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The map label for this gene is pckA

Identifier: 238917099

GI number: 238917099

Start: 1182141

End: 1183745

Strand: Reverse

Name: pckA

Synonym: EUBELI_01169

Alternate gene names: 238917099

Gene position: 1183745-1182141 (Counterclockwise)

Preceding gene: 238917100

Following gene: 238917098

Centisome position: 55.21

GC content: 40.62

Gene sequence:

>1605_bases
ATGGCAAAAATCGATTTAAGCAAGTATGGCATCACAGGTGCTACAGAGATCGTCTACAATCCTTCTTATGAATTATTATT
CGAGGAAGAGACTAAGTCTACTAACGAAGGTTATGAAGTTGGTAAGGAAAGCGAACTTGGAGCTGTTGATGTTATGACAG
GTATCTATACAGGTCGTTCTCCTAAAGACAAGTACATTGTTGTTGATGAGAACTCTAAGGACACTGTATGGTGGACATCA
GATGAGTATAAGAACGATAACCACCCAATGTCAGAAGATGTATGGGCTAAGGTTAAGGACATCGCTAAGGCAGAGCTTTC
TAATAAGAAACTTTTCGTAGTTGATGCATTTTGTGGAGCTAACAAGGACACAAGAATGGCAGTTCGTTTCATCGTTGAAG
TTGCTTGGCAGGCACATTTTGTAACTAATATGTTCATTAAGCCAACAGCAGAAGAACTTGAGAACTTCGAGCCAGATTTC
GTTGTATATAATGCTTCTAAGGCTAAGGTTGAGAACTATGCTGAATTAGGCCTTAACTCAGAGACATGTGTTGCATTCAA
CATTACAAGCCATGAGCAGGTAATCATTAATACATGGTATGGTGGAGAGATGAAGAAGGGTATGTTCTCTATGATGAACT
ACTACCTTCCATTAAAGGGTATTGCTTCAATGCACTGCTCAGCTAACACAGATAAGAACGGAGAGAATACAGCAATCTTC
TTTGGTCTTTCAGGAACAGGTAAGACAACACTTTCAACAGATCCTAAGAGACTCCTTATCGGTGATGATGAGCATGGTTG
GGATGACAATGGCGTATTCAACTTCGAAGGTGGATGCTACGCTAAGGTTATCAACCTTGATAAGGACTCAGAGCCAGATA
TCTACAATGCAATTAAGAGAAACGCTCTTCTTGAGAACGTTACACTTGATGAGAATGGAAAGATTGATTTTGCAGATAAG
AGCGTAACAGAGAATACTCGTGTATCTTACCCAATCAATCATATTGAGAATATAGTAAGACCAATTTCTTCAGCTCCAGC
AGCTAAGAATGTAATCTTCCTTTCAGCAGATGCTTTCGGAGTACTTCCTCCAGTATCTATTCTTACACCAGAGCAGACAC
AGTACTACTTCCTTTCAGGATTTACTGCTAAGCTTGCTGGTACAGAGAGAGGAATCACAGAGCCTACACCTACATTCTCA
GCTTGCTTCGGACAGGCATTCCTTGAGCTTCATCCTACAAAGTATGCAGCAGAGCTTGTTAAGAAGATGGAAAAGAGTGG
AGCTAAGGCTTACTTAGTTAACACAGGATGGAATGGAACAGGTAAGAGAATCTCTATTAAGGATACTCGTGGTATCATTG
ATGCTATCCTTTCAGGTGCTATTAATACAGCTCCAACAAAGAAGATTCCTATGTTCAACTTTGAAGTTCCTACAGAGCTT
CCTGGAGTAGATCCAGCAATCCTTGATCCAAGAGATACATATGCTGATGCTTCTGAGTGGGAGACTAAGGCTAAGGATCT
TGCTGCAAGATTCAATAAGAACTTTGTTAAGTACACAGGTAACGAAGCTGGTAAGGCTTTAGTTGCTGCTGGTCCACAGC
TTTAA

Upstream 100 bases:

>100_bases
TTTAACAATTTTTTATTGATTTTAATTAGGGCATGGTATAGAATTAAAAAGAATTTTGTAATAATTTACAAGAAATTATA
AGAAAAGAGGTTTATGTAAG

Downstream 100 bases:

>100_bases
TTTTCAATCATTACAGAGTTTGAATTATGATTATATACCTGCGTCTGAATTGAATTCATTCGCAGGTATATTTGAATTTT
TTTGACATATAATATTGTGT

Product: phosphoenolpyruvate carboxykinase

Products: NA

Alternate protein names: PEP carboxykinase; PEPCK; Phosphoenolpyruvate carboxylase

Number of amino acids: Translated: 534; Mature: 533

Protein sequence:

>534_residues
MAKIDLSKYGITGATEIVYNPSYELLFEEETKSTNEGYEVGKESELGAVDVMTGIYTGRSPKDKYIVVDENSKDTVWWTS
DEYKNDNHPMSEDVWAKVKDIAKAELSNKKLFVVDAFCGANKDTRMAVRFIVEVAWQAHFVTNMFIKPTAEELENFEPDF
VVYNASKAKVENYAELGLNSETCVAFNITSHEQVIINTWYGGEMKKGMFSMMNYYLPLKGIASMHCSANTDKNGENTAIF
FGLSGTGKTTLSTDPKRLLIGDDEHGWDDNGVFNFEGGCYAKVINLDKDSEPDIYNAIKRNALLENVTLDENGKIDFADK
SVTENTRVSYPINHIENIVRPISSAPAAKNVIFLSADAFGVLPPVSILTPEQTQYYFLSGFTAKLAGTERGITEPTPTFS
ACFGQAFLELHPTKYAAELVKKMEKSGAKAYLVNTGWNGTGKRISIKDTRGIIDAILSGAINTAPTKKIPMFNFEVPTEL
PGVDPAILDPRDTYADASEWETKAKDLAARFNKNFVKYTGNEAGKALVAAGPQL

Sequences:

>Translated_534_residues
MAKIDLSKYGITGATEIVYNPSYELLFEEETKSTNEGYEVGKESELGAVDVMTGIYTGRSPKDKYIVVDENSKDTVWWTS
DEYKNDNHPMSEDVWAKVKDIAKAELSNKKLFVVDAFCGANKDTRMAVRFIVEVAWQAHFVTNMFIKPTAEELENFEPDF
VVYNASKAKVENYAELGLNSETCVAFNITSHEQVIINTWYGGEMKKGMFSMMNYYLPLKGIASMHCSANTDKNGENTAIF
FGLSGTGKTTLSTDPKRLLIGDDEHGWDDNGVFNFEGGCYAKVINLDKDSEPDIYNAIKRNALLENVTLDENGKIDFADK
SVTENTRVSYPINHIENIVRPISSAPAAKNVIFLSADAFGVLPPVSILTPEQTQYYFLSGFTAKLAGTERGITEPTPTFS
ACFGQAFLELHPTKYAAELVKKMEKSGAKAYLVNTGWNGTGKRISIKDTRGIIDAILSGAINTAPTKKIPMFNFEVPTEL
PGVDPAILDPRDTYADASEWETKAKDLAARFNKNFVKYTGNEAGKALVAAGPQL
>Mature_533_residues
AKIDLSKYGITGATEIVYNPSYELLFEEETKSTNEGYEVGKESELGAVDVMTGIYTGRSPKDKYIVVDENSKDTVWWTSD
EYKNDNHPMSEDVWAKVKDIAKAELSNKKLFVVDAFCGANKDTRMAVRFIVEVAWQAHFVTNMFIKPTAEELENFEPDFV
VYNASKAKVENYAELGLNSETCVAFNITSHEQVIINTWYGGEMKKGMFSMMNYYLPLKGIASMHCSANTDKNGENTAIFF
GLSGTGKTTLSTDPKRLLIGDDEHGWDDNGVFNFEGGCYAKVINLDKDSEPDIYNAIKRNALLENVTLDENGKIDFADKS
VTENTRVSYPINHIENIVRPISSAPAAKNVIFLSADAFGVLPPVSILTPEQTQYYFLSGFTAKLAGTERGITEPTPTFSA
CFGQAFLELHPTKYAAELVKKMEKSGAKAYLVNTGWNGTGKRISIKDTRGIIDAILSGAINTAPTKKIPMFNFEVPTELP
GVDPAILDPRDTYADASEWETKAKDLAARFNKNFVKYTGNEAGKALVAAGPQL

Specific function: Rate-limiting gluconeogenic enzyme. [C]

COG id: COG1866

COG function: function code C; Phosphoenolpyruvate carboxykinase (ATP)

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the phosphoenolpyruvate carboxykinase [ATP] family

Homologues:

Organism=Escherichia coli, GI1789807, Length=531, Percent_Identity=68.1732580037665, Blast_Score=751, Evalue=0.0,
Organism=Saccharomyces cerevisiae, GI6322950, Length=501, Percent_Identity=46.3073852295409, Blast_Score=449, Evalue=1e-127,

Paralogues:

None

Copy number: 20 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): PCKA_EUBE2 (C4Z0Q6)

Other databases:

- EMBL:   CP001104
- RefSeq:   YP_002930616.1
- GeneID:   7957713
- GenomeReviews:   CP001104_GR
- KEGG:   eel:EUBELI_01169
- OMA:   MFIRPTE
- ProtClustDB:   PRK09344
- GO:   GO:0005737
- GO:   GO:0006094
- HAMAP:   MF_00453
- InterPro:   IPR001272
- InterPro:   IPR013035
- InterPro:   IPR008210
- InterPro:   IPR015994
- Gene3D:   G3DSA:3.90.228.20
- Gene3D:   G3DSA:3.40.449.10
- PIRSF:   PIRSF006294
- TIGRFAMs:   TIGR00224

Pfam domain/function: PF01293 PEPCK_ATP; SSF68923 PEP_carboxykinase_N

EC number: =4.1.1.49

Molecular weight: Translated: 58997; Mature: 58866

Theoretical pI: Translated: 4.78; Mature: 4.78

Prosite motif: PS00532 PEPCK_ATP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKIDLSKYGITGATEIVYNPSYELLFEEETKSTNEGYEVGKESELGAVDVMTGIYTGRS
CCEEECHHCCCCCCEEEEECCCEEEEEECCCCCCCCCCCCCCCCCCCCEEHHHHHCCCCC
PKDKYIVVDENSKDTVWWTSDEYKNDNHPMSEDVWAKVKDIAKAELSNKKLFVVDAFCGA
CCCCEEEEECCCCCCEEECCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCEEEEEEECCCC
NKDTRMAVRFIVEVAWQAHFVTNMFIKPTAEELENFEPDFVVYNASKAKVENYAELGLNS
CCCHHHHHHHHHHHHHHHHHEEEEEECCCHHHHHCCCCCEEEEECCHHHHHHHHHHCCCC
ETCVAFNITSHEQVIINTWYGGEMKKGMFSMMNYYLPLKGIASMHCSANTDKNGENTAIF
CCEEEEEECCCCEEEEEEECCCCHHHHHHHHHHHHCCCCHHHHEECCCCCCCCCCCEEEE
FGLSGTGKTTLSTDPKRLLIGDDEHGWDDNGVFNFEGGCYAKVINLDKDSEPDIYNAIKR
EEECCCCCCEECCCCCEEEECCCCCCCCCCCEEEECCCEEEEEEECCCCCCCHHHHHHHH
NALLENVTLDENGKIDFADKSVTENTRVSYPINHIENIVRPISSAPAAKNVIFLSADAFG
HHHHHCCEECCCCCEEECCCCCCCCCEEECCHHHHHHHHHHHCCCCCCCCEEEEECCCCC
VLPPVSILTPEQTQYYFLSGFTAKLAGTERGITEPTPTFSACFGQAFLELHPTKYAAELV
CCCCCEEECCCCCEEEEEECCEEEECCCCCCCCCCCCHHHHHHHHHHHEECCHHHHHHHH
KKMEKSGAKAYLVNTGWNGTGKRISIKDTRGIIDAILSGAINTAPTKKIPMFNFEVPTEL
HHHHHCCCEEEEEECCCCCCCCEEEEHHHHHHHHHHHHCCCCCCCCCCCCEEECCCCCCC
PGVDPAILDPRDTYADASEWETKAKDLAARFNKNFVKYTGNEAGKALVAAGPQL
CCCCCCEECCCCCCCCCHHHHHHHHHHHHHHCCCEEEECCCCCCCEEEECCCCC
>Mature Secondary Structure 
AKIDLSKYGITGATEIVYNPSYELLFEEETKSTNEGYEVGKESELGAVDVMTGIYTGRS
CEEECHHCCCCCCEEEEECCCEEEEEECCCCCCCCCCCCCCCCCCCCEEHHHHHCCCCC
PKDKYIVVDENSKDTVWWTSDEYKNDNHPMSEDVWAKVKDIAKAELSNKKLFVVDAFCGA
CCCCEEEEECCCCCCEEECCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCEEEEEEECCCC
NKDTRMAVRFIVEVAWQAHFVTNMFIKPTAEELENFEPDFVVYNASKAKVENYAELGLNS
CCCHHHHHHHHHHHHHHHHHEEEEEECCCHHHHHCCCCCEEEEECCHHHHHHHHHHCCCC
ETCVAFNITSHEQVIINTWYGGEMKKGMFSMMNYYLPLKGIASMHCSANTDKNGENTAIF
CCEEEEEECCCCEEEEEEECCCCHHHHHHHHHHHHCCCCHHHHEECCCCCCCCCCCEEEE
FGLSGTGKTTLSTDPKRLLIGDDEHGWDDNGVFNFEGGCYAKVINLDKDSEPDIYNAIKR
EEECCCCCCEECCCCCEEEECCCCCCCCCCCEEEECCCEEEEEEECCCCCCCHHHHHHHH
NALLENVTLDENGKIDFADKSVTENTRVSYPINHIENIVRPISSAPAAKNVIFLSADAFG
HHHHHCCEECCCCCEEECCCCCCCCCEEECCHHHHHHHHHHHCCCCCCCCEEEEECCCCC
VLPPVSILTPEQTQYYFLSGFTAKLAGTERGITEPTPTFSACFGQAFLELHPTKYAAELV
CCCCCEEECCCCCEEEEEECCEEEECCCCCCCCCCCCHHHHHHHHHHHEECCHHHHHHHH
KKMEKSGAKAYLVNTGWNGTGKRISIKDTRGIIDAILSGAINTAPTKKIPMFNFEVPTEL
HHHHHCCCEEEEEECCCCCCCCEEEEHHHHHHHHHHHHCCCCCCCCCCCCEEECCCCCCC
PGVDPAILDPRDTYADASEWETKAKDLAARFNKNFVKYTGNEAGKALVAAGPQL
CCCCCCEECCCCCCCCCHHHHHHHHHHHHHHCCCEEEECCCCCCCEEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA