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The map label for this gene is smpB [H]

Identifier: 238917113

GI number: 238917113

Start: 1195469

End: 1195963

Strand: Reverse

Name: smpB [H]

Synonym: EUBELI_01184

Alternate gene names: 238917113

Gene position: 1195963-1195469 (Counterclockwise)

Preceding gene: 238917114

Following gene: 238917112

Centisome position: 55.78

GC content: 35.96

Gene sequence:

>495_bases
ATGGAGACTGACATGGCATCAAAAACAAGCGCATCTGGAAACAGACTTATAGCGAACAATAAGAAAGCTTATTTTGATTA
TTTTATAGAAGAGAAATACGAGGCGGGCATGGTACTTCACGGCACTGAGGTTAAATCAATCAGAGCAGGTAAGTGCTCAA
TTAAGGAATCTTATATCAAGATTGAGAATGGCGAAGTGTATGTATTAGGTATGAATATTACACCATATGAGAAGGGAAAC
ATATTCAATAAAGATCCTTTAAGACCTAAGAAGCTGCTTCTTCACAAGAATCAGATTAATAAGCTTACTGGTGAACTTAC
AATTAAAGGTTACACACTTGTTCCTCTTCAGGTATATCTGAAAGAGGGAAAGGTGAAGATGGAAATTGGTCTTGGAAAAG
GTAAGAAGAACTTTGACAAGAGAGATTCTATTGCAAAGAAAGACCAGATAAGAGATGCACAGAAGGAATTCAAGAATAAG
AATCTCAGATTTTAG

Upstream 100 bases:

>100_bases
TGGGAACAGAAGTTATAAGCTCGGACAGAAGGTTAAGGTTGTGGTTATAGGCACCGATAAGATACTAAGAACAATTGATT
TTGCATTTGCATAGAATAGA

Downstream 100 bases:

>100_bases
AAATATTTTATCAGTTTTGAATAACAAGTTCATTGACTTGGCATTATGCCAATGTTAATATAAATAAGCAGATAAAACTG
CTTACACACGGGCTAGTAAA

Product: SsrA-binding protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 164; Mature: 164

Protein sequence:

>164_residues
METDMASKTSASGNRLIANNKKAYFDYFIEEKYEAGMVLHGTEVKSIRAGKCSIKESYIKIENGEVYVLGMNITPYEKGN
IFNKDPLRPKKLLLHKNQINKLTGELTIKGYTLVPLQVYLKEGKVKMEIGLGKGKKNFDKRDSIAKKDQIRDAQKEFKNK
NLRF

Sequences:

>Translated_164_residues
METDMASKTSASGNRLIANNKKAYFDYFIEEKYEAGMVLHGTEVKSIRAGKCSIKESYIKIENGEVYVLGMNITPYEKGN
IFNKDPLRPKKLLLHKNQINKLTGELTIKGYTLVPLQVYLKEGKVKMEIGLGKGKKNFDKRDSIAKKDQIRDAQKEFKNK
NLRF
>Mature_164_residues
METDMASKTSASGNRLIANNKKAYFDYFIEEKYEAGMVLHGTEVKSIRAGKCSIKESYIKIENGEVYVLGMNITPYEKGN
IFNKDPLRPKKLLLHKNQINKLTGELTIKGYTLVPLQVYLKEGKVKMEIGLGKGKKNFDKRDSIAKKDQIRDAQKEFKNK
NLRF

Specific function: Binds specifically to the ssrA RNA (tmRNA) and is required for stable association of ssrA with ribosomes [H]

COG id: COG0691

COG function: function code O; tmRNA-binding protein

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the smpB family [H]

Homologues:

Organism=Escherichia coli, GI1788973, Length=155, Percent_Identity=41.9354838709677, Blast_Score=128, Evalue=2e-31,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000037
- InterPro:   IPR020081
- ProDom:   PD004488 [H]

Pfam domain/function: PF01668 SmpB [H]

EC number: NA

Molecular weight: Translated: 18769; Mature: 18769

Theoretical pI: Translated: 10.27; Mature: 10.27

Prosite motif: PS01317 SSRP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
METDMASKTSASGNRLIANNKKAYFDYFIEEKYEAGMVLHGTEVKSIRAGKCSIKESYIK
CCCCCCCCCCCCCCEEEECCCCEEEHHEEHHHCCCCEEEECCCCCEEECCCCCEEEEEEE
IENGEVYVLGMNITPYEKGNIFNKDPLRPKKLLLHKNQINKLTGELTIKGYTLVPLQVYL
EECCEEEEEECCCCCCCCCCCCCCCCCCHHHHHEEHHHHHHCCCEEEEECEEEEEEEEEE
KEGKVKMEIGLGKGKKNFDKRDSIAKKDQIRDAQKEFKNKNLRF
ECCCEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure
METDMASKTSASGNRLIANNKKAYFDYFIEEKYEAGMVLHGTEVKSIRAGKCSIKESYIK
CCCCCCCCCCCCCCEEEECCCCEEEHHEEHHHCCCCEEEECCCCCEEECCCCCEEEEEEE
IENGEVYVLGMNITPYEKGNIFNKDPLRPKKLLLHKNQINKLTGELTIKGYTLVPLQVYL
EECCEEEEEECCCCCCCCCCCCCCCCCCHHHHHEEHHHHHHCCCEEEEECEEEEEEEEEE
KEGKVKMEIGLGKGKKNFDKRDSIAKKDQIRDAQKEFKNKNLRF
ECCCEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA