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The map label for this gene is hisZ [H]

Identifier: 238916955

GI number: 238916955

Start: 1037091

End: 1038371

Strand: Reverse

Name: hisZ [H]

Synonym: EUBELI_01024

Alternate gene names: 238916955

Gene position: 1038371-1037091 (Counterclockwise)

Preceding gene: 238916956

Following gene: 238916954

Centisome position: 48.43

GC content: 36.22

Gene sequence:

>1281_bases
ATGAAGAAAGATTTACTACATACACCAGAGGGTGTACGCGATATATACAGTACAGAATGTGCAGAAAAGAAGGTCATTGA
GAATCGTCTTCATGATGTCTGTGCATCATATGGCTATAACGATATACAGACACCTTCTATTGAATTCTTTGATGTATTCA
ATAAAGAACGTGGAAGTGTACCGTCTAATGAAATGTTCAAGCTTTTTGACAGATACGGAAACACTTTAGTATTAAGACCG
GATATGACACCTTCCATTGCAAGAGCAACAGCAAAATATTTTGAAGACAGAGTTCTTCCTGTCAAGCTCTGTTATCTTGG
CAATACATTTGTCAATGTAAGCAAGTATTATCAGGGAAGACTTAAAGAAAATACGATGCTTGGTGCAGAGCTTATCAATG
ATAACTCTCTGGCAGCGGATTATGAGATTATCTCTATGGTTATTGACTGCATGTTATCAGCAGGTCTTACTGAATTTCAG
GTTGAGCTTGGTAACGTTGCTTTCTTTAATGGACTTTTACATAAAGCTGGCATTACAGGAGATTCTGCAGAAGAATTATT
AAGACTTATCAAGGATAAGAATTACTTCGGTGTTGAAGAACTCTTAGATAGTCTTAATATTGATGATGCAGTTGCCAAAG
CATTGCTTGAACTTCCTCAGTTATTTGGTGGTACAGAGGTGCTTGAAAAGGCTAAATCTTTAACAGATGAAGAAGAGTGC
ATACAGGCTGTTAGCAGACTTGAGGAGTTGTATGAGCTTCTTAAGAATAACAATTACGATAAATATATCTCATTTGATTT
AGGAGATTTATCTGAACATGCATACTATACAGGTATTATTTTCCATGCATATACATTTGGAACTGGTGAGCCCGTTGTAA
ATGGCGGACGTTATGACAAGCTGCTTGGACAGTTTGGCTGTGACAAGGCATCTATCGGTTTCTCTATAACAATCGACAGA
CTTATAGCTGCACTTAACAGACAGAATATCCATATTCCACATGATGCCAACGGAACACTTCTTGTATACAATGCTGACAG
GCTTAGTGATGCTATAAATGTATCTAAGAAATTAAGAAGCACTGGCGTTAATGTCTGCATGATTGAATATAAAGACAGCA
AGTCTGATTCTCAATATATTGAATATGCTAAGGAATCATCATTGGTAAATGTAGCATTTATTGATGATGAATACACTTCT
GATTCAACAATAAGAGTTATTGATGAATCAGGAAAAAATGAAAGAGCAGCTGTAAAAAGCCTTTTAGGAGGTACATTTTA
A

Upstream 100 bases:

>100_bases
ATAACTACATATTTATGTATAATTCATACAAATTTAGTGTGTTAGCATAGTATCACTTCAACGCATTAAAGCATTCAATA
TAACGAAAGGATATACATTT

Downstream 100 bases:

>100_bases
TGAGATATATAACTATTGCATTAGCAAAAGGCCGTCTTGCAAAGAAGGCAATGGAGATATTTGAACAGATTGGTATTCCT
TGCGAAGAGATGAAGGATGA

Product: histidyl-tRNA synthetase

Products: AMP; diphosphate +L-histidyl-tRNAHis [C]

Alternate protein names: NA

Number of amino acids: Translated: 426; Mature: 426

Protein sequence:

>426_residues
MKKDLLHTPEGVRDIYSTECAEKKVIENRLHDVCASYGYNDIQTPSIEFFDVFNKERGSVPSNEMFKLFDRYGNTLVLRP
DMTPSIARATAKYFEDRVLPVKLCYLGNTFVNVSKYYQGRLKENTMLGAELINDNSLAADYEIISMVIDCMLSAGLTEFQ
VELGNVAFFNGLLHKAGITGDSAEELLRLIKDKNYFGVEELLDSLNIDDAVAKALLELPQLFGGTEVLEKAKSLTDEEEC
IQAVSRLEELYELLKNNNYDKYISFDLGDLSEHAYYTGIIFHAYTFGTGEPVVNGGRYDKLLGQFGCDKASIGFSITIDR
LIAALNRQNIHIPHDANGTLLVYNADRLSDAINVSKKLRSTGVNVCMIEYKDSKSDSQYIEYAKESSLVNVAFIDDEYTS
DSTIRVIDESGKNERAAVKSLLGGTF

Sequences:

>Translated_426_residues
MKKDLLHTPEGVRDIYSTECAEKKVIENRLHDVCASYGYNDIQTPSIEFFDVFNKERGSVPSNEMFKLFDRYGNTLVLRP
DMTPSIARATAKYFEDRVLPVKLCYLGNTFVNVSKYYQGRLKENTMLGAELINDNSLAADYEIISMVIDCMLSAGLTEFQ
VELGNVAFFNGLLHKAGITGDSAEELLRLIKDKNYFGVEELLDSLNIDDAVAKALLELPQLFGGTEVLEKAKSLTDEEEC
IQAVSRLEELYELLKNNNYDKYISFDLGDLSEHAYYTGIIFHAYTFGTGEPVVNGGRYDKLLGQFGCDKASIGFSITIDR
LIAALNRQNIHIPHDANGTLLVYNADRLSDAINVSKKLRSTGVNVCMIEYKDSKSDSQYIEYAKESSLVNVAFIDDEYTS
DSTIRVIDESGKNERAAVKSLLGGTF
>Mature_426_residues
MKKDLLHTPEGVRDIYSTECAEKKVIENRLHDVCASYGYNDIQTPSIEFFDVFNKERGSVPSNEMFKLFDRYGNTLVLRP
DMTPSIARATAKYFEDRVLPVKLCYLGNTFVNVSKYYQGRLKENTMLGAELINDNSLAADYEIISMVIDCMLSAGLTEFQ
VELGNVAFFNGLLHKAGITGDSAEELLRLIKDKNYFGVEELLDSLNIDDAVAKALLELPQLFGGTEVLEKAKSLTDEEEC
IQAVSRLEELYELLKNNNYDKYISFDLGDLSEHAYYTGIIFHAYTFGTGEPVVNGGRYDKLLGQFGCDKASIGFSITIDR
LIAALNRQNIHIPHDANGTLLVYNADRLSDAINVSKKLRSTGVNVCMIEYKDSKSDSQYIEYAKESSLVNVAFIDDEYTS
DSTIRVIDESGKNERAAVKSLLGGTF

Specific function: Required for the first step of histidine biosynthesis. May allow the feedback regulation of ATP phosphoribosyltransferase activity by histidine [H]

COG id: COG3705

COG function: function code E; ATP phosphoribosyltransferase involved in histidine biosynthesis

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family. HisZ subfamily [H]

Homologues:

Organism=Homo sapiens, GI6996014, Length=452, Percent_Identity=22.1238938053097, Blast_Score=88, Evalue=2e-17,
Organism=Homo sapiens, GI15029520, Length=439, Percent_Identity=21.1845102505695, Blast_Score=80, Evalue=3e-15,
Organism=Saccharomyces cerevisiae, GI6325290, Length=444, Percent_Identity=20.7207207207207, Blast_Score=75, Evalue=1e-14,
Organism=Drosophila melanogaster, GI24643061, Length=359, Percent_Identity=24.2339832869081, Blast_Score=89, Evalue=4e-18,
Organism=Drosophila melanogaster, GI24643059, Length=377, Percent_Identity=23.0769230769231, Blast_Score=79, Evalue=5e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002314
- InterPro:   IPR006195
- InterPro:   IPR004154
- InterPro:   IPR004517
- InterPro:   IPR004516 [H]

Pfam domain/function: PF00587 tRNA-synt_2b [H]

EC number: 6.1.1.21 [C]

Molecular weight: Translated: 47674; Mature: 47674

Theoretical pI: Translated: 4.54; Mature: 4.54

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKDLLHTPEGVRDIYSTECAEKKVIENRLHDVCASYGYNDIQTPSIEFFDVFNKERGSV
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHCCCCCCC
PSNEMFKLFDRYGNTLVLRPDMTPSIARATAKYFEDRVLPVKLCYLGNTFVNVSKYYQGR
CHHHHHHHHHHHCCEEEECCCCCHHHHHHHHHHHHCCCCEEEEEECCCHHHHHHHHHHCC
LKENTMLGAELINDNSLAADYEIISMVIDCMLSAGLTEFQVELGNVAFFNGLLHKAGITG
CCCCHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCCEEHHHHCCCHHHHHHHHHCCCCC
DSAEELLRLIKDKNYFGVEELLDSLNIDDAVAKALLELPQLFGGTEVLEKAKSLTDEEEC
CCHHHHHHHHHCCCCCCHHHHHHHCCCCHHHHHHHHHHHHHCCCHHHHHHHHCCCCHHHH
IQAVSRLEELYELLKNNNYDKYISFDLGDLSEHAYYTGIIFHAYTFGTGEPVVNGGRYDK
HHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCHHHHEEEEEEEEECCCCCCCCCCCHHHH
LLGQFGCDKASIGFSITIDRLIAALNRQNIHIPHDANGTLLVYNADRLSDAINVSKKLRS
HHHHHCCCCCCCCCEEEHHHHHHHHCCCCEECCCCCCCEEEEEEHHHHHHHHHHHHHHHH
TGVNVCMIEYKDSKSDSQYIEYAKESSLVNVAFIDDEYTSDSTIRVIDESGKNERAAVKS
CCCCEEEEEECCCCCHHHHHHHHHHCCEEEEEEECCCCCCCCEEEEEECCCCCHHHHHHH
LLGGTF
HHCCCC
>Mature Secondary Structure
MKKDLLHTPEGVRDIYSTECAEKKVIENRLHDVCASYGYNDIQTPSIEFFDVFNKERGSV
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHCCCCCCC
PSNEMFKLFDRYGNTLVLRPDMTPSIARATAKYFEDRVLPVKLCYLGNTFVNVSKYYQGR
CHHHHHHHHHHHCCEEEECCCCCHHHHHHHHHHHHCCCCEEEEEECCCHHHHHHHHHHCC
LKENTMLGAELINDNSLAADYEIISMVIDCMLSAGLTEFQVELGNVAFFNGLLHKAGITG
CCCCHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCCEEHHHHCCCHHHHHHHHHCCCCC
DSAEELLRLIKDKNYFGVEELLDSLNIDDAVAKALLELPQLFGGTEVLEKAKSLTDEEEC
CCHHHHHHHHHCCCCCCHHHHHHHCCCCHHHHHHHHHHHHHCCCHHHHHHHHCCCCHHHH
IQAVSRLEELYELLKNNNYDKYISFDLGDLSEHAYYTGIIFHAYTFGTGEPVVNGGRYDK
HHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCHHHHEEEEEEEEECCCCCCCCCCCHHHH
LLGQFGCDKASIGFSITIDRLIAALNRQNIHIPHDANGTLLVYNADRLSDAINVSKKLRS
HHHHHCCCCCCCCCEEEHHHHHHHHCCCCEECCCCCCCEEEEEEHHHHHHHHHHHHHHHH
TGVNVCMIEYKDSKSDSQYIEYAKESSLVNVAFIDDEYTSDSTIRVIDESGKNERAAVKS
CCCCEEEEEECCCCCHHHHHHHHHHCCEEEEEEECCCCCCCCEEEEEECCCCCHHHHHHH
LLGGTF
HHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: Mg2+ [C]

Kcat value (1/min): 8520 [C]

Specific activity: 121.8

Km value (mM): 0.06 {His}} 0.046 {His}} 0.03 {His}} 0.02 {His}} 0.017 {His}} 0.011 {His}} 0.008 {His}} 0.0014 {tRNAHis}} 0.00056 {tRNAHis}} 0.0016 {U73tRNAHisGUG}} 0.00031 {U73tRNAHisGUG}} 0.89 {ATP}} 0.74 {ATP}} 0.655 {ATP}} 0.5

Substrates: ATP; @HIST01.txt*L-histidine!; tRNAHis [C]

Specific reaction: ATP + @HIST01.txt*L-histidine! + tRNAHis = AMP + diphosphate +L-histidyl-tRNAHis [C]

General reaction: Aminoacylation; Esterification [C]

Inhibitor: 1, 2, 4-Triazole-3-alanine; D-Histidine; Diphosphate; L-Histidinol; Mg2+; N-Acetyl histidine; NEM; p-Chloromercuribenzoate [C]

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA