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The map label for this gene is ybjI [H]

Identifier: 238917151

GI number: 238917151

Start: 1239489

End: 1240271

Strand: Reverse

Name: ybjI [H]

Synonym: EUBELI_01223

Alternate gene names: 238917151

Gene position: 1240271-1239489 (Counterclockwise)

Preceding gene: 238917152

Following gene: 238917150

Centisome position: 57.84

GC content: 36.53

Gene sequence:

>783_bases
ATGGTTAAATTCGTAGCAAGTGACCTTGATGGGACATTGCTTCTAAATGGAGCGCAGAGTGTTGATGAATCTGCGGTACA
GTATATTAATAAGCTTGTTGATAATGGAGTTATATTTGCACCGGCTAGTGGCAGGCAGATAACGAGTCTTAAGAGACTTT
TTGGAGTTGTCAGTGATAGGCTTGCATATATTGCGGAGAATGGTGCTCTTGTAGAATACAGGGGAAAAACTATTGGTAAG
ACTCCAATGGAGCGGAAACTTGCATTAGAGATAATTGAGGATGTAATTGAACAGCCAAATTGTGAGGTGCTTGTATCAGG
AGAACATACGGCATATATTAAACCTAAGTCAGAAGAATATTATTACAGAATGACCAAGGTGGTCAATTACCATACAACAT
TAGTCGATAAGTTTACAGACATTGATGAGGATATATTAAAGATAGCTGTATGTGATATGACAGGAATTAAGAACTCTAAA
GAGCATTTTATTAACAAATGGAGTGATAAGGCTTCAGTACTTGTATCGGGAGAACTTTACTTAGACCTTATGGACACTAA
TGTTAATAAAGGAAGAGGAATTGAGCAGGTTCAGCAGTATTTTGGACTAAAACCTGAAGAATGTATGGCATTTGGTGATA
ACTATAATGATATTGCAATGCTTGACAATGTGTATTACTCATATGTTATGGAGAAAGCTGTAGATGATGTTAAGAAACAT
GGGCGTTTCATAACAGGATGGGTAGAAGGAACATTAAGAGAACAATTTAAGGAGATAAGATAA

Upstream 100 bases:

>100_bases
CAGCATACATTAAGGGTATTGAAGGCGACTATAATAATGTTGTCGGTCTGCCTGTAGCAAGACTTGTACATGAGCTTAAA
GTATTTTAAGGAGAAAATAT

Downstream 100 bases:

>100_bases
GGAGACTTAAGTTATGGATAAGCATATTGATGAATGCGCAAAGGTATTTTTAAGAGACCAGAAACAGCTTTTTGATGAGC
CTGTTGCATATGATTTAGAC

Product: hypothetical protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 260; Mature: 260

Protein sequence:

>260_residues
MVKFVASDLDGTLLLNGAQSVDESAVQYINKLVDNGVIFAPASGRQITSLKRLFGVVSDRLAYIAENGALVEYRGKTIGK
TPMERKLALEIIEDVIEQPNCEVLVSGEHTAYIKPKSEEYYYRMTKVVNYHTTLVDKFTDIDEDILKIAVCDMTGIKNSK
EHFINKWSDKASVLVSGELYLDLMDTNVNKGRGIEQVQQYFGLKPEECMAFGDNYNDIAMLDNVYYSYVMEKAVDDVKKH
GRFITGWVEGTLREQFKEIR

Sequences:

>Translated_260_residues
MVKFVASDLDGTLLLNGAQSVDESAVQYINKLVDNGVIFAPASGRQITSLKRLFGVVSDRLAYIAENGALVEYRGKTIGK
TPMERKLALEIIEDVIEQPNCEVLVSGEHTAYIKPKSEEYYYRMTKVVNYHTTLVDKFTDIDEDILKIAVCDMTGIKNSK
EHFINKWSDKASVLVSGELYLDLMDTNVNKGRGIEQVQQYFGLKPEECMAFGDNYNDIAMLDNVYYSYVMEKAVDDVKKH
GRFITGWVEGTLREQFKEIR
>Mature_260_residues
MVKFVASDLDGTLLLNGAQSVDESAVQYINKLVDNGVIFAPASGRQITSLKRLFGVVSDRLAYIAENGALVEYRGKTIGK
TPMERKLALEIIEDVIEQPNCEVLVSGEHTAYIKPKSEEYYYRMTKVVNYHTTLVDKFTDIDEDILKIAVCDMTGIKNSK
EHFINKWSDKASVLVSGELYLDLMDTNVNKGRGIEQVQQYFGLKPEECMAFGDNYNDIAMLDNVYYSYVMEKAVDDVKKH
GRFITGWVEGTLREQFKEIR

Specific function: Catalyzes the dephosphorylation of the artificial chromogenic substrate p-nitrophenyl phosphate (pNPP) and of the natural substrates FMN and beta-glucose 1-phosphate [H]

COG id: COG0561

COG function: function code R; Predicted hydrolases of the HAD superfamily

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the HAD-like hydrolase superfamily. Cof family [H]

Homologues:

Organism=Escherichia coli, GI87081790, Length=246, Percent_Identity=24.7967479674797, Blast_Score=97, Evalue=1e-21,
Organism=Escherichia coli, GI1787043, Length=254, Percent_Identity=26.7716535433071, Blast_Score=92, Evalue=3e-20,
Organism=Escherichia coli, GI2367265, Length=263, Percent_Identity=26.9961977186312, Blast_Score=73, Evalue=2e-14,
Organism=Escherichia coli, GI48994981, Length=242, Percent_Identity=27.2727272727273, Blast_Score=66, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005834
- InterPro:   IPR023214
- InterPro:   IPR006379
- InterPro:   IPR000150 [H]

Pfam domain/function: PF00702 Hydrolase [H]

EC number: NA

Molecular weight: Translated: 29484; Mature: 29484

Theoretical pI: Translated: 4.86; Mature: 4.86

Prosite motif: PS01228 COF_1 ; PS01229 COF_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVKFVASDLDGTLLLNGAQSVDESAVQYINKLVDNGVIFAPASGRQITSLKRLFGVVSDR
CCEEEECCCCCEEEECCCHHHHHHHHHHHHHHHHCCEEEECCCCCHHHHHHHHHHHHHHH
LAYIAENGALVEYRGKTIGKTPMERKLALEIIEDVIEQPNCEVLVSGEHTAYIKPKSEEY
HHHHHCCCCEEEECCCCCCCCCHHHHHHHHHHHHHHCCCCCEEEECCCEEEEECCCCCHH
YYRMTKVVNYHTTLVDKFTDIDEDILKIAVCDMTGIKNSKEHFINKWSDKASVLVSGELY
HHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCCCCCHHHHHHHCCCCCEEEEECEEE
LDLMDTNVNKGRGIEQVQQYFGLKPEECMAFGDNYNDIAMLDNVYYSYVMEKAVDDVKKH
EEEEECCCCCCCCHHHHHHHHCCCHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHC
GRFITGWVEGTLREQFKEIR
CCEEEEHHHHHHHHHHHHCC
>Mature Secondary Structure
MVKFVASDLDGTLLLNGAQSVDESAVQYINKLVDNGVIFAPASGRQITSLKRLFGVVSDR
CCEEEECCCCCEEEECCCHHHHHHHHHHHHHHHHCCEEEECCCCCHHHHHHHHHHHHHHH
LAYIAENGALVEYRGKTIGKTPMERKLALEIIEDVIEQPNCEVLVSGEHTAYIKPKSEEY
HHHHHCCCCEEEECCCCCCCCCHHHHHHHHHHHHHHCCCCCEEEECCCEEEEECCCCCHH
YYRMTKVVNYHTTLVDKFTDIDEDILKIAVCDMTGIKNSKEHFINKWSDKASVLVSGELY
HHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCCCCCHHHHHHHCCCCCEEEEECEEE
LDLMDTNVNKGRGIEQVQQYFGLKPEECMAFGDNYNDIAMLDNVYYSYVMEKAVDDVKKH
EEEEECCCCCCCCHHHHHHHHCCCHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHC
GRFITGWVEGTLREQFKEIR
CCEEEEHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8905232; 9278503; 10493123 [H]