDKMPEWKVTQMSEEHTYFDLCYEFKKYC
KM

Specific function: Key enzyme in folate metabolism. Catalyzes an essential reaction for de novo glycine and purine synthesis, and for DNA precursor synthesis [H]

COG id: COG0262

COG function: function code H; Dihydrofolate reductase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 DHFR (dihydrofolate reductase) domain [H]

Homologues:

Organism=Escherichia coli, GI1786233, Length=131, Percent_Identity=35.8778625954198, Blast_Score=78, Evalue=3e-16,
Organism=Caenorhabditis elegans, GI17505899, Length=180, Percent_Identity=31.6666666666667, Blast_Score=69, Evalue=1e-12,
Organism=Drosophila melanogaster, GI116008026, Length=144, Percent_Identity=31.9444444444444, Blast_Score=70, Evalue=6e-13,
Organism=Drosophila melanogaster, GI24647458, Length=144, Percent_Identity=31.9444444444444, Blast_Score=70, Evalue=6e-13,

Paralogues:

None

Copy number: 640 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 10,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012259
- InterPro:   IPR017925
- InterPro:   IPR001796 [H]

Pfam domain/function: PF00186 DHFR_1 [H]

EC number: =1.5.1.3 [H]

Molecular weight: Translated: 18683; Mature: 18683

Theoretical pI: Translated: 5.06; Mature: 5.06

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
5.6 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
5.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNLIATVDKNWSIGKQGHLLVNIPEDIKLFRMETAGKAAIMGRKTYEQITDRTALIDRYN
CCEEEEECCCCCCCCCCCEEEECCCCCEEEEECCCCCEEECCCHHHHHHHHHHHHHHHEE
VVLTSDLSYKKDGAVVVHSVEEALEKVASYKSEDIYVIGGESVFEQFLPYCDVAHITSVD
EEEECCCCCCCCCEEEEHHHHHHHHHHHCCCCCCEEEECCHHHHHHHCCCCCHHHHEECC
YKYDADAHFPNLDKMPEWKVTQMSEEHTYFDLCYEFKKYCKM
CEECCCCCCCCCCCCCCCCEECCCCCCHHHHHHHHHHHHHCC
>Mature Secondary Structure
MNLIATVDKNWSIGKQGHLLVNIPEDIKLFRMETAGKAAIMGRKTYEQITDRTALIDRYN
CCEEEEECCCCCCCCCCCEEEECCCCCEEEEECCCCCEEECCCHHHHHHHHHHHHHHHEE
VVLTSDLSYKKDGAVVVHSVEEALEKVASYKSEDIYVIGGESVFEQFLPYCDVAHITSVD
EEEECCCCCCCCCEEEEHHHHHHHHHHHCCCCCCEEEECCHHHHHHHCCCCCHHHHEECC
YKYDADAHFPNLDKMPEWKVTQMSEEHTYFDLCYEFKKYCKM
CEECCCCCCCCCCCCCCCCEECCCCCCHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA