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The map label for this gene is greA [H]

Identifier: 238917160

GI number: 238917160

Start: 1251270

End: 1251770

Strand: Reverse

Name: greA [H]

Synonym: EUBELI_01232

Alternate gene names: 238917160

Gene position: 1251770-1251270 (Counterclockwise)

Preceding gene: 238917161

Following gene: 238917159

Centisome position: 58.38

GC content: 37.72

Gene sequence:

>501_bases
ATGGGACACGAACTTACTAAGAATGATATCAAAAAGATGGAAGAAGAGATTGAGTACAGGATACATGAGGTAAGAAAGGC
TGCACTTGATGATGTCAAGGAGACAAGGGCTCAGGGTGATTTAAGTGAGAATTTCGAGTATCATGCAGCGAAAAGGTTTA
AGAATAAGAACGAGAGTAGAATAAGATACCTGCAGAGAACTATTAAGAATGCGACTGTTATTGATGATTCATCAGCAGAT
GATGAGGTTGGTGTTAATAATACGGTTGAGGTATTGTATGAGGATGATGAAAGCACTGAGACTATTAAGATTGTTACTAC
AATCAGGGCTGATTCGTTAAGCGGAATGATCAGTATAGAGTCGCCTCTCGGCAAGGCTCTTCTTGGACATAAGAAGGGTG
ACAGGGTTCTTGTACAGGTTAATCCACAGGTTGAATATTATGTTGTTATCAAGGATATATCTAATACTGATGATTCTGAT
GATAATATAACAAGTTATTAA

Upstream 100 bases:

>100_bases
ACTTAAAGTCGAGACAGAATAGACTTGCAAAATGTATCTTAAAAGGGCAAAATATACTCATATTATTTCATTGATTAAAA
GGTAAGAAAGAAGGATAGAT

Downstream 100 bases:

>100_bases
TAAAATATAAAATATATATTATTGAATTGACATAATAGCGACATACTTATATAATCTTAGTATACATTTGTTTGTGTAAG
ATTATTATAAGGGGTGCCGC

Product: transcription elongation factor GreA

Products: NA

Alternate protein names: Transcript cleavage factor greA [H]

Number of amino acids: Translated: 166; Mature: 165

Protein sequence:

>166_residues
MGHELTKNDIKKMEEEIEYRIHEVRKAALDDVKETRAQGDLSENFEYHAAKRFKNKNESRIRYLQRTIKNATVIDDSSAD
DEVGVNNTVEVLYEDDESTETIKIVTTIRADSLSGMISIESPLGKALLGHKKGDRVLVQVNPQVEYYVVIKDISNTDDSD
DNITSY

Sequences:

>Translated_166_residues
MGHELTKNDIKKMEEEIEYRIHEVRKAALDDVKETRAQGDLSENFEYHAAKRFKNKNESRIRYLQRTIKNATVIDDSSAD
DEVGVNNTVEVLYEDDESTETIKIVTTIRADSLSGMISIESPLGKALLGHKKGDRVLVQVNPQVEYYVVIKDISNTDDSD
DNITSY
>Mature_165_residues
GHELTKNDIKKMEEEIEYRIHEVRKAALDDVKETRAQGDLSENFEYHAAKRFKNKNESRIRYLQRTIKNATVIDDSSADD
EVGVNNTVEVLYEDDESTETIKIVTTIRADSLSGMISIESPLGKALLGHKKGDRVLVQVNPQVEYYVVIKDISNTDDSDD
NITSY

Specific function: Necessary for efficient RNA polymerase transcription elongation past template-encoded arresting sites. The arresting sites in DNA have the property of trapping a certain fraction of elongating RNA polymerases that pass through, resulting in locked ternary

COG id: COG0782

COG function: function code K; Transcription elongation factor

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the greA/greB family [H]

Homologues:

Organism=Escherichia coli, GI87082225, Length=148, Percent_Identity=31.7567567567568, Blast_Score=70, Evalue=7e-14,

Paralogues:

None

Copy number: 259 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018151
- InterPro:   IPR006359
- InterPro:   IPR001437
- InterPro:   IPR022691 [H]

Pfam domain/function: PF01272 GreA_GreB; PF03449 GreA_GreB_N [H]

EC number: NA

Molecular weight: Translated: 18896; Mature: 18765

Theoretical pI: Translated: 4.60; Mature: 4.60

Prosite motif: PS00830 GREAB_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
1.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGHELTKNDIKKMEEEIEYRIHEVRKAALDDVKETRAQGDLSENFEYHAAKRFKNKNESR
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHCCCCHHH
IRYLQRTIKNATVIDDSSADDEVGVNNTVEVLYEDDESTETIKIVTTIRADSLSGMISIE
HHHHHHHHCCCEEECCCCCCCCCCCCCEEEEEECCCCCCCEEEEEEEEECCCCCCEEEEC
SPLGKALLGHKKGDRVLVQVNPQVEYYVVIKDISNTDDSDDNITSY
CCHHHHHHCCCCCCEEEEEECCCEEEEEEEEECCCCCCCCCCCCCC
>Mature Secondary Structure 
GHELTKNDIKKMEEEIEYRIHEVRKAALDDVKETRAQGDLSENFEYHAAKRFKNKNESR
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHCCCCHHH
IRYLQRTIKNATVIDDSSADDEVGVNNTVEVLYEDDESTETIKIVTTIRADSLSGMISIE
HHHHHHHHCCCEEECCCCCCCCCCCCCEEEEEECCCCCCCEEEEEEEEECCCCCCEEEEC
SPLGKALLGHKKGDRVLVQVNPQVEYYVVIKDISNTDDSDDNITSY
CCHHHHHHCCCCCCEEEEEECCCEEEEEEEEECCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA