Click here to switch to the map view.

The map label for this gene is ispH [H]

Identifier: 238916860

GI number: 238916860

Start: 944302

End: 945165

Strand: Direct

Name: ispH [H]

Synonym: EUBELI_00929

Alternate gene names: 238916860

Gene position: 944302-945165 (Clockwise)

Preceding gene: 238916859

Following gene: 238916861

Centisome position: 44.04

GC content: 38.43

Gene sequence:

>864_bases
TTGAAAGAAGTAATACTTGCAAAGTCAGCAGGCTTCTGTTTTGGTGTTCAGAGGGCAATGGATACTGTGTATGCTGAGGC
AGACAAGAAAAATGTATATACTTATGGTCCGATAATTCATAATACTGAGGTTGTCAATGAACTTGAGAGCAAGGGTGTAA
AGGCTGTTAATGATATATCTGAGATTCCAGAACCTGAAAAGTCAACTGTTATAATCAGATCTCATGGAGTATCAAAGGCA
GTATATGAATCAATAAAGAACAGCGGGGCAAAGATTGTGGATGCAACATGTCCGTTTGTATTAAAGATTCACAAGATTGT
AAAGGATGCAAGTGCTGAAGGTGACCAGATTGTAATTATTGGCAATGAGAAGCATCCTGAAGTTGAAGGCATTATGGGGT
GGTCAGAGACACCTGTACATGTTGTTGATACAGTTGAAAAAGCTGAAAAGTTGAAACTTGACAAGAGTAAAAATGTGCGT
GTGGTATCCCAGACGACATTTAATTACAAGAAATTTCAAGATTTAGTTGAAATTATAAAGAAAAAAGAGTACAATATTTG
CATTAGTAATACGATATGTAATGCAACTGAGGAGCGTCAGAACGAGGCAAGACGGATTGCTCGAAGAGCTGATGCTATGA
TTGTTATAGGGGACAGCAGTAGTTCAAACACTCGTAAACTGTATGAAATTTGCAAGACGGAATGTCAGGAAACCTTTTAC
ATTCAGACTCTTAGCGGCCTTGAGCTTGATAAGCTCGAATCATCAGACTGCATAGGTATTACAGCCGGGGCATCAACCCC
AAACAATATCATTGAGGAGGTTTATACTAATGTCAGAAGAAAGCTTTGCACAGATGTTAGATAA

Upstream 100 bases:

>100_bases
GGCAGATGATGCTGTGCTTGTTGATACATCAGATATGAATATAGAACAGGTTGTAGCTGCTTTAAGTAAGATAATAGATG
AAAAGAAGGCGGGCAGATAA

Downstream 100 bases:

>100_bases
CGAAGAATGGAAGGAAATCCGTACAGGAGAGGTGGTAGACGGAAGAGTTATTGCAGTTAAGCCAGATAGAATTGTACTTA
ATATAGGTGGCAAATCTGAT

Product: 4-hydroxy-3-methylbut-2-enyl diphosphate reductase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 287; Mature: 287

Protein sequence:

>287_residues
MKEVILAKSAGFCFGVQRAMDTVYAEADKKNVYTYGPIIHNTEVVNELESKGVKAVNDISEIPEPEKSTVIIRSHGVSKA
VYESIKNSGAKIVDATCPFVLKIHKIVKDASAEGDQIVIIGNEKHPEVEGIMGWSETPVHVVDTVEKAEKLKLDKSKNVR
VVSQTTFNYKKFQDLVEIIKKKEYNICISNTICNATEERQNEARRIARRADAMIVIGDSSSSNTRKLYEICKTECQETFY
IQTLSGLELDKLESSDCIGITAGASTPNNIIEEVYTNVRRKLCTDVR

Sequences:

>Translated_287_residues
MKEVILAKSAGFCFGVQRAMDTVYAEADKKNVYTYGPIIHNTEVVNELESKGVKAVNDISEIPEPEKSTVIIRSHGVSKA
VYESIKNSGAKIVDATCPFVLKIHKIVKDASAEGDQIVIIGNEKHPEVEGIMGWSETPVHVVDTVEKAEKLKLDKSKNVR
VVSQTTFNYKKFQDLVEIIKKKEYNICISNTICNATEERQNEARRIARRADAMIVIGDSSSSNTRKLYEICKTECQETFY
IQTLSGLELDKLESSDCIGITAGASTPNNIIEEVYTNVRRKLCTDVR
>Mature_287_residues
MKEVILAKSAGFCFGVQRAMDTVYAEADKKNVYTYGPIIHNTEVVNELESKGVKAVNDISEIPEPEKSTVIIRSHGVSKA
VYESIKNSGAKIVDATCPFVLKIHKIVKDASAEGDQIVIIGNEKHPEVEGIMGWSETPVHVVDTVEKAEKLKLDKSKNVR
VVSQTTFNYKKFQDLVEIIKKKEYNICISNTICNATEERQNEARRIARRADAMIVIGDSSSSNTRKLYEICKTECQETFY
IQTLSGLELDKLESSDCIGITAGASTPNNIIEEVYTNVRRKLCTDVR

Specific function: Converts 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate into isopentenyl diphosphate (IPP) and dimethylallyl diphosphate (DMAPP) [H]

COG id: COG0761

COG function: function code IM; Penicillin tolerance protein

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ispH family [H]

Homologues:

Organism=Escherichia coli, GI1786212, Length=289, Percent_Identity=30.7958477508651, Blast_Score=159, Evalue=2e-40,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003451 [H]

Pfam domain/function: PF02401 LYTB [H]

EC number: =1.17.1.2 [H]

Molecular weight: Translated: 31998; Mature: 31998

Theoretical pI: Translated: 6.66; Mature: 6.66

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.8 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
2.8 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKEVILAKSAGFCFGVQRAMDTVYAEADKKNVYTYGPIIHNTEVVNELESKGVKAVNDIS
CCCEEEECCCCHHHHHHHHHHHHHHHCCCCCEEEECCEECCHHHHHHHHHCCCHHHHHHH
EIPEPEKSTVIIRSHGVSKAVYESIKNSGAKIVDATCPFVLKIHKIVKDASAEGDQIVII
HCCCCCCCEEEEEECCCHHHHHHHHHCCCCEEEECCCHHHHHHHHHHHHCCCCCCEEEEE
GNEKHPEVEGIMGWSETPVHVVDTVEKAEKLKLDKSKNVRVVSQTTFNYKKFQDLVEIIK
CCCCCCCCHHCCCCCCCCHHHHHHHHHHHHHCCCCCCCEEEEEECCCCHHHHHHHHHHHH
KKEYNICISNTICNATEERQNEARRIARRADAMIVIGDSSSSNTRKLYEICKTECQETFY
HCCCCEEECCCCCCCHHHHHHHHHHHHHHCCEEEEEECCCCCCHHHHHHHHHHHHHHEEE
IQTLSGLELDKLESSDCIGITAGASTPNNIIEEVYTNVRRKLCTDVR
EEEECCCCHHHCCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MKEVILAKSAGFCFGVQRAMDTVYAEADKKNVYTYGPIIHNTEVVNELESKGVKAVNDIS
CCCEEEECCCCHHHHHHHHHHHHHHHCCCCCEEEECCEECCHHHHHHHHHCCCHHHHHHH
EIPEPEKSTVIIRSHGVSKAVYESIKNSGAKIVDATCPFVLKIHKIVKDASAEGDQIVII
HCCCCCCCEEEEEECCCHHHHHHHHHCCCCEEEECCCHHHHHHHHHHHHCCCCCCEEEEE
GNEKHPEVEGIMGWSETPVHVVDTVEKAEKLKLDKSKNVRVVSQTTFNYKKFQDLVEIIK
CCCCCCCCHHCCCCCCCCHHHHHHHHHHHHHCCCCCCCEEEEEECCCCHHHHHHHHHHHH
KKEYNICISNTICNATEERQNEARRIARRADAMIVIGDSSSSNTRKLYEICKTECQETFY
HCCCCEEECCCCCCCHHHHHHHHHHHHHHCCEEEEEECCCCCCHHHHHHHHHHHHHHEEE
IQTLSGLELDKLESSDCIGITAGASTPNNIIEEVYTNVRRKLCTDVR
EEEECCCCHHHCCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11997336 [H]