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The map label for this gene is thiI

Identifier: 238917090

GI number: 238917090

Start: 1171866

End: 1173047

Strand: Reverse

Name: thiI

Synonym: EUBELI_01160

Alternate gene names: 238917090

Gene position: 1173047-1171866 (Counterclockwise)

Preceding gene: 238917091

Following gene: 238917088

Centisome position: 54.71

GC content: 37.9

Gene sequence:

>1182_bases
ATGTATAAAGCATTTTTGATAAAGTATGGAGAAATAGGCGTTAAAGGAAAGAACAGATTCATCTTTGAGGACGCCCTTGT
AAGACAGATTAAGTTCTCACTTAAAGATGTTGAGGGAGAATTTGATGTAAGAAGAGCAGATGGAAGAATCTATGTTAATG
CACTTGCGGACTATGATTATGATGAGGTAATTGAGAGCCTTACAAGAGTATTTGGTATAGTTGGAATCTGTCCTGTAGTT
CAGATTGAAGATAACGGTTTTGATGACCTTGCTAATCAGGTTATCAATTATCTTGATAAGGCATATAAGAATAAGAATCT
TACATTCAAGGTTAATGCAAGAAGAACAAGAAAGAATTATCCAATGAACTCAATGGAGATTAATATGGAACTTGGTGGAA
GAATTCTTGATGCATTCCCAGAGATGAAGGTTGACGTGCATAAGCCTGAGGTTCTTTTACAGGTAGAAATCAGAGGAGAT
GTAATCAATATTTACTCTATCGAGGTTCCGGGTCCTGGCGGAATGCCAATCGGAACAGCAGGAAAGGCTATGCTTTTATT
ATCAGGTGGTATTGACAGCCCTGTTGCAGGATATATGGTTGCCAAAAGAGGTGTACAGATTGAAGCAACATACTTCCATG
CTCCTCCATATACAAGTGAAAGAGCAAAGCAGAAGGTAATTGACCTTGCAAAGATTGTATCTAAGTATTCAGGCCCTATT
ACACTTAATGTTGTTAATTTCACAGATATCCAGATGGCTATATATGAGAAATGTCCACATGATGAGCTTACAATCATTAT
GAGAAGATATATGATGAAGATTGCTGAAGATTTAGGAAAATCAAGCGGATGTCAGGGACTTGTTACAGGAGAGAGTATCG
GACAGGTTGCAAGCCAGACAATGGCAAGTCTTTACTGTACTAACGAAGTGTGTACAATGCCTGTATTCAGACCGGTTATC
GGTTTTGACAAGCAGGAGATTATTGATATATCAGAAAAGATTGGCTCATATGAGACATCAATACAGCCATTTGAGGACTG
CTGTACAATATTCGTTGCAAAGCACCCTGTAACCAAGCCTAATCTTAATATTATCAAGCAGCATGAGACTAACCTTGACG
GAGTTATTGAGGAGCTTTACAAGACAGCAATTGAGACAACAGAAAAAATCGTAATTGAATAA

Upstream 100 bases:

>100_bases
TTAGGAAGTACACAAGACGATAATTGTGTGAAAATAGATTTTCACACACAAGATTTGTGCTTTGCACAAATCTTGATATG
CAAAGGAAAGGCGGAAAACG

Downstream 100 bases:

>100_bases
AATAAAACCGCTTTATCTGTAACAGATAAAGCGGTTTATATGTGCTTACACATCATTACTCAACGATGTATGGCTTTAAT
ACGGCAAGGATTGTATCAAT

Product: thiamine biosynthesis protein ThiI

Products: NA

Alternate protein names: Sulfur carrier protein ThiS sulfurtransferase; Thiamine biosynthesis protein thiI; tRNA 4-thiouridine synthase

Number of amino acids: Translated: 393; Mature: 393

Protein sequence:

>393_residues
MYKAFLIKYGEIGVKGKNRFIFEDALVRQIKFSLKDVEGEFDVRRADGRIYVNALADYDYDEVIESLTRVFGIVGICPVV
QIEDNGFDDLANQVINYLDKAYKNKNLTFKVNARRTRKNYPMNSMEINMELGGRILDAFPEMKVDVHKPEVLLQVEIRGD
VINIYSIEVPGPGGMPIGTAGKAMLLLSGGIDSPVAGYMVAKRGVQIEATYFHAPPYTSERAKQKVIDLAKIVSKYSGPI
TLNVVNFTDIQMAIYEKCPHDELTIIMRRYMMKIAEDLGKSSGCQGLVTGESIGQVASQTMASLYCTNEVCTMPVFRPVI
GFDKQEIIDISEKIGSYETSIQPFEDCCTIFVAKHPVTKPNLNIIKQHETNLDGVIEELYKTAIETTEKIVIE

Sequences:

>Translated_393_residues
MYKAFLIKYGEIGVKGKNRFIFEDALVRQIKFSLKDVEGEFDVRRADGRIYVNALADYDYDEVIESLTRVFGIVGICPVV
QIEDNGFDDLANQVINYLDKAYKNKNLTFKVNARRTRKNYPMNSMEINMELGGRILDAFPEMKVDVHKPEVLLQVEIRGD
VINIYSIEVPGPGGMPIGTAGKAMLLLSGGIDSPVAGYMVAKRGVQIEATYFHAPPYTSERAKQKVIDLAKIVSKYSGPI
TLNVVNFTDIQMAIYEKCPHDELTIIMRRYMMKIAEDLGKSSGCQGLVTGESIGQVASQTMASLYCTNEVCTMPVFRPVI
GFDKQEIIDISEKIGSYETSIQPFEDCCTIFVAKHPVTKPNLNIIKQHETNLDGVIEELYKTAIETTEKIVIE
>Mature_393_residues
MYKAFLIKYGEIGVKGKNRFIFEDALVRQIKFSLKDVEGEFDVRRADGRIYVNALADYDYDEVIESLTRVFGIVGICPVV
QIEDNGFDDLANQVINYLDKAYKNKNLTFKVNARRTRKNYPMNSMEINMELGGRILDAFPEMKVDVHKPEVLLQVEIRGD
VINIYSIEVPGPGGMPIGTAGKAMLLLSGGIDSPVAGYMVAKRGVQIEATYFHAPPYTSERAKQKVIDLAKIVSKYSGPI
TLNVVNFTDIQMAIYEKCPHDELTIIMRRYMMKIAEDLGKSSGCQGLVTGESIGQVASQTMASLYCTNEVCTMPVFRPVI
GFDKQEIIDISEKIGSYETSIQPFEDCCTIFVAKHPVTKPNLNIIKQHETNLDGVIEELYKTAIETTEKIVIE

Specific function: Catalyzes the ATP-dependent transfer of a sulfur to tRNA to produce 4-thiouridine in position 8 of tRNAs, which functions as a near-UV photosensor. Also catalyzes the transfer of sulfur to the sulfur carrier protein ThiS, forming ThiS-thiocarboxylate. Thi

COG id: COG0301

COG function: function code H; Thiamine biosynthesis ATP pyrophosphatase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 THUMP domain

Homologues:

Organism=Escherichia coli, GI1786625, Length=385, Percent_Identity=28.5714285714286, Blast_Score=143, Evalue=1e-35,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): THII_EUBE2 (C4Z0P7)

Other databases:

- EMBL:   CP001104
- RefSeq:   YP_002930607.1
- GeneID:   7957704
- GenomeReviews:   CP001104_GR
- KEGG:   eel:EUBELI_01160
- OMA:   ESIGQVA
- ProtClustDB:   CLSK2506189
- GO:   GO:0005737
- HAMAP:   MF_00021
- InterPro:   IPR014729
- InterPro:   IPR003720
- InterPro:   IPR020536
- InterPro:   IPR004114
- Gene3D:   G3DSA:3.40.50.620
- SMART:   SM00981
- TIGRFAMs:   TIGR00342

Pfam domain/function: PF02568 ThiI; PF02926 THUMP

EC number: =2.8.1.4

Molecular weight: Translated: 44033; Mature: 44033

Theoretical pI: Translated: 5.21; Mature: 5.21

Prosite motif: PS51165 THUMP

Important sites: BINDING 268-268 BINDING 290-290 BINDING 299-299

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
5.3 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
5.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MYKAFLIKYGEIGVKGKNRFIFEDALVRQIKFSLKDVEGEFDVRRADGRIYVNALADYDY
CCEEEEEEECCEEECCCCEEEHHHHHHHHHHHHHHHCCCCEEEEECCCEEEEEEECCCCH
DEVIESLTRVFGIVGICPVVQIEDNGFDDLANQVINYLDKAYKNKNLTFKVNARRTRKNY
HHHHHHHHHHHHHHHCCEEEEECCCCHHHHHHHHHHHHHHHHCCCCEEEEEECCHHCCCC
PMNSMEINMELGGRILDAFPEMKVDVHKPEVLLQVEIRGDVINIYSIEVPGPGGMPIGTA
CCCCEEEEHHHCCHHHHCCCCCEEEECCCEEEEEEEECCCEEEEEEEEECCCCCCCCCCC
GKAMLLLSGGIDSPVAGYMVAKRGVQIEATYFHAPPYTSERAKQKVIDLAKIVSKYSGPI
CCEEEEEECCCCCCHHHHHHHHCCEEEEEEEEECCCCCHHHHHHHHHHHHHHHHHCCCCE
TLNVVNFTDIQMAIYEKCPHDELTIIMRRYMMKIAEDLGKSSGCQGLVTGESIGQVASQT
EEEEEEECHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCCCCCEEEECHHHHHHHHHH
MASLYCTNEVCTMPVFRPVIGFDKQEIIDISEKIGSYETSIQPFEDCCTIFVAKHPVTKP
HHHHHCCCCHHCCHHHHHHHCCCHHHHHHHHHHHCCCCCCCCCHHHHHEEEEECCCCCCC
NLNIIKQHETNLDGVIEELYKTAIETTEKIVIE
CCCEEEECCCCHHHHHHHHHHHHHHHHHHEECC
>Mature Secondary Structure
MYKAFLIKYGEIGVKGKNRFIFEDALVRQIKFSLKDVEGEFDVRRADGRIYVNALADYDY
CCEEEEEEECCEEECCCCEEEHHHHHHHHHHHHHHHCCCCEEEEECCCEEEEEEECCCCH
DEVIESLTRVFGIVGICPVVQIEDNGFDDLANQVINYLDKAYKNKNLTFKVNARRTRKNY
HHHHHHHHHHHHHHHCCEEEEECCCCHHHHHHHHHHHHHHHHCCCCEEEEEECCHHCCCC
PMNSMEINMELGGRILDAFPEMKVDVHKPEVLLQVEIRGDVINIYSIEVPGPGGMPIGTA
CCCCEEEEHHHCCHHHHCCCCCEEEECCCEEEEEEEECCCEEEEEEEEECCCCCCCCCCC
GKAMLLLSGGIDSPVAGYMVAKRGVQIEATYFHAPPYTSERAKQKVIDLAKIVSKYSGPI
CCEEEEEECCCCCCHHHHHHHHCCEEEEEEEEECCCCCHHHHHHHHHHHHHHHHHCCCCE
TLNVVNFTDIQMAIYEKCPHDELTIIMRRYMMKIAEDLGKSSGCQGLVTGESIGQVASQT
EEEEEEECHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCCCCCEEEECHHHHHHHHHH
MASLYCTNEVCTMPVFRPVIGFDKQEIIDISEKIGSYETSIQPFEDCCTIFVAKHPVTKP
HHHHHCCCCHHCCHHHHHHHCCCHHHHHHHHHHHCCCCCCCCCHHHHHEEEEECCCCCCC
NLNIIKQHETNLDGVIEELYKTAIETTEKIVIE
CCCEEEECCCCHHHHHHHHHHHHHHHHHHEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA