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The map label for this gene is gyrB [H]

Identifier: 238917147

GI number: 238917147

Start: 1235778

End: 1237694

Strand: Reverse

Name: gyrB [H]

Synonym: EUBELI_01219

Alternate gene names: 238917147

Gene position: 1237694-1235778 (Counterclockwise)

Preceding gene: 238917148

Following gene: 238917146

Centisome position: 57.72

GC content: 40.01

Gene sequence:

>1917_bases
ATGGCAGCTAATAAATATGATGCTAACAGTATATCAATACTGGAAGGTCTTGAAGCGGTAAGAATGAGACCGGGTATGTA
TATTGGAAGTGTTGGAACTAAGGGACTTAATCATCTTATATATGAGATTGCGGATAATTCAGTGGATGAGCATCTAGCAG
GTTTTTGTACACAGATAAATGTAACTCTTAATGATGATGGAACAGCAACTATCAAGGATAATGGACGAGGCATTCCTATA
GGAATACACCCTAAAGCCGGAATACCTGCGGTTGAGGTTGTATTTACAGTACTTCATGCAGGCGGTAAGTTCGGAGATGG
CGGATATAAGATATCAGGAGGACTTCATGGAGTTGGTGCGTCTGTTGTTAATGCACTTTCTGTATGGCTTGAGGTTGAGA
TAAGAGTTGACGGTGGAGTGTACAAGCAAAGATACGAGCGTGGAAAAGCAACAGCGCCGCTTGAAAAGATTGGTACATGC
AGAAAAAATGATACTGGTACAACTGTTACATTTCTTCCTGATGGAGAAATATTTGAAAAGACAAGATTCAAAGCAGACTC
GATAAAGAGCAGGCTTCATGAGACTGCATATCTTAATCCGGGGCTTACAATTGAGTTTGAAGATAAGAGAAAGGGCAGCG
AGGATAAGGAAACTTTCCATGAGCCTGACGGACTTAAGGCATATATCAAAGACCTTAACAATGGCAAAGAGACAGTATGC
GATATCGTCTACTTTAAGAAGAAGCAGGAAGATATTGAGGTTGAAGTAGCATTTCAGTTTGTTAATGAATTTGAGGAAAA
TATTCTTGGCTTCTGTAATAACATATACACTCAGGAGGGTGGAACGCATATTACAGGCTTTAAGACCAAGTTTACAATGA
TTATCAACCAGTATGCGAGAGAACTTGGCATCTTAAAGGATAAGGATGCGAATTTTACAGGTCTTGATGTAAGAAATGGT
ATGACTGCAGTTGTTGCAGTTAAGCATCCTGCGCCAAGATTTGAGGGACAGACCAAGACCAAGCTTGATAATCCGGATGC
TGGTACAGTTGTTGCAAATGTAACAGGTGACGAGGTACAGCTTTATTTCGACAGACATTTGGACGATCTTAACGCTGTAA
TTGCATGCGCTGAAAAATCTGCGAAGATAAGAAAAGCAGAAGAAAAGGCCAAGACAAATATGCTTACAAAGCAGAAGTTC
TCTATAGACAGCAACGGAAAGCTTGCTAATTGTGAGAGTAAGAATCCGGAGGAATGTGAGGTATTCATTGTAGAGGGAGA
TTCAGCCGGAGGTTCAGCAAAGACTGCAAGAAACAGAAGAACTCAGGCAATTCTTCCAATCAGAGGTAAGATTCTTAATG
TTGAAAAGGCAAGTATTGATAAGGTACTTGCCAATGCCGAGATTAAGACAATGATTAATTCATTCAACTGTGGATTCTTA
GAGGGCTACGGTAATGACTTTGATATTAGCAGATTAAGATATAATAAGATTATTATCATGACTGATGCCGATGTGGATGG
AGCACATATTGATACACTGCTTCTTACATTTTTCTACAGATTCATGCCTGAGCTTATTAATCAGGGACATGTATATATTG
CTACACCACCTCTTTACAAGGTTGTTCCTAAGAAGGGCGAGGGTGAATATCTATATGATGACAAGGCACTTGAGGATTAC
AGAAAGAAGCATACGTCAGGAACATTTACACTTCAGAGATACAAAGGTCTTGGAGAGATGGATCCTGACCAGCTATGGGA
GACAACTCTTAATCCTGAAACAAGAATTTTAAAGCAGGTTGAGATTGAGGACGCAAGGCTTGCAACTGAGGTTACAGCAA
TGCTTATGGGTACAGATGTACCACCTAGAAGAGAATTTATATATACGCATGCTAATGAAGCTGAGATTGATGCGTAA

Upstream 100 bases:

>100_bases
CGTGGAAAAAATTAAGTATCAGAGATGGTTACACATTAGATAGCTGCTTTGAAGATATTTTTAACACATATTTTGGAAAG
TAAGAGGAGCGTAATTAATA

Downstream 100 bases:

>100_bases
AAGCTAAAGGAGTAGAAAATGGCTGAGAAGATTATAAGGACTGAGTATTCTGAGGTTATGCAGAAGTCCTATATAGATTA
TTCCATGAGTGTTATTACAG

Product: DNA gyrase subunit B

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 638; Mature: 637

Protein sequence:

>638_residues
MAANKYDANSISILEGLEAVRMRPGMYIGSVGTKGLNHLIYEIADNSVDEHLAGFCTQINVTLNDDGTATIKDNGRGIPI
GIHPKAGIPAVEVVFTVLHAGGKFGDGGYKISGGLHGVGASVVNALSVWLEVEIRVDGGVYKQRYERGKATAPLEKIGTC
RKNDTGTTVTFLPDGEIFEKTRFKADSIKSRLHETAYLNPGLTIEFEDKRKGSEDKETFHEPDGLKAYIKDLNNGKETVC
DIVYFKKKQEDIEVEVAFQFVNEFEENILGFCNNIYTQEGGTHITGFKTKFTMIINQYARELGILKDKDANFTGLDVRNG
MTAVVAVKHPAPRFEGQTKTKLDNPDAGTVVANVTGDEVQLYFDRHLDDLNAVIACAEKSAKIRKAEEKAKTNMLTKQKF
SIDSNGKLANCESKNPEECEVFIVEGDSAGGSAKTARNRRTQAILPIRGKILNVEKASIDKVLANAEIKTMINSFNCGFL
EGYGNDFDISRLRYNKIIIMTDADVDGAHIDTLLLTFFYRFMPELINQGHVYIATPPLYKVVPKKGEGEYLYDDKALEDY
RKKHTSGTFTLQRYKGLGEMDPDQLWETTLNPETRILKQVEIEDARLATEVTAMLMGTDVPPRREFIYTHANEAEIDA

Sequences:

>Translated_638_residues
MAANKYDANSISILEGLEAVRMRPGMYIGSVGTKGLNHLIYEIADNSVDEHLAGFCTQINVTLNDDGTATIKDNGRGIPI
GIHPKAGIPAVEVVFTVLHAGGKFGDGGYKISGGLHGVGASVVNALSVWLEVEIRVDGGVYKQRYERGKATAPLEKIGTC
RKNDTGTTVTFLPDGEIFEKTRFKADSIKSRLHETAYLNPGLTIEFEDKRKGSEDKETFHEPDGLKAYIKDLNNGKETVC
DIVYFKKKQEDIEVEVAFQFVNEFEENILGFCNNIYTQEGGTHITGFKTKFTMIINQYARELGILKDKDANFTGLDVRNG
MTAVVAVKHPAPRFEGQTKTKLDNPDAGTVVANVTGDEVQLYFDRHLDDLNAVIACAEKSAKIRKAEEKAKTNMLTKQKF
SIDSNGKLANCESKNPEECEVFIVEGDSAGGSAKTARNRRTQAILPIRGKILNVEKASIDKVLANAEIKTMINSFNCGFL
EGYGNDFDISRLRYNKIIIMTDADVDGAHIDTLLLTFFYRFMPELINQGHVYIATPPLYKVVPKKGEGEYLYDDKALEDY
RKKHTSGTFTLQRYKGLGEMDPDQLWETTLNPETRILKQVEIEDARLATEVTAMLMGTDVPPRREFIYTHANEAEIDA
>Mature_637_residues
AANKYDANSISILEGLEAVRMRPGMYIGSVGTKGLNHLIYEIADNSVDEHLAGFCTQINVTLNDDGTATIKDNGRGIPIG
IHPKAGIPAVEVVFTVLHAGGKFGDGGYKISGGLHGVGASVVNALSVWLEVEIRVDGGVYKQRYERGKATAPLEKIGTCR
KNDTGTTVTFLPDGEIFEKTRFKADSIKSRLHETAYLNPGLTIEFEDKRKGSEDKETFHEPDGLKAYIKDLNNGKETVCD
IVYFKKKQEDIEVEVAFQFVNEFEENILGFCNNIYTQEGGTHITGFKTKFTMIINQYARELGILKDKDANFTGLDVRNGM
TAVVAVKHPAPRFEGQTKTKLDNPDAGTVVANVTGDEVQLYFDRHLDDLNAVIACAEKSAKIRKAEEKAKTNMLTKQKFS
IDSNGKLANCESKNPEECEVFIVEGDSAGGSAKTARNRRTQAILPIRGKILNVEKASIDKVLANAEIKTMINSFNCGFLE
GYGNDFDISRLRYNKIIIMTDADVDGAHIDTLLLTFFYRFMPELINQGHVYIATPPLYKVVPKKGEGEYLYDDKALEDYR
KKHTSGTFTLQRYKGLGEMDPDQLWETTLNPETRILKQVEIEDARLATEVTAMLMGTDVPPRREFIYTHANEAEIDA

Specific function: DNA gyrase negatively supercoils closed circular double- stranded DNA in an ATP-dependent manner and also catalyzes the interconversion of other topological isomers of double-stranded DNA rings, including catenanes and knotted rings [H]

COG id: COG0187

COG function: function code L; Type IIA topoisomerase (DNA gyrase/topo II, topoisomerase IV), B subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Toprim domain [H]

Homologues:

Organism=Homo sapiens, GI19913408, Length=621, Percent_Identity=24.7987117552335, Blast_Score=132, Evalue=7e-31,
Organism=Homo sapiens, GI19913406, Length=618, Percent_Identity=26.537216828479, Blast_Score=129, Evalue=1e-29,
Organism=Escherichia coli, GI48994957, Length=568, Percent_Identity=47.7112676056338, Blast_Score=500, Evalue=1e-143,
Organism=Escherichia coli, GI1789408, Length=642, Percent_Identity=38.1619937694704, Blast_Score=408, Evalue=1e-115,
Organism=Caenorhabditis elegans, GI17535065, Length=618, Percent_Identity=24.9190938511327, Blast_Score=138, Evalue=7e-33,
Organism=Caenorhabditis elegans, GI212645845, Length=549, Percent_Identity=25.8652094717668, Blast_Score=127, Evalue=1e-29,
Organism=Caenorhabditis elegans, GI212645657, Length=172, Percent_Identity=29.6511627906977, Blast_Score=87, Evalue=2e-17,
Organism=Saccharomyces cerevisiae, GI6324241, Length=635, Percent_Identity=26.1417322834646, Blast_Score=130, Evalue=5e-31,
Organism=Drosophila melanogaster, GI17136538, Length=609, Percent_Identity=25.4515599343186, Blast_Score=138, Evalue=1e-32,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR011557
- InterPro:   IPR020568
- InterPro:   IPR014721
- InterPro:   IPR001241
- InterPro:   IPR013759
- InterPro:   IPR002288
- InterPro:   IPR013506
- InterPro:   IPR013760
- InterPro:   IPR018522
- InterPro:   IPR006171 [H]

Pfam domain/function: PF00204 DNA_gyraseB; PF00986 DNA_gyraseB_C; PF02518 HATPase_c; PF01751 Toprim [H]

EC number: =5.99.1.3 [H]

Molecular weight: Translated: 70823; Mature: 70692

Theoretical pI: Translated: 5.58; Mature: 5.58

Prosite motif: PS00177 TOPOISOMERASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAANKYDANSISILEGLEAVRMRPGMYIGSVGTKGLNHLIYEIADNSVDEHLAGFCTQIN
CCCCCCCCCCHHHHHHHHHHHCCCCEEEECCCCHHHHHHHHHHHCCCHHHHHHCEEEEEE
VTLNDDGTATIKDNGRGIPIGIHPKAGIPAVEVVFTVLHAGGKFGDGGYKISGGLHGVGA
EEECCCCEEEECCCCCEEEEEECCCCCCCHHHHHHHHHHCCCCCCCCCEEECCCCCCHHH
SVVNALSVWLEVEIRVDGGVYKQRYERGKATAPLEKIGTCRKNDTGTTVTFLPDGEIFEK
HHHHHHEEEEEEEEEECCCHHHHHHHCCCCCCCHHHHCCCCCCCCCCEEEECCCCCHHHH
TRFKADSIKSRLHETAYLNPGLTIEFEDKRKGSEDKETFHEPDGLKAYIKDLNNGKETVC
HHCCHHHHHHHHHHHHCCCCCCEEEECCCCCCCCHHHHHCCCCCHHHHHHHCCCCHHHHH
DIVYFKKKQEDIEVEVAFQFVNEFEENILGFCNNIYTQEGGTHITGFKTKFTMIINQYAR
HHHHEECCCCCCEEEHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCHHHHHHHHHHHH
ELGILKDKDANFTGLDVRNGMTAVVAVKHPAPRFEGQTKTKLDNPDAGTVVANVTGDEVQ
HHCCEECCCCCEEEEEECCCCEEEEEEECCCCCCCCCCCCCCCCCCCCEEEEEECCCEEE
LYFDRHLDDLNAVIACAEKSAKIRKAEEKAKTNMLTKQKFSIDSNGKLANCESKNPEECE
EEEHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCCCEECCCCCCCCCEE
VFIVEGDSAGGSAKTARNRRTQAILPIRGKILNVEKASIDKVLANAEIKTMINSFNCGFL
EEEEECCCCCCCCHHHHCCCCEEEEEECCEEEECCHHHHHHHHHCCHHHHHHHCCCCCEE
EGYGNDFDISRLRYNKIIIMTDADVDGAHIDTLLLTFFYRFMPELINQGHVYIATPPLYK
ECCCCCCCCEEEEEEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCEE
VVPKKGEGEYLYDDKALEDYRKKHTSGTFTLQRYKGLGEMDPDQLWETTLNPETRILKQV
ECCCCCCCCEEECCHHHHHHHHHCCCCCEEEHHHCCCCCCCHHHHHHCCCCCHHHHHHHH
EIEDARLATEVTAMLMGTDVPPRREFIYTHANEAEIDA
CHHHHHHHHHHHHHHHCCCCCCCCCEEEEECCCCCCCC
>Mature Secondary Structure 
AANKYDANSISILEGLEAVRMRPGMYIGSVGTKGLNHLIYEIADNSVDEHLAGFCTQIN
CCCCCCCCCHHHHHHHHHHHCCCCEEEECCCCHHHHHHHHHHHCCCHHHHHHCEEEEEE
VTLNDDGTATIKDNGRGIPIGIHPKAGIPAVEVVFTVLHAGGKFGDGGYKISGGLHGVGA
EEECCCCEEEECCCCCEEEEEECCCCCCCHHHHHHHHHHCCCCCCCCCEEECCCCCCHHH
SVVNALSVWLEVEIRVDGGVYKQRYERGKATAPLEKIGTCRKNDTGTTVTFLPDGEIFEK
HHHHHHEEEEEEEEEECCCHHHHHHHCCCCCCCHHHHCCCCCCCCCCEEEECCCCCHHHH
TRFKADSIKSRLHETAYLNPGLTIEFEDKRKGSEDKETFHEPDGLKAYIKDLNNGKETVC
HHCCHHHHHHHHHHHHCCCCCCEEEECCCCCCCCHHHHHCCCCCHHHHHHHCCCCHHHHH
DIVYFKKKQEDIEVEVAFQFVNEFEENILGFCNNIYTQEGGTHITGFKTKFTMIINQYAR
HHHHEECCCCCCEEEHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCHHHHHHHHHHHH
ELGILKDKDANFTGLDVRNGMTAVVAVKHPAPRFEGQTKTKLDNPDAGTVVANVTGDEVQ
HHCCEECCCCCEEEEEECCCCEEEEEEECCCCCCCCCCCCCCCCCCCCEEEEEECCCEEE
LYFDRHLDDLNAVIACAEKSAKIRKAEEKAKTNMLTKQKFSIDSNGKLANCESKNPEECE
EEEHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCCCEECCCCCCCCCEE
VFIVEGDSAGGSAKTARNRRTQAILPIRGKILNVEKASIDKVLANAEIKTMINSFNCGFL
EEEEECCCCCCCCHHHHCCCCEEEEEECCEEEECCHHHHHHHHHCCHHHHHHHCCCCCEE
EGYGNDFDISRLRYNKIIIMTDADVDGAHIDTLLLTFFYRFMPELINQGHVYIATPPLYK
ECCCCCCCCEEEEEEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCEE
VVPKKGEGEYLYDDKALEDYRKKHTSGTFTLQRYKGLGEMDPDQLWETTLNPETRILKQV
ECCCCCCCCEEECCHHHHHHHHHCCCCCEEEHHHCCCCCCCHHHHHHCCCCCHHHHHHHH
EIEDARLATEVTAMLMGTDVPPRREFIYTHANEAEIDA
CHHHHHHHHHHHHHHHCCCCCCCCCEEEEECCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11466286 [H]