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The map label for this gene is miaB [H]

Identifier: 238916920

GI number: 238916920

Start: 1005019

End: 1006440

Strand: Direct

Name: miaB [H]

Synonym: EUBELI_00989

Alternate gene names: 238916920

Gene position: 1005019-1006440 (Clockwise)

Preceding gene: 238916919

Following gene: 238916921

Centisome position: 46.87

GC content: 37.2

Gene sequence:

>1422_bases
ATGCAGGAAGATAATAACACCAGTGCACTTGAAGAACAGCTTTATTATATTGGAGAATGTAAGAAGTTCCTGGAAAAGAA
AACAAAAGAAATAGGCAGACCGTTATATGCGTGTGTACACACATTTGGCTGCCAGATGAATGCCAGAGATTCTGAGAAGC
TTCTCGGGGTACTTAAAGAGATTGGATATCTTGAGACAGAGGATGAGGATAAGTCAGATTTTGTTATATATAATACATGT
ACTGTAAGAGAGAATGCTAATCTTAAGGTTTATGGACGTCTTGGACATCTTAAGAATGTCAAAAGAAAGAATCCGCATAT
GCTTATTGCAATGTGTGGCTGTATGATGCAGGAGCCTGATGTGGTCGAGAAGATAAGAGATTCTTATAAGTTTGTTGATA
TTGTTTTTGGTACATTTAATATATATGCAATGGCAAAGCTTATATATAACAGAATTACATCAGGAAGCCAGGTTATTGAT
ATCTGGGAAAAGACAAAGGATATTGTTGAGGAGCTTCCTACAGAAAGAAAGTTCCCGTTCAAATCAGGTGTTAATATTAT
GTACGGCTGTAATAATTTCTGTACTTACTGTATCGTGCCTTATGTACGAGGAAGAGAGATAAGCCGTGAGCCTAAGGATA
TCATTATGGAGATTGAAAGGCTTGTAAAGGATGGCGTCAAGGAAGTTATGCTTCTTGGACAAAATGTTGATTCTTATGGA
AAGACACTTGACAATCCTGTATCATTTGCGCAGCTTCTAAGAGAGATTGATAAGATAGAGGGACTTGAAAGAATCAGATT
CATGACACCTCATCCTAAGGATATTGAGGAAGAGACTCTTGAAGTTATAAGAGATTCCAAGAAAATATGTAATCATATAC
ATTTTCCACTTCAGTCAGGAAGCTCAAGGCTTCTTAAGCTGATGAACAGAAAGTATACTAAAGAGCATTACTTAGAGCAG
GTTGAGATGATAAGAAGAATACTGCCCGATGTTTCTATTACAACTGATATTATTGTTGGGTTTCCAGGAGAGACAGAGGA
AGATTTCGAGGATACTATTGATGTTGTAAAGAAAGCAAAGTTTGATAGTGCATATACATTTATATATTCAAAAAGAACAG
GTACGCCTGCTGCCAGAATGGAGAATCAGGTGCCTGAGGATGTTGTTAAGGACAGATTTAACAGACTGCTTGCAACTGTC
AATGAAGTGTCACATGAGCATATCAGAAGATATGAGGGCATGGATATGAAAGTCTTGGTTGAGGGTAAAGATGACCATGA
AGAAGGTTTTGTTACAGGCAGAATGACTAATAATATTCTTGTACATTTCGCAGGGGATGAATCACTTATCGGACAGATTG
TTACAGTACATCTTGACGAATGTAAAGGATTTTACTATATGGGCAGACTTATCGAGGATTAA

Upstream 100 bases:

>100_bases
GGGATACTATTATGGTAAACCGGTATGCTTACAAGATTTTGAAGAACAGCACTTGAAAAGATTTGTATTAGGATAAGTAT
AGAATGGAGAATTGTAGATA

Downstream 100 bases:

>100_bases
TCAGGGAGTAATATAATGGCAGAGTATTCACCAATGATGCAGCATTATCTTGCTACTAAAGCAGAGTACAAGGACTGCAT
ACTTTTTTACAGACTTGGAG

Product: 2-alkenal reductase

Products: NA

Alternate protein names: tRNA-i(6)A37 methylthiotransferase [H]

Number of amino acids: Translated: 473; Mature: 473

Protein sequence:

>473_residues
MQEDNNTSALEEQLYYIGECKKFLEKKTKEIGRPLYACVHTFGCQMNARDSEKLLGVLKEIGYLETEDEDKSDFVIYNTC
TVRENANLKVYGRLGHLKNVKRKNPHMLIAMCGCMMQEPDVVEKIRDSYKFVDIVFGTFNIYAMAKLIYNRITSGSQVID
IWEKTKDIVEELPTERKFPFKSGVNIMYGCNNFCTYCIVPYVRGREISREPKDIIMEIERLVKDGVKEVMLLGQNVDSYG
KTLDNPVSFAQLLREIDKIEGLERIRFMTPHPKDIEEETLEVIRDSKKICNHIHFPLQSGSSRLLKLMNRKYTKEHYLEQ
VEMIRRILPDVSITTDIIVGFPGETEEDFEDTIDVVKKAKFDSAYTFIYSKRTGTPAARMENQVPEDVVKDRFNRLLATV
NEVSHEHIRRYEGMDMKVLVEGKDDHEEGFVTGRMTNNILVHFAGDESLIGQIVTVHLDECKGFYYMGRLIED

Sequences:

>Translated_473_residues
MQEDNNTSALEEQLYYIGECKKFLEKKTKEIGRPLYACVHTFGCQMNARDSEKLLGVLKEIGYLETEDEDKSDFVIYNTC
TVRENANLKVYGRLGHLKNVKRKNPHMLIAMCGCMMQEPDVVEKIRDSYKFVDIVFGTFNIYAMAKLIYNRITSGSQVID
IWEKTKDIVEELPTERKFPFKSGVNIMYGCNNFCTYCIVPYVRGREISREPKDIIMEIERLVKDGVKEVMLLGQNVDSYG
KTLDNPVSFAQLLREIDKIEGLERIRFMTPHPKDIEEETLEVIRDSKKICNHIHFPLQSGSSRLLKLMNRKYTKEHYLEQ
VEMIRRILPDVSITTDIIVGFPGETEEDFEDTIDVVKKAKFDSAYTFIYSKRTGTPAARMENQVPEDVVKDRFNRLLATV
NEVSHEHIRRYEGMDMKVLVEGKDDHEEGFVTGRMTNNILVHFAGDESLIGQIVTVHLDECKGFYYMGRLIED
>Mature_473_residues
MQEDNNTSALEEQLYYIGECKKFLEKKTKEIGRPLYACVHTFGCQMNARDSEKLLGVLKEIGYLETEDEDKSDFVIYNTC
TVRENANLKVYGRLGHLKNVKRKNPHMLIAMCGCMMQEPDVVEKIRDSYKFVDIVFGTFNIYAMAKLIYNRITSGSQVID
IWEKTKDIVEELPTERKFPFKSGVNIMYGCNNFCTYCIVPYVRGREISREPKDIIMEIERLVKDGVKEVMLLGQNVDSYG
KTLDNPVSFAQLLREIDKIEGLERIRFMTPHPKDIEEETLEVIRDSKKICNHIHFPLQSGSSRLLKLMNRKYTKEHYLEQ
VEMIRRILPDVSITTDIIVGFPGETEEDFEDTIDVVKKAKFDSAYTFIYSKRTGTPAARMENQVPEDVVKDRFNRLLATV
NEVSHEHIRRYEGMDMKVLVEGKDDHEEGFVTGRMTNNILVHFAGDESLIGQIVTVHLDECKGFYYMGRLIED

Specific function: Catalyzes the methylthiolation of N6- (dimethylallyl)adenosine (i(6)A), leading to the formation of 2- methylthio-N6-(dimethylallyl)adenosine (ms(2)i(6)A) at position 37 in tRNAs that read codons beginning with uridine [H]

COG id: COG0621

COG function: function code J; 2-methylthioadenine synthetase

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 TRAM domain [H]

Homologues:

Organism=Homo sapiens, GI28872782, Length=447, Percent_Identity=34.675615212528, Blast_Score=265, Evalue=8e-71,
Organism=Homo sapiens, GI28872784, Length=447, Percent_Identity=34.675615212528, Blast_Score=265, Evalue=9e-71,
Organism=Homo sapiens, GI93277076, Length=452, Percent_Identity=27.6548672566372, Blast_Score=155, Evalue=9e-38,
Organism=Escherichia coli, GI1786882, Length=436, Percent_Identity=39.4495412844037, Blast_Score=325, Evalue=4e-90,
Organism=Escherichia coli, GI1787057, Length=413, Percent_Identity=28.5714285714286, Blast_Score=176, Evalue=4e-45,
Organism=Caenorhabditis elegans, GI17553146, Length=435, Percent_Identity=31.264367816092, Blast_Score=230, Evalue=1e-60,
Organism=Caenorhabditis elegans, GI71996771, Length=232, Percent_Identity=33.6206896551724, Blast_Score=121, Evalue=1e-27,
Organism=Drosophila melanogaster, GI21356207, Length=479, Percent_Identity=34.8643006263048, Blast_Score=283, Evalue=2e-76,
Organism=Drosophila melanogaster, GI19922432, Length=451, Percent_Identity=27.4944567627494, Blast_Score=152, Evalue=3e-37,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006638
- InterPro:   IPR005839
- InterPro:   IPR020612
- InterPro:   IPR013848
- InterPro:   IPR006463
- InterPro:   IPR007197
- InterPro:   IPR002792 [H]

Pfam domain/function: PF04055 Radical_SAM; PF01938 TRAM; PF00919 UPF0004 [H]

EC number: NA

Molecular weight: Translated: 54801; Mature: 54801

Theoretical pI: Translated: 5.56; Mature: 5.56

Prosite motif: PS50926 TRAM ; PS01278 UPF0004

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
6.1 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
6.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQEDNNTSALEEQLYYIGECKKFLEKKTKEIGRPLYACVHTFGCQMNARDSEKLLGVLKE
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCCCCCCHHHHHHHHHH
IGYLETEDEDKSDFVIYNTCTVRENANLKVYGRLGHLKNVKRKNPHMLIAMCGCMMQEPD
CCCCCCCCCCCCCEEEEEEEEEECCCCEEEEEECHHHHHHCCCCCCEEHHHHHHHCCCCH
VVEKIRDSYKFVDIVFGTFNIYAMAKLIYNRITSGSQVIDIWEKTKDIVEELPTERKFPF
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCCCCH
KSGVNIMYGCNNFCTYCIVPYVRGREISREPKDIIMEIERLVKDGVKEVMLLGQNVDSYG
HCCCEEEECCCHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHH
KTLDNPVSFAQLLREIDKIEGLERIRFMTPHPKDIEEETLEVIRDSKKICNHIHFPLQSG
HHCCCHHHHHHHHHHHHHHCCHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCC
SSRLLKLMNRKYTKEHYLEQVEMIRRILPDVSITTDIIVGFPGETEEDFEDTIDVVKKAK
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEECCCCCHHHHHHHHHHHHHHC
FDSAYTFIYSKRTGTPAARMENQVPEDVVKDRFNRLLATVNEVSHEHIRRYEGMDMKVLV
CCCEEEEEEECCCCCCHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEE
EGKDDHEEGFVTGRMTNNILVHFAGDESLIGQIVTVHLDECKGFYYMGRLIED
ECCCCCCCCEEEEEECCCEEEEECCCHHHHHHHHHEEHHHHCCHHHHHHHHCC
>Mature Secondary Structure
MQEDNNTSALEEQLYYIGECKKFLEKKTKEIGRPLYACVHTFGCQMNARDSEKLLGVLKE
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCCCCCCHHHHHHHHHH
IGYLETEDEDKSDFVIYNTCTVRENANLKVYGRLGHLKNVKRKNPHMLIAMCGCMMQEPD
CCCCCCCCCCCCCEEEEEEEEEECCCCEEEEEECHHHHHHCCCCCCEEHHHHHHHCCCCH
VVEKIRDSYKFVDIVFGTFNIYAMAKLIYNRITSGSQVIDIWEKTKDIVEELPTERKFPF
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCCCCH
KSGVNIMYGCNNFCTYCIVPYVRGREISREPKDIIMEIERLVKDGVKEVMLLGQNVDSYG
HCCCEEEECCCHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHH
KTLDNPVSFAQLLREIDKIEGLERIRFMTPHPKDIEEETLEVIRDSKKICNHIHFPLQSG
HHCCCHHHHHHHHHHHHHHCCHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCC
SSRLLKLMNRKYTKEHYLEQVEMIRRILPDVSITTDIIVGFPGETEEDFEDTIDVVKKAK
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEECCCCCHHHHHHHHHHHHHHC
FDSAYTFIYSKRTGTPAARMENQVPEDVVKDRFNRLLATVNEVSHEHIRRYEGMDMKVLV
CCCEEEEEEECCCCCCHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEE
EGKDDHEEGFVTGRMTNNILVHFAGDESLIGQIVTVHLDECKGFYYMGRLIED
ECCCCCCCCEEEEEECCCEEEEECCCHHHHHHHHHEEHHHHCCHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA