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The map label for this gene is polA [H]

Identifier: 238917003

GI number: 238917003

Start: 1083887

End: 1086571

Strand: Reverse

Name: polA [H]

Synonym: EUBELI_01072

Alternate gene names: 238917003

Gene position: 1086571-1083887 (Counterclockwise)

Preceding gene: 238917004

Following gene: 238917002

Centisome position: 50.68

GC content: 36.16

Gene sequence:

>2685_bases
ATGAGTGAAAAACTTATATTAATAGATGGACACAGCATATTAAATAGGGCATTTTACGGAGTTCCTGACCTTACTAATGC
AGAAGGCATACATACCAATGCAATGTATGGCTTTCTTAATATTTTATTTAAATTTCTTGATGAAGAAAAGCCTGACTATA
TTACGGTTGCATTTGATTTATCTGCACCGACATTCAGACATAAGGTGTTTGATGGATATAAAGGAACAAGAAAGCCTATG
CAGCCAGAACTAAAGCAGCAGGTTCCTCTTATGAAGGAGCTGCTTAAAGCAATGAATATTACAGTTGTCGAGCAGGAGGG
ATACGAAGCTGATGATATTCTAGGTACAATTGCGAAGGAAAGTGCTGCGAAAGGAATAGATGTATCAATTGTTTCAGGTG
ACAGAGATTTACTTCAGCTTGCAGATGAGCATATTAAGATAAGAATACCGAAGACAAAGAAAGGCGTAACAGAGGTTGAA
GATTATTATCCATCAGATGTATTTAATCTGTATGGAGTTACTCCTCATGAATTTATAGATGTTAAAGCACTTATGGGCGA
TACATCTGATAATATACCGGGAGCACCGGGAGTAGGACCTAAGACAGCGTCGGCTATTATTCAGAAATATCATAGTCTTG
ATAATGTTTTTGAACATCTTTGCGAGTTGAAGCCGCCTAAAGCAAAAATATCAATAACTGAGAATGTCGAGCAGATTAAG
ATGAGCAGATTCTTGTCAGAGATTAAAATCGATGTTCCGATTGATTATAAAGTTGAAGATTCTAAAGCAGGTGATTTCTT
TAATGAGAATTCATATGAACTTTTTAAAAAGTATAATTTTAAAAATATGCTAAAGAAGTTTGAGAATACAGATATAATTG
CTAAAGACCCTGAATGTTATGAATATTTCAAGAAGATATCTGATTTTGCTGATGTTGAAAATGTATTTAACAAAGCGGTG
GATATAGCAGGTGGGATTGAAAAAATCGGACTTTCTATTGTAAGAGAAAATGAACTGATTGCAGTTGGAATAACACTTTC
AGATACAGAAATATATTATATCCCGGTATCGGGTTTTGTTACAGAGAGTTATCTTGCAGACCGTCTGTCAGATGTTGTAA
GCGTGGCTTCTAACAGAAATATAGCATCTGCCAATATTAAAGATTATCTTGATATATTTGAAAAAGACAAGATAAATGGC
TATCCGCTTGTTTCAGAAAAAGCATTTATTGATACTGCAATTGCGGCATATCTGCTTCATCCAAGTAACGAAAGTTATGA
TTATGAAAGTCTTGGAAGAGAGTTTTTATCATTAACATATCCTTCAAAAACAGAACTTCTGGGTAAATTGTCAATTAATA
AGGCAGTAAATGAAGCGGAAGACAATCTTATTAAGTATGCATGTCTTTCATCATATGCTTATTACAAATGTGCTGATAAA
ATTACAGAACAGTTAAAGAGTGAGAATATGTATGAGCTGTTTGAAACTATAGAAATGCCTCTTATATTTGTTCTTTTTGA
AATGCAGCAACAGGGCATAAGTGTTGATAAACAGGCACTTGTAGATTATTCAAAGGTACTTGGAACTAAGATTTCAGTCC
TTGAGAAAGAGATATATGAAGCAGCAGGCGAAGAGTTCAATATCAATTCACCAAAGCAGCTTGGTGTTATACTTTTTGAA
AGGCTTGGAATGCCTAACGGAAAGAAGACAAAGAGTGGTTATTCAACAGCTGCAGACGTACTTGAAAAGCTTGCACCTGA
TTATCCGGTTGTAAGTAAGATTCTTGAATACAGACAATTAGCAAAGCTTAAATCAACTTATGCTGACGGACTTACACAGT
ATATATCAGAAGATGGAAGGATTCATGGTACATTTAACCAGACAATAACTGCAACTGGAAGAATAAGCAGTACAGAGCCT
AATCTTCAGAATATTCCTATAAGAATGGAGATGGGTAAGGCTATAAGAAAGGTATTTGTTCCAAAGGATGGCTTTGTATT
TTTGGATGCCGATTACTCTCAGATAGAGTTAAGAATACTTGCTCATATGTCAGGGGATGAGAAGCTCATTGAGGCGTATA
ATTCATCAGCAGATATCCACAGAGCAACGGCGGCACAAGTCTTTGGCGTACCGCTCGAAGAGGTTACTGATGAACAGAGA
AGAAATGCCAAGGCAGTTAATTTCGGCATTATCTATGGAATGAGTTCGTTTGGATTAAGCCAGGATCTTAGTATTTCAAG
GAAAGAAGCTAAAGAATATATTGAAAGATATTTTGCAAGCTATCCGACTATCAAGACATTTATAGACGGACTCGTAAGTG
ATGCCAAAGAAAAAGGATATTCACTTACAATGTATAACAGAAGAAGAGAAATTCCTGAGATTAAATCTTCTAATTTCATG
CAGCGTTCTTTTGGAGAGAGAGTTGCCATGAATGCACCAATTCAGGGAACTGCAGCCGATATTATCAAGCTGGCAATGAT
TAATGTATATAATGCACTTAAGGAGCATAATCTCAGGTCAAGACTCATATTGCAGGTTCATGATGAGCTTCTTGTTGAGA
CGGCAGAGGATGAGGTTGATACTGTAAAGCAGATTATGCTTGATGGAATGAAGAATGCAGTAAGCCTTAAGGTACCTCTT
GAGGTAGATTTAAAGGAAGGAAAAGACTGGCTGGAGGCACACTGA

Upstream 100 bases:

>100_bases
TCATTTTACCAGAGTAAGATATATTTTTGTTTCTACAGTTATGATTCTTACAGCCTTGCTTCTTATCATTATTAAATTCT
TTTAAAGATGGGAGATTGTT

Downstream 100 bases:

>100_bases
TGAAAGTTATTGGAATCACAGGTGGTGTAGGAGCAGGAAAATCTTCTGTTCTTGCACTCCTTAAAGAAATGTGTTCCTGC
GACATTATACAGGCAGACCT

Product: DNA polymerase I

Products: NA

Alternate protein names: POL I [H]

Number of amino acids: Translated: 894; Mature: 893

Protein sequence:

>894_residues
MSEKLILIDGHSILNRAFYGVPDLTNAEGIHTNAMYGFLNILFKFLDEEKPDYITVAFDLSAPTFRHKVFDGYKGTRKPM
QPELKQQVPLMKELLKAMNITVVEQEGYEADDILGTIAKESAAKGIDVSIVSGDRDLLQLADEHIKIRIPKTKKGVTEVE
DYYPSDVFNLYGVTPHEFIDVKALMGDTSDNIPGAPGVGPKTASAIIQKYHSLDNVFEHLCELKPPKAKISITENVEQIK
MSRFLSEIKIDVPIDYKVEDSKAGDFFNENSYELFKKYNFKNMLKKFENTDIIAKDPECYEYFKKISDFADVENVFNKAV
DIAGGIEKIGLSIVRENELIAVGITLSDTEIYYIPVSGFVTESYLADRLSDVVSVASNRNIASANIKDYLDIFEKDKING
YPLVSEKAFIDTAIAAYLLHPSNESYDYESLGREFLSLTYPSKTELLGKLSINKAVNEAEDNLIKYACLSSYAYYKCADK
ITEQLKSENMYELFETIEMPLIFVLFEMQQQGISVDKQALVDYSKVLGTKISVLEKEIYEAAGEEFNINSPKQLGVILFE
RLGMPNGKKTKSGYSTAADVLEKLAPDYPVVSKILEYRQLAKLKSTYADGLTQYISEDGRIHGTFNQTITATGRISSTEP
NLQNIPIRMEMGKAIRKVFVPKDGFVFLDADYSQIELRILAHMSGDEKLIEAYNSSADIHRATAAQVFGVPLEEVTDEQR
RNAKAVNFGIIYGMSSFGLSQDLSISRKEAKEYIERYFASYPTIKTFIDGLVSDAKEKGYSLTMYNRRREIPEIKSSNFM
QRSFGERVAMNAPIQGTAADIIKLAMINVYNALKEHNLRSRLILQVHDELLVETAEDEVDTVKQIMLDGMKNAVSLKVPL
EVDLKEGKDWLEAH

Sequences:

>Translated_894_residues
MSEKLILIDGHSILNRAFYGVPDLTNAEGIHTNAMYGFLNILFKFLDEEKPDYITVAFDLSAPTFRHKVFDGYKGTRKPM
QPELKQQVPLMKELLKAMNITVVEQEGYEADDILGTIAKESAAKGIDVSIVSGDRDLLQLADEHIKIRIPKTKKGVTEVE
DYYPSDVFNLYGVTPHEFIDVKALMGDTSDNIPGAPGVGPKTASAIIQKYHSLDNVFEHLCELKPPKAKISITENVEQIK
MSRFLSEIKIDVPIDYKVEDSKAGDFFNENSYELFKKYNFKNMLKKFENTDIIAKDPECYEYFKKISDFADVENVFNKAV
DIAGGIEKIGLSIVRENELIAVGITLSDTEIYYIPVSGFVTESYLADRLSDVVSVASNRNIASANIKDYLDIFEKDKING
YPLVSEKAFIDTAIAAYLLHPSNESYDYESLGREFLSLTYPSKTELLGKLSINKAVNEAEDNLIKYACLSSYAYYKCADK
ITEQLKSENMYELFETIEMPLIFVLFEMQQQGISVDKQALVDYSKVLGTKISVLEKEIYEAAGEEFNINSPKQLGVILFE
RLGMPNGKKTKSGYSTAADVLEKLAPDYPVVSKILEYRQLAKLKSTYADGLTQYISEDGRIHGTFNQTITATGRISSTEP
NLQNIPIRMEMGKAIRKVFVPKDGFVFLDADYSQIELRILAHMSGDEKLIEAYNSSADIHRATAAQVFGVPLEEVTDEQR
RNAKAVNFGIIYGMSSFGLSQDLSISRKEAKEYIERYFASYPTIKTFIDGLVSDAKEKGYSLTMYNRRREIPEIKSSNFM
QRSFGERVAMNAPIQGTAADIIKLAMINVYNALKEHNLRSRLILQVHDELLVETAEDEVDTVKQIMLDGMKNAVSLKVPL
EVDLKEGKDWLEAH
>Mature_893_residues
SEKLILIDGHSILNRAFYGVPDLTNAEGIHTNAMYGFLNILFKFLDEEKPDYITVAFDLSAPTFRHKVFDGYKGTRKPMQ
PELKQQVPLMKELLKAMNITVVEQEGYEADDILGTIAKESAAKGIDVSIVSGDRDLLQLADEHIKIRIPKTKKGVTEVED
YYPSDVFNLYGVTPHEFIDVKALMGDTSDNIPGAPGVGPKTASAIIQKYHSLDNVFEHLCELKPPKAKISITENVEQIKM
SRFLSEIKIDVPIDYKVEDSKAGDFFNENSYELFKKYNFKNMLKKFENTDIIAKDPECYEYFKKISDFADVENVFNKAVD
IAGGIEKIGLSIVRENELIAVGITLSDTEIYYIPVSGFVTESYLADRLSDVVSVASNRNIASANIKDYLDIFEKDKINGY
PLVSEKAFIDTAIAAYLLHPSNESYDYESLGREFLSLTYPSKTELLGKLSINKAVNEAEDNLIKYACLSSYAYYKCADKI
TEQLKSENMYELFETIEMPLIFVLFEMQQQGISVDKQALVDYSKVLGTKISVLEKEIYEAAGEEFNINSPKQLGVILFER
LGMPNGKKTKSGYSTAADVLEKLAPDYPVVSKILEYRQLAKLKSTYADGLTQYISEDGRIHGTFNQTITATGRISSTEPN
LQNIPIRMEMGKAIRKVFVPKDGFVFLDADYSQIELRILAHMSGDEKLIEAYNSSADIHRATAAQVFGVPLEEVTDEQRR
NAKAVNFGIIYGMSSFGLSQDLSISRKEAKEYIERYFASYPTIKTFIDGLVSDAKEKGYSLTMYNRRREIPEIKSSNFMQ
RSFGERVAMNAPIQGTAADIIKLAMINVYNALKEHNLRSRLILQVHDELLVETAEDEVDTVKQIMLDGMKNAVSLKVPLE
VDLKEGKDWLEAH

Specific function: In addition to polymerase activity, this DNA polymerase exhibits 3' to 5' and 5' to 3' exonuclease activity [H]

COG id: COG0749

COG function: function code L; DNA polymerase I - 3'-5' exonuclease and polymerase domains

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 5'-3' exonuclease domain [H]

Homologues:

Organism=Homo sapiens, GI112420958, Length=544, Percent_Identity=32.9044117647059, Blast_Score=275, Evalue=1e-73,
Organism=Homo sapiens, GI139394648, Length=608, Percent_Identity=29.4407894736842, Blast_Score=243, Evalue=9e-64,
Organism=Escherichia coli, GI1790294, Length=940, Percent_Identity=39.1489361702128, Blast_Score=576, Evalue=1e-165,
Organism=Escherichia coli, GI226510970, Length=265, Percent_Identity=23.7735849056604, Blast_Score=90, Evalue=7e-19,
Organism=Caenorhabditis elegans, GI86563272, Length=406, Percent_Identity=32.512315270936, Blast_Score=196, Evalue=4e-50,
Organism=Drosophila melanogaster, GI17933644, Length=419, Percent_Identity=33.890214797136, Blast_Score=218, Evalue=2e-56,

Paralogues:

None

Copy number: 400 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002562
- InterPro:   IPR020046
- InterPro:   IPR020045
- InterPro:   IPR002421
- InterPro:   IPR020047
- InterPro:   IPR019760
- InterPro:   IPR001098
- InterPro:   IPR018320
- InterPro:   IPR002298
- InterPro:   IPR008918
- InterPro:   IPR003583
- InterPro:   IPR012337 [H]

Pfam domain/function: PF01367 5_3_exonuc; PF02739 5_3_exonuc_N; PF00476 DNA_pol_A [H]

EC number: =2.7.7.7 [H]

Molecular weight: Translated: 100681; Mature: 100550

Theoretical pI: Translated: 4.86; Mature: 4.86

Prosite motif: PS00447 DNA_POLYMERASE_A

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSEKLILIDGHSILNRAFYGVPDLTNAEGIHTNAMYGFLNILFKFLDEEKPDYITVAFDL
CCCCEEEEECHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCEEEEEEEC
SAPTFRHKVFDGYKGTRKPMQPELKQQVPLMKELLKAMNITVVEQEGYEADDILGTIAKE
CCCHHHHHHHCCCCCCCCCCCCHHHHHCHHHHHHHHHCCEEEEECCCCCHHHHHHHHHHH
SAAKGIDVSIVSGDRDLLQLADEHIKIRIPKTKKGVTEVEDYYPSDVFNLYGVTPHEFID
HHCCCCEEEEECCCHHHHHHCCCCEEEECCCCCCCCHHHHHCCCCCHHHHCCCCHHHHHH
VKALMGDTSDNIPGAPGVGPKTASAIIQKYHSLDNVFEHLCELKPPKAKISITENVEQIK
HHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEHHHHHHHH
MSRFLSEIKIDVPIDYKVEDSKAGDFFNENSYELFKKYNFKNMLKKFENTDIIAKDPECY
HHHHHHHCEECCCCCEEECCCCCCCCCCCCHHHHHHHCCHHHHHHHHCCCCCEECCCHHH
EYFKKISDFADVENVFNKAVDIAGGIEKIGLSIVRENELIAVGITLSDTEIYYIPVSGFV
HHHHHHHHHHHHHHHHHHHHHHCCCHHHHCHHEEECCCEEEEEEEEECCEEEEEECCCHH
TESYLADRLSDVVSVASNRNIASANIKDYLDIFEKDKINGYPLVSEKAFIDTAIAAYLLH
HHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHCC
PSNESYDYESLGREFLSLTYPSKTELLGKLSINKAVNEAEDNLIKYACLSSYAYYKCADK
CCCCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ITEQLKSENMYELFETIEMPLIFVLFEMQQQGISVDKQALVDYSKVLGTKISVLEKEIYE
HHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
AAGEEFNINSPKQLGVILFERLGMPNGKKTKSGYSTAADVLEKLAPDYPVVSKILEYRQL
HCCCCCCCCCCHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHH
AKLKSTYADGLTQYISEDGRIHGTFNQTITATGRISSTEPNLQNIPIRMEMGKAIRKVFV
HHHHHHHHHHHHHHHCCCCCEEEECCCEEEECCCCCCCCCCCCCCCEEEHHHHHHHHHCC
PKDGFVFLDADYSQIELRILAHMSGDEKLIEAYNSSADIHRATAAQVFGVPLEEVTDEQR
CCCCEEEEECCCCCEEEEEEEECCCCHHHHHHHCCCCHHHHHHHHHHHCCCHHHHHHHHH
RNAKAVNFGIIYGMSSFGLSQDLSISRKEAKEYIERYFASYPTIKTFIDGLVSDAKEKGY
CCCCEEEEEEEECCHHCCCCCCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCC
SLTMYNRRREIPEIKSSNFMQRSFGERVAMNAPIQGTAADIIKLAMINVYNALKEHNLRS
EEEEEHHHCCCCCCCCCCHHHHHHCCCEEECCCCCCHHHHHHHHHHHHHHHHHHHCCHHH
RLILQVHDELLVETAEDEVDTVKQIMLDGMKNAVSLKVPLEVDLKEGKDWLEAH
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECEEEEHHCCCHHHCCC
>Mature Secondary Structure 
SEKLILIDGHSILNRAFYGVPDLTNAEGIHTNAMYGFLNILFKFLDEEKPDYITVAFDL
CCCEEEEECHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCEEEEEEEC
SAPTFRHKVFDGYKGTRKPMQPELKQQVPLMKELLKAMNITVVEQEGYEADDILGTIAKE
CCCHHHHHHHCCCCCCCCCCCCHHHHHCHHHHHHHHHCCEEEEECCCCCHHHHHHHHHHH
SAAKGIDVSIVSGDRDLLQLADEHIKIRIPKTKKGVTEVEDYYPSDVFNLYGVTPHEFID
HHCCCCEEEEECCCHHHHHHCCCCEEEECCCCCCCCHHHHHCCCCCHHHHCCCCHHHHHH
VKALMGDTSDNIPGAPGVGPKTASAIIQKYHSLDNVFEHLCELKPPKAKISITENVEQIK
HHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEHHHHHHHH
MSRFLSEIKIDVPIDYKVEDSKAGDFFNENSYELFKKYNFKNMLKKFENTDIIAKDPECY
HHHHHHHCEECCCCCEEECCCCCCCCCCCCHHHHHHHCCHHHHHHHHCCCCCEECCCHHH
EYFKKISDFADVENVFNKAVDIAGGIEKIGLSIVRENELIAVGITLSDTEIYYIPVSGFV
HHHHHHHHHHHHHHHHHHHHHHCCCHHHHCHHEEECCCEEEEEEEEECCEEEEEECCCHH
TESYLADRLSDVVSVASNRNIASANIKDYLDIFEKDKINGYPLVSEKAFIDTAIAAYLLH
HHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHCC
PSNESYDYESLGREFLSLTYPSKTELLGKLSINKAVNEAEDNLIKYACLSSYAYYKCADK
CCCCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ITEQLKSENMYELFETIEMPLIFVLFEMQQQGISVDKQALVDYSKVLGTKISVLEKEIYE
HHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
AAGEEFNINSPKQLGVILFERLGMPNGKKTKSGYSTAADVLEKLAPDYPVVSKILEYRQL
HCCCCCCCCCCHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHH
AKLKSTYADGLTQYISEDGRIHGTFNQTITATGRISSTEPNLQNIPIRMEMGKAIRKVFV
HHHHHHHHHHHHHHHCCCCCEEEECCCEEEECCCCCCCCCCCCCCCEEEHHHHHHHHHCC
PKDGFVFLDADYSQIELRILAHMSGDEKLIEAYNSSADIHRATAAQVFGVPLEEVTDEQR
CCCCEEEEECCCCCEEEEEEEECCCCHHHHHHHCCCCHHHHHHHHHHHCCCHHHHHHHHH
RNAKAVNFGIIYGMSSFGLSQDLSISRKEAKEYIERYFASYPTIKTFIDGLVSDAKEKGY
CCCCEEEEEEEECCHHCCCCCCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCC
SLTMYNRRREIPEIKSSNFMQRSFGERVAMNAPIQGTAADIIKLAMINVYNALKEHNLRS
EEEEEHHHCCCCCCCCCCHHHHHHCCCEEECCCCCCHHHHHHHHHHHHHHHHHHHCCHHH
RLILQVHDELLVETAEDEVDTVKQIMLDGMKNAVSLKVPLEVDLKEGKDWLEAH
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECEEEEHHCCCHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9387221; 9384377 [H]