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The map label for this gene is ytzG [H]

Identifier: 238916961

GI number: 238916961

Start: 1043339

End: 1044073

Strand: Reverse

Name: ytzG [H]

Synonym: EUBELI_01030

Alternate gene names: 238916961

Gene position: 1044073-1043339 (Counterclockwise)

Preceding gene: 238916962

Following gene: 238916960

Centisome position: 48.69

GC content: 35.37

Gene sequence:

>735_bases
ATGCTTATAAGACTTGATAAATATCTTGCAGACATGCAGGTTGGTACACGCAGTGAAGTTAAAGAACTGATAAGAAAAAA
GAGAGTCACTGTTAATGACAATATTGTGACAAAGCCTGAATCAAAGATTAATACAGATGGTGATTCTGTAAATGTAGATG
GTAATGCTGTGGGCTATCATGAATATGAGTATTTTATGCTTAACAAGCCTGCCGGTGTTGTTTCAGCAACTGATGACAAT
ACATGTACAACAGTGATTGACCTTATTAAAAATAAGAACAGAAAAGACCTTTTTCCTGTAGGAAGACTTGATAAGGACAC
CGAAGGGCTTCTTATAATTACGAATGATGGTGCTATGGCACATGATCTGCTATCACCTAAAAAGCATGTTGATAAGACTT
ATTATGCCAAAGTTAAAGGTAAGATAAGTGATATGGAAGTTAAGCAGTTTGCTGATGGACTATTCGTTGATGAAGAGCTG
ACAGCACTTCCTGCCATATTAAAGGTATTAAGCTATAATTCTGATAAGGATTATTCTGAGATTCATGTAACAATACATGA
GGGTAAGTTTCATCAGGTAAAGAGAATGTTTACTGCCATAGGTTCAGAGGTTCTGTTCCTTAAGCGTATAACATTTGGTG
CATTACCACTTGATGAAAGCCTAAAAACAGGTGAATACAGAGCACTTACCAAAGAAGAGATATCAATATTACAACGAAAC
CAAATTAAAAGATAA

Upstream 100 bases:

>100_bases
CTGGACACTTGTATGTGTTGATGGCTATCCACTTGGATGGGGCAAGAACGCTAACGGACAGTTAAAAAATAAATATCTTG
CAGGCTGGAGATGGATGTAA

Downstream 100 bases:

>100_bases
GGAGAATTCCATGGTTAAGGATTTTCTTCCAATTAGCAGAGAAGATATGAAAAAAAGGGGCTGGCAGCAATGCGACTTCG
TGTATATATGCGGAGATGCC

Product: ribosomal small subunit pseudouridine synthase A

Products: pseudouridine 5'-phosphate; H2O [C]

Alternate protein names: RNA pseudouridylate synthase; RNA-uridine isomerase [H]

Number of amino acids: Translated: 244; Mature: 244

Protein sequence:

>244_residues
MLIRLDKYLADMQVGTRSEVKELIRKKRVTVNDNIVTKPESKINTDGDSVNVDGNAVGYHEYEYFMLNKPAGVVSATDDN
TCTTVIDLIKNKNRKDLFPVGRLDKDTEGLLIITNDGAMAHDLLSPKKHVDKTYYAKVKGKISDMEVKQFADGLFVDEEL
TALPAILKVLSYNSDKDYSEIHVTIHEGKFHQVKRMFTAIGSEVLFLKRITFGALPLDESLKTGEYRALTKEEISILQRN
QIKR

Sequences:

>Translated_244_residues
MLIRLDKYLADMQVGTRSEVKELIRKKRVTVNDNIVTKPESKINTDGDSVNVDGNAVGYHEYEYFMLNKPAGVVSATDDN
TCTTVIDLIKNKNRKDLFPVGRLDKDTEGLLIITNDGAMAHDLLSPKKHVDKTYYAKVKGKISDMEVKQFADGLFVDEEL
TALPAILKVLSYNSDKDYSEIHVTIHEGKFHQVKRMFTAIGSEVLFLKRITFGALPLDESLKTGEYRALTKEEISILQRN
QIKR
>Mature_244_residues
MLIRLDKYLADMQVGTRSEVKELIRKKRVTVNDNIVTKPESKINTDGDSVNVDGNAVGYHEYEYFMLNKPAGVVSATDDN
TCTTVIDLIKNKNRKDLFPVGRLDKDTEGLLIITNDGAMAHDLLSPKKHVDKTYYAKVKGKISDMEVKQFADGLFVDEEL
TALPAILKVLSYNSDKDYSEIHVTIHEGKFHQVKRMFTAIGSEVLFLKRITFGALPLDESLKTGEYRALTKEEISILQRN
QIKR

Specific function: Responsible For Synthesis Of Pseudouridine From Uracil-516 In 16s Ribosomal RNA. [C]

COG id: COG1187

COG function: function code J; 16S rRNA uridine-516 pseudouridylate synthase and related pseudouridylate synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S4 RNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1788510, Length=236, Percent_Identity=40.2542372881356, Blast_Score=162, Evalue=3e-41,
Organism=Escherichia coli, GI1787524, Length=236, Percent_Identity=28.3898305084746, Blast_Score=105, Evalue=4e-24,
Organism=Escherichia coli, GI1790453, Length=247, Percent_Identity=28.3400809716599, Blast_Score=92, Evalue=3e-20,
Organism=Escherichia coli, GI87081838, Length=178, Percent_Identity=30.8988764044944, Blast_Score=80, Evalue=1e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020103
- InterPro:   IPR006145
- InterPro:   IPR000748
- InterPro:   IPR018496
- InterPro:   IPR002942 [H]

Pfam domain/function: PF00849 PseudoU_synth_2; PF01479 S4 [H]

EC number: 4.2.1.70 [C]

Molecular weight: Translated: 27612; Mature: 27612

Theoretical pI: Translated: 7.75; Mature: 7.75

Prosite motif: PS50889 S4 ; PS01149 PSI_RSU

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLIRLDKYLADMQVGTRSEVKELIRKKRVTVNDNIVTKPESKINTDGDSVNVDGNAVGYH
CEEEHHHHHHHHCCCCHHHHHHHHHHCCCEECCCEEECCCCCCCCCCCEEECCCCEEEEE
EYEYFMLNKPAGVVSATDDNTCTTVIDLIKNKNRKDLFPVGRLDKDTEGLLIITNDGAMA
EEEEEEEECCCCEEEECCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCCEEEEECCCCHH
HDLLSPKKHVDKTYYAKVKGKISDMEVKQFADGLFVDEELTALPAILKVLSYNSDKDYSE
HHHCCCHHHHCHHEEEEECCCCCHHHHHHHHCCCEECHHHHHHHHHHHHHHCCCCCCCEE
IHVTIHEGKFHQVKRMFTAIGSEVLFLKRITFGALPLDESLKTGEYRALTKEEISILQRN
EEEEEECCCHHHHHHHHHHHCCHHEEHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHH
QIKR
HCCC
>Mature Secondary Structure
MLIRLDKYLADMQVGTRSEVKELIRKKRVTVNDNIVTKPESKINTDGDSVNVDGNAVGYH
CEEEHHHHHHHHCCCCHHHHHHHHHHCCCEECCCEEECCCCCCCCCCCEEECCCCEEEEE
EYEYFMLNKPAGVVSATDDNTCTTVIDLIKNKNRKDLFPVGRLDKDTEGLLIITNDGAMA
EEEEEEEECCCCEEEECCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCCEEEEECCCCHH
HDLLSPKKHVDKTYYAKVKGKISDMEVKQFADGLFVDEELTALPAILKVLSYNSDKDYSE
HHHCCCHHHHCHHEEEEECCCCCHHHHHHHHCCCEECHHHHHHHHHHHHHHCCCCCCCEE
IHVTIHEGKFHQVKRMFTAIGSEVLFLKRITFGALPLDESLKTGEYRALTKEEISILQRN
EEEEEECCCHHHHHHHHHHHCCHHEEHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHH
QIKR
HCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: uracil; D-ribose phosphate [C]

Specific reaction: uracil + D-ribose phosphate = pseudouridine 5'-phosphate + H2O [C]

General reaction: addition of H2O; elimination of H2O; C-O bond cleavage [C]

Inhibitor: 1-(Tetrahydro-2-furanyl)-5-fluorouracil; 5-fluorouracil [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]