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The map label for this gene is rsmF [H]

Identifier: 238916962

GI number: 238916962

Start: 1044074

End: 1045402

Strand: Reverse

Name: rsmF [H]

Synonym: EUBELI_01031

Alternate gene names: 238916962

Gene position: 1045402-1044074 (Counterclockwise)

Preceding gene: 238916963

Following gene: 238916961

Centisome position: 48.76

GC content: 38.53

Gene sequence:

>1329_bases
ATGAAAATGATGTTAGGTGATGAATATGACAGCTATATAGAAAGCTTTAATGATAAGAGATTATATGGACTGCGTGTCAA
TAACCTTAAAATTTCAACAGAGGATTTCCTTAAGATATGTCCGTTTAAGTTAGAACCTGTTCCTTGGATTGAGAACGGAT
TCTATTATTCAGAAGATGACAAGCCTGCTAAACACCCATATTATTTTGCAGGCTTATATTATATTCAGGAGCCAAGTGCC
ATGACACCGGCAAATGTTCTTCCTATTGATGAGGGAGATGTTGTGTTCGATATGTGTGCAGCTCCCGGAGGCAAGTCAAC
AGAGCTTGCAGCCAAATTAAACAAAACAGGACTACTTATAACCAATGATATAAGCAATTCGCGTGCCAAAGCACTTCTTA
AGAATGTTGAAGTATTCGGTGTTCCTAATCTGTGCGTCCTTAATGAAGACCCTGTGGGTATAGCTTCAAGATTCTCAGGA
TTCTTTGACAAAGTACTTATTGATGCTCCATGCTCAGGTGAAGGAATGTTCAGAAAAGACAATAAGCTTATCAAAGCATG
GGAAAAGAATGGTCCTGAGTTCTATTCGCAGATTCAGAGGAATATCATTCTTGCCGGTGCTGACATGTTAAAACCGGGCG
GTAAATTATTATATTCTACATGCACATTTTCAAAGCTGGAGGATGAAGATTCAGTAATACATCTTCTTACCAACAGACCT
GATATGCACCTTATAGATATCAAGCCATATGAAGGCTTCTCACACGGCTTTGATACTGATGAAGGATATCATCTTGAGAA
GGCTGTCAGAATATTTCCTCATAAGATGCCTGGAGAAGGACATTTTGTGGCATTATTTGAGAAAGACGGCGAAGATTATA
CATCTTCTAAAAGACCTGTATCAGGAAAGACAAAGCTTCCTGATGAATTAAAGGAGTTCATGGACAGCACAACATTTGAA
TATGACCCTGCTTATATCAATATAAGAGATACAAGGGTCTTTCTTACATCTCCTTATATGGCAGAGGAACGCGGTTTAAG
AATTATAAGAAATGGTCTGCTGCTAGGTGAACTTAAGAAGAACCGTTTTGAACCGAGTCAGGCATTTGCAATGGCACTTA
CTAATGATCAGTTTAATAACTGTCTTGACCTTTCTGTTTCAGATGACAGAGTAATCAGATATCTTAAAGGAGAGACTATC
GATATTGATGATTTTAATGTCAAATCTGGCTGGACACTTGTATGTGTTGATGGCTATCCACTTGGATGGGGCAAGAACGC
TAACGGACAGTTAAAAAATAAATATCTTGCAGGCTGGAGATGGATGTAA

Upstream 100 bases:

>100_bases
GCTGAGGATTACCGGGCACAGTCAGTGCAGTAACTCATTACAGAAACTATACAGAATTGAGGTATACAAAGTGAATTTAC
CAGAGAAATATACAGAATCA

Downstream 100 bases:

>100_bases
ATGCTTATAAGACTTGATAAATATCTTGCAGACATGCAGGTTGGTACACGCAGTGAAGTTAAAGAACTGATAAGAAAAAA
GAGAGTCACTGTTAATGACA

Product: hypothetical protein

Products: NA

Alternate protein names: 16S rRNA m5C1407 methyltransferase; rRNA (cytosine-C(5)-)-methyltransferase rsmF [H]

Number of amino acids: Translated: 442; Mature: 442

Protein sequence:

>442_residues
MKMMLGDEYDSYIESFNDKRLYGLRVNNLKISTEDFLKICPFKLEPVPWIENGFYYSEDDKPAKHPYYFAGLYYIQEPSA
MTPANVLPIDEGDVVFDMCAAPGGKSTELAAKLNKTGLLITNDISNSRAKALLKNVEVFGVPNLCVLNEDPVGIASRFSG
FFDKVLIDAPCSGEGMFRKDNKLIKAWEKNGPEFYSQIQRNIILAGADMLKPGGKLLYSTCTFSKLEDEDSVIHLLTNRP
DMHLIDIKPYEGFSHGFDTDEGYHLEKAVRIFPHKMPGEGHFVALFEKDGEDYTSSKRPVSGKTKLPDELKEFMDSTTFE
YDPAYINIRDTRVFLTSPYMAEERGLRIIRNGLLLGELKKNRFEPSQAFAMALTNDQFNNCLDLSVSDDRVIRYLKGETI
DIDDFNVKSGWTLVCVDGYPLGWGKNANGQLKNKYLAGWRWM

Sequences:

>Translated_442_residues
MKMMLGDEYDSYIESFNDKRLYGLRVNNLKISTEDFLKICPFKLEPVPWIENGFYYSEDDKPAKHPYYFAGLYYIQEPSA
MTPANVLPIDEGDVVFDMCAAPGGKSTELAAKLNKTGLLITNDISNSRAKALLKNVEVFGVPNLCVLNEDPVGIASRFSG
FFDKVLIDAPCSGEGMFRKDNKLIKAWEKNGPEFYSQIQRNIILAGADMLKPGGKLLYSTCTFSKLEDEDSVIHLLTNRP
DMHLIDIKPYEGFSHGFDTDEGYHLEKAVRIFPHKMPGEGHFVALFEKDGEDYTSSKRPVSGKTKLPDELKEFMDSTTFE
YDPAYINIRDTRVFLTSPYMAEERGLRIIRNGLLLGELKKNRFEPSQAFAMALTNDQFNNCLDLSVSDDRVIRYLKGETI
DIDDFNVKSGWTLVCVDGYPLGWGKNANGQLKNKYLAGWRWM
>Mature_442_residues
MKMMLGDEYDSYIESFNDKRLYGLRVNNLKISTEDFLKICPFKLEPVPWIENGFYYSEDDKPAKHPYYFAGLYYIQEPSA
MTPANVLPIDEGDVVFDMCAAPGGKSTELAAKLNKTGLLITNDISNSRAKALLKNVEVFGVPNLCVLNEDPVGIASRFSG
FFDKVLIDAPCSGEGMFRKDNKLIKAWEKNGPEFYSQIQRNIILAGADMLKPGGKLLYSTCTFSKLEDEDSVIHLLTNRP
DMHLIDIKPYEGFSHGFDTDEGYHLEKAVRIFPHKMPGEGHFVALFEKDGEDYTSSKRPVSGKTKLPDELKEFMDSTTFE
YDPAYINIRDTRVFLTSPYMAEERGLRIIRNGLLLGELKKNRFEPSQAFAMALTNDQFNNCLDLSVSDDRVIRYLKGETI
DIDDFNVKSGWTLVCVDGYPLGWGKNANGQLKNKYLAGWRWM

Specific function: Specifically methylates the cytosine at position 1407 (m5C1407) of 16S rRNA [H]

COG id: COG0144

COG function: function code J; tRNA and rRNA cytosine-C5-methylases

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. RsmB/NOP family [H]

Homologues:

Organism=Homo sapiens, GI76150625, Length=293, Percent_Identity=29.6928327645051, Blast_Score=122, Evalue=7e-28,
Organism=Homo sapiens, GI76150623, Length=293, Percent_Identity=29.6928327645051, Blast_Score=122, Evalue=7e-28,
Organism=Homo sapiens, GI39995082, Length=306, Percent_Identity=25.8169934640523, Blast_Score=105, Evalue=1e-22,
Organism=Homo sapiens, GI301336155, Length=306, Percent_Identity=25.8169934640523, Blast_Score=104, Evalue=1e-22,
Organism=Homo sapiens, GI32698918, Length=193, Percent_Identity=31.6062176165803, Blast_Score=93, Evalue=6e-19,
Organism=Homo sapiens, GI11545785, Length=186, Percent_Identity=33.3333333333333, Blast_Score=88, Evalue=2e-17,
Organism=Homo sapiens, GI40316918, Length=283, Percent_Identity=27.208480565371, Blast_Score=84, Evalue=2e-16,
Organism=Homo sapiens, GI8922322, Length=234, Percent_Identity=26.9230769230769, Blast_Score=74, Evalue=3e-13,
Organism=Homo sapiens, GI23199998, Length=234, Percent_Identity=26.9230769230769, Blast_Score=74, Evalue=3e-13,
Organism=Homo sapiens, GI270288818, Length=234, Percent_Identity=26.9230769230769, Blast_Score=74, Evalue=3e-13,
Organism=Homo sapiens, GI270288816, Length=234, Percent_Identity=26.9230769230769, Blast_Score=74, Evalue=4e-13,
Organism=Escherichia coli, GI87081985, Length=449, Percent_Identity=28.5077951002227, Blast_Score=173, Evalue=2e-44,
Organism=Escherichia coli, GI2367212, Length=247, Percent_Identity=25.5060728744939, Blast_Score=77, Evalue=3e-15,
Organism=Caenorhabditis elegans, GI17536757, Length=299, Percent_Identity=30.7692307692308, Blast_Score=133, Evalue=1e-31,
Organism=Caenorhabditis elegans, GI25143471, Length=290, Percent_Identity=28.9655172413793, Blast_Score=95, Evalue=8e-20,
Organism=Saccharomyces cerevisiae, GI6324268, Length=296, Percent_Identity=31.7567567567568, Blast_Score=139, Evalue=1e-33,
Organism=Saccharomyces cerevisiae, GI6319447, Length=277, Percent_Identity=27.7978339350181, Blast_Score=108, Evalue=2e-24,
Organism=Drosophila melanogaster, GI22024126, Length=304, Percent_Identity=29.9342105263158, Blast_Score=115, Evalue=5e-26,
Organism=Drosophila melanogaster, GI21355201, Length=315, Percent_Identity=28.8888888888889, Blast_Score=111, Evalue=1e-24,
Organism=Drosophila melanogaster, GI24668781, Length=233, Percent_Identity=28.755364806867, Blast_Score=82, Evalue=1e-15,
Organism=Drosophila melanogaster, GI21356513, Length=236, Percent_Identity=28.3898305084746, Blast_Score=81, Evalue=2e-15,
Organism=Drosophila melanogaster, GI19920494, Length=172, Percent_Identity=27.3255813953488, Blast_Score=73, Evalue=4e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001678
- InterPro:   IPR011023 [H]

Pfam domain/function: PF01189 Nol1_Nop2_Fmu [H]

EC number: =2.1.1.178 [H]

Molecular weight: Translated: 50150; Mature: 50150

Theoretical pI: Translated: 5.06; Mature: 5.06

Prosite motif: PS01153 NOL1_NOP2_SUN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKMMLGDEYDSYIESFNDKRLYGLRVNNLKISTEDFLKICPFKLEPVPWIENGFYYSEDD
CCCCCCCCHHHHHHCCCCCEEEEEEECCEEECHHHHHHHCCCCCCCCCEECCCEEECCCC
KPAKHPYYFAGLYYIQEPSAMTPANVLPIDEGDVVFDMCAAPGGKSTELAAKLNKTGLLI
CCCCCCEEEEEEEEEECCCCCCCCCEEECCCCCEEEEEECCCCCCCCHHHEECCCCEEEE
TNDISNSRAKALLKNVEVFGVPNLCVLNEDPVGIASRFSGFFDKVLIDAPCSGEGMFRKD
EECCCCHHHHHHHHCCEEECCCCEEEECCCCCCHHHHHHHHHHHEEEECCCCCCCCEECC
NKLIKAWEKNGPEFYSQIQRNIILAGADMLKPGGKLLYSTCTFSKLEDEDSVIHLLTNRP
CHHHHHHHCCCHHHHHHHHHCEEEECHHHCCCCCCEEEEEECCCCCCCCCCEEEEEECCC
DMHLIDIKPYEGFSHGFDTDEGYHLEKAVRIFPHKMPGEGHFVALFEKDGEDYTSSKRPV
CEEEEEECCCCCCCCCCCCCCCEEHHHHHHHCCCCCCCCCCEEEEEECCCCCCCCCCCCC
SGKTKLPDELKEFMDSTTFEYDPAYINIRDTRVFLTSPYMAEERGLRIIRNGLLLGELKK
CCCCCCHHHHHHHHHCCCEEECCCEEEEEEEEEEEECCCCCHHCCHHEECCCEEEEEHHH
NRFEPSQAFAMALTNDQFNNCLDLSVSDDRVIRYLKGETIDIDDFNVKSGWTLVCVDGYP
CCCCCCCEEEEEECCCCCCCEEEECCCHHHHHHHHCCCEEECCCCCCCCCCEEEEECCEE
LGWGKNANGQLKNKYLAGWRWM
CCCCCCCCCCCCCEEECCEECC
>Mature Secondary Structure
MKMMLGDEYDSYIESFNDKRLYGLRVNNLKISTEDFLKICPFKLEPVPWIENGFYYSEDD
CCCCCCCCHHHHHHCCCCCEEEEEEECCEEECHHHHHHHCCCCCCCCCEECCCEEECCCC
KPAKHPYYFAGLYYIQEPSAMTPANVLPIDEGDVVFDMCAAPGGKSTELAAKLNKTGLLI
CCCCCCEEEEEEEEEECCCCCCCCCEEECCCCCEEEEEECCCCCCCCHHHEECCCCEEEE
TNDISNSRAKALLKNVEVFGVPNLCVLNEDPVGIASRFSGFFDKVLIDAPCSGEGMFRKD
EECCCCHHHHHHHHCCEEECCCCEEEECCCCCCHHHHHHHHHHHEEEECCCCCCCCEECC
NKLIKAWEKNGPEFYSQIQRNIILAGADMLKPGGKLLYSTCTFSKLEDEDSVIHLLTNRP
CHHHHHHHCCCHHHHHHHHHCEEEECHHHCCCCCCEEEEEECCCCCCCCCCEEEEEECCC
DMHLIDIKPYEGFSHGFDTDEGYHLEKAVRIFPHKMPGEGHFVALFEKDGEDYTSSKRPV
CEEEEEECCCCCCCCCCCCCCCEEHHHHHHHCCCCCCCCCCEEEEEECCCCCCCCCCCCC
SGKTKLPDELKEFMDSTTFEYDPAYINIRDTRVFLTSPYMAEERGLRIIRNGLLLGELKK
CCCCCCHHHHHHHHHCCCEEECCCEEEEEEEEEEEECCCCCHHCCHHEECCCEEEEEHHH
NRFEPSQAFAMALTNDQFNNCLDLSVSDDRVIRYLKGETIDIDDFNVKSGWTLVCVDGYP
CCCCCCCEEEEEECCCCCCCEEEECCCHHHHHHHHCCCEEECCCCCCCCCCEEEEECCEE
LGWGKNANGQLKNKYLAGWRWM
CCCCCCCCCCCCCEEECCEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA