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The map label for this gene is iscS [H]

Identifier: 238917091

GI number: 238917091

Start: 1173125

End: 1174282

Strand: Reverse

Name: iscS [H]

Synonym: EUBELI_01161

Alternate gene names: 238917091

Gene position: 1174282-1173125 (Counterclockwise)

Preceding gene: 238917092

Following gene: 238917090

Centisome position: 54.77

GC content: 37.82

Gene sequence:

>1158_bases
ATGGAAGCATATCTTGATAATTCTGCTACAACGAAATGTGCAGCAGAGGTAGTAGAAACAGCAGTAAAGGTTATGTCAGA
AGATTATGGTAATCCATCATCAAAGCATATGAAGGGCGTTGATGCAGAGAAATATATCCGTGAAGCTAAAGAGGTAATAG
CAAAGACTCTTAAGTGTCAGGATAAGGAGATACTCTTTACTTCTGGTGGAACAGAATCTAATAACATGGCAATAATCGGA
ACAGCAATGGCTAATAAAAGAAAAGGAATGCACTGCATAGTATCATCTGTAGAACATTCATCAGTTAAAGAGCCATTCAA
TTTCCTTGAAAAAGAAGGCTTCAGAATAACATATCTTCCAGTTGATAAAGACGGAATAGTTGATATAGAGGCTCTAAAGG
ATGCACTTGATGATGAGACAATACTTGTTTCAGTAATGTATGTTAATAATGAAATTGGTGCAGTTGAACCGATTGAAGAA
ATCGGACATATAATTAAAGATTATAATCCTGATATTGTATATCATGTTGATGCGATTCAGGCTTATGGTAAATATAAGAT
ATATCCTAAGAAGCTTGGAATTGATCTGTTATCAGTCAGTGGACATAAGATTCACGGACCTAAGGGGAGCGGATTCTTAT
TCATTAATGATAAGACAAGAATCAAGCCTATTATATATGGTGGAGGACAGCAGAAGGGCATGCGTTCAGGAACTGAAAAT
GTTCCGGCTATAGCAGGACTTGCAACTGCCGTAAAGCTTATATATAATAATGACTTTGAAAGCAAGATAGCACATTTGTA
TGAGTTAAAGGATTACTTCATAGATGAGCTTGAAAAGCTTCCTGATGTGCAGGTAAACAGCAAGAAGGGTACGGATTCAG
CACCACAGATTGTAAGTGCAAGCTTTAAGGGAGTAAGGGCAGAAGTGCTTCTTCATTCACTTGAAGATAAAGGAATCTAT
GTATCATCAGGTTCAGCATGCTCATCTAACAAGCCGGGACTGAGCAACACACTTGTTGCAATCGGACTTGATAAGGAGCT
GCTTGATTCTACACTTCGGTTCAGCTTCTGCTATGAGACAACAAAGGAAGAACTTGAATACACAATAGAAACACTTAAAC
AGCTGCTTCCAATGCTTAGGAAGTACACAAGACGATAA

Upstream 100 bases:

>100_bases
ATCACACTAGGTAAGAGAATATTAAGAACTGAGACAGCGGGAATGATGCTGTTGTCAGTGCTTATGTATAATATGGAAGA
ATAAGCGGAAAAGAGGCAAT

Downstream 100 bases:

>100_bases
TTGTGTGAAAATAGATTTTCACACACAAGATTTGTGCTTTGCACAAATCTTGATATGCAAAGGAAAGGCGGAAAACGATG
TATAAAGCATTTTTGATAAA

Product: cysteine desulfurase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 385; Mature: 385

Protein sequence:

>385_residues
MEAYLDNSATTKCAAEVVETAVKVMSEDYGNPSSKHMKGVDAEKYIREAKEVIAKTLKCQDKEILFTSGGTESNNMAIIG
TAMANKRKGMHCIVSSVEHSSVKEPFNFLEKEGFRITYLPVDKDGIVDIEALKDALDDETILVSVMYVNNEIGAVEPIEE
IGHIIKDYNPDIVYHVDAIQAYGKYKIYPKKLGIDLLSVSGHKIHGPKGSGFLFINDKTRIKPIIYGGGQQKGMRSGTEN
VPAIAGLATAVKLIYNNDFESKIAHLYELKDYFIDELEKLPDVQVNSKKGTDSAPQIVSASFKGVRAEVLLHSLEDKGIY
VSSGSACSSNKPGLSNTLVAIGLDKELLDSTLRFSFCYETTKEELEYTIETLKQLLPMLRKYTRR

Sequences:

>Translated_385_residues
MEAYLDNSATTKCAAEVVETAVKVMSEDYGNPSSKHMKGVDAEKYIREAKEVIAKTLKCQDKEILFTSGGTESNNMAIIG
TAMANKRKGMHCIVSSVEHSSVKEPFNFLEKEGFRITYLPVDKDGIVDIEALKDALDDETILVSVMYVNNEIGAVEPIEE
IGHIIKDYNPDIVYHVDAIQAYGKYKIYPKKLGIDLLSVSGHKIHGPKGSGFLFINDKTRIKPIIYGGGQQKGMRSGTEN
VPAIAGLATAVKLIYNNDFESKIAHLYELKDYFIDELEKLPDVQVNSKKGTDSAPQIVSASFKGVRAEVLLHSLEDKGIY
VSSGSACSSNKPGLSNTLVAIGLDKELLDSTLRFSFCYETTKEELEYTIETLKQLLPMLRKYTRR
>Mature_385_residues
MEAYLDNSATTKCAAEVVETAVKVMSEDYGNPSSKHMKGVDAEKYIREAKEVIAKTLKCQDKEILFTSGGTESNNMAIIG
TAMANKRKGMHCIVSSVEHSSVKEPFNFLEKEGFRITYLPVDKDGIVDIEALKDALDDETILVSVMYVNNEIGAVEPIEE
IGHIIKDYNPDIVYHVDAIQAYGKYKIYPKKLGIDLLSVSGHKIHGPKGSGFLFINDKTRIKPIIYGGGQQKGMRSGTEN
VPAIAGLATAVKLIYNNDFESKIAHLYELKDYFIDELEKLPDVQVNSKKGTDSAPQIVSASFKGVRAEVLLHSLEDKGIY
VSSGSACSSNKPGLSNTLVAIGLDKELLDSTLRFSFCYETTKEELEYTIETLKQLLPMLRKYTRR

Specific function: Catalyzes the removal of elemental sulfur from cysteine to produce alanine [H]

COG id: COG1104

COG function: function code E; Cysteine sulfinate desulfinase/cysteine desulfurase and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-V pyridoxal-phosphate-dependent aminotransferase family. NifS/IscS subfamily [H]

Homologues:

Organism=Homo sapiens, GI32307132, Length=384, Percent_Identity=39.5833333333333, Blast_Score=259, Evalue=3e-69,
Organism=Homo sapiens, GI156713448, Length=413, Percent_Identity=34.8668280871671, Blast_Score=218, Evalue=5e-57,
Organism=Escherichia coli, GI48994898, Length=382, Percent_Identity=41.6230366492147, Blast_Score=271, Evalue=4e-74,
Organism=Escherichia coli, GI1787970, Length=397, Percent_Identity=21.6624685138539, Blast_Score=99, Evalue=5e-22,
Organism=Escherichia coli, GI1789175, Length=395, Percent_Identity=24.5569620253165, Blast_Score=96, Evalue=4e-21,
Organism=Caenorhabditis elegans, GI25143064, Length=372, Percent_Identity=41.3978494623656, Blast_Score=264, Evalue=7e-71,
Organism=Caenorhabditis elegans, GI17533177, Length=319, Percent_Identity=33.8557993730408, Blast_Score=166, Evalue=2e-41,
Organism=Saccharomyces cerevisiae, GI6319831, Length=383, Percent_Identity=39.4255874673629, Blast_Score=262, Evalue=8e-71,
Organism=Drosophila melanogaster, GI20129463, Length=389, Percent_Identity=38.8174807197943, Blast_Score=255, Evalue=4e-68,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000192
- InterPro:   IPR020578
- InterPro:   IPR010240
- InterPro:   IPR017772
- InterPro:   IPR016454
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF00266 Aminotran_5 [H]

EC number: =2.8.1.7 [H]

Molecular weight: Translated: 42613; Mature: 42613

Theoretical pI: Translated: 6.08; Mature: 6.08

Prosite motif: PS00595 AA_TRANSFER_CLASS_5

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEAYLDNSATTKCAAEVVETAVKVMSEDYGNPSSKHMKGVDAEKYIREAKEVIAKTLKCQ
CCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCHHHHCCCCHHHHHHHHHHHHHHHHCCC
DKEILFTSGGTESNNMAIIGTAMANKRKGMHCIVSSVEHSSVKEPFNFLEKEGFRITYLP
CCEEEEECCCCCCCCEEEEEECHHCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEE
VDKDGIVDIEALKDALDDETILVSVMYVNNEIGAVEPIEEIGHIIKDYNPDIVYHVDAIQ
CCCCCCEEHHHHHHHCCCCEEEEEEEEECCCCCCCCCHHHHHHHHHCCCCCEEEEEHHHH
AYGKYKIYPKKLGIDLLSVSGHKIHGPKGSGFLFINDKTRIKPIIYGGGQQKGMRSGTEN
HCCCEEEEHHHHCEEEEECCCCEEECCCCCEEEEECCCCEEEEEEECCCCCCCCCCCCCC
VPAIAGLATAVKLIYNNDFESKIAHLYELKDYFIDELEKLPDVQVNSKKGTDSAPQIVSA
CCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCEECCCCCCCCCCHHHHH
SFKGVRAEVLLHSLEDKGIYVSSGSACSSNKPGLSNTLVAIGLDKELLDSTLRFSFCYET
HHCCHHHHHHHHHHCCCCEEEECCCCCCCCCCCCCCCEEEEECCHHHHHHHHHHHHHHHH
TKEELEYTIETLKQLLPMLRKYTRR
HHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MEAYLDNSATTKCAAEVVETAVKVMSEDYGNPSSKHMKGVDAEKYIREAKEVIAKTLKCQ
CCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCHHHHCCCCHHHHHHHHHHHHHHHHCCC
DKEILFTSGGTESNNMAIIGTAMANKRKGMHCIVSSVEHSSVKEPFNFLEKEGFRITYLP
CCEEEEECCCCCCCCEEEEEECHHCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEE
VDKDGIVDIEALKDALDDETILVSVMYVNNEIGAVEPIEEIGHIIKDYNPDIVYHVDAIQ
CCCCCCEEHHHHHHHCCCCEEEEEEEEECCCCCCCCCHHHHHHHHHCCCCCEEEEEHHHH
AYGKYKIYPKKLGIDLLSVSGHKIHGPKGSGFLFINDKTRIKPIIYGGGQQKGMRSGTEN
HCCCEEEEHHHHCEEEEECCCCEEECCCCCEEEEECCCCEEEEEEECCCCCCCCCCCCCC
VPAIAGLATAVKLIYNNDFESKIAHLYELKDYFIDELEKLPDVQVNSKKGTDSAPQIVSA
CCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCEECCCCCCCCCCHHHHH
SFKGVRAEVLLHSLEDKGIYVSSGSACSSNKPGLSNTLVAIGLDKELLDSTLRFSFCYET
HHCCHHHHHHHHHHCCCCEEEECCCCCCCCCCCCCCCEEEEECCHHHHHHHHHHHHHHHH
TKEELEYTIETLKQLLPMLRKYTRR
HHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA